2025-04-242025-04-242025Pérez-Matute, P., Castillo Manzano, J.I., Yuste Bielsa, S., Íñiguez, M. y Motilva, M.J. (2025). Exploring biomarkers of regular wine consumption in human urine: Targeted and untargeted metabolomics approaches. Food Chemistry, 469, 142128. https://doi.org//10.1016/j.foodchem.2024.142128.0308-81461873-7072https://hdl.handle.net/11441/172020The epidemiological assessment of wine consumption usually has been obtained using self-reporting questionnaires. In this study, two metabolomic approaches, targeted and untargeted, were applied to 24-h urine samples from a cohort of La Rioja (Spain) (aged 52–78), comparing moderate and daily wine consumers (20 males and 13 females) without diet intervention, versus non-consumers (8 males and 35 females). Results showed that the nontargeted metabolomics approach has allowed for the annotation of sixteen compounds in 24-h urine samples from regular wine-consumers that were not detected in the urine of non-wine consumers. Additionally, the targeted metabolomics approach showed a wide range of phenol metabolites, mainly hepatic phase-II conjugates, whose concentration was significantly higher in the urine of wine consumers. As a novelty, this study focuses on discovering the main urinary biomarkers of regular wine consumption involving free-living volunteers, without dietary intervention or restrictions that might alter their regular behaviors and lifestylesapplication/pdfengAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/Wine phenol metabolitesTargeted metabolomicsUrine metabolomeUntargeted metabolomicsWine consumption biomarkersinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccess/10.1016/j.foodchem.2024.142128