2024-11-212024-11-212021-05-14Pérez Alfonso, D., López López, M., López-Cornejo, M.d.P., Romero Azogil, L., Benito Hernández, E.M., García Martín, M.d.G.,...,Moyá Morán, M.L. (2021). Properties of polyplexes formed between a cationic polymer derived from l-arabinitol and nucleic acids. New Journal of Chemistry, 45 (22), 10098-10108. https://doi.org/10.1039/d1nj00606a.1144-05461369-9261https://hdl.handle.net/11441/164753In this work a sugar-based cationic polymer derived from L-arabinitol, PUArab, was prepared and its interactions with the linear calf thymus DNA and with the circular plasmid pEGFP-C1 were investigated at different N/P ratios. The polyplexes were characterized by using several techniques. For both nucleic acids, a charge inversion was observed, together with a conformational change from a coiled structure to a more compacted one. However, the N/P ratio required to observe the DNA condensation depended on the nucleic acid architecture. PUArab presents low toxicity in several cell lines. The transfection efficiency, TE, of the PUArab/pEGFP-C1 polyplexes was investigated at several N/P ratios in order to study their potential as vectors in gene transfection.application/pdf11 p.enginfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess10.1039/d1nj00606a