Toledano, ManuelAsady, SamaraToledano-Osorio, ManuelGarcía-Godoy, FranklinSerrera Figallo, María de los ÁngelesBenítez-García, José A.Osorio, Raquel2023-07-062023-07-062020Toledano, M., Asady, S., Toledano-Osorio, M., García-Godoy, F., Serrera Figallo, M.d.l.Á., Benítez-García, J.A. y Osorio, R. (2020). Differential biodegradation kinetics of collagen membranes for bone regeneration. Polymers, 12 (6), 1290. https://doi.org/10.3390/POLYM12061290.2073-4360https://hdl.handle.net/11441/147771Native collagen-based membranes are used to guide bone regeneration; but due to their rapid biodegradation, this treatment is often unpredictable. The purpose of this study was to investigate the biodegradability of natural collagen membranes. Three non-cross-linked resorbable collagen barrier membranes were tested: Derma Fina (porcine dermis), Evolution Standard (equine pericardium) and Duo-Teck (equine lyophilized collagen felt). 10 × 10 mm2 pieces of membranes were submitted to three different degradation procedures: (1) hydrolytic degradation in phosphate buffer solution, (2) enzyme resistance, using a 0.25% porcine trypsin solution, and (3) bacterial (Clostridium histolyticum) collagenase resistance test. Weight measurements were performed with an analytic microbalance. Thickness was measured with a digital caliper. Membranes were analyzed at different time-points, up to 21 d of immersion. A stereomicroscope was used to obtain membranes’ images. ANOVA and Student Newman Keuls were used for mean comparisons (p < 0.05), except when analyzing differences between time-points within the same membrane and solution where pair-wise comparisons were applied (p < 0.001). Derma Fina attained the highest resistance to all degradation challenges. Duo-Teck was the most susceptible membrane to degradation, complete degradation occurred as soon as 8 h. The bacterial collagenase solution performed as the most aggressive test as all membranes presented 100% degradation before 21 d.application/pdf17 pág.engAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/CollagenMembraneBiodegradationBone regenerationinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccesshttps://doi.org/10.3390/POLYM12061290