Pacios, OlgaFernández García, LauraBleriot, InésBlasco, LucíaPascual Hernández, ÁlvaroTomás, María2022-03-182022-03-182021-12-31Pacios, O., Fernández García, L., Bleriot, I., Blasco, L., Pascual Hernández, Á. y Tomás, M. (2021). Phenotypic and Genomic Comparison of Klebsiella pneumoniae Lytic Phages: vB_KpnM-VAC66 and vB_KpnM-VAC13. Viruses, 14 (1)1999-4915https://hdl.handle.net/11441/131012Klebsiella pneumoniae is a human pathogen that worsens the prognosis of many immuno compromised patients. Here, we annotated and compared the genomes of two lytic phages that infect clinical strains of K. pneumoniae (vB_KpnM-VAC13 and vB_KpnM-VAC66) and phenotypically char acterized vB_KpnM-VAC66 (time of adsorption of 12 min, burst size of 31.49 ± 0.61 PFU/infected cell, and a host range of 20.8% of the tested strains). Transmission electronic microscopy showed that vB_KpnM-VAC66 belongs to the Myoviridae family. The genomic analysis of the phage vB_KpnM VAC66 revealed that its genome encoded 289 proteins. When compared to the genome of vB_KpnM VAC13, they showed a nucleotide similarity of 97.56%, with a 93% of query cover, and the phylo genetic study performed with other Tevenvirinae phages showed a close common ancestor. How ever, there were 21 coding sequences which differed. Interestingly, the main differences were that vB_KpnM-VAC66 encoded 10 more homing endonucleases than vB_KpnM-VAC13, and that the nu cleotidic and amino-acid sequences of the L-shaped tail fiber protein were highly dissimilar, leading to different three-dimensional protein predictions. Both phages differed significantly in their host range. These viruses may be useful in the development of alternative therapies to antibiotics or as a co-therapy increasing its antimicrobial potential, especially when addressing multidrug resistant (MDR) pathogens.application/pdf16 p.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Lytic phagesKlebsiella pneumoniaeGenomic annotationHoming endonucleasesL-shaped tail fiberinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccesshttps://doi.org/10.3390/v14010006