2024-07-172024-07-172017Velázquez Cayón, R.T., Castillo-Dalí, G., Corcuera Flores, J.R., Serrera Figallo, M.d.l.Á., Castillo-Oyagüe, R., González Martín, M.I.,...,Torres-Lagares, D. (2017). Production of bone mineral material and BMP-2 in osteoblasts cultured on double acid-etched titanium. Medicina oral, patología oral y cirugía bucal, 22 (5), e651-e659. https://doi.org/10.4317/medoral.22071.1698-44471698-6946https://hdl.handle.net/11441/161451Background: The study of osteoblasts and their osteogenic functions is essential in order to understand them and their applications in implantology. In this sense, this study try to study BMP-2 production and bone matrix de position, in addition to other biological variables, in osteoblasts cultured on a rough double acid-etched titanium surface (Osseotite®, Biomet 3i, Palm Beach Garden, Florida, USA) in comparison to a smooth titanium surface (machined) and a control Petri dish. Material and Methods: An in vitro prospective study. NHOst human osteoblasts from the femur were cultured on three different surfaces: Control group: 25-mm methacrylate dish (n = 6); Machined group: titanium discs with machined surface (n = 6) and Experimental group: titanium discs with a double acid-etched nitric and hydrofluoric Osseotite® acid surface (n = 6). A quantification of the mitochondrial membrane potential, and studies of apopto sis, mobility and adhesion, bone productivity (BMP-2) and cellular bone synthesis were carried out after culturing the three groups for forty-eight hours. Results: A statistically significant difference was observed in the production of BMP-2 between the experimental group and the other two groups (22.33% ± 11.06 vs. 13.10% ± 5.51 in the machined group and 3.88% ± 3.43 in the control group). Differences in cellular bone synthesis were also observed between the groups (28.34% ± 14.4% in the experimental group vs. 20.03% ± 6.79 in the machined group and 19.34% ± 15.93% in the control group). Conclusions: In comparison with machined surfaces, Osseotite® surfaces favor BMP-2 production and bone syn thesis as a result of the osteoblasts in contact with it.application/pdf9 p.engBMP-2CytoskeletonCell cultureBone matrixApoptosisCell viabilityinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess10.4317/medoral.22071