2025-05-212025-05-212025-05Gutiérrez Gutiérrez, B., Gallego-Mesa, B., Kaasch, A.J., Riediger, M., Rieg, S., Trigo, M.,...,Rodríguez-Baño, J. (2025). Identification and validation of clinical phenotypes in Staphylococcus aureus bloodstream infection and their association with mortality (FEN-AUREUS study). EClinicalMedicine, 83, 103240. https://doi.org/10.1016/j.eclinm.2025.103240.2589-5370https://hdl.handle.net/11441/173071Background Staphylococcus aureus bacteraemia (SAB) is heterogeneous in patients and infection-related features. The aim of the study was to identify clinical phenotypes among patients with SAB, to evaluate their association with mortality, and to derive and validate a simplified probabilistic model for phenotypes assignment. Methods Phenotypes were derived using two-stage cluster analysis of 2128 patients from the ISAC cohort (recruited between 2013 and 2015), analysing 62 variables. Cox regression assessed phenotype–mortality associations. Logistic regression was employed to develop a simplified probabilistic model for sub-phenotype allocation, validated in two external international cohorts: INSTINCT (1217 patients, recruited between 2006 and 2011) and FEN-AUREUS (1185 patients, recruited between January 2021 and October 2024). The association between sub-phenotypes and 30-day mortality in the validation cohorts was also assessed.application/pdf11 p.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Staphylococcus aureusPhenotypesMortalityBacteraemiaClinical profilesinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess10.1016/j.eclinm.2025.103240