2022-04-192022-04-192002Garriga Román, C., Vázquez Cueto, C.M., Ruiz Gutiérrez, V. y Planas Rosselló, J. (2002). Regional differences in transport, lipid composition, and fluidity of apical membranes of small intestine of chicken. Poultry Science, 81 (4), 537-545.0032-57911525-3171https://hdl.handle.net/11441/132146Na+-dependent D-glucose transport was studied in brush-border membrane vesicles from duodenum, jejunum, and ileum of 5- to 6-wk-old chickens. Regional differences were found, and both initial rates and accumulation ratio of D-glucose were higher in the proximal part of the small intestine than in the ileum. To establish the mechanism(s) underlying these differences we have studied the density of Na+-dependent D-glucose cotransporter (SGLT1) as well as lipid composition and fluidity. Phlorizin-specific binding and Western blot analysis indicated a decrease in the amount of SGLT1 in the ileum when compared to the duodenum and jejunum. The distal part of the small intestine also showed a decrease in free cholesterol content and saturated-to-unsaturated fatty acid ratio together with an increase in lipid content and phosphatidylcholine-to-sphingomyelin ratio. These results were associated with a decrease in the diphenylhextriene fluorescence polarization found in brush-border membranes of the ileum. We can conclude that the decrease in the apical D-glucose transport found in the ileum is primarily due to a reduction in the amount of SGLT1 present in the brush-border membrane rather than the differences in the lipid composition and fluidity.application/pdf9 p.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/brush-border membrane vesiclessodium-glucose transporter isoform 1phospholipidfatty acidcholesterolinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess10.1093/ps/81.4.537