Montserrat de la Paz, SergioBermúdez Pulgarín, BeatrizLópez Martín, SergioNaranjo, Maria C.Romero, YolandaBando Hidalgo, María J.Abia González, María del RocíoGarcía Muriana, Francisco José2023-01-252023-01-252017Montserrat de la Paz, S., Bermúdez Pulgarín, B., López Martín, S., Naranjo, M.C., Romero, Y., Bando Hidalgo, M.J.,...,García Muriana, F.J. (2017). Exogenous fatty acids and niacin on acute prostaglandin D-2 production in human myeloid cells. Journal of Nutritional Biochemistry, 39, 22-31. https://doi.org/10.1016/j.jnutbio.2016.09.007.0955-28631873-4847https://hdl.handle.net/11441/141850Niacin activates HCA2 receptor that results in the release of PGD2. However, little is known on PGD2-producing cells and the role of fatty acids. Notably M-CSF macrophages exhibited a timely dependent PGD2 production upon niacin challenge. Short pretreatment of M-CSF macrophages with autologous postprandial TRLs induced the down-regulation of HCA2 gene and up-regulation of genes encoding COX1 and COX2 enzymes in a fatty acid-dependent manner. These effects were paralleled by a higher PGD2 production with postprandial TRL-SFAs. The niacin-mediated transcriptional activity of all genes involved in PGD2 biosynthesis was desensitized in a time-dependent manner by postprandial TRLs, leading to a decreased PGD2 release. In vivo, the net excursions of PGD2 in plasma followed similar fatty acid-dependent patterns as those found for PGD2 release in vitro. The predominant fatty acid class in the diet acutely modulates PGD2 biosynthetic pathway both in vitro and in vivo.application/pdf10 p.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/NiacinExogenous fatty acidsPostprandial stateProstaglandin D2Myeloid cellsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccesshttps://doi.org/10.1016/j.jnutbio.2016.09.007