Zhou, TianXu, ZeqianSun, HaishuiBeltrán, Ana M.Nawaz, QaisarSui, BaiyanBoccaccini, Aldo R.Zheng, Kai2024-05-102024-05-102024-050927-77571873-4359https://hdl.handle.net/11441/158048Iron (Fe) is a crucial element in the human body, playing a significant role in bone metabolism. The release of Fe ions at bone defect sites can promote bone regeneration. In this study, we synthesized Fe-containing mesoporous bioactive glasses (Fe-MBGs) in SiO₂-CaO-Fe₂O₃ composition using a sol-gel method. Regardless of the amount of incorporated Fe₂O₃ (up to 5 mol%), the Fe-MBGs maintained a mesoporous structure, and the inclusion of Fe₂O₃ did not alter their amorphous characteristics. However, the presence of Fe₂O₃ led to a reduction in both pore volume and specific surface area of the Fe-MBGs. Notably, Fe-MBGs demonstrated degradability in physiological fluids and could sustain release of Si, Fe, and Ca ions. Higher concentrations of incorporated Fe₂O₃ were found to reduce the degradation of Fe-MBGs. All Fe-MBGs exhibited favorable bioactivity, as evidenced by the rapid formation of hydroxyapatite when exposed to simulated body fluid. Fe-MBGs also demonstrated concentration-dependent effects on BMSCs and Saos-2 cells. Extracts of Fe-MBGs at 0.1 and 1 mg/mL exhibited non-cytotoxicity and promoted cell proliferation. Additionally, extracts of Fe-MBGs at 1 mg/mL significantly enhanced the alkaline phosphatase activity of BMSCs and Saos-2 cells, along with an upregulation of the expression of osteogenesis-related genes. These findings unlock the significant potential of Fe-MBGs as functional biomaterials for bone regeneration applications. The controlled release of Fe ions from these mesoporous bioactive glasses orchestrates osteogenic differentiation in bone marrow mesenchymal stem cells and osteoblasts.application/pdf31 p.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Mesoporous bioactive glassesIronOsteogenic differentiationBone regenerationinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/embargoedAccesshttps://doi.org/10.1016/j.colsurfa.2024.134188