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Hypoxia induces voltage-dependent Ca2+ entry and quantal dopamine secretion in carotid body glomus cells

Opened Access Hypoxia induces voltage-dependent Ca2+ entry and quantal dopamine secretion in carotid body glomus cells
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Autor: Ureña López, Juan
Fernández-Chacón, Rafael
Rodríguez Benot, Alberto
Álvarez de Toledo Naranjo, Guillermo
López Barneo, José
Departamento: Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica
Fecha: 1994
Publicado en: Proceedings of the National Academy of Sciences (nº 91, 1994, pp. 10208-10211)Proceedings of the National Academy of Sciences of the United States of America, 91 (21), 10208-10211.
Tipo de documento: Artículo
Resumen: We have investigated the changes of cytosolic [Ca2+1 and the secretory -activity in single glomus cells dispersed from rabbit carotid bodies during exposure to solutions with variable 02 tension (Po2). In normoxic conditions (Po2= 145 mmHg; 1 mmHg = 133 Pa), intracellular [Ca2+J was 58 1 29 nM, and switching to lo'iV P02 (between 10 and 60 mmHg) led to a reversible of [Ca2+1 up to 800 nM. The response to hypoxia completely disappeared after removal of external Ca2+ or with the addition of 0.2 mM Cd2+ to the external solution. These same solutions also abolished both the Ca2+ current of the cells and the increase of internal (Ca2+ elicited by hig external K+. Elevations of cytosolic [Ca2e+ in response to hypoxia or to direct membrane depolarization elicited the release of dopamine, which was detected by amperometric techniques. Dopamine secretion occurred in episodes of spike-like activity that appear to represent the release from single secretory vesicles. From the mean charge of well...
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URI: http://hdl.handle.net/11441/17476

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