Montserrat de la Paz, SergioVillanueva Lazo, ÁlvaroMillan, FranciscoMartín Santiago, VictoriaRivero Pino, FernandoMillán Linares, María del Carmen2025-01-162025-01-162023-12Montserrat de la Paz, S., Villanueva Lazo, Á., Millan, F., Martín Santiago, V., Rivero Pino, F. y Millán Linares, M.d.C. (2023). Production and identification of immunomodulatory peptides in intestine cells obtained from hemp industrial by-products. Food research international, 174 (Pt 1), 113616. https://doi.org/10.1016/j.foodres.2023.113616.0963-99691873-7145https://hdl.handle.net/11441/166828Hemp seeds have attracted the interest of the food industry recently, to be employed as functional food, considering their nutritional composition, highlighting the high content and quality of the proteins. In this study, ten hemp protein hydrolysates (HPHs) were obtained by enzymatic hydrolysis with two food-grade proteases from a hemp protein isolate and the inflammatory properties were evaluated in Caco-2 cell line. To this end, the gene expression and the release of proinflammatory and anti-inflammatory cytokines by Caco-2 cells stimulated with bacterial lipopolysaccharide and treated with HPHs at concentrations of 50 and 100 μg/mL were analyzed. The peptides contained in each HPH were identified and those with higher quality of the match in the spectrum were subjected to in silico analyses to determine which peptides were bioactive and contributing to the immunomodulatory activity of the hydrolysates. The results suggest that the immunomodulatory properties of these HPHs could have a beneficial effect at the level of the intestinal epithelium. The HPH20A and HPH60A + 15F exerted high immunomodulatory properties based on the cytokine levels release. The oligopeptides MAEKEGFEWVSF and GLHLPSYTNTPQLVYIVK were proposed as the most active ones. The potential of these peptides as nutraceuticals to prevent or pretreat intestinal inflammation is promising, though requires validation by in vivo assays.application/pdfengAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Bioactive oligopeptidesBy-productsCannabis sativa.info:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccesshttps://doi.org/10.1016/j.foodres.2023.113616