2023-01-042023-01-0420190016-2361https://hdl.handle.net/11441/140918Enzymatic hydrolysis is recognised as an effective pre-treatment for increasing biodegradability of sludge. In this work, isolated commercial enzymes as well as in-situ enzymes producer bacteria were used respectively as enhancers and pre-treatments of sewage sludge. Biodegradability of sample as well as biomethane potential production were studied. Results showed that depuration efficiencies in terms of CODs (73.5–85.5%) and TVS (28.5–42.7%) were more than twice the control value. In addition, pre-treated samples as well as enhanced samples with enzymes generated more biomethane than control. The optimal ones, were those with the isolated proteases (P) and with bacteria (Bacillus licheniformis) treatment in-situ (F), producing a total volume of 72.4 ± 2.62 ml CH4 and 114 ml ± 0.46 CH4, respectively, increasing the biogas volume in 3.65 and 5.77 times respectively compared with control.application/pdf6 p.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Sewage sludgeAnaerobic digestion enhancerSewage sludge fermentationEnzymatic pre-treatmentBMPinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess10.1016/j.fuel.2019.115713