Martín Díaz, AndreaVega, Clara deMartín Hernanz, SaraAparicio Martínez, AbelardoGonzález Albaladejo, Rafael2025-06-182025-06-182025-03-27Martín Díaz, A., Vega, C.d., Martín Hernanz, S., Aparicio Martínez, A. y González Albaladejo, R. (2025). De novo transcriptome assembly of the plant Helianthemum marifolium for the study of adaptive mechanisms. Scientific Data, 12 (1), 515. https://doi.org/10.1038/s41597-025-04888-y.2052-4463https://hdl.handle.net/11441/174496The genus Helianthemum, commonly known as rockroses, encompasses 140 species primarily,distributed in the Palearctic region, with notable diversifcation driven by climatic and geological,changes. These plants are valuable for studying speciation processes and ecological divergence.,The chemical properties of the leaves have also been investigated for containing valuable bioactive,compounds with several therapeutic properties. However, the availability of genomic resources,for species in this genus are almost entirely lacking. Here, we assembled and annotated the frst,reference transcriptome of Helianthemum marifolium, a species with wide morphological variability,and infraspecifc diversity. Illumina paired-end RNA sequences were generated using leaves from,16 individuals, representing the four recognized subspecies, all cultivated in a greenhouse. RNA reads,were assembled with Trinity and Oases, and EvidentialGene produced a transcriptome with 122,002,transcripts. The transcriptome showed 59524 hits on the UniProtBK database through BLASTx. This,transcriptome will be an invaluable resource for transcriptome-level population studies, conservation,genetics of the many endangered species within the genus, and for deepen into the metabolic pathways,of leaf-derived compounds.application/pdf6 p.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccesshttps://doi.org/10.1038/s41597-025-04888-y