2021-09-072021-09-072021Gutiérrez Beltrán, E., Elander, P.H., Dalman, K., Dayhoff, G.W.I., Moschou, P.N., Uversky, V.N.,...,Bozhkov, P.V. (2021). Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis. EMBO Journal, 40 (17), e105043.0261-41891460-2075https://hdl.handle.net/11441/125556Tudor staphylococcal nuclease (TSN; also known as Tudor-SN, p100, or SND1) is a multifunctional, evolutionarily conserved regulator of gene expression, exhibiting cytoprotective activity in animals and plants and oncogenic activity in mammals. During stress, TSN stably associates with stress granules (SGs), in a poorly understood process. Here, we show that in the model plant Arabidopsis thaliana, TSN is an intrinsically disordered protein (IDP) acting as a scaffold for a large pool of other IDPs, enriched for conserved stress granule components as well as novel or plant-specific SG-localized proteins. While approximately 30% of TSN interactors are recruited to stress granules de novo upon stress perception, 70% form a protein–protein interaction network present before the onset of stress. Finally, we demonstrate that TSN and stress granule formation promote heat-induced activation of the evolutionarily conserved energy-sensing SNF1-related protein kinase 1 (SnRK1), the plant orthologue of mammalian AMP-activated protein kinase (AMPK). Our results establish TSN as a docking platform for stress granule proteins, with an important role in stress signalling.application/pdf21 p.engAttribution-NonCommercial-NoDerivatives 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Heat stressIntrinsically disordered regionsSnRK1/SNF1/AMPKStress granulesTudor staphylococcal nucleaseinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess10.15252/embj.2020105043