2024-03-29T05:57:32Zhttps://idus.us.es/oai/requestoai:idus.us.es:11441/1391572024-02-14T19:27:27Zcom_11441_10989com_11441_10983com_11441_10690com_11441_10999com_11441_11044col_11441_10990col_11441_11000col_11441_11045
00925njm 22002777a 4500
dc
2021
Leptin is an important regulator of basal metabolism and food intake, with a pivotal role in obesity. Leptin exerts many different actions on various tissues and systems, including cancer, and is considered as a linkage between metabolism and the immune system. During the last decades, obesity and leptin have been associated with the initiation, proliferation and progression of many types of cancer. Obesity is also linked with complications and mortality, irrespective of the therapy used, affecting clinical outcomes. However, some evidence has suggested its beneficial role, called the “obesity paradox”, and the possible antitumoral role of leptin. Recent data regarding the immunotherapy of cancer have revealed that overweight leads to a more effective response and leptin may probably be involved in this beneficial process. Since leptin is a positive modulator of both the innate and the adaptive immune system, it may contribute to the increased immune response stimulated by immunotherapy in cancer patients and may be proposed as a good actor in cancer. Our purpose is to review this dual role of leptin in cancer, as well as trying to clarify the future perspectives of this adipokine, which further highlights its importance as a cornerstone of the immunometabolism in oncology.
Jiménez Cortegana, C., López-Saavedra, A., Sánchez Jiménez, F., Pérez Pérez, A., Castiñeiras Fernández, J., Virizuela-Echaburu, J.A.,...,Sánchez Margalet, V. (2021). Leptin, both bad and good actor in cancer. https://doi.org/10.3390/biom11060913.
2218-273X(electrónico)
https://hdl.handle.net/11441/139157
10.3390/biom11060913
leptin
Obesity
Inflammation
Cancer
Immune system
Immunotherapy
Leptin, both bad and good actor in cancer
oai:idus.us.es:11441/246772024-02-14T09:12:25Zcom_11441_11100com_11441_11099com_11441_10690com_11441_10989com_11441_10983com_11441_11115com_11441_11321com_11441_11290com_11441_11230com_11441_10757com_11441_10691com_11441_11064com_11441_2781com_11441_2379com_11441_137148col_11441_11102col_11441_10990col_11441_11116col_11441_11322col_11441_11231col_11441_10758col_11441_11065col_11441_2799
00925njm 22002777a 4500
dc
2001
Morales Lozano, J.A., Villar Angulo, L.M., Betancourt Serna, F., Caro González, F.J., Casanueva Rocha, C., Correa Manfredi, J.,...,Periáñez Cristóbal, R. (2001). Metaetnografía, sentido pedagógico de actos cotidianos. Revista de Enseñanza Universitaria, 17, 77-136.
1132-8983
http://institucional.us.es/revistas/universitaria/17/art%204.pdf
http://hdl.handle.net/11441/24677
https://idus.us.es/xmlui/handle/11441/24677
Metaetnografía, sentido pedagógico de actos cotidianos
oai:idus.us.es:11441/1536532024-02-13T21:56:44Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2019
The formation of misfolded protein oligomers during early stages of amyloid aggregation and the activation of neuroinflammatory responses are two key events associated with neurodegenerative diseases. Although it has been established that misfolded oligomers are involved in the neuroinflammatory process, the links between their structural features and their functional effects on the immune response remain unknown. To explore such links, we took advantage of two structurally distinct soluble oligomers (type A and B) of protein HypF-N and compared the elicited microglial inflammatory responses. By using confocal microscopy, protein pull-down, and high-throughput mass spectrometry, we found that, even though both types bound to a common pool of microglial proteins, type B oligomers—with a lower solvent-exposed hydrophobicity—showed enhanced protein binding, correlating with the observed inflammatory response. Furthermore, the interactome associated with inflammatory-mediated neurodegeneration revealed previously unidentified receptors and signaling molecules likely to be involved in the oligomer-elicited innate immune response.
Mannini, B., Vecchi, G., Labrador Garrido, A., Fabre, B., Pozo Pérez, D., Dobson, C.M., Roodveldt, C. y Roodveldt, C. (2019). Differential Interactome and Innate Immune Response Activation of Two Structurally Distinct Misfolded Protein Oligomers. ACS Chemical Neuroscience, 18 (8), 3464-3478. https://doi.org/0.1021/acschemneuro.9b00088..
1948-7193
https://hdl.handle.net/11441/153653
0.1021/acschemneuro.9b00088.
Misfolded oligomers
protein misfolding
neuroinflammation
neurodegenerative disease
innate immunity
molecular mechanisms
Differential Interactome and Innate Immune Response Activation of Two Structurally Distinct Misfolded Protein Oligomers
oai:idus.us.es:11441/632042024-02-17T16:32:17Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2014-07-30
This paper summarizes key emerging issues in fragile X-associated tremor/ataxia syndrome (FXTAS) as presented at the First International Conference on the FMR1 Premutation: Basic Mechanisms & Clinical Involvement in 2013.
Hall, D.A., Birch, R.C., Anheim, M., Jønch, A.E., Pintado Sanjuán, E., O’Keefe, J.,...,Leehey, a.M. (2014). Emerging topics in FXTAS. Journal of Neurodevelopmental Disorders, 6
18661947
http://hdl.handle.net/11441/63204
10.1186/1866-1955-6-31
FMR1
FXTAS
Premutation
Fragile X
Tremor
Ataxia
Emerging topics in FXTAS
oai:idus.us.es:11441/174402024-02-13T22:10:25Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2008
Pozo Pérez, D. (2008). Immune-based disorders: the challenges for translational immunology. Journal of Cellular and Molecular Medicine, 12 (4), 1085-1086.
1582-1838
http://hdl.handle.net/11441/17440
https://idus.us.es/xmlui/handle/11441/17440
Immune-based disorders: the challenges for translational immunology
oai:idus.us.es:11441/1517782024-02-14T13:32:55Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2021
Our aim was to assess the combination of olive tree-related extracts with the most favorable profile of in vitro bioactive properties. We tested the antioxidant (increment of low-density lipoprotein resistance against oxidation), vasoactive (promotion of nitric oxide release and decrease of endothelin-1 production in human umbilical vein endothelial cells), anti-inflammatory (decrease of the endothelial production of vascular cell adhesion molecule-1), and antithrombotic (reduction of the endothelial release of plasminogen activator inhibitor-1) capacities of six phenolic extracts and three triterpenic acid solutions (Ps and Ts, respectively). We tested extracts alone and in combination, at nutritional (Ps: 0.05–0.5 μmol/L; Ts: 0.001–0.1 μmol/L) and nutraceutical doses (Ps: 1–10 μmol/L; Ts: 0.25–10 μmol/L). The combination of Ps rich in 3,4-dihydroxyphenylglycol (76%, P2), hydroxytyrosol (95%, P3), and oleuropein (70%, P4) (final nutritional concentration: 0.15 μmol/L; final nutraceutical concentration: 3 μmol/L) was the best in order to prepare functional products and nutraceuticals with cardioprotective properties, despite the fact that the isolated extract with the greatest in vitro properties was P5 (75% oleocanthal), suggesting a potential synergistic effect among different olive components.
Hernáez, Á., Jaramillo, S., Garcia-Borrego, A., Espejo-Calvo, J.A., Covas, M.I., Blanchart, G.,...,Fitó, M. (2021). From Green Technology to Functional Olive Oils: Assessing the Best Combination of Olive Tree-Related Extracts with Complementary Bioactivities. Antioxidants, 10 (2), 202. https://doi.org/10.3390/antiox10020202.
2076-3921
https://hdl.handle.net/11441/151778
10.3390/antiox10020202
Olive tree
Phenolic compound
Triterpenes
Bioactivity
Functional olive oil
3,4-dihydroxyphenylglycol
Hydroxytyrosol
Oleuropein
Oleocanthal
From Green Technology to Functional Olive Oils: Assessing the Best Combination of Olive Tree-Related Extracts with Complementary Bioactivities
oai:idus.us.es:11441/1395612024-02-13T09:03:31Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2022
Obesity is a growing worldwide health problem, affecting many people due to excessive saturated fat consumption, lack of exercise, or a sedentary lifestyle. Leptin is an adipokine secreted by adipose tissue that increases in obesity and has central actions not only at the hypothalamic level but also in other regions and nuclei of the central nervous system (CNS) such as the cerebral cortex and hippocampus. These regions express the long form of leptin receptor LepRb, which is the unique leptin receptor capable of transmitting complete leptin signaling, and are the first regions to be affected by chronic neurocognitive deficits, such as mild cognitive impairment (MCI) and Alzheimer’s Disease (AD). In this review, we discuss different leptin resistance mechanisms that could be implicated in increasing the risk of developing AD, as leptin resistance is frequently associated with obesity, which is a chronic low-grade inflammatory state, and obesity is considered a risk factor for AD. Key players of leptin resistance are SOCS3, PTP1B, and TCPTP whose signalling is related to inflammation and could be worsened in AD. However, some data are controversial, and it is necessary to further investigate the underlying mechanisms of the AD-causing pathological processes and how altered leptin signalling affects such processes.
Flores Cordero, J.A., Pérez Pérez, A., Jiménez Cortegana, C., Alba Jiménez, G., Flores-Barragán, A. y Sánchez Margalet, V. (2022). Obesity as a Risk Factor for Dementia and Alzheimer’s Disease: The Role of Leptin. International Journal of Molecular Sciences, 23 (9), 5202. https://doi.org/10.3390/ijms23095202.
1422-0067
https://hdl.handle.net/11441/139561
10.3390/ijms23095202
Obesity
Leptin
Inflammation
Leptin resistance
Alzheimer’s disease
Obesity as a Risk Factor for Dementia and Alzheimer’s Disease: The Role of Leptin
oai:idus.us.es:11441/1516202024-02-13T08:45:13Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2021
The exchange of cerebrospinal (CSF) and interstitial fluid is believed to be vital for waste clearance in the brain. The sleep-dependent glymphatic system, which is comprised of perivascular flow of CSF and is largely dependent on arterial pulsatility and astrocytic aquaporin-4 (AQP4) expression, facilitates much of this brain clearance. During the last decade, several observations have indicated that impaired glymphatic function goes hand in hand with neurodegenerative diseases. Since pathologies of the brain carry inflammatory components, we wanted to know how acute inflammation, e.g., with lipopolysaccharide (LPS) injections, would affect the glymphatic system. In this study, we aim to measure the effect of LPS on perivascular CSF distribution as a measure of glymphatic function.
1742-2094
https://hdl.handle.net/11441/151620
10.1186/s12974-021-02082-6
Acute systemic
LPS-exposure
Perivascular CSF
Mice
Acute systemic LPS-exposure impairs perivascular CSF distribution in mice
oai:idus.us.es:11441/1539432024-01-24T17:52:21Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2021
Olea europaea L. leaves constitute a source of bioactive compounds with recognized benefits
for both human health and technological purposes. In the present work, different extracts from olive
leaves were obtained by the application of two extraction methods, Soxhlet and microwave-assisted
extraction (MAE), and six solvents (distilled water, ethanolic and glycerol mixtures solvents). MAE
was applied under 40, 60 and 80 ◦C for 3, 6.5 and 10 min. The effect of the extraction method, solvent
and treatment factors (the latter in MAE) on the total phenol content (TPC), the antioxidant activity
(AA) and the phenolic profile of the extracts were all evaluated. The extracts showed high values of
TPC (up to 76.1 mg GAE/g DW) and AA (up to 78 mg TE/g DW), with oleuropein being the most
predominant compound in all extracts. The Soxhlet extraction method exhibited better yields in TPC
than in MAE, although both methods presented comparable AA values. The water MAE extract
presented the strongest antimicrobial activity against five foodborne pathogens, with minimum
inhibitory concentration (MIC) values ranging from 2.5 to 60 mg/mL. MAE water extract is proposed
to be exploited in the food and nutraceutical industry in the frame of a sustainable economy
Sánchez-Gutiérrez, M., Bascón-Villegas, I., Rodríguez, A., Pérez-Rodríguez, F. y Fernández Prior, Á. (2021). Valorisation of Olea europaea L. Olive Leaves through the Evaluation of Their Extracts: Antioxidant and Antimicrobial Activity. food, 10 (5), 966. https://doi.org/10.3390/foods10050966.
2304-8158
https://hdl.handle.net/11441/153943
10.3390/foods10050966
Agri-food waste
Bioactive compounds
Polyphenols
Foodborne pathogens
Microwave
Assisted extraction
Nutraceutical
Valorisation of Olea europaea L. Olive Leaves through the Evaluation of Their Extracts: Antioxidant and Antimicrobial Activity
oai:idus.us.es:11441/1090912024-02-14T11:45:05Zcom_11441_10989com_11441_10983com_11441_10690com_11441_10999col_11441_10990col_11441_11000
00925njm 22002777a 4500
dc
2018
Leptin is now considered an important signalling molecule of the reproductive system, as it regulates the production of gonadotrophins, the blastocyst formation and implantation, the normal placentation, as well as the foeto-placental communication. Leptin is a peptide hormone secreted
mainly by adipose tissue, and the placenta is the second leptin-producing tissue in humans. Placental leptin is an important cytokine which regulates placental functions in an autocrine or paracrine manner. Leptin seems to play a crucial role during the first stages of pregnancy as it modulates critical processes such as proliferation, protein synthesis, invasion and apoptosis in placental cells. Furthermore, deregulation of leptin levels
has been correlated with the pathogenesis of various disorders associated with reproduction and gestation, including polycystic ovary syndrome,
recurrent miscarriage, gestational diabetes mellitus, pre-eclampsia and intrauterine growth restriction. Due to the relevant incidence of the mentioned diseases and the importance of leptin, we decided to review the latest information available about leptin action in normal and pathological
pregnancies to support the idea of leptin as an important factor and/or predictor of diverse disorders associated with reproduction and pregnancy
Pérez Pérez, A., Toro, A., Vilariño García, T., Maymó, J.L., Guadix, P., Varone, C. y Sánchez Margalet, V. (2018). Leptin action in normal and pathological pregnancies. Journal Of Cellular And Molecular Medicine, 22 (2), 716-727.
1582-1838
https://hdl.handle.net/11441/109091
Leptin
Reproduction
Placenta
Polycystic ovary syndrome
Recurrent miscarriage
Pre-eclampsia
Gestational diabetes
Growth restriction
Leptin action in normal and pathological pregnancies
oai:idus.us.es:11441/1251592024-02-15T07:45:14Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11034col_11441_10990col_11441_11035
00925njm 22002777a 4500
dc
2019-05-20
Background: Mast cells (MCs) in the brain can respond to environmental cues and relay signals to neurons that
may directly influence neuronal electrical activity, calcium signaling, and neurotransmission. MCs also express
receptors for neurotransmitters and consequently can be activated by them. Here, we developed a coculture model
of peritoneal MCs, incubated together with dissociated hippocampal neurons for the study of cellular mechanisms
involved in the mast cell-neuron interactions.
Methods: Calcium imaging was used to simultaneously record changes in intracellular calcium [Ca2+]i in neurons
and MCs. To provide insight into the contribution of MCs on neurotransmitter release in rat hippocampal neurons,
we used analysis of FM dye release, evoked by a cocktail of mediators from MCs stimulated by heat.
Results: Bidirectional communication is set up between MCs and hippocampal neurons. Neuronal depolarization
caused intracellular calcium [Ca2+]i oscillations in MCs that produced a quick response in neurons. Furthermore,
activation of MCs with antigen or the secretagogue compound 48/80 also resulted in a neuronal [Ca2+]i response.
Moreover, local application onto neurons of the MC mediator cocktail elicited Ca2+ transients and a synaptic release
associated with FM dye destaining. Neuronal response was partially blocked by D-APV, a N-methyl-D-aspartate
receptor (NMDAR) antagonist, and was inhibited when the cocktail was pre-digested with chondroitinase ABC,
which induces enzymatic removal of proteoglycans of chondroitin sulfate (CS).
Conclusions: MC-hippocampal neuron interaction affects neuronal [Ca2+]i and exocytosis signaling through a
NMDAR-dependent mechanism.
Flores Cordero, J.A., Ramírez-Ponce, M.P., Montes Fernández, M.d.l.Á., Balseiro Gómez, S., Acosta López, J., Álvarez de Toledo, G. y Alés González de la Higuera, E.M. (2019). Proteoglycans involved in bidirectional communication between mast cells and hippocampal neurons. Journal of Neuroinflammation, 16 (1), art.n.107.
1742-2094
https://hdl.handle.net/11441/125159
10.1186/s12974-019-1504-6
Neuro-immune
Hippocampal neurotransmission
NMDA receptors
Proteoglycans
Exocytosis
Proteoglycans involved in bidirectional communication between mast cells and hippocampal neurons
oai:idus.us.es:11441/1306232022-03-09T14:55:28Zcom_11441_10813com_11441_10802com_11441_10690com_11441_10989com_11441_10983com_11441_11084col_11441_10814col_11441_10990col_11441_11085
00925njm 22002777a 4500
dc
2017
Tyrosol (Tyr) is a phenolic compound found in virgin olive oil. After ingestion, Tyr undergoes extensive first pass intestinal/hepatic metabolism. However, knowledge about the biological effects of Tyr metabolites is scarce. We chemically synthesized Tyr glucuronate (Tyr-GLU) and sulphate (Tyr-SUL) metabolites and explored their properties against oxidative stress and inflammation in TNF-α-treated human umbilical vein endothelial cells (hECs). Tyr and Tyr-SUL prevented the rise of reactive oxygen species, the depletion of glutathione, and the down-regulation of glutathione peroxidase 1, glutamate-cysteine ligase catalytic subunit, and heme oxygenase-1 genes. Tyr-SUL and to a lower extent Tyr and Tyr-GLU prevented the phosphorylation of NF-κB signaling proteins. Tyr-GLU and Tyr-SUL also prevented the over-expression of adhesion molecules at gene, protein, and secretory levels, and the adhesion (Tyr-SUL > Tyr-GLU) of human monocytes to hECs. In vivo, Tyr, and most notably Tyr-SUL in a dose-dependent manner, ameliorated plantar and ear edemas in mice models of acute and chronic inflammation. This study demonstrates the antioxidant and/or anti-inflammatory properties of Tyr metabolites, with Tyr-SUL being the most effective.
García Muriana, F.J., Montserrat de la Paz, S., Lucas Rodríguez, R., Bermúdez Pulgarín, B., Jaramillo Carmona, S., Morales, J.C.,...,López Martín, S. (2017). Tyrosol and its metabolites as antioxidative and anti-inflammatory molecules in human endothelial cells. Food and Function, 8 (8), 2905-2914.
2042-6496
2042-650X
https://hdl.handle.net/11441/130623
10.1039/C7FO00641A
Tyrosol and its metabolites as antioxidative and anti-inflammatory molecules in human endothelial cells
oai:idus.us.es:11441/1376482024-02-13T21:56:02Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11044col_11441_10990col_11441_11045
00925njm 22002777a 4500
dc
2021-09-15
The search of prognostic factors is a priority in diffuse large B-cell lymphoma (DLBCL)
due to its aggressiveness. We have recently found that the level of circulating MDSCs is a good
marker of survival in a translational study based on a trial (EudraCT Number: 2014-001620-29), using
lenalidomide combined with R-GDP (rituximab plus gemcitabine, cisplatin, and dexamethasone).
Since Vitamin D is a known immunomodulator, we have studied blood levels of these cell populations
comparing patients with deficit of vitamin D levels (<15 ng/mL with those with normal levels
>15 ng/mL. Mann–Whitney U test was used to compare cells distributions between groups, Wilcoxon
test to compare cells distribution at different times and Spearman test to measure the association
between cell populations. Patients with vitamin D deficit maintained the increased level of immune
suppressor cells, whereas we observed a depletion of all immune suppressor cells in patients with
normal vitamin D levels. In conclusion, we have confirmed the importance of vitamin D in the
response to treatment in R/R DLBCL, suggesting that vitamin D deficit may be involved in the
immune deficit of these patients, and thus, vitamin D supplementation in these patients may help to
obtain a better response, warranting further investigation.
Jiménez Cortegana, C., Sánchez Martínez, P.M., Palazón Carrión, N., Nogales Fernández, E., Henao Carrasco, F.M., García Sancho, A.M.,...,Sánchez Margalet, V. (2021). Lower Survival and Increased Circulating Suppressor Cells in Patients with Relapsed/Refractory Diffuse Large B-Cell Lymphoma with Deficit of Vitamin D Levels Using R-GDP Plus Lenalidomide (R2-GDP): Results from the R2-GDP-GOTEL Trial. Cancers, 13 (18), 4622. https://doi.org/10.3390/cancers13184622.
2072-6694
https://hdl.handle.net/11441/137648
10.3390/cancers13184622
Diffuse large B-cell lymphoma
Immune system
MDSC
Treg
PD-1
Vitamin D
Immuno-suppression
Lenalidomid
Lower Survival and Increased Circulating Suppressor Cells in Patients with Relapsed/Refractory Diffuse Large B-Cell Lymphoma with Deficit of Vitamin D Levels Using R-GDP Plus Lenalidomide (R2-GDP): Results from the R2-GDP-GOTEL Trial
oai:idus.us.es:11441/1085782024-02-14T09:26:18Zcom_11441_10989com_11441_10983com_11441_10690com_11441_10999col_11441_10990col_11441_11000
00925njm 22002777a 4500
dc
2019-10-02
The placental stem cells have called the focus of attention for their therapeutic potential to treat
diferent diseases, including cancer. There is plenty evidence about the antiproliferative, antiangiogenic
and proapoptotic properties of the amniotic membrane. Liver cancer is the ffth cause of cancer in the
world, with a poor prognosis and survival. Alternative treatments to radio- or chemotherapy have
been searched. In this work we aimed to study the antiproliferative properties of the human amniotic
membrane conditioned medium (AM-CM) in hepatocarcinoma cells. In addition, we have analyzed
the regulation of pro and antiOncomiRs expression involved in hepatocarcinoma physiology. We have
determined by 3H-thymidine incorporation assay that AM-CM inhibits DNA synthesis in HepG2 cells
after 72h of treatment. AM-CM pure or diluted at 50% and 25% also diminished HepG2 and HuH-7 cells
viability and cell number. Furthermore, AM-CM induced cell cycle arrest in G2/M. When proliferation
mechanisms were analyzed we found that AM-CM reduced the expression of both Cyclin D1 mRNA
and protein. Nuclear expression of Ki-67 was also reduced. We observed that this CM was able to
promote the expression of p53 and p21 mRNA and proteins, leading to cell growth arrest. Moreover,
AM-CM induced an increase in nuclear p21 localization, observed by immunofuorescence. As p53
levels were increased, Mdm-2 expression was downregulated. Interestingly, HepG2 and HuH-7 cells
treatment with AM-CM during 24 and 72h produced an upregulation of antiOncomiRs 15a and 210, and
a downregulation of proOncomiRs 206 and 145. We provide new evidence about the promising novel
applications of human amniotic membrane in liver cancer.
