Ortiz Cerda, Tamara AndreaArgüelles Arias, FedericoBegines Ruiz, BelénGarcía Montes, Josefa MaríaPereira Yáñez, AlejandraVictoriano Rojas, MontserratVázquez Román, María VictoriaPérez Bernal, Juan LuisCallejón Fernández, Raquel MaríaMiguel Rodríguez, Manuel deAlcudia Cruz, Ana2021-06-282021-06-282021-05Ortiz Cerda, T.A., Argüelles Arias, F., Begines Ruiz, B., García Montes, J.M., Pereira Yáñez, A., Victoriano Rojas, M.,...,Alcudia Cruz, A. (2021). Native Chilean Berries Preservation and In Vitro Studies of a Polyphenol Highly Antioxidant Extract from Maqui as a Potential Agent against Inflammatory Diseases. Antioxidants, 10 (6), 843.2076-3921https://hdl.handle.net/11441/114908The best conservation method for native Chilean berries has been investigated in combination with an implemented large-scale extract of maqui berry, rich in total polyphenols and anthocyanin to be tested in intestinal epithelial and immune cells. The methanolic extract was obtained from lyophilized and analyzed maqui berries using Folin–Ciocalteu to quantify the total polyphenol content, as well as 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC) to measure the antioxidant capacity. Determination of maqui’s anthocyanins profile was performed by ultra-high-performance liquid chromatography (UHPLC-MS/MS). Viability, cytotoxicity, and percent oxidation in epithelial colon cells (HT-29) and macrophages cells (RAW 264.7) were evaluated. In conclusion, preservation studies confirmed that the maqui properties and composition in fresh or frozen conditions are preserved and a more efficient and convenient extraction methodology was achieved. In vitro studies of epithelial cells have shown that this extract has a powerful antioxidant strength exhibiting a dose-dependent behavior. When lipopolysaccharide (LPS)-macrophages were activated, noncytotoxic effects were observed, and a relationship between oxidative stress and inflammation response was demonstrated. The maqui extract along with 5-aminosalicylic acid (5-ASA) have a synergistic effect. All of the compiled data pointed out to the use of this extract as a potential nutraceutical agent with physiological benefits for the treatment of inflammatory bowel disease (IBD).application/pdf17 p.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Maqui berry extractPreservation methodsAntioxidant activityPolyphenols and anthocyanins contentOxidative stressInflammationRAW 264.7 cellsHT-29 cellsInflammatory bowel diseaseinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccesshttps://doi.org/10.3390/antiox10060843