2019-04-012019-04-012014Olloqui Sariego, J.L., Moreno Beltrán, J.B., Díaz Quintana, A.J., Rosa Acosta, M.Á.d.l., Calvente Pacheco, J.J. y Andreu Fondacabe, R.J. (2014). Temperature-driven changeover in the electron-transfer mechanism of a thermophilic plastocyanin. Journal of Physical Chemistry Letters, 5 (5), 910-914.1948-7185https://hdl.handle.net/11441/84985Electron-transfer kinetics of the thermophilic protein Plastocyanin fromPhormidium laminosumadsorbed on 1,ω-alkanedithiol self-assembled monolayers (SAMs)deposited on gold have been investigated. The standard electron-transfer rate constant hasbeen determined as a function of electrode−protein distance and solution viscosity over abroad temperature range (0−90°C). For either thin or thick SAMs, the electron-transferregime remains invariant with temperature, whereas for the 1,11-undecanethiol SAM ofintermediate chain length, a kinetic regime changeover from a gated or friction-controlledmechanism at low temperature (0−30°C) to a nonadiabatic mechanism above 40°Cisobserved. To the best of our knowledge, this is thefirst time a thermal-induced transitionbetween these two kinetic regimes is reported for a metalloprotein.application/pdfengAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Thermophilic plastocyaninProtein electron transferKinetic regime changeover1,ω-alkanedithiol monolayerTemperatureSolution viscosityinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess10.1021/jz500150y