Riedel, R., Pérez Pérez, A., Carmona Fernández, A., Guadix, P., Sánchez Margalet, V. y Maymó, J.L. (2019). Human amniotic membrane conditioned medium inhibits proliferation and modulates related microRNAs expression in hepatocarcinoma cell. Scientific Reports, 9 (14193)
2045-2322
https://hdl.handle.net/11441/108578
10.1038/s41598-019-50648-5
MicroRNAs
AM-CM
Hepatocarcinoma cells
Human amniotic membrane conditioned medium inhibits proliferation and modulates related microRNAs expression in hepatocarcinoma cell
oai:idus.us.es:11441/1474932024-02-14T08:58:08Zcom_11441_10989com_11441_10983com_11441_10690com_11441_10878com_11441_10802com_11441_10913col_11441_10990col_11441_10879col_11441_10914
00925njm 22002777a 4500
dc
2022-07-14
Gene therapy is a technique that is currently under expansion and development. Recent advances in genetic medicine have paved the way for a broader range of therapies and laid the groundwork for next-generation technologies. A terminally substituted difluorene-diester Schiff Base calix[4]arene has been studied in this work as possible nanovector to be used in gene therapy. Changes to luminescent behavior of the calixarene macrocycle are reported in the presence of ct-DNA. The calixarene macrocycle interacts with calf thymus DNA (ct-DNA), generating changes in its conformation. Partial double-strand denaturation is induced at low concentrations of the calixarene, resulting in compaction of the ct-DNA. However, interaction between calixarene molecules themselves takes place at high calixarene concentrations, favoring the decompaction of the polynucleotide. Based on cytotoxicity studies, the calixarene macrocycle investigated has the potential to be used as a nanovehicle and improve the therapeutic efficacy of pharmacological agents against tumors.
Lebrón Romero, J.A., Moyá Morán, M.L., López López, M., Deasy, M., Muñoz Wic, A., García Calderón, C.B.,...,López-Cornejo, M.d.P. (2022). Fluorescent calixarene-schiff as a nanovehicle with biomedical purposes. Chemosensors, 10 (7), 281. https://doi.org/10.3390/chemosensors10070281.
2227-9040
https://hdl.handle.net/11441/147493
10.3390/chemosensors10070281
Calixarenes
Ct-DNA
Denaturation
Compaction
Gene Therapy
Nanocarriers
Fluorescent calixarene-schiff as a nanovehicle with biomedical purposes
oai:idus.us.es:11441/1507242024-02-14T08:57:27Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2020
The current management of alperujo as the main solid by-product from the two-phase olive oil extraction system has led to the appearance of a new liquid effluent that until now was treated together with the alperujo itself. The composition and antioxidant properties of its bioactive components at different depths of the pond were studied using colorimetric and HPLC with UV and MS detectors, DPPH, reducing power and rancimat. The concentration of suspended solids varied between 1.71 and 8.49 g/L, total fat was between 0.74 and 1.47 g/L, and total phenols were found between 3.74 and 4.11 g/L, which included hydroxytyrosol, 3,4-dihydroxyphenylglycol and tyrosol as the main phenols. Two types of extracts were obtained through two industrial systems with ethyl acetate and by chromotography, with an average content in total sugars of 2.1% and 3.16%, total phenols of 17.9% and 28.6% and hydroxytyrosol of 51.5 and 79.0 mg/g of extract, respectively. The activity presented by the chromatographic extract was higher in terms of free radical sequestering capacity, reducing power and the inhibition of lipid oxidation. Obtaining bioactive extracts would improve the formulation of food with natural components and at the same time would be the first step in a biorefinery to improve the management of the new effluent.
Fernández Prior, Á., Pérez Fatuarte, J.C., Bermúdez Oria, A., Viera Alcaide, I., Fernández Bolaños, J. y Rodríguez Gutiérrez, G. (2020). New Liquid Source of Antioxidant Phenolic Compounds in the Olive Oil Industry: Alperujo Water. Foods, 9 (7), 1-14. https://doi.org/10.3390/foods9070962.
2304-8158
https://hdl.handle.net/11441/150724
10.3390/foods9070962
Olive oil
Phenolic extracts
Alperujo
Hydroxytyrosol
3,4-dihydroxyphenylglycol
Antioxidant
Functional foods
New Liquid Source of Antioxidant Phenolic Compounds in the Olive Oil Industry: Alperujo Water
oai:idus.us.es:11441/1563382024-03-15T16:17:38Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2021
Background: Heart failure (HF) is a major and growing medical and economic problem, with high prevalence and
incidence rates worldwide. Cardiac Biomarker is emerging as a novel tool for improving management of patients with
HF with a reduced left ventricular ejection fraction (HFrEF).
Methods: This is a before and after interventional study, that assesses the impact of a personalized follow-up proce‑
dure for HF on patient’s outcomes and care associated cost, based on a clinical model of risk stratifcation and person‑
alized management according to that risk. A total of 192 patients were enrolled and studied before the intervention
and again after the intervention. The primary objective was the rate of readmissions, due to a HF. Secondary outcome
compared the rate of ED visits and quality of life improvement assessed by the number of patients who had reduced
NYHA score. A cost-analysis was also performed on these data.
Results: Admission rates signifcantly decreased by 19.8% after the intervention (from 30.2 to 10.4), the total hospital
admissions were reduced by 32 (from 78 to 46) and the total length of stay was reduced by 7 days (from 15 to 9 days).
The rate of ED visits was reduced by 44% (from 64 to 20). Thirty-one percent of patients had an improved functional
class score after the intervention, whereas only 7.8% got worse. The overall cost saving associated with the interven‑
tion was € 72,769 per patient (from € 201,189 to € 128,420) and €139,717.65 for the whole group over 1 year.
Conclusions: A personalized follow-up of HF patients led to important outcome benefts and resulted in cost sav‑
ings, mainly due to the reduction of patient hospitalization readmissions and a signifcant reduction of care-associ‑
ated costs, suggesting that greater attention should be given to this high-risk cohort to minimize the risk of hospitali‑
zation readmissions.
León Justel, A., Morgado García-Polavieja, J.I., Álvarez-Ríos, A.I., Caro Fernández, F.J., Pájaro Merino, P.A., Gálvez Ríos, E.,...,Díaz Fernández, J.F. (2021). Biomarkers-based personalized follow-up in chronic heart failure improves patient's outcomes and reduces care associate cost. Health And Quality of Life Outcomes, 19 (1), 142. https://doi.org/10.1186/s12955-021-01779-9.
1477-7525
https://hdl.handle.net/11441/156338
10.1186/s12955-021-01779-9
Heart failure
Personalized medicine
Patient value
Patient outcomes
Budget impact
Biomarkers
Biomarkers-based personalized follow-up in chronic heart failure improves patient's outcomes and reduces care associate cost
oai:idus.us.es:11441/1472912024-02-14T20:04:53Zcom_11441_10989com_11441_10983com_11441_10690com_11441_10999col_11441_10990col_11441_11000
00925njm 22002777a 4500
dc
2023
López Enriquez, S., Porras González, C., Moreno-Luna, R., Ebert, C.S.J., Alba Jiménez, G., Santa-María Pérez, C.,...,Sánchez Gómez, S. (2023). Tissue-specific activated regulatory lymphocytes immunophenotype in chronic rhinosinusitis with nasal polyps. ARCHIVOS DE BRONCONEUMOLOGIA, 59 (5), 337-340. https://doi.org/10.1016/j.arbres.2022.12.013.
0300-2896
https://hdl.handle.net/11441/147291
10.1016/j.arbres.2022.12.013
Tissue-specific activated regulatory lymphocytes immunophenotype in chronic rhinosinusitis with nasal polyps
oai:idus.us.es:11441/1469302024-02-13T19:57:47Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2023-01-24
The Mediterranean diet (MD) has beneficial effects on human health, which is evidenced
by the observation of lower incidence rates of chronic diseases in Mediterranean countries. The MD
dietary pattern is rich in antioxidants, such as melatonin, which is a hormone produced mainly by the
pineal gland and controls several circadian rhythms. Additionally, melatonin is found in foods, such
as fruit and vegetables. The purpose of this systematic review was to assess the melatonin content in
Mediterranean foods and to evaluate the influence of the MD on melatonin levels in both humans
and model organisms. A comprehensive search was conducted in four databases (PubMed, Scopus,
Cochrane Library and Web of Science) and data were extracted. A total of 31 records were chosen.
MD-related foods, such as tomatoes, olive oil, red wine, beer, nuts, and vegetables, showed high
melatonin contents. The consumption of specific MD foods increases melatonin levels and improves
the antioxidant status in plasma.
Grao Cruces, E., Calvo Gutiérrez, J.R., Maldonado y Aibar, M.D., Millán-Linares, M.d.C. y Montserrat de la Paz, S. (2023). Mediterranean Diet and Melatonin: A Systematic Review. ANTIOXIDANTS, 12 (2), 264. https://doi.org/10.3390/antiox12020264.
122076-3921
https://hdl.handle.net/11441/146930
10.3390/antiox12020264
Antioxidant
Beer
Circadian rhythm
Nuts
Tomato
Olive oil
Sleep
Wine
Mediterranean Diet and Melatonin: A Systematic Review
oai:idus.us.es:11441/1394132022-11-14T17:48:23Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2021-11-05
Atopic dermatitis (AD) is the most frequent chronic inflammatory skin disease, and its incidence has been rapidly increasing in developed countries in the last years. AD presents a high degree of heterogeneity due to biases and confounding factors such as age range, sex, or ethnicity. For those reasons, the search for new biomarkers is crucial. At the same time, obesity, which is a global health problem, has also increased over the years. It has been associated with many pathophysiological states, including skin diseases such as AD, mostly in childhood. Obesity promotes a low grade inflammation driven by many different cytokines and adipokines, including leptin, which has a key role in many other diseases due to its pleiotropic effects. Leptin also has a role in both skin and allergic diseases very related to AD. Thus, this adipokine could have an important role in the pathogenesis of AD, especially in its chronicity. Despite the limited literature available, there is some evidence that leads us to consider leptin as an important adipokine in this skin disease. For this reason, here we have reviewed the role of leptin in the pathophysiology of AD.
Jiménez Cortegana, C., Ortiz García, G., Serrano, A., Moreno Ramírez, D. y Sánchez Margalet, V. (2021). Possible Role of Leptin in Atopic Dermatitis: A Literature Review. Biomolecules, 11 (11), 1642. https://doi.org/10.3390/biom11111642.
2218-273X
https://hdl.handle.net/11441/139413
10.3390/biom11111642
Atopic dermatitis
Obesity
Inflammation
Leptin
Adipokine
Immune system
Possible Role of Leptin in Atopic Dermatitis: A Literature Review
oai:idus.us.es:11441/1554372024-02-22T13:18:52Zcom_11441_10989com_11441_10983com_11441_10690com_11441_10692com_11441_10691com_11441_11190com_11441_11099com_11441_10873com_11441_10802com_11441_11366com_11441_11290col_11441_10990col_11441_10693col_11441_43346col_11441_10874col_11441_11367
00925njm 22002777a 4500
dc
2003
Este artículo reúne, primero, las percepciones heterogéneas que tuvieron estudiantes de la Universidad de Sevilla
sobre la comunicación didáctica en diez
aulas mientras se desarrollaban innovaciones curriculares, que no tuvieron un
tiempo único de implantación: el emplazamiento de las mismas se dilató a lo
largo del curso 2000-2001 en materias
anuales y cuatrimestrales. Segundo, el
profesorado utilizó registros metodológicos distintos, una mezcla de acordes
innovadores de donde brotó una rica variedad estilística
Villar Angulo, L.M., Correa-Manfredi, J., Gaytán Guía, S.P., Hervás Gómez, C., Maldonado y Aibar, M.D., Medianero-Hernández, J.M.,...,Cantillana Merchante, C. (2003). Innovación curricular, ambiente percibido y desarrollo profesional en la Universidad de Sevilla. Revista española de pedagogía, 225, 265-283.
0034-9461
https://hdl.handle.net/11441/155437
Innovación curricular, ambiente percibido y desarrollo profesional en la Universidad de Sevilla
oai:idus.us.es:11441/1480822024-02-15T07:38:11Zcom_11441_10989com_11441_10983com_11441_10690com_11441_10994com_11441_10873com_11441_10802col_11441_10990col_11441_10995col_11441_10874
00925njm 22002777a 4500
dc
2023
Some HIV controllers experience immunologic progression with CD4+ T cell decline. We aimed to identify genetic factors associated with CD4+ T cell lost in HIV controllers. A total of 561 HIV controllers were included, 442 and 119 from the International HIV controllers Study Cohort and the Swiss HIV Cohort Study, respectively. No SNP or gene was associated with the long-term non-progressor HIV spontaneous control phenotype in the individual GWAS or in the meta-analysis. However, SNPs previously associated with natural HIV control linked to HLA-B (rs2395029 [p = 0.005; OR = 1.70], rs59440261 [p = 0.003; OR = 1.78]), MICA (rs112243036 [p = 0.011; OR = 1.45]), and PSORS1C1 loci (rs3815087 [p = 0.017; OR = 1.39]) showed nominal association with this phenotype. Genetic factors associated with the long-term HIV controllers without risk of immunologic progression are those previously related to the overall HIV controller phenotype.
Real, L.M., Sáez, M.E., Corma Gómez, A., González Pérez, A., Thorball, C., Ruiz Laza, R.,...,Ruiz Mateos, E. (2023). A metagenome-wide association study of HIV disease progression in HIV controllers. iScience, 26 (7), 107214. https://doi.org/10.1016/j.isci.2023.107214.
2589-0042
https://hdl.handle.net/11441/148082
10.1016/j.isci.2023.107214
A metagenome-wide association study of HIV disease progression in HIV controllers
oai:idus.us.es:11441/228362024-02-17T16:58:42Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2002
1415-4757
http://www.scielo.br/pdf/gmb/v25n1/a02v25n1.pdf
http://hdl.handle.net/11441/22836
https://idus.us.es/xmlui/handle/11441/22836
Fragile X founder effect and distribution of CGG repeats among the mentally retarded population of Andalusia, South Spain
oai:idus.us.es:11441/1475022024-02-14T19:36:04Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11004col_11441_10990col_11441_11005
00925njm 22002777a 4500
dc
2020
The presence of the chimeric EWSR1-FLI1 oncoprotein is the main and initiating event defining Ewing sarcoma (ES). The dysregulation of epigenomic and proteomic homeostasis induced by the oncoprotein contributes to a wide variety of events involved in oncogenesis and tumor progression. Attempts at studying the effects of EWSR1-FLI1 in non-tumor cells to understand the mechanisms underlying sarcomagenesis have been unsuccessful to date, as ectopic expression of EWSR1-FLI1 blocks cell cycle progression and induces apoptosis in the tested cell lines. Therefore, it is essential to find a permissive cell type for EWSR1-FLI1 expression that allows its endogenous molecular functions to be studied. Here we have demonstrated that HeLa cell lines are permissive to EWSR1-FLI1 ectopic expression, and that our model substantially recapitulates the endogenous activity of the EWSR1-FLI1 fusion protein. This model could contribute to better understanding ES sarcomagenesis by helping to understand the molecular mechanisms induced by the EWSR1-FLI1 oncoprotein.
García-Domínguez, D.J., Hontecillas-Prieto, L., Andrés León, E., Sánchez-Molina, S., Rodríguez-Núñez, P., Morón, F.J.,...,Álava Casado, E.d. (2020). An inducible ectopic expression system of EWSR1-FLI1 as a tool for understanding Ewing sarcoma oncogénesis. PLoS ONE, 15 (6), e0234243. https://doi.org/10.1371/journal.pone.0234243.
1932-6203
https://hdl.handle.net/11441/147502
10.1371/journal.pone.0234243
Growth
Gene
Initiation
EWS-FLI1
An inducible ectopic expression system of EWSR1-FLI1 as a tool for understanding Ewing sarcoma oncogénesis
oai:idus.us.es:11441/613392024-02-12T21:41:32Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2010-05
Vasoactive intestinal peptide (VIP) is a potent anti-inflammatory neuropeptide that, by inhibiting Th1-driven responses and inducing the emergence of regulatory T cells (Treg), has been proven successful in the induction of tolerance in various experimental models of autoimmune disorders. Here, we investigate the molecular mechanisms involved in VIP-induced tolerance. VIP treatment in the presence of T-cell receptor (TCR) signaling and CD28 costimulation induced cell cycle arrest in human T cells. VIP blocked G1/S
transition and inhibited the synthesis of cyclins D3 and E and the activation of the cyclin-dependent kinases (CDKs) cdk2 and cdk4. This effect was accompanied by maintenance of threshold levels of the CDK inhibitor p27kip1 and impairment of phosphatidylinositol 3-kinase (PI3K)-Akt signaling. Inhibition of interleukin 2 (IL-2) transcription and downregulation of signaling through NFAT, AP-1, and Ras-Raf paralleled the VIPinduced cell cycle arrest. Noteworthy from a functional point of view is the fact that VIP-treated T cells show
a regulatory phenotype characterized by high expression of CD25, cytotoxic-T-lymphocyte-associated protein 4 (CTLA4), and Forkhead box protein 3 (FoxP3) and potent suppressive activities against effector T cells.
CTLA4 appears to be critically involved in the generation and suppressive activities of VIP-induced Treg.
Finally, cyclic AMP (cAMP) and protein kinase A (PKA) activation seems to mediate the VIP-induced cell cycle arrest and Treg generation.
Anderson, P. y Gonzalez-Rey, E. (2010). Vasoactive intestinal peptide induces cell cycle arrest and regulatory functions in human T cells at multiple levels. Molecular and Cellular Biology, 30 (10), 2537-2551.
02707306
http://hdl.handle.net/11441/61339
10.1128/MCB.01282-09
Vasoactive intestinal peptide induces cell cycle arrest and regulatory functions in human T cells at multiple levels
oai:idus.us.es:11441/228402024-02-13T22:14:39Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11044col_11441_10990col_11441_11045
00925njm 22002777a 4500
dc
2003
1471-2377
http://www.biomedcentral.com/content/pdf/1471-2377-3-5.pdf
http://hdl.handle.net/11441/22840
https://idus.us.es/xmlui/handle/11441/22840
Variable expression of cerebral cavernous malformation in carriers of a premature termination codon in exon 17 of the Krit1 gene
oai:idus.us.es:11441/1553452024-02-19T14:18:07Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2009
Melatonin is a molecule with antioxidative properties including direct free radical
scavenging and indirect stimulatory actions on a variety of antioxidative enzymes which further promote
its ability to reduce the toxicity of radicals and their associated reactants. Beer is an integral element of
the diet of numerous people and is rich in antioxidants. We analyzed if melatonin is present in beer and if
so, at what concentration. It further determines whether the moderate consumption of beer has an effect
on the total antioxidant status (TAS) of human serum.
Methods: We analyzed 18 brands of beer with different percentage of alcohol content in order to
determine the concentration of melatonin. Serum samples were collected from 7 healthy volunteers.
These samples were used to measure melatonin and TAS on basal conditions and after drinking beer.
Results: Showed that all the beer analyzed did indeed contain melatonin and the more they have got, the
greater was its degree of alcohol. Both melatonin and TAS in human serum increased after drinking beer.
Conclusions: Melatonin present in the beer does contribute to the total antioxidative capability of human
serum and moderate beer consumption can protect organism from overall oxidative stress.
Maldonado y Aibar, M.D., Moreno, H. y Calvo Gutiérrez, J.R. (2009). Melatonin present in beer contributes to increase the levels of melatonin and antioxidant capacity of the human serum. Clinical Nutrition, 28 (2), 188-191. https://doi.org/10.1016/j.clnu.2009.02.001.
0261-5614
https://hdl.handle.net/11441/155345
10.1016/j.clnu.2009.02.001
Beer
Melatonin
Oxidative stress
Human serum
Diet
Melatonin present in beer contributes to increase the levels of melatonin and antioxidant capacity of the human serum
oai:idus.us.es:11441/1307602024-02-17T17:03:08Zcom_11441_10813com_11441_10802com_11441_10690com_11441_10989com_11441_10983col_11441_10814col_11441_10990
00925njm 22002777a 4500
dc
2019
We have analyzed the effects of minor compounds found in the unsaponifiable fraction (UF) and in the phenolic fraction (PF) of virgin olive oil (VOO) on LPS-induced inflammatory response via visfatin modulation in human monocytes. For this purpose, monocytes were incubated with UF and PF at different concentrations and the pro-inflammatory stimulus LPS for 24 hr; squalene (SQ) and hydroxytyrosol (HTyr), the main components in UF and PF, respectively, were also used. The relative expression of both pro-inflammatory and anti-inflammatory genes, as well as other genes related to the NAD+-biosynthetic pathway was evaluated by RT-qPCR; and the secretion of some of these markers was assessed by ELISA procedures. We found that UF, SQ, PF, and HTyr prevented from LPS-induced dysfunctional gene expression and secretion via visfatin-related gene modulation in human monocytes. These findings unveil a potential beneficial role for minor compounds of VOO in the prevention of inflammatory-disorders. Practical application: In this project, potential health benefits of VOO micronutrients (unsaponifiable and phenolic compounds) were confirmed through anti-inflammatory assays. Our results reveal new interesting researching goals concerning nutrition by considering the role of bioactive VOO compounds in the prevention and progress of diseases related to inflammation.
