2025-05-142025-05-142025Casas Rodríguez, A., Cascajosa Lira, A., Puerto Rodríguez, M., Cameán Fernández, A.M. y Jos Gallego, Á.M. (2025). In silico and in vitro evaluation of potential agonistic and antagonistic estrogenic and androgenic activities of pure cyanotoxins, microcystin-LR and cylindrospermopsin. Ecotoxicology and environmental Safety, 289, 117456. https://doi.org/10.1016/j.ecoenv.2024.117456.1090-24140147-6513https://hdl.handle.net/11441/172705The potential endocrine disruption activity of cyanotoxins, particularly their effects on estrogen and androgen receptors (ER, AR), remains poorly understood. In the present study, the potential agonistic/antagonistic estrogenic and androgenic activities of MC-LR and CYN have been determined for the first time with validated OECD Test Guidelines No. 455 and 458, respectively. The data show that only MC-LR demonstrated weak estrogenic agonistic effects (LogPC10 value of − 9.85 M), while both toxins displayed antagonistic effects on the ER, with LogIC30 values of − 4.4 and − 6.4 for MC-LR and CYN, respectively. In addition, neither MC-LR nor CYN exhibited agonistic/antagonistic activities in AR. Docking studies revealed potential interactions between both toxins and AR, with CYN showing a higher predicted affinity for this receptor. In vivo studies, particularly those investigating androgen disruption, are warranted to confirm the endocrine disrupting potential of MC-LR and CYN.application/pdf10engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/MC-LRCYNEstrogensAndrogensDockingIn silico and in vitro evaluation of potential agonistic and antagonistic estrogenic and androgenic activities of pure cyanotoxins, microcystin-LR and cylindrospermopsininfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccesshttps://doi.org/10.1016/j.ecoenv.2024.117456