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Diversity of Escherichia coli Strains Producing Extended-Spectrum B-Lactamases in Spain: Second Nationwide Study


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dc.creator Díaz, Miguel A.
dc.creator Hernández-Bello, José R.
dc.creator Rodríguez-Baño, Jesús
dc.creator Martínez-Martínez, Luis
dc.creator Calvo, Jorge
dc.creator Blanco, Jorge
dc.creator Pascual Hernández, Álvaro 2016-02-18T16:07:04Z 2016-02-18T16:07:04Z 2010-08
dc.identifier.issn 0095-1137 es
dc.description.abstract The prevalence of extended-spectrum -lactamase (ESBL)-producing Escherichia coli (ESBLEC) in Spain increased 8-fold from 2000 to 2006. ESBL type, clonal relationship, antimicrobial susceptibility, and clinical data about infections caused by ESBLEC are evaluated in a second nationwide study developed in 2006. From 1008 clinical isolates obtained over 2 months from 44 hospitals, 254 were used for further analysis. ESBL production was evaluated by synergy testing, PCR, and sequencing. Antimicrobial activity was evaluated by microdilution. The clonal relationship was evaluated by repetitive extragenic palindromic-PCR (REP-PCR). The O25b subtype and the new afa operon FM955459 were determined by triplex PCR in isolates producing CTX-M-15. Multilocus sequence typing was performed on these isolates. A total of 72% of all ESBLs were of the CTX-M type, 26.8% were of the SHV type, and 1.2% were of the TEM type. The most prevalent ESBLs were CTX-M-14 (119 isolates), SHV-12 (68 isolates), CTX-M-15 (37 isolates), and CTX-M-9 (21 isolates). By REP-PCR, 214 clones were detected. All but five CTX-M-15 ESBLEC isolates corresponded to the international O25b/ST131 clone. This clone had not been detected in the first study (published in 2000). Epidemiological and clinical features were studied in 304 representative patients. A total of 60% of the patients were older than 60 and had nonfatal underlying diseases, and 55% had recently received antibiotics. Urinary tract infections accounted for 71% of cases, and 9% were bacteremic. There has been a significant increase in the prevalence of ESBLEC in Spain, with most of these strains being CTX-M-producing isolates, including the pandemic O25b-ST131. SHV-12-producing E. coli remains an important cause of community-acquired infection. es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher American Society for Microbiology es
dc.relation.ispartof Journal of Clinical Microbiology 48 (8), 2840–2845 es
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri *
dc.subject ESBL es
dc.subject ESBLEC es
dc.subject Escherichia coli es
dc.subject Extended-spectrum-lactamase es
dc.subject Antimicrobial es
dc.title Diversity of Escherichia coli Strains Producing Extended-Spectrum B-Lactamases in Spain: Second Nationwide Study es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Medicina es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Microbiología es
dc.relation.publisherversion es
dc.identifier.doi 10.1128/JCM.02147-09
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