2023-11-142023-11-142023Buzón, P., Velázquez Cruz, A., Corrales Guerrero, L., Díaz Quintana, A.J., Díaz Moreno, I. y Roos, W.H. (2023). The Histone Chaperones SET/TAF-1β and NPM1 Exhibit Conserved Functionality in Nucleosome Remodeling and Histone Eviction in a Cytochrome c-Dependent Manner. Advanced Science, 10 (29), 2301859. https://doi.org/10.1002/advs.202301859.2198-3844https://hdl.handle.net/11441/150659Chromatin homeostasis mediates essential processes in eukaryotes, where histone chaperones have emerged as major regulatory factors during DNA replication, repair, and transcription. The dynamic nature of these processes, however, has severely impeded their characterization at the molecular level. Here, fluorescence optical tweezers are applied to follow histone chaperone dynamics in real time. The molecular action of SET/template-activating factor-Iβ and nucleophosmin 1—representing the two most common histone chaperone folds—are examined using both nucleosomes and isolated histones. It is shown that these chaperones present binding specificity for fully dismantled nucleosomes and are able to recognize and disrupt non-native histone-DNA interactions. Furthermore, the histone eviction process and its modulation by cytochrome c are scrutinized. This approach shows that despite the different structures of these chaperones, they present conserved modes of action mediating nucleosome remodeling.application/pdf12 p.engAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/Fluorescence microscopyNucleophosmin 1Optical tweezersSET/template-activating factor-IβSingle-molecule methodsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess10.1002/advs.202301859