2022-03-142022-03-142017López Martín, S., Montserrat de la Paz, S., Lucas Rodríguez, R., Bermúdez Pulgarín, B., Abia González, M.d.R., Morales, J.C. y García Muriana, F.J. (2017). Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cells. Journal of Functional Foods, 29, 238-247.1756-4646https://hdl.handle.net/11441/130762The effects of chemically synthesized metabolites (sulfate and glucuronate forms) from hydroxytyrosol (HTyr) on oxidative stress and inflammation were investigated in TNF-α-activated human endothelial cells. HTyr sulfate metabolites decreased reactive oxygen species and prevented the decrease in glutathione, glutathione peroxidase 1, and glutamate-cysteine ligase catalytic subunit and up-regulated heme oxygenase-1 levels. HTyr and all tested HTyr metabolites (HTyr sulfate > HTyr glucuronate > HTyr) suppressed the phosphorylation of nuclear factor kappa B proteins, the gene expression of intercellular and vascular adhesion molecules, E-selectin, chemokine (CC) motif ligand 2, and prostaglandin-endoperoxidase synthase 2 and the adhesion of human monocytes. In addition, HTyr sulfate metabolites suppressed plantar and ear swelling and myeloperoxidase activity in inflamed ear tissue in mice treated with carrageenan or 12-O-tetradecanoylphorbol-13-acetate. This study demonstrates the antioxidant and/or anti-inflammatory properties of HTyr metabolites in TNF-α-activated hECs and in the prevention of acute and chronic inflammation in mice.application/pdf50 p.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Endothelial cellsHumanHydroxytyrosolInflammationMetabolitesMiceinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess10.1016/j.jff.2016.12.033