Repositorio de producción científica de la Universidad de Sevilla

Rab3D Is Not Required for Exocrine Exocytosis but for Maintenance of Normally Sized Secretory Granules

 

Advanced Search
 
Opened Access Rab3D Is Not Required for Exocrine Exocytosis but for Maintenance of Normally Sized Secretory Granules
Cites
Show item statistics
Icon
Export to
Author: Riedel, Dietmar
Antonin, Wolfram
Fernández-Chacón, Rafael
Álvarez de Toledo Naranjo, Guillermo
Jo, Tobias
Geppert, Martin
Valentijn, Jack A.
Valentijn, Karin
Jamieson, James D.
Südhof, Thomas C.
Jahn, Reinhard
Department: Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica
Date: 2002
Published in: Molecular and Cellular Biology, 22 (18), 6487-6497.
Document type: Article
Abstract: Rab3D, a member of the Rab3 subfamily of the Rab/ypt GTPases, is expressed on zymogen granules in the pancreas as well as on secretory vesicles in mast cells and in the parotid gland. To shed light on the function of Rab3D, we have generated Rab3D-deficient mice. These mice are viable and have no obvious phenotypic changes. Secretion of mast cells is normal as revealed by capacitance patch clamping. Furthermore, enzyme content and overall morphology are unchanged in pancreatic and parotid acinar cells of knockout mice. Both the exocrine pancreas and the parotid gland show normal release kinetics in response to secretagogue stimulation, suggesting that Rab3D is not involved in exocytosis. However, the size of secretory granules in both the exocrine pancreas and the parotid gland is significantly increased, with the volume being doubled. We conclude that Rab3D exerts its function during granule maturation, possibly by preventing homotypic fusion of secretory granules.
Size: 590.4Kb
Format: PDF

URI: http://hdl.handle.net/11441/17818

This work is under a Creative Commons License: 
Atribución-NoComercial-SinDerivadas 4.0 España

This item appears in the following Collection(s)