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Artículo
Role for RNA: DNA hybrids in origin-independent replication priming in a eukaryotic system
dc.creator | Stuckey, Ruth | |
dc.creator | García Rodriguez, Néstor | |
dc.creator | Aguilera López, Andrés | |
dc.creator | Wellinger, Ralf Erik | |
dc.date.accessioned | 2015-10-01T06:56:42Z | |
dc.date.available | 2015-10-01T06:56:42Z | |
dc.date.issued | 2015 | |
dc.identifier.issn | 1091-6490 | es |
dc.identifier.issn | 0027-8424 | es |
dc.identifier.uri | http://hdl.handle.net/11441/29075 | |
dc.description.abstract | DNA replication initiates at defined replication origins along eukaryotic chromosomes, ensuring complete genome duplication within a single S-phase. A key feature of replication origins is their ability to control the onset of DNA synthesis mediated by DNA polymerase-α and its intrinsic RNA primase activity. Here, we describe a novel origin-independent replication process that is mediated by transcription. RNA polymerase I transcription constraints lead to persistent RNA:DNA hybrids (R-loops) that prime replication in the ribosomal DNA locus. Our results suggest that eukaryotic genomes have developed tools to prevent R-loop–mediated replication events that potentially contribute to copy number variation, particularly relevant to carcinogenesis. | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | National Academy of Sciences | es |
dc.relation.ispartof | Proceedings of the National Academy of Sciences, 112(18), 5779-5784 | es |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | RNA:DNA hybrids | es |
dc.subject | RNase H | es |
dc.subject | topoisomerase 1 | es |
dc.subject | replication | es |
dc.subject | ribosomal DNA | es |
dc.title | Role for RNA: DNA hybrids in origin-independent replication priming in a eukaryotic system | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Genética | es |
dc.relation.publisherversion | http://dx.doi.org/10.1073/pnas.1501769112 | es |
dc.identifier.doi | http://dx.doi.org/10.1073/pnas.1501769112 | es |
dc.identifier.idus | https://idus.us.es/xmlui/handle/11441/29075 |
Ficheros | Tamaño | Formato | Ver | Descripción |
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PNAS-2015-Stuckey-5779-84.pdf | 1.143Mb | [PDF] | Ver/ | |