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Regulation and symbiotic significance of nodulation outer proteins secretion in Sinorhizobium fredii HH103

Opened Access Regulation and symbiotic significance of nodulation outer proteins secretion in Sinorhizobium fredii HH103
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Autor: López Baena, Francisco Javier
Vinardell González, José María
Pérez Montaño, Francisco
Crespo Rivas, Juan Carlos
Bellogín Izquierdo, Ramón Andrés
Espuny Gómez, María del Rosario
Ollero Márquez, Francisco Javier
Departamento: Universidad de Sevilla. Departamento de Microbiología
Fecha: 2008
Publicado en: Microbiology, 154: 1825–1836
Tipo de documento: Artículo
Resumen: In this work we show that the Sinorhizobium fredii HH103 ttsI gene is essential for the expression of the tts genes and secretion of nodulation outer proteins (Nops). Moreover, we demonstrate for the first time, to our knowledge, that the nod box preceding ttsI is necessary for Nops secretion. TtsI is responsible for the transcriptional activation of nopX, nopA, rhcJ and rhcQ. We confirm that the S. fredii HH103 ttsI gene is activated by NodD1 and repressed by NolR. In contrast, NodD2 is not involved in the regulation of ttsI expression. Despite the dependence of expression of both ttsI and nodA on NodD1 and flavonoids, clear differences in the capacity of some flavonoids to activate these genes were found. The expression of the ttsI and nodA genes was also sensitive to differences in the pH of the media. Secretion of Nops in the ttsI mutant could not be complemented with a DNA fragment containing the ttsI gene and its nod box, but it was restored when a plasmid harbouring t...
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URI: http://hdl.handle.net/11441/22757

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