Martín Rubio, M.E., Millán Linares, M.C., Naranjo, M.C., Toscano Sánchez, M.d.R., Abia González, M.d.R., García Muriana, F.J.,...,Montserrat de la Paz, S. (2019). Minor compounds from virgin olive oil attenuate LPS-induced inflammation via visfatin-related gene modulation on primary human monocytes. Journal of Food Biochemistry, 43 (8), e12941.
0145-8884
1745-4514
https://hdl.handle.net/11441/130760
10.1111/jfbc.12941
NAMPT
Olive oil
Phenols
Unsaponifiable
Visfatin
Minor compounds from virgin olive oil attenuate LPS-induced inflammation via visfatin-related gene modulation on primary human monocytes
oai:idus.us.es:11441/228382024-02-14T20:09:11Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2012
1746-6148
http://www.biomedcentral.com/content/pdf/1746-6148-8-84.pdf
http://hdl.handle.net/11441/22838
https://idus.us.es/xmlui/handle/11441/22838
Vaccination prepartum enhances the beneficial effects of melatonin on the immune response and reduces platelet responsiveness in sheep
oai:idus.us.es:11441/611292024-02-13T08:56:29Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2006-10-01
The ontogenesis of the pineal gland of 20 bovine embryos (Bos taurus) has been analysed from 160 days of gestation to birth by means of optical microscopy and immunohistochemical techniques. For this study, the specimens were grouped into two stage in accordance with the most relevant histological characteristics: Stage 1 (160 to 200 days of prenatal development) and Stage 2 (220 days of prenatal development to birth). At 160 days of gestation some rounded structures with a central lumen, which we refer to as glandular rosettes, begin differentiation from the epithelium of the pineal recess, experiencing an extraordinary increase in number and size at 200 days of intrauterine life. In the interior of the pineal parenchyma we observed some morphologically rounded cells with oval euchromatic nuclei and a well-differentiated nucleolus that we refer to as the pinealoblasts. We also observed other cells characterised by the presence of low cytoplasm and rounded and highly stained nuclei that we refer to as the interstitial cells. The glandular stroma is formed from the capsular, trabecular, and perivascular connective tissue as well as from the reticular network that comes from the cellular processes of the interstitial cells. The blood vessels, at 240 of gestation, show wellformed walls where the endothelial cells stand out. At 160 days of gestation we witnessed some cells with small, dense, oval nuclei, positive to the glial fibrillary acidic protein (GFAP). At this age NPY positive fibres were detected, distributed around the blood vessels and among the pinealoblasts. We conclude by clarifying that the changes detected in the morphology as well as in the number and size of glandular rosettes appear to be related to the functional activity of the pineal gland during embryonic development.
Regodón, S., Pozo Pérez, D. y Roncero, V. (2006). Histomorphogenesis and immunohistochemical study of the bovine pineal gland (Bos taurus) during prenatal development (160 days of gestation to birth). Histology and Histopathology, 21 (10), 1043-1053.
02133911
http://hdl.handle.net/11441/61129
Pineal gland
Development
Histomorphogenesis and immunohistochemical study of the bovine pineal gland (Bos taurus) during prenatal development (160 days of gestation to birth)
oai:idus.us.es:11441/598522024-02-13T09:54:45Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11004col_11441_10990col_11441_11005
00925njm 22002777a 4500
dc
2005-07-01
Thyrotropin-releasing hormone (TRH) synthesized in the hypothalamus has the capability of inducing the release of thyroid-stimulating hormone (TSH) from the anterior pituitary, which in turn stimulates the production of thyroid hormones in the thyroid gland. Immunoreactivity for TRH and TRH-like peptides has been found in some tissues outside the nervous system, including thyroid. It has been demonstrated that thyroid C-cells express authentic TRH, affecting thyroid hormone secretion by follicular cells. Therefore, C-cells could have a paracrine role in thyroid homeostasis. If this hypothesis is true, follicular cells should express TRH receptors (TRH-Rs) for the paracrine modulation carried out by C-cells. In order to elucidate whether or not C-cell TRH production could act over follicular cells modulating thyroid function, we studied TRH-Rs expression in PC C13 follicular cells from rat thyroid, by means of immunofluorescence technique and RT-PCR analysis. We also investigated the possibility that C-cells present TRH-Rs for the autocrine control of its own TRH production. Our results showed consistent expression for both receptors, TRH-R1 and TRH-R2, in 6-23 C-cells, and only for TRH-R2 in PC C13 follicular cells. Our data provide new evidence for a novel intrathyroidal regulatory pathway of thyroid hormone secretion via paracrine/autocrine TRH signaling.
Miguel Rodríguez, M.d., Fernández-Santos, J.M., Utrilla, J.C., Carrillo Vico, A., Borrero, J., Conde Amiano, E.,..., Martín Lacave, I.M.(2005). Thyrotropin-releasing hormone receptor expression in thyroid follicular cells: a new paracrine role of C-cells?. Histology and Histopathology, 20 (3), 713-718.
02133911
http://hdl.handle.net/11441/59852
Rat thyroid
Follicular cells
Thyrotropin-releasing hormone receptor expression in thyroid follicular cells: a new paracrine role of C-cells?
oai:idus.us.es:11441/228372024-02-13T22:22:11Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2012
Broca Martínez, M.T., Rubio Calvo, A.M., Guerrero Montávez, J.M. y Macher, H. (2012). Non-Invasive Prenatal Diagnosis of Multiple Endocrine Neoplasia Type 2A Using COLD-PCR Combined with HRM Genotyping Analysis from Maternal Serum. Plos One, 7 (12)
1932-6203
http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0051024&representation=PDF
http://hdl.handle.net/11441/22837
https://idus.us.es/xmlui/handle/11441/22837
Non-Invasive Prenatal Diagnosis of Multiple Endocrine Neoplasia Type 2A Using COLD-PCR Combined with HRM Genotyping Analysis from Maternal Serum
oai:idus.us.es:11441/1445062024-02-14T19:09:01Zcom_11441_10989com_11441_10983com_11441_10690com_11441_10999col_11441_10990col_11441_11000
00925njm 22002777a 4500
dc
2022-12-22
Background: Graft-cell-free DNA (cfDNA) in the circulation of liver transplant recipients
has been proposed as a noninvasive biomarker of organ rejection. The aim of this study was to detect
donor-specific cfDNA (ds-cfDNA) in the recipient’s serum after either liver damage or rejection
using a qPCR-based method. (2) Methods: We proposed a qPCR method based on the amplification
of 10 specific insertion–deletion (InDel) polymorphisms to detect donor-specific circulating DNA
diluted in the recipient cfDNA. ds-cfDNA from 67 patients was evaluated during the first month
post-transplantation. (3) Results: Graft rejection in the first month post-transplantation was reported
in 13 patients. Patients without liver complications showed a transitory increase in ds-cfDNA levels
at transplantation. Patients with rejection showed significant differences in ds-cfDNA increase over
basal levels at both the rejection time point and several days before rejection. Receiver operator
characteristic (ROC) analysis showed that ds-cfDNA levels discriminated rejection, with an AUC of
0.96. Maximizing both sensitivity and specificity, a threshold cutoff of 8.6% provided an estimated
positive and negative predictive value of 99% and 60%, respectively. (4) Conclusions: These results
suggest that ds-cfDNA may be a useful marker of graft integrity in liver transplant patients to screen
for rejection and liver damage.
García-Fernández, N., Macher, H.C., Suárez Artacho, G., Gómez Bravo, M.Á., Molinero Hueso, P., Guerrero Montávez, J.M. y Rubio Calvo, A.M. (2022). Donor-specific cell-free DNA qPCR quantification as a noninvasive accurate biomarker for early rejection detection in liver transplantation. JOURNAL OF CLINICAL MEDICINE, 12 (36). https://doi.org/10.3390/jcm12010036.
2077-0383
https://hdl.handle.net/11441/144506
10.3390/jcm12010036
Donor-specific cfDNA
Liver transplantation
Graft rejection
Noninvasive biomarker
Donor-specific cell-free DNA qPCR quantification as a noninvasive accurate biomarker for early rejection detection in liver transplantation
oai:idus.us.es:11441/1394542024-02-14T09:14:17Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2021-06-30
Non-alcoholic fatty liver disease (NAFLD), which affects about a quarter of the global
population, poses a substantial health and economic burden in all countries, yet there is no approved
pharmacotherapy to treat this entity, nor well-established strategies for its diagnosis. Its prevalence
has been rapidly driven by increased physical inactivity, in addition to excessive calorie intake
compared to energy expenditure, affecting both adults and children. The increase in the number of
cases, together with the higher morbimortality that this disease entails with respect to the general
population, makes NAFLD a serious public health problem. Closely related to the development
of this disease, there is a hormone derived from adipocytes, leptin, which is involved in energy
homeostasis and lipid metabolism. Numerous studies have verified the relationship between persistent hyperleptinemia and the development of steatosis, fibrinogenesis and liver carcinogenesis.
Therefore, further studies of the role of leptin in the NAFLD spectrum could represent an advance in
the management of this set of diseases.
Jiménez Cortegana, C., García-Galey, A., Tami, M., Pino, P.d., Carmona, I., López Enriquez, S.,...,Sánchez Margalet, V. (2021). Role of leptin in non-alcoholic fatty liver disease. Biomedicines, 9 (7), 762. https://doi.org/10.3390/biomedicines9070762.
2227-9059
https://hdl.handle.net/11441/139454
10.3390/biomedicines9070762
Fatty liver
Steatohepatitis
Obesity
Metabolic syndrome
Leptin
Role of leptin in non-alcoholic fatty liver disease
oai:idus.us.es:11441/1555602024-02-23T18:56:46Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11004com_11441_11044col_11441_10990col_11441_11005col_11441_11045
00925njm 22002777a 4500
dc
2024
Cancer is a complex disease that, despite advances in treatment and the greater understanding
of the tumor biology until today, continues to be a prevalent and lethal disease. Chemotherapy,
radiotherapy, and surgery are the conventional treatments, which have increased the survival for
cancer patients. However, the complexity of this disease together with the persistent problems due
to tumor progression and recurrence, drug resistance, or side effects of therapy make it necessary to
explore new strategies that address the challenges to obtain a positive response. One important point is
that tumor cells can interact with the microenvironment, promoting proliferation, dissemination, and
immune evasion. Therefore, immunotherapy has emerged as a novel therapy based on the modulation
of the immune system for combating cancer, as reflected in the promising results both in preclinical
studies and clinical trials obtained. In order to enhance the immune response, the combination of
immunotherapy with nanoparticles has been conducted, improving the access of immune cells to
the tumor, antigen presentation, as well as the induction of persistent immune responses. Therefore,
nanomedicine holds an enormous potential to enhance the efficacy of cancer immunotherapy. Here,
we review the most recent advances in specific molecular and cellular immunotherapy and in nano immunotherapy against cancer in the light of the latest published preclinical studies and clinical trials.
García-Domínguez, D.J., López Enriquez, S., Alba Jiménez, G., Garnacho Montero, C., Jiménez Cortegana, C., Flores-Campos, R.,...,Hontecillas-Prieto, L. (2024). Cancer nano-immunotherapy: the novel and promising weapon to fight cancer. International Journal of Molecular Sciences, 25 (2), 1195. https://doi.org/10.3390/ijms25021195.
1422-0067
https://hdl.handle.net/11441/155560
10.3390/ijms25021195
Cancer
Immunotherapy
Nanomedicine
Clinical trials
Cancer nano-immunotherapy: the novel and promising weapon to fight cancer
oai:idus.us.es:11441/1159772024-02-13T09:13:29Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11064col_11441_10990col_11441_11065
00925njm 22002777a 4500
dc
2021
The appearance on the skin of herpes virus lesions, concomitantly with the coronavirus disease 2019 (COVID-19) pandemic, leads us to suspect an underlying infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). Diagnostic reverse transcriptase polymerase chain reaction tests and immunoglobulin M (IgM) and IgG seroconversion studies have therefore been carried out. We present three cases of herpes virus infections in immunocompetent patients: one of the infections was herpes simplex 1 in a 40-year-old woman, and the other two were herpes varicella-zoster infections in a 62-year-old man and a 25-year-old woman. The patients were in the care of the southern health district of Seville of the SAS (Andalusian Health Service) during the Spanish state of alarm over the COVID-19 pandemic. The SARS-CoV-2 infection was confirmed in only one of the three cases. In this study, we briefly review the etiopathogenic role of the COVID-19 pandemic situation, whereby immunodeficiencies are generated that favour the appearance of other viral infections, such as herpes virus infections.
Maldonado y Aibar, M.D., Romero Aibar, J. y Pérez San Gregorio, M.d.l.Á. (2021). COVID-19 pandemic as a risk factor for the reactivation of herpes viruses. Epidemiology and Infection, 149, article e145.
0950-2688
1469-4409
https://hdl.handle.net/11441/115977
10.1017/S0950268821001333
COVID-19
herpes infections
immune system
psychoneuroimmunology
SARS-CoV-2
COVID-19 pandemic as a risk factor for the reactivation of herpes viruses
oai:idus.us.es:11441/1562202024-03-13T16:34:40Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2024
Amyotrophic lateral sclerosis (ALS) is a rare neuromuscular disease characterized by severe muscle weakness
mainly due to degeneration and death of motor neurons. A peculiarity of the neurodegenerative processes is the
variable susceptibility among distinct neuronal populations, exemplified by the contrasting resilience of motor
neurons innervating the ocular motor system and the more vulnerable facial and hypoglossal motor neurons. The
crucial role of vascular endothelial growth factor (VEGF) as a neuroprotective factor in the nervous system is wellestablished since a deficit of VEGF has been related to motoneuronal degeneration. In this study, we investigated
the survival of ocular, facial, and hypoglossal motor neurons utilizing the murine SOD1G93A ALS model at various
stages of the disease. Our primary objective was to determine whether the survival of the different brainstem
motor neurons was linked to disparate VEGF expression levels in resilient and susceptible motor neurons
throughout neurodegeneration. Our findings revealed a selective loss of motor neurons exclusively within the
vulnerable nuclei. Furthermore, a significantly higher level of VEGF was detected in the more resistant motor
neurons, the extraocular ones. We also examined whether TDP-43 dynamics in the brainstem motor neuron of
SOD mice was altered. Our data suggests that the increased VEGF levels observed in extraocular motor neurons
may potentially underlie their resistance during the neurodegenerative processes in ALS in a TDP-43-independent
manner. Our work might help to better understand the underlying mechanisms of selective vulnerability of motor
neurons in ALS.
Silva-Hucha, S., Fern andez de Sevilla, E.M., Humphreys, .M., Benson, F.E., Franco, J.M., Pozo Pérez, D.,...,Morcuende, S. (2024). VEGF expression disparities in brainstem motor neurons of the SOD1G93A ALS model: Correlations with neuronal vulnerability. Neurotherapeutics. https://doi.org/10.1016/j.neurot.2024.e00340.
1878-7479
https://hdl.handle.net/11441/156220
10.1016/j.neurot.2024.e00340
ALS
SOD1G93A
Brainstem motor neurons
VEGF
TDP-43
Extraocular system
VEGF expression disparities in brainstem motor neurons of the SOD1GP3A ALS model: Correlations with neuronal vulnerability
oai:idus.us.es:11441/246782024-02-14T19:15:48Zcom_11441_11100com_11441_11099com_11441_10690com_11441_10989com_11441_10983com_11441_11115com_11441_10692com_11441_10691com_11441_11195com_11441_11321com_11441_11290com_11441_11230com_11441_10777com_11441_2781com_11441_2379com_11441_137148col_11441_11102col_11441_10990col_11441_11116col_11441_10693col_11441_11196col_11441_11322col_11441_11231col_11441_10778col_11441_2799
00925njm 22002777a 4500
dc
2001
Morales Lozano, J.A., Villar Angulo, L.M., Barrera, J.M., Betancourt Serna, F., Caro González, F.J., Casanueva Rocha, C.,...,Camúñez Ruiz, J.A. (2001). Metaevaluación: un inquietante modelo. Revista de Enseñanza Universitaria, 17, 43-76.
1132-8983
http://institucional.us.es/revistas/universitaria/17/art%203.pdf
http://hdl.handle.net/11441/24678
https://idus.us.es/xmlui/handle/11441/24678
Metaevaluación: un inquietante modelo
oai:idus.us.es:11441/1375912022-10-06T13:41:03Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2021-03-01
A new coronavirus respiratory disease (COVID-19) caused by the SARS-CoV-2 virus, surprised the entire world, producing social, economic, and health problems. The COVID-19 triggers a lung infection with a multiple proinflammatory cytokine storm in severe patients. Without effective and safe treatments, COVID-19 has killed thousands of people, becoming a pandemic. Stem cells have been suggested as a therapy for lung-related diseases. In particular, mesenchymal stem cells (MSCs) have been successfully tested in some clinical trials in patients with COVID-19. The encouraging results positioned MSCs as a possible cell therapy for COVID-19. The amniotic membrane from the human placenta at term is a valuable stem cell source, including human amniotic epithelial cells (hAECs) and human mesenchymal stromal cells (hAMSCs). Interestingly, amnion cells have immunoregulatory, regenerative, and anti-inflammatory properties. Moreover, hAECs and hAMSCs have been used both in preclinical studies and in clinical trials against respiratory diseases. They have reduced the inflammatory response and restored the pulmonary tissue architecture in lung injury in vivo models. Here, we review the existing data about the stem cells use for COVID-19 treatment, including the ongoing clinical trials. We also consider the non-cellular therapies that are being applied. Finally, we discuss the human amniotic membrane cells use in patients who suffer from immune/inflammatory lung diseases and hypothesize their possible use as a successful treatment against COVID-19.
Riedel, R.N., Pérez Pérez, A., Sánchez Margalet, V., Varone, C.L. y Maymó, J.L. (2021). Stem cells and COVID-19: are the human amniotic cells a new hope for therapies against the SARS-CoV-2 virus?. Stem cell research & therapy, 12 (1). https://doi.org/10.1186/s13287-021-02216-w.
1757-6512(electrónico)
https://hdl.handle.net/11441/137591
10.1186/s13287-021-02216-w
COVID-19
SARS-CoV-2
Stem cells
Stem cell therapy
Mesenchymal stem cells
Amnion
Human amniotic epithelial cells
Human amniotic mesenchymal stromal cells
Stem cells and COVID-19: are the human amniotic cells a new hope for therapies against the SARS-CoV-2 virus?
oai:idus.us.es:11441/1540372024-01-25T17:29:15Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2021
Local respiratory allergy (LRA) is defined by the negativity of atopy tests, a clinical history suggestive of airway allergy and a positive response to the nasal and/or bronchial allergen challenge. The clinical spectrum of LRA is comprised of three conditions: local allergic rhinitis (LAR) and local allergic asthma in non-atopic patients, and dual allergic rhinitis (coexistence of allergic rhinitis and LAR) in atopic individuals. LRA is an independent disease phenotype not progressing to atopy over time, but naturally evolving to the clinical worsening and the onset of comorbidities. Published data suggests that LRA is mediated through the mucosal synthesis of allergen-specific (s)IgE, which binds to FcϵRI on resident mast cells, and in >50% of cases traffics to the blood stream to sensitize circulating basophils. To date, 4 clinical trials have demonstrated the capacity of allergen immunotherapy (AIT) to decrease nasal, conjunctival and bronchial symptoms, to improve quality of life, to increase the threshold dose of allergen eliciting respiratory symptoms, and to induce serum sIgG4 in LRA individuals. Collectively, these data indicate that local allergy is a relevant disease mechanisms in both atopic and non-atopic patients with airway diseases.
Testera-Montes, A., Salas, M., Palomares, F., Ariza, A., Torres, M.J., Rondón, C. y Eguiluz-Gracia, I. (2021). Local Respiratory Allergy: From Rhinitis Phenotype to Disease Spectrum. frontiers in Immunology, 12, 691964. https://doi.org/10.3389/fimmu.2021.691964.
1664-3224
https://hdl.handle.net/11441/154037
10.3389/fimmu.2021.691964
Respiratory Allergy
Rhinitis Phenotype
Disease Spectrum
Local Respiratory Allergy: From Rhinitis Phenotype to Disease Spectrum
oai:idus.us.es:11441/955202024-02-13T20:24:41Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2017
Age-related macular degeneration (AMD) is a medical condition of central loss vision and blindness. Numerous studies have revealed that changes on certain dietary fatty acids (FAs) could have useful for AMD management. This review summarizes the effects of dietary omega-3 long-chain PUFAs, MUFAs, and SFAs, and lipoproteins on AMD. Findings are consistent with the beneficial role of dietary omega-3 long-chain PUFAs, while the effects of dietary MUFAs and SFAs appeared to be ambiguous with respect to the possible protection from MUFAs and to the possible adverse impact from SFAs on AMD. Some of the pathological mechanisms associated with lipoproteins on AMD share those observed previously in cardiovascular diseases.
It was also noticed that the effects of FAs in the diet and lipoprotein on AMD could be modulated by genetic variants. From a population health perspective, the findings of this review are in favour of omega-3 long-chain FAs recommendations in a preventive and therapeutic regimen to attain lower AMD occurrence and progression rates. Additional long-term and short-term nutrigenomic studies are required to clearly establish the role and the relevance of interaction of dietary FAs, lipoproteins, and genes in the genesis and progression of AMD.
Montserrat de la Paz, S., Naranjo Gutiérrez, M.d.C., Bermúdez Pulgarín, B., López Martín, S., Abia González, M.d.R. y García Muriana, F.J. (2017). Dietary fatty acids and lipoproteins on progression of age-related macular degeneration. Grasas y aceites. International Journal of Fats and Oils, 68 (2), 1-7.
