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Functional and transcriptional induction of Aquaporin-1 gene by hypoxia; analysis of promoter and role of Hif-1α

Opened Access Functional and transcriptional induction of Aquaporin-1 gene by hypoxia; analysis of promoter and role of Hif-1α

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Autor: Abreu Rodríguez, Irene
Sánchez Silva, Rocío
Martins, Ana Paula
Soveral, Graça
Toledo Aral, Juan José
López Barneo, José
Echevarría Irusta, Miriam
Departamento: Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica
Fecha: 2011
Publicado en: PLoS One, 6 (12), e28385-1-e28385-12.
Tipo de documento: Artículo
Resumen: Aquaporin-1 (AQP1) is a water channel that is highly expressed in tissues with rapid O2 transport. It has been reported that this protein contributes to gas permeation (CO2, NO and O2) through the plasma membrane. We show that hypoxia increases Aqp1 mRNA and protein levels in tissues, namely mouse brain and lung, and in cultured cells, the 9L glioma cell line. Stopped-flow light-scattering experiments confirmed an increase in the water permeability of 9L cells exposed to hypoxia, supporting the view that hypoxic Aqp1 up-regulation has a functional role. To investigate the molecular mechanisms underlying this regulatory process, transcriptional regulation was studied by transient transfections of mouse endothelial cells with a 1297 bp 59 proximal Aqp1 promoter-luciferase construct. Incubation in hypoxia produced a doseand time-dependent induction of luciferase activity that was also obtained after treatments with hypoxia mimetics (DMOG and CoCl2) and by overexpressing stabilized mutate...
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Cita: Abreu Rodríguez, I., Sánchez Silva, R., Martins, A.P., Soveral, G., Toledo Aral, J.J., López Barneo, J. y Echevarría Irusta, M. (2011). Functional and transcriptional induction of Aquaporin-1 gene by hypoxia; analysis of promoter and role of Hif-1α. PLoS One, 6 (12), e28385-1-e28385-12.
Tamaño: 1.280Mb
Formato: PDF

URI: http://hdl.handle.net/11441/17470

DOI: 10.1371/journal.pone.0028385

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