Maldonado y Aibar, María DoloresMoreno-García, H.González Yanes, CarmenCalvo Gutiérrez, Juan Ramón2022-05-182022-05-182016-01-08Maldonado, M. D., García-Moreno, H., González-Yanes, C., & Calvo, J. R. (2016). Possible Involvement of the Inhibition of NF-κB Factor in Anti-Inflammatory Actions That Melatonin Exerts on Mast Cells. Journal of cellular biochemistry, 117(8), 1926–19331097-4644https://hdl.handle.net/11441/133444Melatonin is a molecule endogenously produced in a wide variety of immune cells, including mast cells (RBL-2H3). It exhibits immunomodulatory, anti-inflammatory and anti-apoptotic properties. The physiologic mechanisms underlying these activities of melatonin have not been clarified in mast cells. This work is designed to determine the anti-inflammatory effect and mechanism of action of melatonin on activated mast cells. RBL-2H3 were pre-treated with exogenous melatonin (MELx) at physiological (100nM) and pharmacological (1mM) doses for 30 min, washed and activated with PMACI (phorbol 12-myristate 13-acetate plus calcium ionophore A23187) for 2h and 12h. The data shows that pre-treatment of MELx in stimulated mast cells, significantly reduced the levels of endogenous melatonin production (MELn), TNF-alpha and IL-6. These effects are directly related with the MELx concentration used. MELx also inhibited IKK/NF-kB signal transduction pathway in stimulated mast cells. These results indicate a molecular basis for the ability of melatonin to prevent inflammation and for the treatment of allergic inflammatory diseases through the down-regulation of mast cell activation.application/pdf26 p.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/MelatoninRBL-2H3 mast cellsCytokinesNF-kBInflammationinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccesshttps://doi.org/10.1002/jcb.25491