0017-3495
https://hdl.handle.net/11441/95520
10.3989/gya.0830162
Age-related macular degeneration
Dietary fats
Fatty acids
Lipoproteins
Olive oil
Retina
Dietary fatty acids and lipoproteins on progression of age-related macular degeneration
oai:idus.us.es:11441/787412024-02-17T16:33:52Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2013-11-28
The STAR family of proteins links signaling pathways to various aspects of post-transcriptional regulation and processing of RNAs. Sam68 belongs to this class of heteronuclear ribonucleoprotein particle K (hnRNP K) homology (KH) single domain-containing family of RNA-binding proteins that also contains some domains predicted to bind critical components in signal transduction pathways. In response to phosphorylation and other post-transcriptional modifications, Sam68 has been shown to have the ability to link signal transduction pathways to downstream effects regulating RNA metabolism, including transcription, alternative splicing or RNA transport. In addition to its function as a docking protein in some signaling pathways, this prototypic STAR protein has been identified to have a nuclear localization and to take part in the formation of both nuclear and cytosolic multi-molecular complexes such as Sam68 nuclear bodies and stress granules. Coupling with other proteins and RNA targets, Sam68 may play a role in the regulation of differential expression and mRNA processing and translation according to internal and external signals, thus mediating important physiological functions, such as cell death, proliferation or cell differentiation.
Sánchez-Jiménez, F. y Sánchez Margalet, V. (2013). Role of Sam68 in post-transcriptional gene regulation. International Journal of Molecular Sciences, 14 (2), 23402-23419.
1422-0067
https://hdl.handle.net/11441/78741
10.3390/ijms141223402
SAM68
RNA-binding protein
Post-transcriptional regulation
Role of Sam68 in post-transcriptional gene regulation
oai:idus.us.es:11441/1438402024-02-13T08:57:16Zcom_11441_10813com_11441_10802com_11441_10690com_11441_10989com_11441_10983col_11441_10814col_11441_10990
00925njm 22002777a 4500
dc
2023
Hemp is the term commonly used to refer to the variety of Cannabis sativa L. cultivated for industrial purposes. The seeds have gained interest in recent years as functional foods due to their nutritional composition and high content of protein and bioactive compounds. In this study, ten hemp protein hydrolysates (HPHs) were obtained by enzymatic hydrolysis with Alcalase and Flavourzyme from hemp protein isolate (HPI) and their antioxidant properties (DPPH radical scavenging activity, beta-carotene activity and ferric ion reducing antioxidant power (FRAP)) were evaluated. Shorter peptide sequences, mainly obtained with Flavourzyme, were found to react with free radicals more easily. The peptidome of all the hydrolysates was characterized, identifying, and quantifying the peptides. Furthermore, 19 unique peptides were assessed by in silico tools to hypothesize those that could be responsible of the bioactivity reported for the hydrolysates. From the identified peptides, based on the molecular features and the predictions, the peptides KNAIYTPH, EERPGHF, and KNGMMAPH, among others, are proposed to be highly contributing to the antioxidant activity of the hydrolysates.
Montserrat de la Paz, S., Rivero-Pino, F., Villanueva, Á., Toscano Sánchez, M.d.R., Martín Rubio, M.E., Millán, F. y Millán-Linares, M.d.C. (2023). Nutritional composition, ultrastructural characterization, and peptidome profile of antioxidant hemp protein hydrolysates. Food Bioscience, 53, 102561. https://doi.org/10.1016/j.fbio.2023.102561.
2212-4292
2212-4306
https://hdl.handle.net/11441/143840
10.1016/j.fbio.2023.102561
Bioactive peptides
Bioeconomy
Cannabis sativa
Peptidomics
Proteomic profile
Nutritional composition, ultrastructural characterization, and peptidome profile of antioxidant hemp protein hydrolysates
oai:idus.us.es:11441/1469992023-06-07T09:39:52Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2020
Very rigid supports are useful for enzyme immobilization to design continuous flow reactors and/or to work in non-conventional media. Among them, epoxy-methacrylic supports are easily functionalized with glyoxyl groups, which makes them ideal candidates for enzyme stabilization via multipoint covalent immobilization. However, these supports present highly hydrophobic surfaces, which might promote very undesirable effects on enzyme activity and/or stability. The hydrophilization of the support surface after multipoint enzyme immobilization is proposed here as an alternative to reduce these undesirable effects. The remaining aldehyde groups on the support are modified with aminated hydrophilic small molecules (glycine, lysine or aspartic acid) in the presence of 2-picoline borane. The penicillin G acylase from Escherichia coli (PGA) and alcohol dehydrogenase from Thermus thermophilus HB27 (ADH2) were immobilized on glyoxyl-functionalized agarose, Relizyme and Relisorb. Despite the similar density of aldehyde groups displayed by functionalized supports, their stabilization effects on immobilized enzymes were quite different: up to 300-fold lower by hydrophobic supports than by highly hydrophilic glyoxyl-agarose. A dramatic increase in the protein stabilities was shown when a hydrophilization treatment of the hydrophobic support surface was done. The PGA immobilized on the glyoxyl-Relisorb hydrophilized with aspartic acid becomes 280-fold more stable than without any treatment, and it is even more stable than the PGA immobilized on the glyoxyl agarose.
Orrego, A.H., Romero Fernández, M., Millán Linares, M.C., Pedroche, J., Guisán, J.M. y Rocha Martín, J. (2020). High Stabilization of Enzymes Immobilized on Rigid Hydrophobic Glyoxyl-Supports: Generation of Hydrophilic Environments on Support Surfaces. Catalysts, 10 (6), 1-10. https://doi.org/10.3390/catal10060676.
2073-4344
https://hdl.handle.net/11441/146999
10.3390/catal10060676
Protein stabilization
Protein immobilization
2-picoline borane
Methacrylic support
Microenvironment
Biocatalysis
Support hydrophilization
High Stabilization of Enzymes Immobilized on Rigid Hydrophobic Glyoxyl-Supports: Generation of Hydrophilic Environments on Support Surfaces
oai:idus.us.es:11441/1508102024-02-14T20:15:00Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2020
The increasing world population has led to the need to search for new protein sources, such as insects, the harvesting of which can be economical and environmentally sustainable. This study explores the biological activities (angiotensin-converting enzyme (ACE) inhibition, antioxidant capacity, and dipeptidyl peptidase IV (DPP-IV) inhibition) of Tenebrio molitor hydrolysates produced by a set of food-grade pro-
teases, namely subtilisin, trypsin, ficin and flavourzyme, and the degree of hydrolysis (DH), ranging from 5% to 20%. Trypsin hydrolysates exhibited the highest ACE inhibitory activity at a DH of 10% (IC50 0.27 mg mL−1) in the experimental series, which was attributed to the release of short peptides containing Arg or Lys residues in the C terminus, and described as the ACE-inhibition feature. The levels of in vitro anti-
oxidant activities were comparable to those reported for insect species. Subtilisin and trypsin hydrolysates at a DH of 10% displayed optimal DPPH scavenging and ferric reducing activities, which was attributed to the presence of 5–10-residue active peptides, as reported in the literature. Iron chelating activity was significantly favoured by increasing the DH, attaining a minimal IC50 of 0.8 mg mL−1 at a DH of 20% regardless of the enzymatic treatment. Similarly, in vitro antidiabetic activity was significantly improved by extensive hydrolysis, and, more specifically, the presence of di- and tripeptides. In this regard, the combined treatment of subtilisin–flavourzyme at a DH of 20% showed maximal DPP-IV inhibition (IC50 2.62 mg mL−1). To our knowledge, this is the first study evaluating the DPP-IV activity of Tenebrio molitor hydrolysates obtained from these commercial proteases. We conclude that Tenebrio molitor hydrolysates produced with food-grade proteases are a valuable source of active peptides that can be used as functional ingredients in food and nutraceutical preparations.
Rivero Pino, F., Pérez Gálvez, R., Espejo Carpio, F.J. y Guadix, E.M. (2020). Evaluation of Tenebrio molitor protein as a source of peptides for modulating physiological processes. Food & Function, 11 (5), 4376-4386. https://doi.org/10.1039/d0fo00734j.
2042-6496
2042-650X
https://hdl.handle.net/11441/150810
10.1039/d0fo00734j
Proteins
Angiotensin-converting enzyme (ACE)
Antioxidant capacity
Dipeptidyl peptidase IV (DPP-IV)
Tenebrio molitor
Evaluation of Tenebrio molitor protein as a source of peptides for modulating physiological processes
oai:idus.us.es:11441/1444322024-02-13T20:12:26Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11054col_11441_10990col_11441_11055
00925njm 22002777a 4500
dc
2023
Background: New challenges in our society are appearing following the emergence of antibiotic-resistant mi-
croorganisms and the concerns about the use of antibiotics. Furthermore, food spoilage due to microorganisms
leads to food waste of around 1.3 billion tons each year. Bioactive peptides obtained from food proteins, released
by enzymatic hydrolysis or fermentation, can be employed as a natural alternative to antibiotics and as pre-
servative agents, due to their ability to inhibit the growth of several microorganisms. Plant protein use in the
food industry is increasing over animal protein, due to the environmental and animal welfare concerns of the
population. Considering the concerns about the use antibiotics, their exploitation as a source of antimicrobial
peptides is gaining interest in recent years.
Scope and approach: This review aims to summarize the published literature on protein hydrolysates as a source of
antimicrobial peptides, obtained by enzymatic hydrolysis or fermentation of plants, their mechanisms, and the
use in the food industry by producing fortified products or edible coatings.
Key findings and conclusions: Antibacterial and antifungal plant peptides obtained by enzymatic hydrolysis are
studied to a larger extent that antiviral peptides but further research concerning identification and applicability
must be done. The results published show promising uses in the food industry in many areas such as fortification
of foods or active packaging with dual bioactivity, related to the antimicrobial activity – avoiding food spoilage
and antimicrobial resistance.
Rivero Pino, F., León León, M.J., Millán-Linares, M.d.C. y Montserrat de la Paz, S. (2023). Antimicrobial plant-derived peptides obtained by enzymatic hydrolysis and fermentation as components to improve current food systems. Trends in Food Science and Technology, 135, 32-42. https://doi.org/10.1016/j.tifs.2023.03.005.
0924-2244
https://hdl.handle.net/11441/144432
10.1016/j.tifs.2023.03.005
Antibacterial
Antifungal
Antivirus
Bioactive peptides
Protein hydrolysate
Antimicrobial plant-derived peptides obtained by enzymatic hydrolysis and fermentation as components to improve current food systems
oai:idus.us.es:11441/1374592024-02-14T11:09:50Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11049com_11441_11044col_11441_10990col_11441_11050col_11441_11045
00925njm 22002777a 4500
dc
2021-07-13
Identification of the different elements intervening at the tumor microenvironment seems key to explain clinical evolution in several tumor types. In this study, a set of immune biomarkers (myeloid derived suppressor cells, regulatory T cells, and OX40 + and PD-1 + T lymphocytes counts) in peripheral blood of patients diagnosed with advanced breast cancer were analyzed along of first line antineoplastic therapy. Subsequently, a comparison between groups with clinical benefit versus progression of disease and with a healthy women cohort was executed. Results reflected that patients showed higher basal levels of myeloid derived suppressor cells (35.43, IR = 180.73 vs 17.53, IR = 16.96 cells/μl; p = 0.001) and regulatory T cells (32.05, IR = 29.84 vs 22.61, IR = 13.57 cells/μl; p = 0.001) in comparison with healthy women. Furthermore, an increase in the number of activated T lymphocytes (expressing OX40), a decrease of immune inhibitory cells (MDSCs and Tregs) and inhibited T lymphocytes (expressing PD-1) were observed along the treatment in patients with clinical benefit (p ≤ 0.001). The opposite trend was observed in the case of disease progression. These findings suggest that some critical immune elements can be easily detected and measured in peripheral blood, which open a new opportunity for translational research, as they seem to be correlated with clinical evolution, at least in ABC.
Palazón Carrión, N., Jiménez Cortegana, C., Sánchez León, M.L., Nieto García, M.A., Sánchez Margalet, V. y Cruz Merino, L.d.l. (2021). Circulating immune biomarkers in peripheral blood correlate with clinical outcomes in advanced breast cancer. Scientific Reports, 11 (1), 17639. https://doi.org/10.1038/s41598-021-93838-w.
2045-2322
https://hdl.handle.net/11441/137459
10.1038/s41598-021-93838-w
Circulating immune biomarkers in peripheral blood correlate with clinical outcomes in advanced breast cancer
oai:idus.us.es:11441/1505142024-02-13T08:48:57Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2020
Parkinson's disease (PD) is a frequent progressive neurodegenerative disorder. Impaired mitochondrial function is a major feature of sporadic PD. Some susceptibility or causative genes detected in PD are strongly associated with mitochondrial dysfunction including PGC1α, TFAM and GSK3β. microRNAs (miRNAs) are non-coding RNAs whose altered levels are proven in disparate PD models and human brains. Therefore, the aim of this study was to detect modulations of miRs upstream of PGC1α, TFAM and GSK3β in association with PD onset and progress. In this study, a total of 33 PD subjects and 25 healthy volunteers were recruited. Candidate miRNA (miR-376a) was selected through target prediction tools and literature survey. Chronic and acute in vitro PD models were created by MPP+-intoxicated SHSY5Y cells. The levels of miR-376a and aforementioned genes were assessed by RT-qPCR. The expression of target genes was decreased in chronic model while there were dramatically up-regulated levels of those genes in acute model of PD. miR-376a was strongly altered in both acute and chronic PD models as well as PBMCs of PD patients. Our results also showed overexpression of PGC1α, and TFAM in PBMCs is inversely correlated with down-regulation of miR-376a, suggesting that miR-376a possibly has an impact on PD pathogenesis through regulation of these genes which are involved in mitochondrial function. miR-376a expression in PD-derived PBMCs was also correlated with disease severity and may serve as a potential biomarker for PD diagnosis. This is the first study showing altered levels of miR-376a in PD models and PBMCs, suggesting the probable role of this miRNA in PD pathogenesis. The present study also proposed TFAM and PGC1α as target genes of miR-376a for the first time, through which it possibly can exert its impact on PD pathogenesis.
1582-1838
1582-4934
https://hdl.handle.net/11441/150514
10.1111/jcmm.14979
GSK3β
MiR-376a
Mitochondrial transcription factor A
Parkinson's disease
PGC1α
Modified level of miR-376a is associated with Parkinson's disease
oai:idus.us.es:11441/1463552024-02-13T08:45:32Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11044col_11441_10990col_11441_11045
00925njm 22002777a 4500
dc
2022
Purpose: New therapeutic options are needed in relapsed/refrac tory diffuse large B-cell lymphoma (R/R DLBCL). Lenalidomide based schedules can reverse rituximab refractoriness in lymphoma.
Patients and Methods: In the phase II R2-GDP trial, 78
patients unsuitable for autologous stem cell transplant received
treatment with the following schedule: lenalidomide 10 mg Days
(D)1–14, rituximab 375 mg/m2 D1, cisplatin 60 mg/m2 D1,
gemcitabine 750 mg/m2 D1 and D8, and dexamethasone 20 mg
D1–3, up to 6 cycles (induction phase), followed by lenalidomide
10 mg (or last lenalidomide dose received) D1–21 every 28 days
(maintenance phase). Primary endpoint was overall response
rate (ORR). Secondary endpoints included progression-free
survival (PFS), overall survival (OS), safety, and monitorization
of key circulating immune biomarkers (EU Clinical Trials Reg ister number: EudraCT 2014-001620-29).
Results: After a median follow-up of 37 months, ORR was
60.2% [37.1% complete responses (CR) and 23.1% partial
responses (PR)]. Median OS was 12 months (47 vs. 6 months
in CR vs. no CR); median PFS was 9 months (34 vs. 5 months in
CR vs. no CR). In the primary refractory population, ORR was
45.5% (21.2% CR and 24.3% PR). Most common grade 3–4
adverse events were thrombocytopenia (60.2%), neutropenia
(60.2%), anemia (26.9%), infections (15.3%), and febrile neutro penia (14.1%). Complete responses were associated with a sharp
decrease in circulating myeloid-derived suppressor cells and
regulatory T cells.
Conclusions: R2-GDP schedule is feasible and highly active
in R/R DLBCL, including the primary refractory population.
Immune biomarkers showed differences in responders versus
progressors.
Palazón-Carrión, N., Martín García-Sancho, A., Nogales-Fernández, E., Jiménez Cortegana, C., Carnicero-González, F., Ríos Herranz, E.,...,Cruz Merino, L.d.l. (2022). Lenalidomide plus R-GDP (R2-GDP) in Relapsed/ Refractory Diffuse Large B-Cell Lymphoma: Final Results of the R2-GDP-GOTEL Trial and Immune Biomarker Subanalysis. CLINICAL CANCER RESEARCH, 28 (17), 3658-3668. https://doi.org/10.1158/1078-0432.CCR-22-0588.
1078-0432
1557-3265
https://hdl.handle.net/11441/146355
10.1158/1078-0432.CCR-22-0588
Lenalidomide plus R-GDP (R2-GDP)
Large B-Cell Lymphoma
Lymphoma
Lenalidomide plus R-GDP (R2-GDP) in Relapsed/ Refractory Diffuse Large B-Cell Lymphoma: Final Results of the R2-GDP-GOTEL Trial and Immune Biomarker Subanalysis
oai:idus.us.es:11441/1457232024-02-14T20:08:18Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11034col_11441_10990col_11441_11035
00925njm 22002777a 4500
dc
2022-11-15
Dendritic cells (DCs) are a heterogenous population of professional antigen presenting cells whose main role is diminished in a variety of malignancies, including cancer, leading to ineffective immune responses. Those mechanisms are inhibited due to the immunosuppressive conditions found in the tumor microenvironment (TME), where myeloid-derived suppressor cells (MDSCs), a heterogeneous population of immature myeloid cells known to play a key role in tumor immunoevasion by inhibiting T-cell responses, are extremely accumulated. In addition, it has been demonstrated that MDSCs not only suppress DC functions, but also their maturation and development within the myeloid linage. Considering that an increased number of DCs as well as the improvement in their functions boost antitumor immunity, DC-based vaccines were developed two decades ago, and promising results have been obtained throughout these years. Therefore, the remodeling of the TME promoted by DC vaccination has also been explored. Here, we aim to review the effectiveness of different DCs-based vaccines in murine models and cancer patients, either alone or synergistically combined with other treatments, being especially focused on their effect on the MDSC population.
Sánchez León, M.L., Jiménez Cortegana, C., Cabrera, G., Vermeulen, E.M., Cruz Merino, L.d.l. y Sánchez Margalet, V. (2022). The effects of dendritic cell-based vaccines in the tumor microenvironment Impact on myeloid-derived suppressor cells. Frontiers in Immunology, 13, 1050484. https://doi.org/10.3389/fimmu.2022.1050484.
1664-3224
https://hdl.handle.net/11441/145723
10.3389/fimmu.2022.1050484
dendritic cells
myeloid-derived suppressor cells
vaccines
cancer immunosuppression
The effects of dendritic cell-based vaccines in the tumor microenvironment Impact on myeloid-derived suppressor cells
oai:idus.us.es:11441/863892024-02-14T09:07:18Zcom_11441_10989com_11441_10983com_11441_10690com_11441_85785com_11441_2379com_11441_137148col_11441_10990col_11441_85786
00925njm 22002777a 4500
dc
2018
Se presenta una experiencia de mejora en la asignatura Bioquímica
Humana en el Grado en Bioquímica, en concreto en dos temas teóricos
de la asignatura. En este trabajo se propone un cambio en el modelo
metodológico anteriormente basado en la clase teórica magistral.
El modelo propuesto es un aprendizaje por preguntas y problemas que
los alumnos deben ir resolviendo en grupo elaborando por si mismos
el tema a tratar. De esta forma se cambia de un modelo unidireccional
a un modelo más participativo y de trabajo autónomo, donde el
docente no tiene ya un papel tan central. Los resultados han sido muy
positivos ya que ha mejorado la participación de los alumnos. El análisis
de los cuestionarios inicial-final me ha proporcionado una información
importante de la evolución de los alumnos tras el trabajo en
clase, y también de los conocimientos iniciales de los que partían.
Rubio Calvo, A.M. (2018). Aplicación de un ciclo de mejora de la docencia en la asignatura Bioquímica Humana. Jornadas de Formación e Innovación Docente del Profesorado, 1, 695-713.
2659-5117
https://hdl.handle.net/11441/86389
10.12795/JDU.2018.i01.39
Bioquímica Humana
Grado en Bioquímica
Docencia Universitaria
Experimentación Docente Universitaria
Envejecimiento
Aplicación de un ciclo de mejora de la docencia en la asignatura Bioquímica Humana
oai:idus.us.es:11441/1495392024-02-13T22:21:10Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11044col_11441_10990col_11441_11045
00925njm 22002777a 4500
dc
2023
Background: We evaluated a new chemoimmunotherapy combination based on the anti-PD1 monoclonal antibody
pembrolizumab and the pyrimidine antimetabolite gemcitabine in HER2- advanced breast cancer (ABC) patients
previously treated in the advanced setting, in order to explore a potential synergism that could eventually obtain long
term beneft in these patients.
Methods: HER2-negative ABC patients received 21-day cycles of pembrolizumab 200 mg (day 1) and gemcitabine
(days 1 and 8). A run-in-phase (6+6 design) was planned with two dose levels (DL) of gemcitabine (1,250 mg/m2
[DL0]; 1,000 mg/m2
[DL1]) to determine the recommended phase II dose (RP2D). The primary objective was objec‑
tive response rate (ORR). Tumor infltrating lymphocytes (TILs) density and PD-L1 expression in tumors and myeloidderived suppressor cells (MDSCs) levels in peripheral blood were analyzed.
Results: Fourteen patients were treated with DL0, resulting in RP2D. Thirty-six patients were evaluated during the
frst stage of Simon’s design. Recruitment was stopped as statistical assumptions were not met. The median age was
52; 21 (58%) patients had triple-negative disease, 28 (78%) visceral involvement, and 27 (75%)≥2 metastatic locations.
Progression disease was observed in 29 patients. ORR was 15% (95% CI, 5–32). Eight patients were treated≥6 months
before progression. Fourteen patients reported grade≥3 treatment-related adverse events. Due to the small sample
size, we did not fnd any clear association between immune tumor biomarkers and treatment efcacy that could
identify a subgroup with higher probability of response or better survival. However, patients that experienced a clini‑
cal beneft showed decreased MDSCs levels in peripheral blood along the treatment.
