2017-03-082017-03-082014Medina, D.A., Jordán Pla, A., Millán Zambrano, G., Chávez de Diego, S. y Pérez Ortín, .E. (2014). Cytoplasmic 5′-3′ exonuclease Xrn1p is also a genome-wide transcription factor in yeast. Frontiers in genetics, 5 (1), 1-10.1664-8021http://hdl.handle.net/11441/55590The 5′ to 3′ exoribonuclease Xrn1 is a large protein involved in cytoplasmatic mRNA degradation as a critical component of the major decaysome. Its deletion in the yeast Saccharomyces cerevisiae is not lethal, but it has multiple physiological effects. In a previous study, our group showed that deletion of all tested components of the yeast major decaysome, including XRN1, results in a decrease in the synthetic rate and an increase in half-life of most mRNAs in a compensatory manner. Furthermore, the same study showed that the all tested decaysome components are also nuclear proteins that bind to the 5′ region of a number of genes. In the present work, we show that disruption of Xrn1 activity preferentially affects both the synthesis and decay of a distinct subpopulation of mRNAs. The most affected mRNAs are the transcripts of the highly transcribed genes, mainly those encoding ribosome biogenesis and translation factors. Previously, we proposed that synthegradases play a key role in regulating both mRNA synthesis and degradation. Evidently, Xrn1 functions as a synthegradase, whose selectivity might help coordinating the expression of the protein synthetic machinery. We propose to name the most affected genes “Xrn1 synthegradon.”application/pdfengAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/transcription ratenascent transcriptionmRNA synthesismRNA decaymRNA stabilityinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess10.3389/fgene.2014.00001