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The rkpU gene of Sinorhizobium fredii HH103 is required for bacterial K-antigen polysaccharide production and for efficient nodulation with soybean but not with cowpea

Opened Access The rkpU gene of Sinorhizobium fredii HH103 is required for bacterial K-antigen polysaccharide production and for efficient nodulation with soybean but not with cowpea

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Autor: Hidalgo, Ángeles
Margaret Oliver, Isabel María
Crespo Rivas, Juan Carlos
Parada, Maribel
Murdoch, Piedad del Socorro
López, Abigail
Buendía Clavería, Ana María
Moreno Onorato, Francisco Javier
Albareda, Marta
Gil Serrano, Antonio Miguel
Rodríguez Carvajal, Miguel Ángel
Palacios, José M.
Ruiz Sainz, José Enrique
Vinardell González, José María
Departamento: Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
Universidad de Sevilla. Departamento de Biología Celular
Universidad de Sevilla. Departamento de Química orgánica
Fecha: 2010
Publicado en: Microbiology, 156, 3398–3411
Tipo de documento: Artículo
Resumen: In this work, the role of the rkpU and rkpJ genes in the production of the K-antigen polysaccharides (KPS) and in the symbiotic capacity of Sinorhizobium fredii HH103, a broad host-range rhizobial strain able to nodulate soybean and many other legumes, was studied. The rkpJ- and rkpU-encoded products are orthologous to Escherichia coli proteins involved in capsule export. S. fredii HH103 mutant derivatives were contructed in both genes. To our knowledge, this is the first time that the role of rkpU in KPS production has been studied in rhizobia. Both rkpJ and rkpU mutants were unable to produce KPS. The rkpU derivative also showed alterations in its lipopolysaccharide (LPS). Neither KPS production nor rkpJ and rkpU expression was affected by the presence of the flavonoid genistein. Soybean (Glycine max) plants inoculated with the S. fredii HH103 rkpU and rkpJ mutants showed reduced nodulation and clear symptoms of nitrogen starvation. However, neither the rkpJ nor the rkpU m...
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Tamaño: 884.3Kb
Formato: PDF

URI: http://hdl.handle.net/11441/40310

DOI: http://dx.doi.org/10.1099/mic.0.042499-0

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