2024-03-28T12:02:22Zhttps://idus.us.es/oai/requestoai:idus.us.es:11441/814612024-02-15T07:33:48Zcom_11441_11094com_11441_10983com_11441_10690com_11441_11044com_11441_10903com_11441_10802col_11441_11095col_11441_11045col_11441_10904
Morales Barroso, Isabel
López Cerero, Lorena
Navarro, María Dolores
Gutiérrez Gutiérrez, Belén
Pascual Hernández, Álvaro
Rodríguez-Baño, Jesús
2019-01-11T08:41:46Z
2019-01-11T08:41:46Z
2018-11-15
Morales Barroso, I., López Cerero, L., Navarro, M.D., Gutiérrez Gutiérrez, B., Pascual Hernández, Á. y Rodríguez Baño, J. (2018). Intestinal colonization due to Escherichia coli ST131: Risk factors and prevalence. Antimicrobial Resistance and Infection Control, 7, 135-1-135-6.
2047-2994
https://hdl.handle.net/11441/81461
10.1186/s13756-018-0427-9
Background Escherichia coli sequence type 131 (ST131) is a successful clonal group that has dramatically spread during the last decades and is considered an important driver for the rapid increase of quinolone resistance in E. coli. Methods Risk factors for rectal colonization by ST131 Escherichia coli (irrespective of ESBL production) were investigated in 64 household members (18 were colonized) and 54 hospital contacts (HC; 10 colonized) of 34 and 30 index patients with community and nosocomial infection due to these organisms, respectively, using multilevel analysis with a p limit of < 0.1. Result Colonization among household members was associated with the use of proton-pump inhibitors (PPI) by the household member (OR = 3.08; 95% CI: 0.88–10.8) and higher age of index patients (OR = 1.05; 95% CI; 1.01–1.10), and among HC, with being bed-ridden (OR = 21.1; 95% CI: 3.61–160.0) and having a urinary catheter (OR = 8.4; 95% CI: 0.87–76.9). Conclusion Use of PPI and variables associated with higher need of person-to-person contact are associated with increased risk of rectal colonization by ST131. These results should be considered for infection control purposes.
Plan Nacional de I + D + i 2013-2016
Ministerio de Economía y Competitividad (España)
European Development Regional Fund REIPI RD12/0015/0010 REIPI RD16/0016/0001
Instituto de Salud Carlos III 070190 AC16/000076-MODERN AC16/AC16/00072-ST131TS
Junta de Andalucía CTS5259 CTS210
application/pdf
eng
Biomed Central
Antimicrobial Resistance and Infection Control, 7, 135-1-135-6.
REIPI RD12/0015/0010
REIPI RD16/0016/0001
070190
AC16/000076-MODERN
AC16/AC16/00072-ST131TS
CTS5259
CTS210
http://dx.doi.org/10.1186/s13756-018-0427-9
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Escherichia coli
ST131
Intestinal colonisation
Risk factors
Carriage
Prevalence colonization
Outcome
Intestinal colonization due to Escherichia coli ST131: Risk factors and prevalence
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Instituto de Biomedicina de Sevilla (IBIS)
Universidad de Sevilla. Departamento de Medicina
Universidad de Sevilla. Departamento de Microbiología
Antimicrobial Resistance and Infection Control
7
135-1
135-6
https://ror.org/03yxnpp24
6 p.
ORIGINAL
Morales Barroso, I.a,b,c_Intestinal-colonization-due-to-Escherichia-coli-ST131-Risk-factors-and-prevalenceArticleOpen-Access_2018.pdf
Morales Barroso, I.a,b,c_Intestinal-colonization-due-to-Escherichia-coli-ST131-Risk-factors-and-prevalenceArticleOpen-Access_2018.pdf
application/pdf
594538
https://idus.us.es/bitstream/11441/81461/1/Morales%20Barroso%2c%20I.a%2cb%2cc_Intestinal-colonization-due-to-Escherichia-coli-ST131-Risk-factors-and-prevalenceArticleOpen-Access_2018.pdf
67303d74357dab0839f3ee796ddaeb95
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/81461/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/81461
oai:idus.us.es:11441/81461
2024-02-15 08:33:48.623
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1339302024-02-17T17:36:42Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Rosenberg, Tally
Jiménez Guerrero, Irene
Tamir Ariel, Dafna
Yarnitzky, Tali
Burdman, Saul
2022-06-01T10:56:06Z
2022-06-01T10:56:06Z
2022-05
Rosenberg, T., Jiménez Guerrero, I., Tamir Ariel, D., Yarnitzky, T. y Burdman, S. (2022). The GDSL-Lipolytic Enzyme Lip1 Is Required for Full Virulence of the Cucurbit Pathogenic Bacterium Acidovorax citrulli. Microorganisms, 10 (5), 1016.
