Bayona Feliu, AleixHerrera Moyano, EmiliaBadra Fajardo, NibalGalván Femenía, IvánSoler Oliva, María EugeniaAguilera López, Andrés2023-11-242023-11-242023Bayona Feliu, A., Herrera Moyano, E., Badra Fajardo, N., Galván Femenía, I., Soler Oliva, M.E. y Aguilera López, A. (2023). The chromatin network helps prevent cancer-associated mutagenesis at transcription-replication conflicts. Nature Communications, 14 (1), 6890. https://doi.org/10.1038/s41467-023-42653-0.2041-1723https://hdl.handle.net/11441/151567Genome instability is a feature of cancer cells, transcription being an important source of DNA damage. This is in large part associated with R-loops, which hamper replication, especially at head-on transcription-replication conflicts (TRCs). Here we show that TRCs trigger a DNA Damage Response (DDR) involving the chromatin network to prevent genome instability. Depletion of the key chromatin factors INO80, SMARCA5 and MTA2 results in TRCs, fork stalling and R-loop-mediated DNA damage which mostly accumulates at S/G2, while histone H3 Ser10 phosphorylation, a mark of chromatin compaction, is enriched at TRCs. Strikingly, TRC regions show increased mutagenesis in cancer cells with signatures of homologous recombination deficiency, transcription-coupled nucleotide excision repair (TC-NER) and of the AID/ APOBEC cytidine deaminases, being predominant at head-on collisions. Thus, our results support that the chromatin network prevents R-loops and TRCs from genomic instability and mutagenic signatures frequently associated with cancer.application/pdf16 p.engAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccesshttps://doi.org/10.1038/s41467-023-42653-0