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Folate transport by prawn hepatopancreas brush-border membrane vesicles.

Opened Access Folate transport by prawn hepatopancreas brush-border membrane vesicles.

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Autor: Blaya, J. A.
Muriana, F. J. G.
Ruiz Gutiérrez, Valentina
Vázquez Cueto, Carmen María
Bolufer González, José
Departamento: Universidad de Sevilla. Departamento de Fisiología
Fecha: 1998
Publicado en: Bioscience Reports, 18(1), 9-17
Tipo de documento: Artículo
Resumen: The transport system of folic acid (Pte-Glu) b y brush-borde r membrane vesicles (BBMV ) isolated from prawn (Penaeus japonicm) hepatopancreas , was studied by measuring the uptake of Pte-Glu . This uptake was found to have two components , intravesicular transport and membrane binding . Membrane binding was not affected by the presence of a trans - membrane pH-gradient at a short incubation period . However , a transmembrane pH - gradient increased membrane binding at 6 0 min. The transpor to f Pte-Glu appeared to be carrier-mediated , was stimulated by an inwardly proton gradient (p H 5. 5 outside , 7. 4 inside ) and was unaffected by a sodium-gradient . The relationship between pH gradient-driven Pte-Glu uptake and medium Pte-Glu concentration followed saturating Michaelis-Menten kinetics . Eadie-Hofste e representation of the pH gradient-driven Pte-Glu uptake indicated a single transport system with a Km of 0.3 7 ^ Man d Vmax of 1.06pmol/mg protein/15s . These findings indicate th...
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Tamaño: 468.4Kb
Formato: PDF

URI: http://hdl.handle.net/11441/28777

DOI: http://dx.doi.org/10.1023/A:1022236616277

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