Conclusion: Pembrolizumab 200 mg and gemcitabine 1,250 mg/m2
were considered as RP2D. The objective of
ORR was not met; however, 22% patients were on treatment for≥6 months. ABC patients that could beneft of chemoimmunotherapy strategies must be carefully selected by robust and validated biomarkers. In our heavily pre‑
treated population, TILs, PD-L1 expression and MDSCs levels could not identify a subgroup of patients for whom the
combination of gemcitabine and pembrolizumab would induce long term beneft.
Trial registration: ClinicalTrials.gov and EudraCT (NCT03025880 and 2016–001,779-54, respectively). Registration
dates: 20/01/2017 and 18/11/2016, respectively.
Cruz Merino, L.d.l., Gion, M., Cruz, J., Alonso-Romero, J.L., Quiroga, V., Moreno, F.,...,Rojo, F. (2023). Pembrolizumab in combination with gemcitabine for patients with HER2-negative advanced breast cancer: GEICAM/2015–04 (PANGEA-Breast) study. BMC CANCER, 22 (1). https://doi.org/10.1186/s12885-022-10363-3.
1471-2407
https://hdl.handle.net/11441/149539
10.1186/s12885-022-10363-3
Pembrolizumab
Chemotherapy
HER2-negative
Advanced breast cancer
TILs
PD-L1
MDSCs
Pembrolizumab in combination with gemcitabine for patients with HER2-negative advanced breast cancer: GEICAM/2015–04 (PANGEA-Breast) study
oai:idus.us.es:11441/1395232024-02-14T13:37:09Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2021-01-29
Background: The Mediterranean diet (MD) could be involved in the regulation of different
miRNAs related to metabolic syndrome (MS). Methods: We analyzed the serum level of mir-let7a-5p,
mir-21, mir-590, mir-107 and mir-192 in patients with morbid obesity and its association with the MD
and MS. Results: There is an association between the adherence to MD and higher serum levels of
mir-590. Mir-590 was lower in those patients who consumed >2 commercial pastries/week. Mir-let7a
was lower in those who consumed ≥1 sweetened drinks, in those who consumed ≥3 pieces of
fruit/day and in those who consumed less red than white meat. A lower mir-590 and mir-let7a, and a
higher mir-192 level, were found in patients who met the high-density lipoprotein cholesterol (HDL)
criterion of MS. A higher mir-192 was found in those patients who met the triglyceride criterion
of MS and in those with type 2 diabetes (T2DM). Conclusions: There is an association between
specific serum levels of miRNAs and the amount and kind of food intake related to MD. Mir-590 was
positively associated with a healthy metabolic profile and type of diet, while mir-192 was positively
associated with a worse metabolic profile. These associations could be suggestive of a possible
modulation of these miRNAs by food.
Fontalba-Romero, M.I., López Enriquez, S., Lago-Sampedro, A., García-Escobar, E., Pastori, R.L., Domínguez-Bendala, J. y García-Serrano, S. (2021). Association between the Mediterranean diet and metabolic syndrome with serum levels of miRNA in morbid obesity. Nutrients, 13 (2), 436. https://doi.org/10.3390/nu13020436.
2072-6643
https://hdl.handle.net/11441/139523
10.3390/nu13020436
Mediterranean diet
Metabolic syndrome
miRNA
Morbid obesity
Type 2 diabetes mellitus
Association between the Mediterranean diet and metabolic syndrome with serum levels of miRNA in morbid obesity
oai:idus.us.es:11441/132142017-05-10T09:06:49Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11009com_11441_2781com_11441_2379com_11441_137148col_11441_10990col_11441_11010col_11441_18590
00925njm 22002777a 4500
dc
1998
Llanos Peña, F.d. y Maldonado y Aibar, M.D. (1998). La embriología a debate: aprender discutiendo. Revista de Enseñanza Universitaria, extra 1998, 495-504.
1132-8983
http://institucional.us.es/revistas/universitaria/extra1998/art_44.pdf
http://hdl.handle.net/11441/13214
https://idus.us.es/xmlui/handle/11441/13214
La embriología a debate: aprender discutiendo
oai:idus.us.es:11441/1391822024-02-13T20:08:34Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2021-10-20
3-Poly-phosphoinositides (PIP3) regulate cell survival, division, and migration. Both PI3-kinase (phosphoinositide-3-kinase) and PTEN (phosphatase and tensin-homolog in chromosome 10) control PIP3 levels, but the mechanisms connecting PI3-kinase and PTEN are unknown. Using non-transformed cells, the activation kinetics of PTEN and of the PIP3-effector AKT were examined after the addition of growth factors. Both epidermal growth factor and serum induced the early activation of AKT and the simultaneous inactivation of PTEN (at ~5 min). This PIP3/AKT peak was followed by a general reduction in AKT activity coincident with the recovery of PTEN phosphatase activity (at ~10–15 min). Subsequent AKT peaks and troughs followed. The fluctuation in AKT activity was linked to that of PTEN; PTEN reconstitution in PTEN-null cells restored AKT fluctuations, while PTEN depletion in control cells abrogated them. The analysis of PTEN activity fluctuations after the addition of growth factors showed its inactivation at ~5 min to be simultaneous with its transient ubiquitination, which was regulated by the ubiquitin E3 ligase cCBL (casitas B-lineage lymphoma proto-oncogene). Protein-protein interaction analysis revealed cCBL to be brought into the proximity of PTEN in a PI3-kinase-dependent manner. These results reveal a mechanism for PI3-kinase/PTEN crosstalk and suggest that cCBL could be new target in strategies designed to modulate PTEN activity in cancer.
Olazábal Morán, M., Sánchez Ortega, M., Martínez Muñoz, L., Hernandez, C., Rodríguez, M.S., Mellado, M. y Carrera, A.C. (2021). Fluctuations in AKT and PTEN Activity Are Linked by the E3 Ubiquitin Ligase cCBL. Cells, 10 (11), 2803. https://doi.org/10.3390/cells10112803.
2073-4409
https://hdl.handle.net/11441/139182
10.3390/cells10112803
PTEN
CBL
E3 ubiquitin ligase
Phosphatidylinositide 3-kinase
AKT
Fluctuations in AKT and PTEN Activity Are Linked by the E3 Ubiquitin Ligase cCBL
oai:idus.us.es:11441/1553592024-02-20T12:55:40Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11049com_11441_11054col_11441_10990col_11441_11050col_11441_11055
00925njm 22002777a 4500
dc
2024
Malnutrition refers to a person’s status as under- or overnourished, and it is usually associated with an inflammation status, which can subsequently imply a different health status, as the risk of infection is increased, along with a deterioration of the immune system. Children’s immune systems are generally more susceptible to problems than adults. In the situation of malnutrition, because malnourished children’s immune systems are compromised, they are more likely to die. However, little is known about the underlying mechanism of altered immune functioning and how it relates to starvation. Nutritional interventions have been reported as cost-effective strategies to prevent or treat the development of malnourishment, considering the link between food intake and health, especially in children, and also the susceptibility of this population to diseases and how their health status during childhood might affect their long-term physiological growth. The ingestion of specific nutrients (e.g., vitamins or oligoelements) has been reported to contribute to the proper functioning of children’s immune systems. In this review, we aim to describe the basis of malnutrition and how this is linked to the immune system, considering the role of nutrients in the modulation of the immune system and the risk of infection that can occur in these situations in children, as well as to identify nutritional interventions to improve their health.
Morales, F., Montserrat de la Paz, S., León León, M.J. y Rivero-Pino, F. (2024). Effects of Malnutrition on the Immune System and Infection and the Role of Nutritional Strategies Regarding Improvements in Children’s Health Status: A Literature Review. Nutrients, 16 (1), 1-16. https://doi.org/10.3390/nu16010001.
2072-6643
https://hdl.handle.net/11441/155359
10.3390/nu16010001
Children
Immunonutrition
Immunometabolism
Infection
Nutraceuticals
Effects of Malnutrition on the Immune System and Infection and the Role of Nutritional Strategies Regarding Improvements in Children’s Health Status: A Literature Review
oai:idus.us.es:11441/1463632024-02-14T20:05:36Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11044col_11441_10990col_11441_11045
00925njm 22002777a 4500
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2022-12-08
Obesity, which is considered a pandemic due to its high prevalence, is a risk factor for many types of cancers, including lymphoma, through a variety of mechanisms by promoting an inflammatory state. Specifically, over the last few decades, obesity has been suggested not only to increase the risk of lymphoma but also to be associated with poor clinical outcomes and worse responses to different treatments for those diseases. Within the extensive range of proinflammatory mediators that adipose tissue releases, leptin has been demonstrated to be a key adipokine due to its pleotropic effects in many physiological systems and diseases. In this sense, different studies have analyzed leptin levels and leptin/leptin receptor expressions as a probable bridge between obesity and lymphomas. Since both obesity and lymphomas are prevalent pathophysiological conditions worldwide and their incidences have increased over the last few years, here we review the possible role of leptin as a promising proinflammatory mediator promoting lymphomas.
Jiménez Cortegana, C., Hontecillas Prieto, L., García-Domínguez, D.J., Zapata, F., Palazón Carrión, N., Sánchez León, M.L.,...,Sánchez Margalet, V. (2022). Obesity and Risk for Lymphoma: Possible Role of Leptin. International Journal of Molecular Sciences, 23 (24), 15530. https://doi.org/10.3390/ijms232415530.
1661-6596; 1422-0067
https://hdl.handle.net/11441/146363
10.3390/ijms232415530
lymphoma
obesity
leptin
adipokines
Obesity and Risk for Lymphoma: Possible Role of Leptin
oai:idus.us.es:11441/1308202024-02-15T07:50:04Zcom_11441_10813com_11441_10802com_11441_10690com_11441_10989com_11441_10983col_11441_10814col_11441_10990
00925njm 22002777a 4500
dc
2018
The postprandial hypertriglyceridemia is an important and largely silent disturbance involved in the genesis of numerous pathological conditions. Exaggerated and prolonged states of postprandial hypertriglyceridemia are frequently related to the ingestion of meals enriched in saturated fatty acids (SFAs). MicroRNAs are noncoding RNAs that function as gene regulators and play significant roles in both health and disease. However, differential miRNA expression between fasting and postprandial states has never been elucidated. Here, we studied the impact of a high-saturated-fat meal, mainly rich in palmitic acid, on the miRNA signature in peripheral blood mononuclear cells (PBMCs) of nine male healthy individuals in the postprandial period by using a two-step analysis: miRNA array and validation through quantitative real-time polymerase chain reaction. Compared with miRNA expression signature in PBMCs at fasting, 36 miRNAs were down-regulated and 43 miRNAs were up-regulated in PBMCs at postprandial hypertriglyceridemic peak. Six chromosomes (3, 7, 8, 12, 14 and 19) had nearly half (48.1%) of dysregulated miRNA-gene-containing regions. Down-regulated miR-300 and miR-369-3p and up-regulated miR-495-3p, miR-129-5p and miR-7-2-3p had the highest number of target genes. The differentially expressed miRNAs and their predicted target genes involved pathways in cancer, MAPK signaling pathway, endocytosis and axon guidance. Only down-regulated miRNAs notably targeted PI3K-Akt signaling pathways, whereas only up-regulated miRNAs targeted focal adhesion, Wnt signaling pathway, transcriptional misregulation in cancer and ubiquitin-mediated proteolysis. This is the first study of miRNA expression analysis of human PBMCs during postprandial hypertriglyceridemia and offers insight into new potential mechanisms by which dietary SFAs influence health or disease.
López Martín, S., Bermúdez Pulgarín, B., Montserrat de la Paz, S., Abia González, M.d.R. y García Muriana, F.J. (2018). A microRNA expression signature of the postprandial state in response to a high-saturated-fat challenge. Journal of Nutritional Biochemistry, 57, 45-55.
0955-2863
1873-4847
https://hdl.handle.net/11441/130820
10.1016/j.jnutbio.2018.03.010
A microRNA expression signature of the postprandial state in response to a high-saturated-fat challenge
oai:idus.us.es:11441/1478682024-02-14T19:26:36Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2022
Anxiety is the most prevalent psychiatric disorder worldwide, causing a substantial
economic burden due to the associated healthcare costs. Given that commercial anxiolytic treatments
may cause important side effects and have medical restrictions for prescription and high costs, the
search for new natural and safer treatments is gaining attention. Since lupin protein hydrolysate
(LPH) has been shown to be safe and exert anti-inflammatory and antioxidant effects, key risk factors
for the anxiety process and memory impairment, we evaluated in this study the potential effects of
LPH on anxiety and spatial memory in a Western diet (WD)-induced anxiety model in ApoE−/−
mice. We showed that 20.86% of the 278 identified LPH peptides have biological activity related to
anxiolytic/analgesic effects; the principal motifs found were the following: VPL, PGP, YL, and GQ.
Moreover, 14 weeks of intragastrical LPH treatment (100 mg/kg) restored the WD-induced anxiety
effects, reestablishing the anxiety levels observed in the standard diet (SD)-fed mice since they spent
less time in the anxiety zones of the elevated plus maze (EPM). Furthermore, a significant increase
in the number of head dips was recorded in LPH-treated mice, which indicates a greater exploration
capacity and less fear due to lower levels of anxiety. Interestingly, the LPH group showed similar
thigmotaxis, a well-established indicator of animal anxiety and fear, to the SD group, counteracting
the WD effect. This is the first study to show that LPH treatment has anxiolytic effects, pointing to
LPH as a potential component of future nutritional therapies in patients with anxiety.
Santos-Sánchez, G., Ponce España, E., López García, J.C., Álvarez-Sánchez, N., Álvarez López, A.I., Pedroche, J.,...,Carrillo Vico, A. (2022). A Lupin (Lupinus angustifolius) Protein Hydrolysate Exerts Anxiolytic-Like Effects in Western Diet-Fed ApoE−/− Mice. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 23 (17), 9828. https://doi.org/10.3390/ijms23179828.
1422-0067
https://hdl.handle.net/11441/147868
10.3390/ijms23179828
Lupin
Peptides
Protein hydrolysates
Anxiety
ApoE−/−
Functional foods
Peptidomics
A Lupin (Lupinus angustifolius) Protein Hydrolysate Exerts Anxiolytic-Like Effects in Western Diet-Fed ApoE−/− Mice
oai:idus.us.es:11441/876522024-02-14T20:07:18Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2016
Astrocytes actively participate in neuro-inflammatory processes associated to Alzheimer’s disease (AD), and other brain pathologies. We recently showed that an astrocyte-specific intracellular signaling pathway involving an interaction of the phosphatase calcineurin with the transcription factor FOXO3 is a major driver in AD associated pathological inflammation, suggesting a potential new druggable target for this devastating disease. We have now developed decoy molecules to interfere with calcineurin/FOXO3 interactions, and tested them in astrocytes and neuronal co-cultures exposed to amyloid-β (Aβ) toxicity.
We observed that interference of calcineurin/FOXO3 interactions exerts a protective action against A-induced neuronal death and favors the production of a set of growth factors that we hypothesize form part of a cytoprotective pathway to resolve inflammation. Furthermore, interference of the A-induced interaction of calcineurin with FOXO3 by decoy compounds significantly decreased amyloid-β protein precursor (AβPP) synthesis, reduced the AβPP amyloidogenic pathway, resulting in lower Alevels, and blocked the expression of pro-inflammatory cytokines TNFα and IL-6 in astrocytes. Collectively, these data indicate that interrupting pro-inflammatory calcineurin/FOXO3 interactions in astrocytes triggered by Aβ accumulation in brain may constitute an effective new therapeutic approach in AD. Future studies with intranasal delivery, or brain barrier
permeable decoy compounds, are warranted.
Navarro Garrido, V., Vitorica Ferrández, F.J., Fernández, A.M., Hervasa, R., Gómez Gutiérrez, P. y Vega, M. (2016). Blockade of the Interaction of Calcineurin with FOXO in Astrocytes Protects Against Amyloid-βInduced Neuronal Death. Journal of Alzheimer's Disease, 52 (4)
1875-8908
https://hdl.handle.net/11441/87652
Alzheimer’s disease
Astrocytes
Calcineurin
Decoy compounds
FOXO
Blockade of the Interaction of Calcineurin with FOXO in Astrocytes Protects Against Amyloid-βInduced Neuronal Death
oai:idus.us.es:11441/1252342021-08-31T09:49:17Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11094col_11441_10990col_11441_11095
00925njm 22002777a 4500
dc
2020-06-04
Peptides from several plant food proteins not only maintain the nutritional values of the
original protein and decrease the environmental impact of animal agriculture, but also exert biological
activities with significant health-beneficial effects. Wheat is the most important food grain source
in the world. However, negative attention on wheat-based products has arose due to the role of
gluten in celiac disease. A controlled enzymatic hydrolysis could reduce the antigenicity of wheat
gluten protein hydrolysates (WGPHs). Therefore, the aims of the present study were to evaluate
the effects of the in vitro administration of Alcalase-generated WGPHs on the immunological and
antioxidant responses of human peripheral blood mononuclear cells (PBMCs) from 39 healthy subjects.
WGPH treatment reduced cell proliferation and the production of the Type 1 T helper (Th1) and
Th17 pro-inflammatory cytokines IFN-γ and IL-17, respectively. WPGHs also improved the cellular
anti-inflammatory microenvironment, increasing Th2/Th1 and Th2/Th17 balances. Additionally,
WGPHs improved global antioxidant capacity, increased levels of the reduced form of glutathione
and reduced nitric oxide production. These findings, not previously reported, highlight the beneficial
capacity of these vegetable protein hydrolysates, which might represent an effective alternative in
functional food generation.
Cruz-Chamorro, I., Álvarez-Sánchez, N., Santos-Sánchez, G., Lardone, P.J., Bejarano, I., Guerrero, J.M. y Carrillo Vico, A. (2020). Immunomodulatory and Antioxidant Properties of Wheat Gluten Protein Hydrolysates in Human Peripheral Blood Mononuclear Cells. Nutrients, 12 (6), art.n.1673.
2072-6643 (electrónica)
https://hdl.handle.net/11441/125234
10.3390/nu12061673
Wheat gluten protein hydrolysates
Bioactive peptides
Pro-inflammatory cytokines
Oxidative stress
Antioxidant capacity
Glutathione
Peripheral blood mononuclear cells
Immunomodulatory and Antioxidant Properties of Wheat Gluten Protein Hydrolysates in Human Peripheral Blood Mononuclear Cells
oai:idus.us.es:11441/1307622024-02-12T21:39:24Zcom_11441_10813com_11441_10802com_11441_10690com_11441_10989com_11441_10983com_11441_11084col_11441_10814col_11441_10990col_11441_11085
00925njm 22002777a 4500
dc
2017
The effects of chemically synthesized metabolites (sulfate and glucuronate forms) from hydroxytyrosol (HTyr) on oxidative stress and inflammation were investigated in TNF-α-activated human endothelial cells. HTyr sulfate metabolites decreased reactive oxygen species and prevented the decrease in glutathione, glutathione peroxidase 1, and glutamate-cysteine ligase catalytic subunit and up-regulated heme oxygenase-1 levels. HTyr and all tested HTyr metabolites (HTyr sulfate > HTyr glucuronate > HTyr) suppressed the phosphorylation of nuclear factor kappa B proteins, the gene expression of intercellular and vascular adhesion molecules, E-selectin, chemokine (CC) motif ligand 2, and prostaglandin-endoperoxidase synthase 2 and the adhesion of human monocytes. In addition, HTyr sulfate metabolites suppressed plantar and ear swelling and myeloperoxidase activity in inflamed ear tissue in mice treated with carrageenan or 12-O-tetradecanoylphorbol-13-acetate. This study demonstrates the antioxidant and/or anti-inflammatory properties of HTyr metabolites in TNF-α-activated hECs and in the prevention of acute and chronic inflammation in mice.
López Martín, S., Montserrat de la Paz, S., Lucas Rodríguez, R., Bermúdez Pulgarín, B., Abia González, M.d.R., Morales, J.C. y García Muriana, F.J. (2017). Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cells. Journal of Functional Foods, 29, 238-247.
1756-4646
https://hdl.handle.net/11441/130762
10.1016/j.jff.2016.12.033
Endothelial cells
Human
Hydroxytyrosol
Inflammation
Metabolites
Mice
Effect of metabolites of hydroxytyrosol on protection against oxidative stress and inflammation in human endothelial cells
oai:idus.us.es:11441/232842024-02-17T16:58:16Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2010
0962-9351
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2846344/pdf/MI2010-568343.pdf
http://hdl.handle.net/11441/23284
https://idus.us.es/xmlui/handle/11441/23284
Role of leptin in the activation of immune cells
oai:idus.us.es:11441/173182024-02-15T07:22:14Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
1994
Goberna Ortiz, R., Sánchez Margalet, V. y Lucas, M. (1994). Pancreastatin activates protein-kinase-C by stimulating the formation of 1,2-diacylglycerol in rat hepatocytes. PLoS ONE, 303, 51-54.
1932-6203
http://www.biochemj.org/bj/303/0051/3030051.pdf
http://hdl.handle.net/11441/17318
https://idus.us.es/xmlui/handle/11441/17318
Pancreastatin activates protein-kinase-C by stimulating the formation of 1,2-diacylglycerol in rat hepatocytes
oai:idus.us.es:11441/171152024-02-17T17:31:46Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2013
Vaquero de Sedas, M.I. y Vega Palas, M.Á. (2013). Frequently cutting restriction enzymes: clearing the fog to see the ends. American Journal of Molecular Biology, 3, 59-61.
