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Lack of Detrimental Effects of Bacillus thuringiensis Cry Toxins on the Insect Predator Chrysoperla carnea: a Toxicological, Histopathological, and Biochemical Analysis

 

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dc.creator González Zamora, José Enrique
dc.creator Rodrigo-Simon, Ana
dc.creator Maagd, Ruud A. de
dc.creator Avilla Hernández, Carlos
dc.creator Bakker, Petra L.
dc.creator Molthoff, Jos
dc.creator Ferre, Juan
dc.date.accessioned 2015-09-24T11:03:53Z
dc.date.available 2015-09-24T11:03:53Z
dc.date.issued 2006
dc.identifier.citation González Zamora, J.E., Rodrigo-Simon, A., Maagd, R.A.d., Avilla Hernández, C., Bakker, P.L., Molthoff, J. y Ferre, J. (2006). Lack of Detrimental Effects of Bacillus thuringiensis Cry Toxins on the Insect Predator Chrysoperla carnea: a Toxicological, Histopathological, and Biochemical Analysis. Applied and Environmental Microbiology, 72 (2), 1595-1603.
dc.identifier.issn 1595–1603 es
dc.identifier.uri http://hdl.handle.net/11441/28834
dc.description.abstract The effect of Cry proteins of Bacillus thuringiensis on the green lacewing (Chrysoperla carnea) was studied by using a holistic approach which consisted of independent, complementary experimental strategies. Tritrophic experiments were performed, in which lacewing larvae were fed Helicoverpa armigera larvae reared on Cry1Ac, Cry1Ab, or Cry2Ab toxins. In complementary experiments, a predetermined amount of purified Cry1Ac was directly fed to lacewing larvae. In both experiments no effects on prey utilization or fitness parameters were found. Since binding to the midgut is an indispensable step for toxicity of Cry proteins to known target insects, we hypothesized that specific binding of the Cry1A proteins should be found if the proteins were toxic to the green lacewing. In control experiments, Cry1Ac was detected bound to the midgut epithelium of intoxicated H. armigera larvae, and cell damage was observed. However, no binding or histopathological effects of the toxin were found in tissue sections of lacewing larvae. Similarly, Cry1Ab or Cry1Ac bound in a specific manner to brush border membrane vesicles from Spodoptera exigua but not to similar fractions from green lacewing larvae. The in vivo and in vitro binding results strongly suggest that the lacewing larval midgut lacks specific receptors for Cry1Ab or Cry1Ac. These results agree with those obtained in bioassays, and we concluded that the Cry toxins tested, even at concentrations higher than those expected in real-life situations, do not have a detrimental effect on the green lacewing when they are ingested either directly or through the prey. es
dc.format application/pdf es
dc.language.iso eng es
dc.publisher American Society for Microbiology es
dc.relation.ispartof Applied and Environmental Microbiology, 72 (2), 1595-1603.
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/ *
dc.title Lack of Detrimental Effects of Bacillus thuringiensis Cry Toxins on the Insect Predator Chrysoperla carnea: a Toxicological, Histopathological, and Biochemical Analysis es
dc.type info:eu-repo/semantics/article es
dc.type.version info:eu-repo/semantics/publishedVersion
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Ciencias Agroforestales es
dc.relation.publisherversion http://dx.doi.org/10.1128/AEM.72.2.1595-1603.2006
dc.identifier.doi 10.1128/AEM.72.2.1595-1603.2006 es
dc.journaltitle Applied and Environmental Microbiology
dc.publication.volumen 72 es
dc.publication.issue 2 es
dc.publication.initialPage 1595 es
dc.publication.endPage 1603 es
dc.identifier.idus https://idus.us.es/xmlui/handle/11441/28834
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