Horra Padilla, Carmen de laCano Rodríguez, María MercedesPeral Rubio, María JoséGarcía Delgado, MartaDurán Martínez, Juan ManuelCalonge Castrillo, María LuisaIlundáin Larrañeta, María Anunciación Ana2020-04-242020-04-242001Horra Padilla, C.d.l., Cano Rodríguez, M.M., Peral Rubio, M.J., García Delgado, M., Durán Martínez, J.M., Calonge Castrillo, M.L. y Ilundáin Larrañeta, M.A.A. (2001). Na+-dependent D-mannose transport at the apical membrane of rat small intestine and kidney cortex. Biochimica et Biophysica Acta - Biomembranes, 1512 (2), 225-230.0005-27361879-2642https://hdl.handle.net/11441/95724The presence of a Na+/D-mannose cotransport activity in brush-border membrane vesicles (BBMV), isolated from either rat small intestine or rat kidney cortex, is examined. In the presence of an electrochemical Na+ gradient, but not in its absence, D-mannose was transiently accumulated by the BBMV. D-Mannose uptake into the BBMV was energized by both the electrical membrane potential and the Na+ chemical gradient. D-Mannose transport vs. external D-mannose concentration can be described by an equation that represents a superposition of a saturable component and another component that cannot be saturated up to 50 μM D-mannose. D-Mannose uptake was inhibited by D-mannose ≫ D-glucose > phlorizin, whereas for α-methyl glucopyranoside the order was D-glucose = phlorizin ≫ D-mannose. The initial rate of D-mannose uptake increased as the extravesicular Na+ concentration increased, with a Hill coefficient of 1, suggesting that the Na+ :D-mannose cotransport stoichiometry is 1:1. It is concluded that both rat intestinal and renal apical membrane have a concentrative, saturable, electrogenic and Na+-dependent D-mannose transport mechanism, which is different from SGLT1.application/pdf6 p.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Brush-border membrane vesicleIntestineKidneySodium/D-mannoseNa+-dependent D-mannose transport at the apical membrane of rat small intestine and kidney cortexinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccesshttps://doi.org/10.1016/S0005-2736(01)00322-4