2161-6620
http://hdl.handle.net/11441/17115
https://idus.us.es/xmlui/handle/11441/17115
Frequently cutting restriction enzymes: clearing the fog to see the ends
oai:idus.us.es:11441/1553502024-02-19T14:52:21Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2012
Acute sport exercise leads to a strong stimulation of muscle tissue and a change in the organism energy demands. This study was designed to investigate the effect of oral melatonin supplementation on human physiological functions associated with acute exercise. Immune, endocrine and metabolic parameters were
measured in 16 young male football players, who were divided into two groups, an experimental group (supplementation with 6 mg of melatonin administered 30 min prior to exercise) and a control group (placebo
without melatonin). They performed a continuous exercise of high intensity (135 beats/min). Samples
were collected 30 min before the exercise and 3, 15 and 60 min during the exercise. The results indicated
that the acute sport training presented: a) increased lipid peroxidation products (MDA) in both groups, control and experimental, with levels significantly decreased in the group treated with melatonin after 15 and
60 min of high-intensity exercise, b) the total antioxidant activity (TAS) was lower in the control group
than in the experimental, the latter showing significant differences at 60 min of high-intensity exercise c)
the lipid profile of subjects in the experimental group showed lower triglyceride levels than the control
group after 15 and 60 min of high-intensity exercise, d) immunological studies only showed, in the experimental group, an increase in IgA levels at 60 min after the exercise, and finally there were no significant differences between the groups for any of the other variables. In conclusion these results indicated that
treatment with melatonin in acute sports exercise reversed oxidative stress, improved defenses and lipid metabolism, which would result in an improvement in fitness
Maldonado y Aibar, M.D., Manfredi, M., Ribas Serna, J., Garcia-Moreno, H. y Calvo Gutiérrez, J.R. (2012). Melatonin administrated immediately before an intense exercise reverses oxidative stress, improves immunological defenses and lipid metabolism in football players. Physiology & Behavior, 105 (5), 1099-1103. https://doi.org/10.1016/j.physbeh.2011.12.015.
0031-9384
https://hdl.handle.net/11441/155350
10.1016/j.physbeh.2011.12.015
Acute sport training
Melatonin
Exercise
Immunity
Lipids
Oxidative stress
Human serum
Melatonin administrated immediately before an intense exercise reverses oxidative stress, improves immunological defenses and lipid metabolism in football players
oai:idus.us.es:11441/1559092024-03-06T19:02:54Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11029com_11441_11044col_11441_10990col_11441_11030col_11441_11045
00925njm 22002777a 4500
dc
2019
Background: Immune escape of tumor cells is a new hallmark of cancer in general, and breast cancer, in
particular. Previous studies have demonstrated that the immunological profile in peripheral blood may be a
prognostic and/or predictive biomarker in breast cancer. Thus, higher number of regulatory T cells (Tregs)
in blood from patients with breast cancer has been reported in relation to normal donors. In the present
study, we planned to evaluate the changes in different cell populations in peripheral blood: neutrophils,
monocytes and lymphocytes, as well as lymphocyte subpopulations [natural killer (NK), B lymphocytes,
T lymphocytes, both CD4+
and CD8+
, and Tregs] from patients with local breast cancer (both Her2+
and
Her2−
), before, during and after neoadjuvant chemotherapy.
Methods: We have employed flow cytometry for the cell analysis of fresh samples obtained before and
whilst the neoadjuvant treatment was accomplished. We have studied 50 successive patients from the Breast
Cancer Unit of the Virgen Macarena University Hospital during 2 years.
Results: Neoadjuvant chemotherapy induced a significant reduction in B cells, especially in Her2−
patients,
and a reduction in NK cells. CD4+
T cells decreased, whereas CD8+
cells only decreased in Her2−
patients.
Tregs were also diminished, especially in Her2+
patients, in response to treatment. Thus, higher CD8/Treg
ratio was observed in Her2+
patients. A higher percentage of Her2+
patients (66.6%) achieved complete
response than Her2−
patients (27.5%). Monocytes and neutrophils were not changed in peripheral blood.
Conclusions: Even though the decrease in B cells and NK cells in response to chemotherapy may be
deleterious in the neoadjuvant treatment of breast cancer, the decrease in Tregs and CD4 T cells, but not
CD8 T cells, increasing the CD8/Treg ratio, especially in Her2+
patients, may reveal a new tool to monitor
the immune response in breast cancer treated with chemotherapy in the neoadjuvant setting.
Sánchez Margalet, V., Barco Sánchez, A., Vilariño García, T., Jiménez Cortegana, C., Pérez Pérez, A., Henao Carrasco, F.M. y Lobo Acosta, M.Á. (2019). Circulating regulatory T cells from breast cancer patients in response to neoadjuvant chemotherapy. Translational Cancer Research, 8 (1), 59-65. https://doi.org/10.21037/tcr.2018.12.30.
2218-676X
2219-6803
https://hdl.handle.net/11441/155909
10.21037/tcr.2018.12.30
Breast cancer
Neoadjuvant therapy
Lymphocyte subpopulations
Regulatory T cells (Tregs)
Effector T lymphocytes
Circulating regulatory T cells from breast cancer patients in response to neoadjuvant chemotherapy
oai:idus.us.es:11441/1557572024-03-01T14:34:20Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11044col_11441_10990col_11441_11045
00925njm 22002777a 4500
dc
2023
SARS-CoV-2 infection is the cause of the disease named COVID-19, a major
public health challenge worldwide. Differences in the severity, complications and
outcomes of the COVID-19 are intriguing and, patients with similar baseline
clinical conditions may have very different evolution. Myeloid-derived suppressor
cells (MDSCs) have been previously found to be recruited by the SARS-CoV-2
infection and may be a marker of clinical evolution in these patients. We have
studied 90 consecutive patients admitted in the hospital before the vaccination
program started in the general population, to measure MDSCs and lymphocyte
subpopulations at admission and one week after to assess the possible
association with unfavorable outcomes (dead or Intensive Care Unit
admission). We analyzed MDSCs and lymphocyte subpopulations by flow
cytometry. In the 72 patients discharged from the hospital, there were
significant decreases in the monocytic and total MDSC populations measured
in peripheral blood after one week but, most importantly, the number of MDSCs (total and both monocytic and granulocytic subsets) were much higher in the 18
patients with unfavorable outcome. In conclusion, the number of circulating
MDSCs may be a good marker of evolution in the follow-up of unvaccinated
patients admitted in the hospital with the diagnosis of COVID-19.
Jiménez Cortegana, C., Salamanca, E., Palazón Carrión, N., Sánchez Jiménez, F., Pérez Pérez, A., Vilariño García, T.,...,Sánchez Margalet, V. (2023). Circulating myeloid-derived suppressor cells may be a useful biomarker in the follow-up of unvaccinated COVID-19 patients after hospitalization. Frontiers in Immunology, 14, 1266659. https://doi.org/10.3389/fimmu.2023.1266659.
1664-3224
https://hdl.handle.net/11441/155757
10.3389/fimmu.2023.1266659
SARS-CoV-2
COVID-19
MDSCs
T lymphocytes
Biomarker
Circulating myeloid-derived suppressor cells may be a useful biomarker in the follow-up of unvaccinated COVID-19 patients after hospitalization
oai:idus.us.es:11441/1563762024-03-18T13:47:04Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2012
Background: The management of surgical bleeding during
a face transplant in a patient diagnosed with bilateral neurofi bromatosis is quite complex. With the actual methods and
technology for hemostasis management, it may not always be
possible to give the clinician the support needed to manage
operative associated bleeding. Bedside hemostasis monitors
are needed urgently to assist clinicians in making the correct
diagnosis in a timely manner.
Methods: Our Mobile Laboratory Unit is a disruptive
solution for hemostasis management during major surgery
as it allows real-time monitoring, the predominant mechanism of bleeding and goal-direct coagulation therapy. The
unit is an autonomous mobile platform that can be moved
immediately to anywhere its service is needed and offers
a complete fl exible laboratory test which includes biochemistry, hematology and coagulation studies as standard
equipment. Results: In our case the test performed by the unit allowed
us to identify the reason for our patient ’ s bleeding at the
bedside. Severely decreased clot fi rmness of the fi brin-based
clot and a less impaired fi rmness of the whole blood clot,
suggested an acceptable contribution of platelets to the clot
quality, but decreased polymerization of fi brinogen into
fi brin.
Conclusions: In our opinion new insights into the pathophysiology of coagulopathy, the availability of technology such
as our Mobile Laboratory Unit, and awareness of side effects
of intravenous fl uids should encourage the idea that perhaps
it is time to change hemostasis management in operationrelated bleeding.
León Justel, A., Noval-Padillo, J.A., Polonio, F., Gomez-Cia, T., Hinojosa, R., Porras, M. y Guerrero Montávez, J.M. (2012). Mobile Laboratory Unit: A disruptor solution for hemostasis management during major surgery. Usage in the context of face transplantation. Clinical Chemistry and Laboratory Medicine, 50 (9), 1621-1624. https://doi.org/10.1515/cclm-2011-0764.
1434-6621
https://hdl.handle.net/11441/156376
10.1515/cclm-2011-0764
Bleeding surgery
Face transplant
Hemostasis management
Mobile Laboratory Unit
Point-of-care testing
Mobile Laboratory Unit: A disruptor solution for hemostasis management during major surgery. Usage in the context of face transplantation
oai:idus.us.es:11441/1305012024-02-14T20:28:21Zcom_11441_10813com_11441_10802com_11441_10690com_11441_10989com_11441_10983com_11441_11034col_11441_10814col_11441_10990col_11441_11035
00925njm 22002777a 4500
dc
2017
Objective-Extracellular nicotinamide phosphoribosyltransferase (eNAMPT) mediates inflammatory and potentially proatherogenic effects, whereas the role of intracellular NAMPT (iNAMPT), the rate limiting enzyme in the salvage pathway of nicotinamide adenine dinucleotide (NAD)+ generation, in atherogenesis is largely unknown. Here we investigated the effects of iNAMPT overexpression in leukocytes on inflammation and atherosclerosis. Approach and Results-Low-density lipoprotein receptor-deficient mice with hematopoietic overexpression of human iNAMPT (iNAMPThi), on a western type diet, showed attenuated plaque burden with features of lesion stabilization. This anti-atherogenic effect was caused by improved resistance of macrophages to apoptosis by attenuated chemokine (C-C motif) receptor 2-dependent monocyte chemotaxis and by skewing macrophage polarization toward an anti-inflammatory M2 phenotype. The iNAMPThi phenotype was almost fully reversed by treatment with the NAMPT inhibitor FK866, indicating that iNAMPT catalytic activity is instrumental in the atheroprotection. Importantly, iNAMPT overexpression did not induce any increase in eNAMPT, and eNAMPT had no effect on chemokine (C-C motif) receptor 2 expression and promoted an inflammatory M1 phenotype in macrophages. The iNAMPT-mediated effects at least partly involved sirtuin 1-dependent molecular crosstalk of NAMPT and peroxisome proliferator-activated receptor γ. Finally, iNAMPT and peroxisome proliferator-activated receptor γ showed a strong correlation in human atherosclerotic, but not healthy arteries, hinting to a relevance of iNAMPT/peroxisome proliferator-activated receptor γ pathway also in human carotid atherosclerosis. Conclusions-This study highlights the functional dichotomy of intracellular versus extracellular NAMPT, and unveils a critical role for the iNAMPT-peroxisome proliferator-activated receptor γ axis in atherosclerosis.
Bermúdez Pulgarín, B., Dahl, T.B., Medina, I., Groeneweg, M., Holm, S., Montserrat de la Paz, S.,...,Biessen, E.A.L. (2017). Leukocyte Overexpression of Intracellular NAMPT Attenuates Atherosclerosis by Regulating PPARγ-Dependent Monocyte Differentiation and Function. Arteriosclerosis, Thrombosis, and Vascular Biology, 37 (6), 1157-1167.
1079-5642
1524-4636
https://hdl.handle.net/11441/130501
10.1161/ATVBAHA.116.308187
Apoptosis
Atherosclerosis
Diet
Inflammation
Phenotype
Leukocyte Overexpression of Intracellular NAMPT Attenuates Atherosclerosis by Regulating PPARγ-Dependent Monocyte Differentiation and Function
oai:idus.us.es:11441/1553142024-02-16T17:27:54Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2022
Background: Recently, the study of hydrolysates from food proteins has been increasingly due to their wide range
of biological activities. Hydrolysates contain peptides of 2–20 amino acids that are inactive within the sequence
of the parent protein, but, once released after proteolytic processes, they exert numerous beneficial health effects.
Hemp, the non-drug variety of Cannabis sativa, is known as an important source of bioactive peptides due to the
high quality of hempseeds protein (20–25%) and well-balanced amino acid profile. For this reason, during the
last decade, numerous investigations have searched to elucidate the beneficial effects on the health of these
hempseed protein hydrolysates.
Scope and approach: The aim of this review was to collect all the scientific evidence on the demonstrated
beneficial effects of hempseed protein hydrolysates (HHs).
Key findings and conclusions: HHs have showed to possess antioxidant, immunomodulatory, hypotensive, hypo-
glycemic, and lipid-lowering capacities in vitro systems. All these effects have pointed out HHs as future
ingredient for the development of functional foods or dietary supplements useful for the prevention of chronic
diseases such as metabolic syndrome, diabetes or hypertension. However, few studies have evaluated the in vivo
effects of HHs. For this reason, further studies carried out in animal models or human are necessary to better
exploit the use of HHs for the development of new dietary supplements.
Santos-Sánchez, G., Álvarez-López, A.I., Ponce España, E., Carrillo Vico, A., Bollati, C. y Cruz-Chamorro, I. (2022). Hempseed (Cannabis sativa) protein hydrolysates: A valuable source of bioactive peptides with pleiotropic health-promoting effects. friends in food science and tecnology, 127, 303-318. https://doi.org/10.1016/j.tifs.2022.06.005.
0924-2244
https://hdl.handle.net/11441/155314
10.1016/j.tifs.2022.06.005
Bioactive peptide
Antioxidant
Inflammation
Hypertension
Hyperglycemia
Hypercholesterolemia
Hempseed (Cannabis sativa) protein hydrolysates: A valuable source of bioactive peptides with pleiotropic health-promoting effects
oai:idus.us.es:11441/1518842024-02-13T09:36:04Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2021
The study investigated the phenols, sugar and the antioxidant capacities of date fruit
extracts obtained by organic solvents and by hydrothermal treatment from six different Algerian
cultivars at two ripening stages for the first time. The analyzed cultivars exhibited potent antioxidant
properties (ferric reducing antioxidant power (FRAP), 1,1-Diphenyl-2-picrylhydrazyl (DPPH) and
2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) scavenging capacities) and different
phenols regardless of the solvents and the maturity stages. About 18 phenols were identified and
quantified, mainly in the hydrothermal extracts. The earlier stages were characterized by high
amounts of o-coumaric acid, cinnamic acid and luteolin, with a noticeable absence of quercetin. The
tamr stage presented the highest sugar content (78.15–86.85 mg/100 mg dry weight (DW)) with an
abundance of glucose. Galactose was present only in some cultivars from the kimri stage (tamjouhert).
Uronic acids were mostly detected at the tamr stage (4.02–8.82 mg gallic acid equivalent/100 mg
dried weight). The obtained results highlight the potential of using date fruit extracts as natural
antioxidants, especially at industrial scales that tend use hydrothermal extraction.
Tassoult, M., Kati, D. ., Fernández Prior, Á., Bermúdez-Oria, A., Fernández-Bolanos, J. y Rodríguez-Gutiérrez, G. (2021). Antioxidant Capacity and Phenolic and Sugar Profiles of Date Fruits Extracts from Six Different Algerian Cultivars as Influenced by Ripening Stages and Extraction Systems. Foods, 10 (3), 503. https://doi.org/10.3390/foods10030503.
2304-8158
https://hdl.handle.net/11441/151884
10.3390/foods10030503
Phoenix dactylifera L.
Secondary dates
Phenolic profile
Antioxidant activities
Sugars
Antioxidant Capacity and Phenolic and Sugar Profiles of Date Fruits Extracts from Six Different Algerian Cultivars as Influenced by Ripening Stages and Extraction Systems
oai:idus.us.es:11441/1458012024-02-14T09:07:48Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2022-10-27
Cancer affects more than 19 million people and is the second leading cause of death in the world. One of the principal strategies used in cancer therapy is the inhibition of topoisomerase II, involved in the survival of cells. Side effects and adverse reactions limit the use of topoisomerase II inhibitors; hence, research is focused on discovering novel compounds that can inhibit topoisomerase II and have a safer toxicological profile. Marine organisms are a source of secondary metabolites with different pharmacological properties including anticancer activity. The objective of this review is to present and discuss the pharmacological potential of marine-derived compounds whose antitumor activity is mediated by topoisomerase II inhibition. Several compounds derived from sponges, fungi, bacteria, ascidians, and other marine sources have been demonstrated to inhibit topoisomerase II. However, some studies only report docking interactions, whereas others do not fully explain the mechanisms of topoisomerase II inhibition. Further in vitro and in vivo studies are needed, as well as a careful toxicological profile evaluation with a focus on cancer cell selectivity.
Greco, G., Pellicioni, V., Cruz Chamorro, I., Attisani, G., Stefanelli, C. y Fimognari, C. (2022). Marine-Derived Compounds Targeting Topoisomerase II in Cancer Cells A Review. Marine Drugs, 20 (11), 674. https://doi.org/10.3390/md20110674.
1660-3397
https://hdl.handle.net/11441/145801
10.3390/md20110674
topoisomerase II
cancer chemotherapy
marine compounds
sponges
marine fungi
marine bacteria
marine invertebrates
Marine-Derived Compounds Targeting Topoisomerase II in Cancer Cells A Review
oai:idus.us.es:11441/1016162024-02-13T20:16:01Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2020-08-16
Inflammation is an essential immune response for the maintenance of tissue homeostasis. In a general sense, acute and chronic inflammation are different types of adaptive response that are called into action when other homeostatic mechanisms are insufficient. Although considerable progress has been made in understanding the cellular and molecular events that are involved in the acute inflammatory response to infection and tissue injury, the causes and mechanisms of systemic chronic inflammation are much less known. The pathogenic capacity of this type of inflammation is puzzling and represents a common link of the multifactorial diseases, such as cardiovascular diseases and type 2 diabetes. In recent years, interest has been raised by the discovery of novel mediators of inflammation, such as microRNAs and adipokines, with different effects on target tissues. In the present review, we discuss the data emerged from research of leptin in obesity as an inflammatory mediator sustaining multifactorial diseases and how this knowledge could be instrumental in the design of leptin-based manipulation strategies to help restoration of abnormal immune responses. On the other direction, chronic inflammation, either from autoimmune or infectious diseases, or impaired microbiota (dysbiosis) may impair the leptin response inducing resistance to the weight control, and therefore it may be a cause of obesity. Thus, we are reviewing the published data regarding the role of leptin in inflammation, and the other way around, the role of inflammation on the development of leptin resistance and obesity.
Pérez Pérez, A., Sánchez Jiménez, F., Vilariño García, T. y Sánchez Margalet, V. (2020). Role of leptin in inflammation and vice versa. International Journal of Molecular Sciences, 21 (5887), 1-24.
1422-0067
https://hdl.handle.net/11441/101616
doi:10.3390/ijms21165887
Leptin
Inflammation
Obesity
Leptin resistance
Microbiota
Role of leptin in inflammation and vice versa
oai:idus.us.es:11441/1461532024-02-17T17:27:38Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2020
La prevalencia de la esclerosis múltiple (EM) en los países asiáticos se considera
que es más baja que en los países occidentales, las poblaciones asiáticas tienen un 80% menos
de riesgo de EM que las caucásicas. No se conocen bien las cifras de incidencia, prevalencia
y su relación con otros países de su entorno y su relación con factores étnicos, ambientales y
socioeconómicos.
Hemos realizado una revisión bibliográfica exhaustiva de los datos epidemiológicos existentes
en China y países limítrofes, para estudiar la frecuencia de la enfermedad, centrándonos en la
prevalencia, sus cambios evolutivos a lo largo del tiempo y su relación con factores de género,
ambientales, alimenticios y socioculturales.
La prevalencia en China oscila en cifras que van desde 0,88 en 1986 a 5,2 en 2013 por 100.000
habitantes con una tendencia a aumentar que no es estadísticamente significativa (p = 0,08),
mientras que en Japón este incremento es muy significativo, con cifras que oscilan entre 8,1
y 18,6 (p < 0,001). La prevalencia en los países donde predomina la raza caucásica son mucho
más elevadas y aumentan con el tiempo, llegando a 115 por 100.000 habitantes en 2015 (r2 =
0,79, p = 0,0001).
En conclusión, la prevalencia de la EM en China parece está aumentando en los últimos años,
aunque la raza amarilla (chinos y japoneses, entre otros) tienen menor riesgo de padecerla que
el resto de las poblaciones. La latitud no parece ser un factor muy determinante en Asia para
presentar un mayor riesgo de padecer EM.
The prevalence of multiple sclerosis (MS) in Asian countries is thought to be lower
than in Western countries, with Asian populations presenting 80% less risk of MS than white
populations. Incidence and prevalence rates in Asian countries are therefore not well defined
and their association with rates in neighboring countries, as well as with ethnic, environmental,
and socioeconomic factors, are not well understood.
We performed a comprehensive literature review of epidemiological data from China and
neighbouring countries to study the frequency of the disease, focusing on prevalence, and the
progression over time and the influence of sex-related, environmental, dietary, and sociocul-
tural factors.
Prevalence rates in China range between 0.88 cases/100,000 population in 1986 and 5.2
cases/100,000 population in 2013, with a non-significant upwards trend (p = .08). The increase
observed in Japan, where figures ranged between 8.1 and 18.6 cases/100,000 population was
highly significant (p < .001). Prevalence rates in countries with predominantly white populations
are considerably higher and have increased over time, reaching 115 cases/100,000 population
in 2015 (r2 = 0.79, p < .0001).
In conclusion, the prevalence of MS in China appears to have risen in recent years, although
Asian populations (including Chinese and Japanese populations, among others) appear to pre-
sent less risk than other populations. Within Asia, geographical latitude appears not to be a
determining factor for developing MS.
Zhang, G.X., Carrillo Vico, A., Zhang, W.T., Gao, S.S. y Izquierdo Ayuso, G. (2020). Incidencia y prevalencia de la esclerosis múltiple en China y países asiáticos. Neurología, 38 (3), 159-172. https://doi.org/10.1016/j.nrl.2020.07.022.
