Medrano Padial, ConcepciónPuerto Rodríguez, MaríaMoreno Onorato, Francisco JavierRichard, TristanCantos Villar, EmmaPichardo Sánchez, Silvia2019-11-072019-11-072019Medrano Padial, C., Puerto Rodríguez, M., Moreno Onorato, F.J., Richard, T., Cantos Villar, E. y Pichardo Sánchez, S. (2019). In Vitro Toxicity Assessment of Stilbene Extract for Its Potential Use as Antioxidant in the Wine Industry. Antioxidants, 8 (10 art. 467), 1-16.2076-3921https://hdl.handle.net/11441/90065The reduction of sulfur dioxide in wine is a consumer’s demand, considering the allergic effects that may occur in people who are sensitive to it. Stilbenes are candidates of great interest for this purpose because of their antioxidant/antimicrobial activities and health properties, and also because they are naturally found in the grapevine. In the present study, the in vitro toxicity of an extract from grapevine shoots (with a stilbene richness of 45.4%) was assessed in two human cell lines. Significant damage was observed from 30 μg/mL after 24 h, and 40 µg/mL after 48 h of exposure. Similarly, the ultrastructural study revealed a significant impairment of cell growing. The extract was able to protect cells against an induced oxidative stress at all concentrations studied. In view of the promising results, a more exhaustive toxicological assessment of the extract is needed to confirm the safety of its further use as additive in wine.application/pdfengAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Caco-2Hep-G2antioxidantcytotoxicitystilbenewineinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccesshttps://doi.org/10.3390/antiox8100467