González Zamora, José EnriqueCastillo, María LuzAvilla Hernández, Carlos2025-01-162025-01-162024-01-102025-01-16González Zamora, J.E., Castillo, M.L. y Avilla Hernández, C. (2025). Side effects of different pesticides used in citrus on the adult stage of the parasitoid Aphytis melinus DeBach (Hymenoptera Aphelinidae) and its progeny [Dataset]. idUS (Depósito de Investigación de la Universidad de Sevilla). https://doi.org/10.12795/11441/166817.https://hdl.handle.net/11441/166817Data obtained during the years 2010 and 2011 on the effect of 12 insecticides commonly used in citrus on the parasitoid Aphytis melinus. The insecticides were spinosad, azadirachtin, fenbutatin, fosetyl-Al, copper oxichloride, mancozeb, Pirimicarb, pyriproxifen, paraffinic oil, abamectin, chlorpyrifos, and lambda-cyhalothrin The objective was to determine their effects on parasitoid survival and fecundity, and the duration of the residue of each pesticide. The fresh residue assay was performed by spraying Petri dishes at laboratory concentrations using a Potter tower. Six to eight A. melinus adults were placed on each plate. Mortality was assessed at 24 hours. Surviving females of each Petri dish were transferred to a correspondent ventilated container with a piece of squash with drops of honey and an excess of Aspidiotus nerii scales. Females were left with the scales for 48-72 h to parasitize the hosts offered, and the resulting parasitoid offspring were counted and sexed following their emergence. A similar methodology was used to assess mortality using 7-day residuals. The most toxic products found in the previous assays were selected to study their residue degradation over time. This experiment was an extended laboratory test, where products were applied with a portable sprayer on young cv. Navelina orange trees, and the evaluation of residue persistence was made underlaboratory conditions. Three trees were sprayed with each pesticide at the maximum recommended field rate. Mortality was evaluated 1, 2, 4, 6, 9 and 11 weeks after the insecticide treatment, collecting leaves from the trees and placing them in Petri dishes where A. melinus adults were introduced. Six of these pesticides were found to be harmless to moderately harmful to this parasitoid in a laboratory assay in closed Petri dishes: spinosad (bait formulation), azadirachtin, fenbutatin, fosetyl-Al, copper oxichloride, and mancozeb, with their scores on the reduction of beneficial capacity (RBC) index being between 21.4 and 94.6% after one week. The other six pesticides classified as harmful were tested on citrus plants to study their persistence over time under greenhouse conditions: Pirimicarb, pyriproxifen, paraffinic oil, abamectin, chlorpyrifos, and lambda-cyhalothrin. Most of these products reduced their negative effect on adults of A. melinus between one and six weeks after treatment, although lambdacyhalothrin was still harmful to parasitoids 11 weeks after application The files contain data on parasitoid survival and fecundity of the different assays. A detail of the materials and methods can be found in the publication itself.- Ensayo_Fresco_csv, donde se encuentra los resultados del ensayo sobre residuo fresco. - Ensayo_7dias, donde se encuentra los resultados del ensayo sobre residuo de 7 días. - Ensayo_Persistencia_csv, donde se encuentra los resultados del ensayo de persistencia.text/csvengAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/FecundityIPMmortalityparasitismpersistenceinfo:eu-repo/semantics/datasetinfo:eu-repo/semantics/openAccesshttps://doi.org/10.12795/11441/166817