0213-4853
1578-1968
https://hdl.handle.net/11441/146153
10.1016/j.nrl.2020.07.022
Esclerosis múltiple
China
Prevalencia
Asia
Multiple sclerosis
Prevalence
Incidencia y prevalencia de la esclerosis múltiple en China y países asiáticos
oai:idus.us.es:11441/1557102024-02-29T16:38:53Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11009col_11441_10990col_11441_11010
00925njm 22002777a 4500
dc
1999
Maldonado y Aibar, M.D., De Llanos Peña, F., Otero Castello, M.D., Hidalgo Ardanaz, M.J. y Sobrino Toro, J.P. (1999). El hacer profesional en el aula. Revista de Enseñanza Universitaria, 1, 139-152.
1131-5245
https://hdl.handle.net/11441/155710
El hacer profesional en el aula
oai:idus.us.es:11441/233312024-02-15T07:27:30Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11094col_11441_10990col_11441_11095
00925njm 22002777a 4500
dc
2013
Álvarez Sánchez, N., Rodríguez Rodríguez, A., Lardone, P.J., Guerrero Montávez, J.M. y Carrillo Vico, A. (2013). Melatonin: buffering the immune system. International Journal of Molecular Sciences, 14, 8638-8683.
1422-0067
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3645767/pdf/ijms-14-08638.pdf
http://hdl.handle.net/11441/23331
https://idus.us.es/xmlui/handle/11441/23331
Melatonin: buffering the immune system
oai:idus.us.es:11441/1554962024-02-22T17:05:15Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2022
We have previously reported the in vitro hypocholesterolemic, anti-inflammatory, and antioxidant effects of Alcalase-generated lupin protein hydrolysate (LPH). Given that lipoprotein deposition, oxidative stress, and inflammation are the main components of atherogenesis, we characterized the LPH composition, in silico identified LPH–peptides with activities related to atherosclerosis, and evaluated the in vivo LPH effects on atherosclerosis risk factors in a mouse model of atherosclerosis. After 15 min of Alcalase hydrolysis, peptides smaller than 8 kDa were obtained, and 259 peptides out of 278 peptides found showed biological activities related to atherosclerosis risk factors. Furthermore, LPH administration for 12 weeks reduced the plasma lipids, as well as the cardiovascular and atherogenic risk indexes. LPH also increased the total antioxidant capacity, decreased endothelial permeability, inflammatory response, and atherogenic markers. Therefore, this study describes for the first time that LPH prevents the early stages of atherosclerosis.
Santos-Sánchez, G., Cruz-Chamorro, ., Álvarez-Ríos, A.I., Álvarez-Sánchez, N., Rodríguez-Ortiz, ., Lardone, P.J.,...,Carrillo Vico, A. (2022). Bioactive Peptides from Lupin (Lupinus angustifolius) Prevent the Early Stages of Atherosclerosis in Western Diet-Fed ApoE–/– Mice. journal of Agricultura and Food Chemistry, 70 (27), 8243-8253. https://doi.org/10.1021/acs.jafc.2c00809.
0021-8561
1520-5118
https://hdl.handle.net/11441/155496
10.1021/acs.jafc.2c00809
Alcalase
Bioactive peptides
Cholesterol
Inflammation
Lupin
Oxidative stress
Atherosclerosis
Bioactive Peptides from Lupin (Lupinus angustifolius) Prevent the Early Stages of Atherosclerosis in Western Diet-Fed ApoE–/– Mice
oai:idus.us.es:11441/1548172024-03-21T13:18:24Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11009com_11441_11094com_11441_11044col_11441_10990col_11441_11010col_11441_11095col_11441_11045
00925njm 22002777a 4500
dc
2014
Objective:
To prospectively evaluate the effect of weight loss after bariatric surgery on microvascular function in morbidly obese patients with and without metabolic syndrome (MetS).
Methods:
A cohort of morbidly obese patients with and without MetS was studied before surgery and after 12 months of surgery. Healthy lean controls were also examined. Microvascular function was assessed by postocclusive reactive hyperemia (PORH) at forearm skin evaluated by laser Doppler flowmetry (LDF). Cutaneous vascular conductance (CVC) was calculated from laser-Doppler skin blood flow and blood pressure. Regression analysis was performed to assess the contribution of different clinical, metabolic and biochemical parameters to microvascular function.
Results:
Before surgery, 62 obese patients, 39 with MetS and 23 without MetS, and 30 lean control subjects were analyzed. The absolute area under the hyperemic curve (AUCH) CVC of PORH was significantly decreased in obese patients compared with lean control subjects. One year after surgery, AUCH CVC significantly increased in patients free of MetS, including patients that had MetS before surgery. In contrast, AUCH CVC did not significantly change in patients in whom MetS persisted after surgery. Stepwise multivariate regression analysis showed that only changes in HDL cholesterol (HDL-C) and oxidized LDL (oxLDL) independently predicted improvement of AUCH after surgery. These two variables together accounted for 40.9% of the variability of change in AUCH CVC after surgery.
Conclusions:
Bariatric surgery could significantly improve microvascular dysfunction in obese patients, but only in patients free of MetS after surgery. Improvement of microvascular dysfunction is strictly associated to postoperative increase in HDL-C levels and decrease in oxLDL levels.
Martín Rodríguez, J.F., Cervera Barajas, A., Madrazo Atutxa, A., García Luna, P.P., Pereira Cunill, J.L., Castro Luque, J.,...,Cano, D.A. (2014). Effect of bariatric surgery on microvascular dysfunction associated to metabolic syndrome: a 12-month prospective study. International Journal of Obesity, 38 (11), 1410-1415. https://doi.org/10.1038/ijo.2014.15.
0307-0565 (impreso)
1476-5497 (electrónico)
https://hdl.handle.net/11441/154817
10.1038/ijo.2014.15
Effect of bariatric surgery on microvascular dysfunction associated to metabolic syndrome: a 12-month prospective study
oai:idus.us.es:11441/1396892022-11-22T15:48:43Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2021-11-19
Cardiovascular disease (CVD) is the most common cause of morbidity and mortality in developed countries. The prevalence of CVD is much higher in patients with type 2 diabetes mellitus (T2DM), who may benefit from lifestyle changes, which include adapted diets. In this review, we provide the role of different groups of nutrients in patients with T2DM and CVD, as well as dietary approaches that have been associated with better and worse outcomes in those patients. Many different diets and supplements have proved to be beneficial in T2DM and CVD, but further studies, guidelines, and dietary recommendations are particularly required for patients with both diseases.
Jiménez Cortegana, C., Iglesias, P., Ribalta, J., Vilariño García, T., Montañez, L., Arrieta, F.,...,Sánchez Margalet, V. (2021). Nutrients and Dietary Approaches in Patients with Type 2 Diabetes Mellitus and Cardiovascular Disease: A Narrative Review. Nutrients, 13 (11), 4150. https://doi.org/10.3390/nu13114150.
2072-6643
https://hdl.handle.net/11441/139689
10.3390/nu13114150
type 2 diabetes
caridovascular risk
nutrients
diets
Nutrients and Dietary Approaches in Patients with Type 2 Diabetes Mellitus and Cardiovascular Disease: A Narrative Review
oai:idus.us.es:11441/1518822024-02-17T16:39:45Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2021
Hydroxytyrosol (HT) is the component primarily responsible for the neuroprotective effect
of extra virgin olive oil (EVOO). However, it is less effective on its own than the demonstrated
neuroprotective effect of EVOO, and for this reason, it can be postulated that there is an interaction
between several of the polyphenols of EVOO. The objective of the study was to assess the possible
interaction of four EVOO polyphenols (HT, tyrosol, dihydroxyphenylglycol, and oleocanthal) in
an experimental model of hypoxia-reoxygenation in rat brain slices. The lactate dehydrogenase
(LDH) efflux, lipid peroxidation, and peroxynitrite production were determined as measures of cell
death, oxidative stress, and nitrosative stress, respectively. First, the polyphenols were incubated
with the brain slices in the same proportions that exist in EVOO, comparing their effects with those
of HT. In all cases, the cytoprotective and antioxidant effects of the combination were greater than
those of HT alone. Second, we calculated the concentration–effect curves for HT in the absence or
presence of each polyphenol. Tyrosol did not significantly modify any of the variables inhibited
by HT. Dihydroxyphenylglycol only increased the cytoprotective effect of HT at 10 μM, while it
increased its antioxidant effect at 50 and 100 μM and its inhibitory effect on peroxynitrite formation
at all the concentrations tested. Oleocanthal increased the cytoprotective and antioxidant effects of
HT but did not modify its inhibitory effect on nitrosative stress. The results of this study show that
the EVOO polyphenols DHPG and OLC increase the cytoprotective effect of HT in an experimental
model of hypoxia-reoxygenation in rat brain slices, mainly due to a possibly synergistic effect on
HT’s antioxidant action. These results could explain the greater neuroprotective effect of EVOO than
of the polyphenols alone.
Cruz Cortés, J.P.d.l., Pérez de Algaba, ., Martín-Aurioles, ., Monsalud Arrebola, M., Ortega-Hombrados, L., Fernández Prior, Á. y Verdugo, C. (2021). Extra Virgin Oil Polyphenols Improve the Protective Effects of Hydroxytyrosol in an In Vitro Model of Hypoxia-Reoxygenation of Rat Brain. Brain Sciences, 11 (9), 1133. https://doi.org/10.3390/brainsci11091133.
2076-3425
https://hdl.handle.net/11441/151882
10.3390/brainsci11091133
Hydroxytyrosol
Extra virgin olive oil
Polyphenols
Neuroprotection
Extra Virgin Oil Polyphenols Improve the Protective Effects of Hydroxytyrosol in an In Vitro Model of Hypoxia-Reoxygenation of Rat Brain
oai:idus.us.es:11441/1384852024-02-14T20:31:30Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2022
SARS-CoV-2 specific T-cell response has been associated with disease severity, immune memory and heterologous response to endemic coronaviruses. However, an integrative approach combining a comprehensive analysis of the quality of SARS-CoV-2 specific T-cell response with antibody levels in these three scenarios is needed. In the present study, we found that, in acute infection, while mild disease was associated with high T-cell polyfunctionality biased to IL-2 production and inversely correlated with anti-S IgG levels, combinations only including IFN-γ with the absence of perforin production predominated in severe disease. Seven months after infection, both non-hospitalised and previously hospitalised patients presented robust anti-S IgG levels and SARS-CoV-2 specific T-cell response. In addition, only previously hospitalised patients showed a T-cell exhaustion profile. Finally, combinations including IL-2 in response to S protein of endemic coronaviruses were the ones associated with SARS-CoV-2 S-specific T-cell response in pre-COVID-19 healthy donors’ samples. These results could have implications for protective immunity against SARS-CoV-2 and recurrent COVID-19 and may help for the design of new prototypes and boosting vaccine strategies.
Perez-Gomez, A., Gasca-Capote, C., Vitalle, J., Ostos Marcos, F.J., Serna Gallego, A., Trujillo-Rodriguez, M.,...,Ruiz Mateos, E. (2022). Deciphering the quality of SARS-CoV-2 specific T-cell response associated with disease severity, immune memory and heterologous response. Clinical and translational medicine, 12 (4), e802. https://doi.org/10.1002/ctm2.802.
2001-1326
https://hdl.handle.net/11441/138485
10.1002/ctm2.802
COVID-19
Endemic coronaviruses
IL-2
Nucleocapsid
Polyfunctionality
SARS-CoV-2
Spike
T-cell response
Deciphering the quality of SARS-CoV-2 specific T-cell response associated with disease severity, immune memory and heterologous response
oai:idus.us.es:11441/161982024-02-14T13:59:28Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2002
En los últimos años ha habido un incremento de los malos
tratos y agresiones físicas a las mujeres; ello hace necesario
una agilización en el tratamiento social, médico y judicial
de estas pacientes.
Nuestro estudio describe y analiza los datos estadísticos
de las mujeres maltratadas que fueron atendidas en el Centro
de Urgencias y Especialidades Médicas del Ayuntamiento
de Sevilla durante el año 2000.
Escalera Rapela, M.M. y Maldonado y Aibar, M.D. (2002). No se golpea, no se lesiona, no se asesina ¡jamás!. Cuidados de urgencias en el maltrato a mujeres. Revista MAPFRE Medicina, 13, 99-109.
1130-5665
http://sid.usal.es/idocs/F8/ART6790/no.pdf
http://hdl.handle.net/11441/16198
https://idus.us.es/xmlui/handle/11441/16198
Mujeres maltratadas
profesionales de la salud
y centro de urgencias
No se golpea, no se lesiona, no se asesina ¡jamás!. Cuidados de urgencias en el maltrato a mujeres
oai:idus.us.es:11441/1283952024-02-14T11:13:22Zcom_11441_10813com_11441_10802com_11441_10690com_11441_10989com_11441_10983com_11441_11009col_11441_10814col_11441_10990col_11441_11010
00925njm 22002777a 4500
dc
2021
High-density lipoproteins (HDLs) play an important role in reverse cholesterol transport and present antioxidant properties, among others. In the central nervous system (CNS), there are HDLs, where these lipoproteins could influence brain health. Owing to the new evidence of HDL functionality remodeling in obese patients, and the fact that obesity-associated metabolic disturbances is pro-inflammatory and pro-oxidant, the aim of this study was to investigate if HDL functions are depleted in obese patients and obesity-associated microenvironment. HDLs were isolated from normal-weight healthy (nwHDL) and obese men (obHDL). The oxHDL level was measured by malondialdehyde and 4-hydroxynoneal peroxided products. BV2 microglial cells were exposed to different concentrations of nwHDL and obHDL in different obesity-associated pro-inflammatory microenvironments. Our results showed that hyperleptinemia increased oxHDL levels. In addition, nwHDLs reduced pro-inflammatory cytokines’ release and M1 marker gene expression in BV2 microglial cells. Nevertheless, both nwHDL co-administered with LPS+leptin and obHDL promoted BV2 microglial activation and a higher pro-inflammatory cytokine production, thus confirming that obesity-associated metabolic disturbances reverse the antioxidant and anti-inflammatory properties of HDLs in microglial cells.
Grao Cruces, E., Millán Linares, M.C., Martín Rubio, M.E., Toscano Sánchez, M.d.R., Barrientos Trigo, S., Bermúdez Pulgarín, B. y Montserrat de la Paz, S. (2021). Obesity-associated metabolic disturbances reverse the antioxidant and anti-inflammatory properties of high-density lipoproteins in microglial cells. Biomedicines, 9 (11), 1722.
2227-9059
https://hdl.handle.net/11441/128395
10.3390/biomedicines9111722
Lipoproteins
Microglia
Neuroinflammation
Obesity
Oxidation
Paraoxonase
Obesity-associated metabolic disturbances reverse the antioxidant and anti-inflammatory properties of high-density lipoproteins in microglial cells
oai:idus.us.es:11441/1554882024-02-22T15:40:31Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2023
Food allergy (FA) is an adverse immunological reaction to a specific food that can trigger a
wide range of symptoms from mild to life-threatening. This adverse reaction is caused by different
immunological mechanisms, such as IgE-mediated, non-IgE-mediated and mixed IgE-mediated
reactions. Its epidemiology has had a significant increase in the last decade, more so in developed
countries. It is estimated that approximately 2 to 10% of the world’s population has FA and this
number appears to be increasing and also affecting more children. The diagnosis can be complex
and requires the combination of different tests to establish an accurate diagnosis. However, the
treatment of FA is based on avoiding the intake of the specific allergenic food, thus being very
difficult at times and also controlling the symptoms in case of accidental exposure. Currently,
there are other immunomodulatory treatments such as specific allergen immunotherapy or more
innovative treatments that can induce a tolerance response. It is important to mention that research
in this field is ongoing and clinical trials are underway to assess the safety and efficacy of these
different immunotherapy approaches, new treatment pathways are being used to target and promote
the tolerance response. In this review, we describe the new in vitro diagnostic tools and therapeutic
treatments to show the latest advances in FA management. We conclude that although significant
advances have been made to improve therapies and diagnostic tools for FA, there is an urgent need
to standardize both so that, in their totality, they help to improve the management of FA.
Brasal-Prieto, M., Fernández-Prades, L., Dakhaoui, H., Sobrino, F., López Enriquez, S. y Palomares, F. (2023). Update on in vitro diagnostic tools and treatments for food allergies. Nutrients, 15 (17), 3744. https://doi.org/10.3390/nu15173744.
2072-6643
https://hdl.handle.net/11441/155488
10.3390/nu15173744
Food allergy
Immunology
Immunotherapy
Nanostructures
Probiotic
Herbal medicine
Update on in vitro diagnostic tools and treatments for food allergies
oai:idus.us.es:11441/1553442024-02-19T14:00:31Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2008
Melatonin is an indoleamine with a range of biological
and physiological properties, including those involved in
immune defense [1]. Melatonin acts on the immune system
by increasing natural killer cell activity [2–4] and Th2 cellmediated immune responses [5, 6]. Additionally, melatonin
regulates gene expression of several immunomodulatory
cytokines including interleukin (IL)-2 and interferon
(IFN)-c by human CD4-T cells [7]. The effect of melatonin
on the immune system is also supported by the existence of
specific binding sites for melatonin on lymphoid cells
Maldonado y Aibar, M.D. y Calvo Gutiérrez, J.R. (2008). Melatonin usage in ulcerative colitis: a case report. Journal Of Pineal Research, 45 (3), 339-340. https://doi.org/10.1111/j.1600-079X.2008.00584.x.
0742-3098
https://hdl.handle.net/11441/155344
10.1111/j.1600-079X.2008.00584.x
Melatonin usage in ulcerative colitis: a case report
oai:idus.us.es:11441/691892024-02-17T16:55:27Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2017
Background: Quantification of T-cell-receptor-excision circles (TRECs) and kappa-deleting-recombination-excision circles (KRECs) from dried blood spots (DBS) allows detection of neonates with severe T-cell and/or B-cell lymphopenia that are potentially affected by severe combined immunodeficiency (SCID), as well as X-linked agammaglobulinemia (XLA). Methods: Determination of TRECs and KRECs using a triplex RT-PCR (TRECS-KRECS-β-actin) assay from prospectively collected DBS between February 2014 and December 2016 in three hospitals in Seville, Spain. Cut-off levels were TRECs < 6/punch, KRECs < 4/punch and b-actin > 700/punch. Internal (SCID, XLA, ataxia telangiectasia) and external controls (CDC) were included. Results: A total of 8943 DBS samples obtained from 8814 neonates were analysed. Re-punching was necessary in 124 samples (1.4%) due to insufficient β-actin values (<700 copies/punch). Preterm neonates (GA < 37 weeks) and neonates with a BW < 2500 g showed significantly lower TRECs and KRECs levels (p < 0.001). Due to repeated pathological results, ten neonates were re-sampled (0.11%), of which five neonates (0.055%) confirmed the pathological results: one case was a fatal chromosomopathy (TRECs 1/KRECs 4); two were extreme premature newborns (TRECs 0/KRECs 0 and TRECs 1/KRECs 20 copies/punch); and 2 neonates were born to mothers receiving azathioprine during pregnancy (TRECs 92/KRECs 1 and TRECs 154/KRECs 3 copies/punch). All controls were correctly identified. Conclusions: Severe T- and B-cell lymphopenias were correctly identified by the TRECS-KRECS-β-actin assay. Prematurity and low BW are associated with lower TREC and KREC levels. Extreme prematurity and maternal immune suppressive therapy can cause false positive results of TRECs and KRECs values.
Felipe, B.d., Olbrich, P., Goycochea Valdivia, W., Delgado Pecellín, C., Sánchez Moreno, P., Sánchez Sánchez, B.,...,Salguero Martín de Soto, J. (2017). Newborn Screening for Primary T- and B-Cell Immune Deficiencies—A Prospective Study in Andalucía. International Journal of Neonatal Screening, 3 (4), 1-10.
2409-515X
https://hdl.handle.net/11441/69189
10.3390/ijns3040027
newborn screening
primary immunodeficiencies
TRECS
KRECS
Newborn Screening for Primary T- and B-Cell Immune Deficiencies—A Prospective Study in Andalucía
oai:idus.us.es:11441/1492122023-09-28T13:44:52Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2023-02-27
The immune-inflammatory, glucose homeostasis, and antioxidant response have a
crucial role in the prevention of non-communicable chronic diseases, according to the
World Health Organization (WHO). As people live longer in society, the lifestyle of human
changes and the prevalence of age-related degenerative diseases and obesity is likewise
rising quickly. One of the subfields of nutritional sciences, which covers the study of
nutrients and other food ingredients with an emphasis on how they affect mammalian
physiology, health, and behaviour, is nutritional biochemistry. Immunonutrition is a
new and interdisciplinary field within Nutritional Biochemistry since it encompasses
many elements of nutrition, immunity, inflammation, oxidation, and illnesses with an
immunometabolic basis. One of the promising ingredients that are currently being assessed
by scientists in terms of immunonutrition is food extracts.
Rivero-Pino, F., Millán-Linares, M.d.C. y Montserrat de la Paz, S. (2023). Current research on antioxidant, anti-inflammatory and anti-obesity potential of food extracts. FOODS, 12 (5). https://doi.org/10.3390/foods12051013.
2304-8158
https://hdl.handle.net/11441/149212
10.3390/foods12051013
Current research on antioxidant, anti-inflammatory and anti-obesity potential of food extracts
oai:idus.us.es:11441/1376922024-02-14T13:48:49Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11044col_11441_10990col_11441_11045
00925njm 22002777a 4500
dc
2022
Infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes a
disease (coronavirus disease 2019, COVID-19) that may develop into a systemic disease with
immunosuppression and death in its severe form. Myeloid-derived suppressive cells (MDSCs)
are inhibitory cells that contribute to immunosuppression in patients with cancer and infection.
Increased levels of MDSCs have been found in COVID-19 patients, although their role in the
pathogenesis of severe COVID-19 has not been clarified. For this reason, we raised the
question whether MDSCs could be useful in the follow-up of patients with severe COVID-19 in
the intensive care unit (ICU). Thus, we monitored the immunological cells, including MDSCs, in
80 patients admitted into the ICU. After 1, 2, and 3 weeks, we examined for a possible
association with mortality (40 patients). Although the basal levels of circulating MDSCs did not
discriminate between the two groups of patients, the last measurement before the endpoint
(death or ICU discharge) showed that patients discharged alive from the ICU had lower levels
of granulocytic MDSCs (G-MDSCs), higher levels of activated lymphocytes, and lower levels of
exhausted lymphocytes compared with patients who had a bad evolution (death). In
conclusion, a steady increase of G-MDSCs during the follow-up of patients with severe
COVID-19 was found in those who eventually died.
