Adaptive response of saccharomyces hosts to totiviridae L-A dsRNA viruses Is achieved through intrinsically balanced action of targeted transcription factors

Ravoitytė, BazilėLukša, JulianaWellinger, Ralf ErikServa, SauliusServienė, Elena2022-10-312022-10-312022Ravoitytė, B., Lukša, J., Wellinger, R.E., Serva, S. y Servienė, E. (2022). Adaptive response of saccharomyces hosts to totiviridae L-A dsRNA viruses Is achieved through intrinsically balanced action of targeted transcription factors. Journal of Fungi, 8 (4), 381. https://doi.org/10.3390/jof8040381.2309-608Xhttps://hdl.handle.net/11441/138548Totiviridae L-A virus is a widespread yeast dsRNA virus. The persistence of the L-A virus alone appears to be symptomless, but the concomitant presence of a satellite M virus provides a killer trait for the host cell. The presence of L-A dsRNA is common in laboratory, industrial, and wild yeasts, but little is known about the impact of the L-A virus on the host’s gene expression. In this work, based on high-throughput RNA sequencing data analysis, the impact of the L-A virus on whole-genome expression in three different Saccharomyces paradoxus and S. cerevisiae host strains was analyzed. In the presence of the L-A virus, moderate alterations in gene expression were detected, with the least impact on respiration-deficient cells. Remarkably, the transcriptional adaptation of essential genes was limited to genes involved in ribosome biogenesis. Transcriptional responses to L-A maintenance were, nevertheless, similar to those induced upon stress or nutrient availability. Based on these data, we further dissected yeast transcriptional regulators that, in turn, modulate the cellular L-A dsRNA levels. Our findings point to totivirus-driven fine-tuning of the transcriptional landscape in yeasts and uncover signaling pathways employed by dsRNA viruses to establish the stable, yet allegedly profitless, viral infection of fungi.application/pdf24 p.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/TotiviridaedsRNATranscriptionyeastSaccharomyces cerevisiaeSaccharomyces paradoxusAdaptive response of saccharomyces hosts to totiviridae L-A dsRNA viruses Is achieved through intrinsically balanced action of targeted transcription factorsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccesshttps://doi.org/10.3390/jof8040381