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The retrieval function of the KDEL receptor requires PKA phosphorylation of its C-terminus

Opened Access The retrieval function of the KDEL receptor requires PKA phosphorylation of its C-terminus


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Autor: Cabrera, Margarita
Muñiz Guinea, Manuel
Hidalgo Jiménez, Josefina
Vega, Lucía
Martín, María Esther
Departamento: Universidad de Sevilla. Departamento de Biología Celular
Fecha: 2003
Publicado en: Molecular Biology of the Cell, 14 (10), 4114-4125
Tipo de documento: Artículo
Resumen: The KDEL receptor is a Golgi/intermediate compartment-located integral membrane protein that carries out the retrieval of escaped ER proteins bearing a C-terminal KDEL sequence. This occurs throughout retrograde traffic mediated by COPI-coated transport carriers. The role of the C-terminal cytoplasmic domain of the KDEL receptor in this process has been investigated. Deletion of this domain did not affect receptor subcellular localization although cells expressing this truncated form of the receptor failed to retain KDEL ligands intracellularly. Permeabilized cells incubated with ATP and GTP exhibited tubular processes-mediated redistribution from the Golgi area to the ER of the wild-type receptor, whereas the truncated form lacking the C-terminal domain remained concentrated in the Golgi. As revealed with a peptidebinding assay, this domain did not interact with both coatomer and ARF-GAP unless serine 209 was mutated to aspartic acid. In contrast, alanine replacement of serin...
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Tamaño: 844.1Kb
Formato: PDF


DOI: 10.1091/mbc.E03-04-0194

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