Jiménez Cortegana, C., Sánchez Jiménez, F., Pérez Pérez, A., Álvarez, N., Sousa, A., Cantón Bulnes, M.L.,...,Sánchez Margalet, V. (2022). Low levels of granulocytic myeloid-derived suppressor cells may be a good marker of survival in the follow-up of patients with severe COVID-19. Frontiers in Immunology, 12, 1-10. https://doi.org/10.3389/fimmu.2021.801410.
1664-3224
https://hdl.handle.net/11441/137692
10.3389/fimmu.2021.801410
SARS-CoV2
COVID-19
ICU
MDSCs
Tregs
PD-1
OX40
Low levels of granulocytic myeloid-derived suppressor cells may be a good marker of survival in the follow-up of patients with severe COVID-19
oai:idus.us.es:11441/1554692024-03-01T08:31:27Zcom_11441_10989com_11441_10983com_11441_10690com_11441_10994com_11441_11029col_11441_10990col_11441_10995col_11441_11030
00925njm 22002777a 4500
dc
2024-02
Dendritic cells (DCs) serve as professional antigen-presenting cells (APC) bridging innate and adaptive immunity, playing an essential role in triggering specific cellular and humoral responses against tumor and infectious antigens. Consequently, various DC-based antitumor therapeutic strategies have been developed, particularly vaccines, and have been intensively investigated specifically in the context of acute myeloid leukemia (AML). This hematological malignancy mainly affects the elderly population (those aged over 65), which usually presents a high rate of therapeutic failure and an unfavorable prognosis. In this review, we examine the current state of development and progress of vaccines in AML. The findings evidence the possible administration of DC-based vaccines as an adjuvant treatment in AML following initial therapy. Furthermore, the therapy demonstrates promising outcomes in preventing or delaying tumor relapse and exhibits synergistic effects when combined with other treatments during relapses or disease progression. On the other hand, the remarkable success observed with RNA vaccines for COVID-19, delivered in lipid nanoparticles, has revealed the efficacy and effectiveness of these types of vectors, prompting further exploration and their potential application in AML, as well as other neoplasms, loading them with tumor RNA.
Palomares, F., Pina, A., Dakhaoui, H., Leiva Castro, C., Munera Rodríguez, A.M., Cejudo Guillén, M.,...,López Enríquez, . (2024). Dendritic cells as a therapeutic strategy in acute myeloid leukemia: vaccines. Vaccines, 12 (2). https://doi.org/10.3390/vaccines12020165.
2076-393X
https://hdl.handle.net/11441/155469
10.3390/vaccines12020165
personalized medicine
immunotherapy
mRNA
COVID-19
dendritic cells
vaccines
Dendritic cells as a therapeutic strategy in acute myeloid leukemia: vaccines
oai:idus.us.es:11441/1253742024-02-13T20:09:48Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2021-04-22
: Kiwicha (Amaranthus caudatus) is considered one of the few multipurpose pseudocereals
for its potential use not only as a source of nutrients and fiber but also for its bioactive compounds.
In recent years, antioxidant peptides are commonly used as functional ingredient of food. Herein,
a kiwicha protein isolate (KPI), obtained from kiwicha defatted flour (KDF), was hydrolyzed by
Bioprotease LA 660, a food-grade endoprotease, under specific conditions. The resulting kiwicha
protein hydrolysates (KPHs) were chemically characterized and their digestibility and antioxidant
capacity were evaluated by in vitro cell-free experiments owing to their measure of capacity to
sequester DPPH free radical and reducing power. KPHs showed higher digestibility and antioxidant
capacity than intact proteins into KPI. Therefore, the results shown in this study indicate that KPHs
could serve as an adequate source of antioxidant peptides, representing an effective alternative to the
generation of functional food.
Montserrat de la Paz, S., Martínez‐López, A., Villanueva‐Lazo, Á., Pedroche, J., Millán, F. y Millán-Linares, M.d.C. (2021). Identification and characterization of novel antioxidant protein hydrolysates from kiwicha (amaranthus caudatus l.). Antioxidants, 10 (5)
2076-3921
https://hdl.handle.net/11441/125374
10.3390/antiox10050645
Kiwicha
Protein hydrolysate
Bioactive compound
Food ingredient
Antioxidant activity
Identification and characterization of novel antioxidant protein hydrolysates from kiwicha (amaranthus caudatus l.)
oai:idus.us.es:11441/1471702024-02-17T16:18:34Zcom_11441_10989com_11441_10983com_11441_10690com_11441_11029col_11441_10990col_11441_11030
00925njm 22002777a 4500
dc
2023-01-13
Nutraceuticals act as cellular and functional modulators, contributing to the homeostasis of
physiological processes. In an inflammatory microenvironment, these functional foods can interact
with the immune system by modulating or balancing the exacerbated proinflammatory response. In
this process, immune cells, such as antigen-presenting cells (APCs), identify danger signals and, after
interacting with T lymphocytes, induce a specific effector response. Moreover, this conditions their
change of state with phenotypical and functional modifications from the resting state to the activated
and effector state, supposing an increase in their energy requirements that affect their intracellular
metabolism, with each immune cell showing a unique metabolic signature. Thus, nutraceuticals,
such as polyphenols, vitamins, fatty acids, and sulforaphane, represent an active option to use
therapeutically for health or the prevention of different pathologies, including obesity, metabolic
syndrome, and diabetes. To regulate the inflammation associated with these pathologies, intervention
in metabolic pathways through the modulation of metabolic energy with nutraceuticals is an attractive
strategy that allows inducing important changes in cellular properties. Thus, we provide an overview
of the link between metabolism, immune function, and nutraceuticals in chronic inflammatory
processes associated with obesity and diabetes, paying particular attention to nutritional effects on
APC and T cell immunometabolism, as well as the mechanisms required in the change in energetic
pathways involved after their activation.
2072-6643
https://hdl.handle.net/11441/147170
10.3390/nu15020411
Tolerogenic dendritic cells
Macrophages
Regulatory T cells
Regulatory B cells
Proteosome
Autophagy
Succinate
Glycolysis
Oxidative phosphorylation
Tricarboxylic acid cycle
Nutraceuticals as potential therapeutic modulators in immunometabolism
oai:idus.us.es:11441/129612024-02-14T19:26:33Zcom_11441_10989com_11441_10983com_11441_10690com_11441_2781com_11441_2379com_11441_137148col_11441_10990col_11441_2813
00925njm 22002777a 4500
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2007
Este proyecto de innovación docente, pretendió adaptar la asignatura de Inmunología, de la Licenciatura de Bioquímica, al Espacio Europeo de Educación Superior. Para ello, fue necesario realizar una guía de programación docente que recogiese todas y cada unas de las actividades e innovaciones a realizar con los alumnos, sus objetivos, metodología y temporización para el desarrollo del aprendizaje y finalmente los resultados esperados.
This educational innovation project sought to adapt the subject of Immunology, from Biochemistry degree, to European Space of Higher Education. For it, was necessary to devise a guide of educational programming which to collect all the activities and innovations to carry out with the students, its objectives, methodology and timekeeping for the development of the learning and finally the results expected.
Maldonado y Aibar, M.D. (2007). El Espacio Europeo de Educación Superior (EEES) y su repercusión en los procesos de enseñanza aprendizaje de la inmunología. Revista de Enseñanza Universitaria, 29, 68-79.
1132-8983
http://institucional.us.es/revistas/universitaria/29/REU29(Maldonado68-79).pdf
http://hdl.handle.net/11441/12961
https://idus.us.es/xmlui/handle/11441/12961
El Espacio Europeo de Educación Superior (EEES) y su repercusión en los procesos de enseñanza aprendizaje de la inmunología
oai:idus.us.es:11441/1540362024-01-25T16:43:32Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
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2021
Food allergy is an increasing problem worldwide, with strict avoidance being classically the
only available reliable treatment. The main objective of this review is to cover the latest information
about the tools available for the diagnosis and treatment of food allergies. In recent years, many efforts
have been made to better understand the humoral and cellular mechanisms involved in food allergy
and to improve the strategies for diagnosis and treatment. This review illustrates IgE-mediated
food hypersensitivity and provides a current description of the diagnostic strategies and advances
in different treatments. Specific immunotherapy, including different routes of administration and
new therapeutic approaches, such as hypoallergens and nanoparticles, are discussed in detail. Other
treatments, such as biologics and microbiota, are also described. Therefore, we conclude that although
important efforts have been made in improving therapies for food allergies, including innovative
approaches mainly focusing on efficacy and safety, there is an urgent need to develop a set of basic
and clinical results to help in the diagnosis and treatment of food allergies.
2304-8158
https://hdl.handle.net/11441/154036
10.3390/foods10051037
food allergy
specific immunotherapy
non-specific therapy
hypoallergens
nanoparticles
microbiota
New Insights in Therapy for Food Allergy
oai:idus.us.es:11441/1517722024-02-13T09:38:53Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
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2021
The objective of this study was to assess a possible synergistic effect of two extra-virgin olive oil polyphenols, 3,4,-dyhydroxyphenylglycol (DHPG) and hydroxytyrosol (HT), in an experimental model of type 1 diabetes. Seven groups of animals were studied: (1) Nondiabetic rats (NDR), (2) 2-month-old diabetic rats (DR), (3) DR treated with 5 mg/kg/day p.o. HT, (4) DR treated with 0.5 mg/kg/day p.o. DHPG, (5) DR treated with 1 mg/kg/day p.o. DHPG, (6) DR treated with HT + DHPG (0.5), (7) DR treated with HT + DHPG (1). Oxidative stress variables (lipid peroxidation, glutathione, total antioxidant activity, 8-isoprostanes, 8-hydroxy-2-deoxyguanosine, and oxidized LDL), nitrosative stress (3-nitrotyrosine), and some cardiovascular biomarkers (platelet aggregation, thromboxane B2, prostacyclin, myeloperoxidase, and vascular cell adhesion protein 1 (VCAM-1)) were analyzed. The diabetic animals showed an imbalance in all of the analyzed variables. HT exerted an antioxidant and downregulatory effect on prothrombotic biomarkers while reducing the fall of prostacyclin. DHPG presented a similar, but quantitatively lower, profile. HT plus DHPG showed a synergistic effect in the reduction of oxidative and nitrosative stress, platelet aggregation, production of prostacyclin, myeloperoxidase, and VCAM-1. This synergism could be important for the development of functional oils enriched in these two polyphenols in the proportion used in this study.
Cortes, J., Vallejo-Carmona, L., Arrebola, M., Martin-Aurioles, E., Rodriguez-Perez, M., Ortega-Hombrados, L.,...,Gonzalez-Correa, J. (2021). Synergistic Effect of 3 ',4 '-Dihidroxifenilglicol and Hydroxytyrosol on Oxidative and Nitrosative Stress and Some Cardiovascular Biomarkers in an Experimental Model of Type 1 Diabetes Mellitus. Antioxidants, 10 (12), 1983. https://doi.org/10.3390/antiox10121983.
2076-3921
https://hdl.handle.net/11441/151772
10.3390/antiox10121983
Hydroxytyrosol
Extra-virgin olive oil polyphenols
3′,4′-dihidroxifenilglicol
Oxidative stress
Cardiovascular
Synergistic Effect of 3 ',4 '-Dihidroxifenilglicol and Hydroxytyrosol on Oxidative and Nitrosative Stress and Some Cardiovascular Biomarkers in an Experimental Model of Type 1 Diabetes Mellitus
oai:idus.us.es:11441/1045982024-02-14T11:10:13Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
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2021-01-15
In the framework of research aimed at promoting the nutraceutical properties of the phenolic
extract (BUO) obtained from an extra virgin olive oil of the Frantoio cultivar cultivated in Tuscany
(Italy), with a high total phenols content, this study provides a comprehensive characterization of
its antioxidant properties, both in vitro by Trolox equivalent antioxidant capacity, oxygen radical
absorbance capacity, ferric reducing antioxidant power, and 2,2-diphenyl-1-picrylhydrazyl assays,
and at the cellular level in human hepatic HepG2 and human intestinal Caco-2 cells. Notably, in
both cell systems, after H2O2 induced oxidative stress, the BUO extract reduced reactive oxygen
species, lipid peroxidation, and NO overproduction via modulation of inducible nitric oxide synthase
protein levels. In parallel, the intestinal transport of the different phenolic components of the
BUO phytocomplex was assayed on differentiated Caco-2 cells, a well-established model of mature
enterocytes. The novelty of our study lies in having investigated the antioxidant effects of a complex
pool of phenolic compounds in an extra virgin olive oil (EVOO) extract, using either in vitro assays
or liver and intestinal cell models, rather than the effects of single phenols, such as hydroxytyrosol
or oleuropein. Finally, the selective trans-epithelial transport of some oleuropein derivatives was
observed for the first time in differentiated Caco-2 cells.
Bartolomei, M., Bollati, C., Bellumori, M., Cecchi, L., Cruz-Chamorro, I., Santos-Sánchez, G. y Lammi, C. (2021). Extra virgin olive oil phenolic extract on human hepatic hepg2 and intestinal caco-2 cells: assessment of the antioxidant activity and intestinal trans-epithelial transport. Antioxidants, 10, 1-19.
2076-3921
https://hdl.handle.net/11441/104598
10.3390/antiox10010118
Phenolic Phytocomplex
ROS
Lipid peroxidation
Secoiridoids
Hydroxytyrosol
Oleuropein aglycone
Trans-epithelial transport
Extra virgin olive oil phenolic extract on human hepatic hepg2 and intestinal caco-2 cells: assessment of the antioxidant activity and intestinal trans-epithelial transport
oai:idus.us.es:11441/1391872024-02-13T20:20:26Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
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2021-12
Background
To prevent the invasion and transmission of SARS-CoV-2, mRNA-based vaccines, non-replicating viral vector vaccines, and inactivated vaccines have been developed. The European Medicines Agency (EMA) authorized the use of the anti-SARS-CoV-2 vaccine in January 2021, the date on which the vaccination program began in Spain and across Europe. The aim of this study is to monitor the safety of anti-SARS-CoV-2 vaccines and report any cases of undesirable effects that have occurred, that are not included in the health profile of mRNA-based vaccines for commercialisation in humans. Furthermore, a brief review is given of the mechanism of action of the anti-SARS-CoV-2 vaccine on the host's immune system in triggering the reactivation of the herpes varicella-zoster infection.
Methods
Follow-up of patients under the care of the southern health district of Seville of the SAS (Andalusian Health Service) during the Spanish state of alarm over the COVID-19 pandemic.
Results
Two patients, a 79-year-old man and a 56-year-old woman, are reported who, after 4 and 16 days respectively of receiving the Pfizer-BNT162b2 vaccine against SARS-CoV-2, presented a state of reactivation of herpes varicella-zoster virus (VZV).
Discussion
The immunosenescence of the reported patients, together with the immunomodulation generated by administering the anti-SARS-CoV-2 vaccines, that depress certain cell subpopulations, could explain the awakening of VZV latency.
Maldonado y Aibar, M.D. y Romero Aibar, J. (2021). The Pfizer-BNT162b2 mRNA-based vaccine against SARS-CoV-2 may be responsible for awakening the latency of herpes varicella-zoster virus. Brain, Behavior, & Immunity - Health, 18, 100381. https://doi.org/10.1016/j.bbih.2021.100381.
2666-3546
https://hdl.handle.net/11441/139187
10.1016/j.bbih.2021.100381
The anti-SARS-CoV-2 vaccines
mRNA-based vaccines
Herpes zoster infection
Immune system
The Pfizer-BNT162b2 mRNA-based vaccine against SARS-CoV-2 may be responsible for awakening the latency of herpes varicella-zoster virus
oai:idus.us.es:11441/1473622024-02-14T08:44:37Zcom_11441_10813com_11441_10802com_11441_10690com_11441_10989com_11441_10983com_11441_11029col_11441_10814col_11441_10990col_11441_11030
00925njm 22002777a 4500
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2022
Seeds from non-drug varieties of hemp (Cannabis sativa L.) have been used for traditional
medicine, food, and fiber production. Our study shows that phytol obtained from hemp seed oil
(HSO) exerts anti-inflammatory activity in human monocyte-macrophages. Fresh human monocytes
and human macrophages derived from circulating monocytes were used to evaluate both plasticity
and anti-inflammatory effects of phytol from HSO at 10–100 mM using FACS analysis, ELISA, and
RT-qPCR methods. The quantitative study of the acyclic alcohol fraction isolated from HSO shows
that phytol is the most abundant component (167.59 ± 1.81 mg/Kg of HSO). Phytol was able to
skew monocyte-macrophage plasticity toward the anti-inflammatory non-classical CD14+CD16++
monocyte phenotype and toward macrophage M2 (CD200Rhigh and MRC-1high), as well as to reduce
the production of IL-1β, IL-6, and TNF-α, diminishing the inflammatory competence of mature
human macrophages after lipopolysaccharide (LPS) treatment. These findings point out for the first
time the reprogramming and anti-inflammatory activity of phytol in human monocyte-macrophages.
In addition, our study may help to understand the mechanisms by which phytol from HSO contributes
to the constant and progressive plasticity of the human monocyte-macrophage linage.
Claro Cala, C.M., Grao Cruces, E., Toscano Sánchez, M.d.R., Millán-Linares, M.d.C., Montserrat de la Paz, S. y Martín Rubio, M.E. (2022). Acyclic Diterpene Phytol from Hemp Seed Oil (Cannabis sativa L.) Exerts Anti-Inflammatory Activity on Primary Human Monocytes-Macrophages. Foods, 11 (15), 2366. https://doi.org/10.3390/foods11152366.
2304-8158
https://hdl.handle.net/11441/147362
10.3390/foods11152366
Phytol
Nutraceuticals
Unsaponifiable
Hemp seed oil
Functional foods
Immunonutrition
Acyclic Diterpene Phytol from Hemp Seed Oil (Cannabis sativa L.) Exerts Anti-Inflammatory Activity on Primary Human Monocytes-Macrophages
oai:idus.us.es:11441/1022752024-02-14T11:34:02Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
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2019
Objectives: To obtain greater knowledge of the extra-pineal sources of melatonin during development, the amount of indolamine and the
expression levels of the last two enzymes involved in its biosynthesis, Arylalkylamine N-acetyltransferase (AANAT) and acetylserotonin Omethyltransferase (ASMT), were analyzed in the human thymus from children from three different age groups (from days to years). The melatonin
membrane and nuclear receptor expression levels also were studied.
Methods: Quantitative reverse transcriptase PCR and western blot were performed to investigate the receptor and enzyme expression levels.
The results were examined and correlated with the ages of the thymuses.
Results: We found high levels of indolamine in the thymuses of newborns (younger than 1 month), which decreased during development;
thymuses from the months (from 2 to 11 months) and years (from 1 to 12 years) groups showed lower levels. A similar decline was also observed
in the mRNA of the AANAT enzyme and the expression levels of melatonin receptors. However, ASMT expression was exactly the opposite, with
low levels in the newborn group and higher levels in the years group. Our results show that the thymic synthesis of melatonin occurs very early in
childhood. Additionally, this is the first report that is focused on melatonin receptors expression in the human thymus.
Conclusion: Considering the limited melatonin synthesis performed by the newborn pineal gland, we suggest that the high levels of melatonin
found in human thymus in this experimental group arise from synthesis in the tissue itself, which could be contributing to the immune efficiency at
the thymic level.
Cruz Chamorro, I., Álvarez-Sánchez, N., Escalante-Andicoechea, C., Carrillo Vico, A., Rubio, A., Guerrero, J.M.,...,Lardone, P.J. (2019). Temporal expression patterns of the melatoninergic system in the human thymus of children. Molecular Metabolism, 28
2212-8778
https://hdl.handle.net/11441/102275
10.1016/j.molmet.2019.07.007
Melatonin
Thymus
AANAT
ASMT
Melatonin receptor
Nuclear receptor ROR-alpha
Temporal expression patterns of the melatoninergic system in the human thymus of children
oai:idus.us.es:11441/1394292024-02-14T11:15:41Zcom_11441_10989com_11441_10983com_11441_10690col_11441_10990
00925njm 22002777a 4500
dc
2021
This study investigated the immune mechanisms whereby administration of Bacteroides uniformis CECT 7771 reduces metabolic dysfunction in obesity. C57BL/6 adult male mice were fed a standard diet or a Western diet high in fat and fructose, supplemented or not with B. uniformis CECT 7771 for 14 weeks. B. uniformis CECT 7771 reduced body weight gain, plasma cholesterol, triglyceride, glucose, and leptin levels; and improved oral glucose tolerance in obese mice. Moreover, B. uniformis CECT 7771 modulated the gut microbiota and immune alterations associated with obesity, increasing Tregs and reducing B cells, total macrophages and the M1/M2 ratio in both the gut and epididymal adipose tissue (EAT) of obese mice. B. uniformis CECT 7771 also increased the concentration of the anti-inflammatory cytokine IL-10 in the gut, EAT and peripheral blood, and protective cytokines TSLP and IL-33, involved in Treg induction and type 2 innate lymphoid cells activation, in the EAT. It also restored the obesity-reduced TLR5 expression in the ileum and EAT. The findings indicate that the administration of a human intestinal bacterium with immunoregulatory properties on the intestinal mucosa helps reverse the immuno-metabolic dysfunction caused by a Western diet acting over the gut-adipose tissue axis.
Fabersani, E., Portune, K., Campillo, I., López-Almela, I., Montserrat de la Paz, S., Romaní-Pérez, M.,...,Sanz, Y. (2021). Bacteroides uniformis CECT 7771 alleviates inflammation within the gut-adipose tissue axis involving TLR5 signaling in obese mice. Scientific Reports, 11 (1). https://doi.org/10.1038/s41598-021-90888-y.
2045-2322
https://hdl.handle.net/11441/139429
10.1038/s41598-021-90888-y
Immunology
Microbiology
Physiology
Bacteroides uniformis CECT 7771 alleviates inflammation within the gut-adipose tissue axis involving TLR5 signaling in obese mice
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