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Detergent effect on Cytochrome b559 electron paramagnetic resonance signals in the photosystem II reaction centre

Opened Access Detergent effect on Cytochrome b559 electron paramagnetic resonance signals in the photosystem II reaction centre

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Autor: Yruela, Inmaculada R
García Rubio, Inés
Roncel Gil, Mercedes
Martínez, Jesús I.
Ramiro, María Victoria
Ortega Rodríguez, José María
Alonso, Pablo Javier
Picorel, Rafael
Departamento: Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Fecha: 2003
Publicado en: Photochemical and Photobiological Sciences, 2 (4), 437-442
Tipo de documento: Artículo
Resumen: The detergent effect on Cytochrome b559 from spinach photosystem II was studied by electron paramagnetic resonance (EPR) spectroscopy in D1-D2-Cyt b559 complex preparations. Various n-dodecyl-β-D- maltoside concentrations from 0 to 0.2% (w/v) were used to stabilise the D1-D2-Cyt b559 complexes. Low spin heme EPR spectra were obtained but the g. feature positions changed depending on the detergent conditions. Redox potentiometric titrations showed a unique redox potential cytochrome b 559 form (Em′ = +123-150 mV) in all the D1-D2-Cyt b559 complex preparations indicating that detergent does not affect this property of the protein in those conditions. A similar effect on Cytochrome b559 EPR spectrum was observed in more intact photosystem II preparations independently of their aggregation state. This finding indicates that changes due to detergent could be a common phenomenon in photosystem II complexes. Results are discussed in terms of the environment each detergent provides to the pro...
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Tamaño: 140.1Kb
Formato: PDF

URI: http://hdl.handle.net/11441/40413

DOI: http://dx.doi.org/10.1039/B300187C

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