Castillejo, AnaMartínez Muñoz, GuillermoDelgado-Pujol, Ernesto J.Villalobo Polo, EduardoCarrillo de la Fuente, FranciscoCasado Jurado, DavidPérez Bernal, Juan LuisBegines Ruiz, BelénTorres Hernández, YadirAlcudia Cruz, Ana2025-02-062025-02-062025-04Castillejo, A., Martínez Muñoz, G., Delgado-Pujol, E.J., Villalobo Polo, E., Carrillo de la Fuente, F., Casado Jurado, D.,...,Alcudia Cruz, A. (2025). Enhanced porous titanium biofunctionalization based on novel silver nanoparticles and nanohydroxyapatite chitosan coatings. International Journal of Biological Macromolecules, 299, 139846. https://doi.org/10.1016/j.ijbiomac.2025.139846.0141-81301879-0003https://hdl.handle.net/11441/168351Titanium is widely used for implants however it presents limitations such as infection risk, stress shielding phenomenon, and poor osseointegration. To address these issues, a novel approach was proposed that involves fabricating porous titanium substrates, to reduce implant stiffness, minimizing stress shielding and bone resorption, and applying polymeric coatings to improve bioactivity. Composite coating prepared from chitosan, silver nanoparticles, and nanohydroxyapatite was optimized to enhance antibacterial properties and promote osseointegration. Chitosan with 80.5 % of deacetylation degree was used to prepare composites with diverse compositions, including different methodologies of adding silver nanoparticles, with silver concentrations below toxic level. Antibacterial activity was tested with three different strains, including Gram+ and Gram− bacteria, demonstrating excellent inhibition after 21 days. In addition, the induction of hydroxyapatite formation was investigated. Finally, the optimal porous metallic substrate that exhibited a more suitable stiffness (29 GPa) (close to the cortical bone tissue they intend to replace) was chosen to be infiltrated with the selected composites. In summary, this synergistic approach based on the combination of porous titanium substrates with 60 vol% porosity and a 355–500 μm pore size distribution coated with 3%CS-nHA-AgNPs-TPP-AgNPsbath composite provided a potential solution to provide implants with improved biomechanical balance and biofunctionality.application/pdf37 p.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Biodegradable polymersChitosanHydrogelHydroxyapatitePorous TiTi implantsSilver nanoparticlesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccesshttps://doi.org/10.1016/j.ijbiomac.2025.139846