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dc.creatorCortés Ledesma, Felipe
dc.creatorTous Rivera, Cristina
dc.creatorAguilera López, Andrés
dc.date.accessioned2015-10-01T10:01:04Z
dc.date.available2015-10-01T10:01:04Z
dc.date.issued2007
dc.identifier.issn0305-1048es
dc.identifier.issn1362-4962es
dc.identifier.urihttp://hdl.handle.net/11441/29098
dc.description.abstractHomologous recombination (HR) is the major mechanism used to repair double-strand breaks (DSBs) that result from replication, but a study of repair of DSBs specifically induced during S-phase is lacking. Using an inverted-repeat assay in which a DSB is generated by the encountering of the replication fork with nicks, we can physically detect repair by sister-chromatid recombination (SCR) and intra-chromatid break-induced replication (IC-BIR). As expected, both events depend on Rad52, but, in contrast to previous data, both require Rad59, suggesting a prominent role of Rad59 in repair of replication-born DSBs. In the absence of Rad51, SCR is severely affected while IC-BIR increases, a phenotype that is also observed in the absence of Rad54 but not of its paralog Rdh54/Tid1. These data are consistent with SCR occurring by Rad51-dependent mechanisms assisted by Rad54, and indicate that in the absence of strand exchange-dependent SCR, breaks can be channeled to IC-BIR, which works efficiently in the absence of Rad51. Our study provides molecular evidence for inversions between repeats occurring by BIR followed by single-strand annealing (SSA) in the absence of strand exchange.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherOxford University Presses
dc.relation.ispartofNucleic acids research, 35(19), 6560-6570es
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectMolecular Biologyes
dc.titleDifferent genetic requirements for repair of replication-born double-strand breaks by sister-chromatid recombination and break-induced replicationes
dc.typeinfo:eu-repo/semantics/articlees
dcterms.identifierhttps://ror.org/03yxnpp24
dc.type.versioninfo:eu-repo/semantics/publishedVersion
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Genéticaes
dc.relation.publisherversionhttp://dx.doi.org/10.1093/nar/gkm488es
dc.identifier.doihttp://dx.doi.org/10.1093/nar/gkm488es
dc.identifier.idushttps://idus.us.es/xmlui/handle/11441/29098

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