López Cantillo, MónicaVidal Veuthey, BorisMella, ArminRuiz de la Haba, RafaelCollado, Luis2024-01-162024-01-162023López Cantillo, M., Vidal Veuthey, B., Mella, A., Ruiz de la Haba, R. y Collado, L. (2023). Helicobacter ibis sp. nov., isolated from faecal droppings of black-faced ibis (Theristicus melanopis). International Journal of Systematic and Evolutionary Microbiology, 73 (11), 005983. https://doi.org/10.1099/ijsem.0.005983.1466-50261466-5034https://hdl.handle.net/11441/153479As part of a larger study on Epsilonproteobacteria carried by wild birds in the city of Valdivia (southern Chile), two curved rod-shaped Gram-stain-negative strains (A82T and WB-40) were recovered from faecal samples and subjected to a taxonomic study. Results of a genus-specific PCR showed that these isolates belonged to the genus Helicobacter. Further identification by 16S rRNA and hsp60 (60 kDa heat-shock protein) gene sequence analysis revealed that they formed a separate phylogenetic clade, different from other known Helicobacter species with ‘Helicobacter burdigaliensis’ CNRCH 2005/566HT and Helicobacter valdiviensis WBE14T being the most closely related species. This was confirmed by core-genome phylogeny as well as digital DNA–DNA hybridization and average nucleotide identity analyses between the genomes of strains A82T and WB-40 and all other Helicobacter species. The draft genome sequences of A82T and WB-40, obtained by Illumina NextSeq 2000 sequencing, consisted of 1.6 Mb with a G+C content of 31.9–32.0 mol%. The results obtained from the phylogenetic and genomic characteri-zation, together with their different morphological and biochemical features, revealed that these two strains represent a novel species, for which we propose the name Helicobacter ibis sp. nov. with A82T (=LMG 32718T=CCCT 22.04T) as the type strain.application/pdf20 p.engAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/ChileFaecesHelicobacterWild birdinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccesshttps://doi.org/10.1099/ijsem.0.005983