Autor: |
Muñiz Guinea, Manuel
Martín Rubio, María Esther Hidalgo Jiménez, Josefina Velasco López, Ángel |
Departamento: | Universidad de Sevilla. Departamento de Biología Celular |
Fecha: | 1997 |
Tipo de documento: | Artículo |
Resumen: |
We have examined the role played by protein
kinase A (PKA) in vesicle-mediated protein transport from the
trans-Golgi network (TGN) to the cell surface. In vivo this
transport step was inhibited by inhibitors of PKA catalytic
subunits (C-PKA) such as the compound known as H89 and a
myristoylated form of the inhibitory peptide sequence contained
in the thermostable PKA inhibitor. Inhibition by H89 occurred
at an early stage during the transfer of vesicular stomatitis virus
G glycoprotein from the TGN to the cell surface. Reversal from
this inhibition correlated with a transient increase in the number
of free coated vesicles in the Golgi area. Vesicle budding fromthe
TGN was studied in vitro using vesicular stomatitis virusinfected,
permeabilized cells. Addition to this assay of C-PKA
stimulated vesicle release while it was suppressed by PKA
inhibitory peptide, H89, and antibody against C-PKA. Furthermore,
vesicle release was decreased when PKA-depleted cytosol
was used ... [Ver más] |
Cita: | Muñiz Guinea, M., Martín Rubio, M.E., Hidalgo Jiménez, J. y Velasco, Á. (1997). Protein kinase A activity is required for the budding of constitutive transport vesicles from the trans-Golgi network. |
URI: http://hdl.handle.net/11441/40069
DOI: 10.1073/pnas.94.26.14461
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