Gómez-González, EmilioFernández Muñoz, BeatrizBarriga-Rivera, Alejandro2021-08-112021-08-112021-08Gómez-González, E., Fernández Muñoz, B. y Barriga-Rivera, A. [et al.] (2021). Hyperspectral image processing for the identification and quantification of lentiviral particles in fluid samples. Scientific Reports, 11, 16201.2045-2322https://hdl.handle.net/11441/116730Optical spectroscopic techniques have been commonly used to detect the presence of biofilm-forming pathogens (bacteria and fungi) in the agro-food industry. Recently, near-infrared (NIR) spectroscopy revealed that it is also possible to detect the presence of viruses in animal and vegetal tissues. Here we report a platform based on visible and NIR (VNIR) hyperspectral imaging for non-contact, reagent free detection and quantification of laboratory-engineered viral particles in fluid samples (liquid droplets and dry residue) using both partial least square-discriminant analysis and artificial feed-forward neural networks. The detection was successfully achieved in preparations of phosphate buffered solution and artificial saliva, with an equivalent pixel volume of 4 nL and lowest concentration of 800 TU·μL−1. This method constitutes an innovative approach that could be potentially used at point of care for rapid mass screening of viral infectious diseases and monitoring of the SARS-CoV-2 pandemic.application/pdf12 p.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Near-infrared spectroscopySARS-CoV-2Viral infectioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccesshttps://doi.org/10.1038/s41598-021-95756-3