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Regulation and symbiotic significance of nodulation outer proteins secretion in Sinorhizobium fredii HH103


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dc.creator López Baena, Francisco Javier
dc.creator Vinardell González, José María
dc.creator Pérez Montaño, Francisco de Asís
dc.creator Crespo Rivas, Juan Carlos
dc.creator Bellogín Izquierdo, Ramón Andrés
dc.creator Espuny Gómez, María del Rosario
dc.creator Ollero Márquez, Francisco Javier 2015-02-25T09:56:04Z 2015-02-25T09:56:04Z 2008
dc.identifier.issn 1465-2080 es
dc.identifier.issn 1350-0872 es
dc.description.abstract In this work we show that the Sinorhizobium fredii HH103 ttsI gene is essential for the expression of the tts genes and secretion of nodulation outer proteins (Nops). Moreover, we demonstrate for the first time, to our knowledge, that the nod box preceding ttsI is necessary for Nops secretion. TtsI is responsible for the transcriptional activation of nopX, nopA, rhcJ and rhcQ. We confirm that the S. fredii HH103 ttsI gene is activated by NodD1 and repressed by NolR. In contrast, NodD2 is not involved in the regulation of ttsI expression. Despite the dependence of expression of both ttsI and nodA on NodD1 and flavonoids, clear differences in the capacity of some flavonoids to activate these genes were found. The expression of the ttsI and nodA genes was also sensitive to differences in the pH of the media. Secretion of Nops in the ttsI mutant could not be complemented with a DNA fragment containing the ttsI gene and its nod box, but it was restored when a plasmid harbouring the ttsI, rhcC2 and y4xK genes was transferred to the mutant strain. The symbiotic effect of Nops secretion was host-dependent but independent of the type of nodule formed by the host legume. Nops are beneficial in the symbiosis with Glycine max and Glycyrrhiza uralensis, and detrimental in the case of the tropical legume Erythrina variegata. es
dc.format application/pdf es
dc.language.iso eng es
dc.relation.ispartof Microbiology, 154: 1825–1836 es
dc.rights Attribution-NonCommercial-NoDerivatives 4.0 Internacional *
dc.rights.uri *
dc.title Regulation and symbiotic significance of nodulation outer proteins secretion in Sinorhizobium fredii HH103 es
dc.type info:eu-repo/semantics/article es
dc.rights.accessrights info:eu-repo/semantics/openAccess es
dc.contributor.affiliation Universidad de Sevilla. Departamento de Microbiología es
dc.relation.publisherversion 10.1099/mic.0.2007/016337-0 es
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