2076-2607
https://hdl.handle.net/11441/133930
10.3390/microorganisms10051016
Bacterial fruit blotch caused by Acidovorax citrulli is a serious disease of cucurbit crops. Here we report characterization of a mutant strain of A. citrulli M6 defective in lip1, a gene encoding a lipolytic enzyme. The M6-lip1- mutant was detected in a mutant library screen aimed at identifying M6 mutants with altered levels of twitching motility. In this screen M6-lip1- was the only mutant that showed significantly larger twitching motility haloes around colonies than wild-type M6. Sequence analyses indicated that lip1 encodes a member of the GDSL family of secreted lipolytic enzymes. In line with this finding, lipolytic assays showed that the supernatants of M6-lip1- had lower lipolytic activity as compared with those of wild-type M6 and a lip1-complemented strain. The mutant was also affected in swimming motility and had compromised virulence on melon seedlings and on Nicotiana benthamiana leaves relative to wild-type and complemented strains. Lip1 contains a predicted N-terminal signal sequence for type II secretion. Evidence from our study confirms Lip1 is indeed secreted in a type II secretion-dependent manner, and this is required for full virulence of A. citrulli. To the best of our knowledge this is the first study reporting contribution of lipolytic activity to virulence of a plant-pathogenic Acidovorax species.
application/pdf
18 p.
eng
MDPI
Microorganisms, 10 (5), 1016.
https://dx.doi.org/10.3390/microorganisms10051016
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Acidovorax citrulli
bacterial fruit blotch
lipase
esterase
virulence
type IV pili
twitching motility
type II secretion
The GDSL-Lipolytic Enzyme Lip1 Is Required for Full Virulence of the Cucurbit Pathogenic Bacterium Acidovorax citrulli
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Microorganisms
10
5
1016
https://ror.org/03yxnpp24
ORIGINAL
pubmicroorganisms-10-01016.pdf
pubmicroorganisms-10-01016.pdf
application/pdf
3462590
https://idus.us.es/bitstream/11441/133930/1/pubmicroorganisms-10-01016.pdf
ccfb8e52a571368b7a54cf8be1e463af
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/133930/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/133930
oai:idus.us.es:11441/133930
2024-02-17 18:36:42.839
open access
idUS - Universidad de Sevilla
idus@us.es
RGVjbGFyYSBxdWU6CgphKSBFcyBhdXRvciB5IHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIHByb3BpZWRhZCBpbnRlbGVjdHVhbC4KCgpiKSBTaSBleGlzdGUgcHJldmlhIGNlc2nDs24gYSB0ZXJjZXJvcyBkZSBsb3MgZGVyZWNob3MgZGUgZXhwbG90YWNpw7NuIGRlIGxhIG9icmEsIGN1ZW50YSBjb24gbGEgYXV0b3JpemFjacOzbiBkZSBkaWNob3MgdGl0dWxhcmVzLiAKCgpjKSBTaSBlbCBkb2N1bWVudG8gY29udGllbmUgbWF0ZXJpYWxlcyBkZSBsb3MgcXVlIG5vIGVzIHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIGV4cGxvdGFjacOzbiwgIHRpZW5lIGVsIHBlcm1pc28gcGFyYSBkZXBvc2l0YXJsb3MgeSBxdWUgZXNlIG1hdGVyaWFsIGVzdMOhIGlkZW50aWZpY2FkbyBjbGFyYW1lbnRlLgoKCkVsIGF1dG9yIHJlYWxpemEgbGEgY2VzacOzbiBncmF0dWl0YSB5IG5vIGV4Y2x1c2l2YSBhIGxhIFVuaXZlcnNpZGFkIGRlIFNldmlsbGEgZGUgbG9zIGRlcmVjaG9zIGRlIHJlcHJvZHVjY2nDs24sIGRpc3RyaWJ1Y2nDs24sIGNvbXVuaWNhY2nDs24gcMO6YmxpY2EgeSB0cmFuc2Zvcm1hY2nDs247IHBlcm1pdGllbmRvIGFsIHJlcG9zaXRvcmlvOgoKCglUcmFuc2Zvcm1hY2nDs24gZGVsIGZvcm1hdG8gcGFyYSBzdSBpbmNvcnBvcmFjacOzbiB5IHByZXNlcnZhY2nDs24uCgoKCVJlcHJvZHVjY2nDs24geSBhcmNoaXZvICBlbiBsb3Mgc2Vydmlkb3JlcyBhc29jaWFkb3MgYWwgcmVwb3NpdG9yaW8uCgoKCVN1IGNvbXVuaWNhY2nDs24gcMO6YmxpY2EgeSBzdSBwdWVzdGEgYSBkaXNwb3NpY2nDs24gZGUgbW9kbyBsaWJyZSB5IGdyYXR1aXRvIGEgdHJhdsOpcyBkZWwgYXJjaGl2byBhYmllcnRvIGluc3RpdHVjaW9uYWwKCgpFbCBhdXRvciBhdXRvcml6YSBxdWUgbGEgb2JyYSBzZSBwb25nYSBhIGRpc3Bvc2ljacOzbiBkZSBsb3MgdXN1YXJpb3MgYSB0cmF2w6lzIGRlbCByZXBvc2l0b3JpbyBpbnN0aXR1Y2lvbmFsIHBhcmEgcXVlIHNlIGhhZ2EgdW4gdXNvIGp1c3RvLCByZXNwZXRhbmRvIGxvcyBkZXJlY2hvcyBkZSBhdXRvciBxdWUgbGEgbGVnaXNsYWNpw7NuIGVzdGFibGVjZSB5IGNvbmZvcm1lIGNvbiBsYXMgY29uZGljaW9uZXMgbWFyY2FkYXMgcG9yIGxhIGxpY2VuY2lhIGRlIHVzbwo=
oai:idus.us.es:11441/1539952024-01-26T12:56:55Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
García Quintanilla, Meritxell de Jesús
Pulido, Marina R.
López Rojas, Rafael
Pachón Díaz, Jerónimo
McConnell, Michael J.
2024-01-25T12:10:52Z
2024-01-25T12:10:52Z
2013-03
2014-03
García Quintanilla, M.d.J., Pulido, M.R., López Rojas, R., Pachón Diaz, J. y McConnell, M.J. (2013). Emerging therapies for multidrug resistant Acinetobacter baumannii. Trends in Microbiology, 21 (3), 157-163. https://doi.org/10.1016/j.tim.2012.12.002.
0966-842X
1878-4380
https://hdl.handle.net/11441/153995
10.1016/j.tim.2012.12.002
The global emergence of multidrug resistant Acinetobacter baumannii has reduced the number of clinically available antibiotics that retain activity against this pathogen. For this reason, the development of novel prevention and treatment strategies for infections caused by A. baumannii is necessary. Several studies have begun to characterize nonantibiotic approaches that utilize novel mechanisms of action to achieve antibacterial activity. Recent advances in phage therapy, iron chelation therapy, antimicrobial peptides, prophylactic vaccination, photodynamic therapy, and nitric oxide (NO)-based therapies have all been shown to have activity against A. baumannii. However, before these approaches can be used clinically there are still limitations and remaining questions that must be addressed.
European Community 278232
European Development Regional Fund \'A way to achieve Europe\' ERDF
Ministerio de Economia y Competitividad of Spain
Ministerio de Economia y Competitividad, Instituto de Salud Carlos III
Spanish Network for the Research in Infectious Diseases REIPI RD06/0008/0000
application/pdf
7
eng
Cell Press
Trends in Microbiology, 21 (3), 157-163.
https://www.sciencedirect.com/science/article/pii/S0966842X12002259?via%3Dihub#aep-keywords-id12
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Acinetobacter baumannii
Antibiotic resistance
Phage therapy
Vaccine
Antimicrobial peptides
Iron chelation therapy
Emerging therapies for multidrug resistant Acinetobacter baumannii
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Medicina
Universidad de Sevilla. CTS-210: Resistencia a Antimicrobianos
Trends in Microbiology
21
3
157
163
ORIGINAL
2013_Emerging therapies for multidrug resistant Acinetobacter baumannii.pdf
2013_Emerging therapies for multidrug resistant Acinetobacter baumannii.pdf
application/pdf
2268612
https://idus.us.es/bitstream/11441/153995/1/2013_Emerging%20therapies%20for%20multidrug%20resistant%20Acinetobacter%20baumannii.pdf
2879561d8c79d6938b4b20ba7806820f
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1142
https://idus.us.es/bitstream/11441/153995/2/license.txt
4ccdb332745e121f04fdde82917fec0c
MD5
2
open access
11441/153995
oai:idus.us.es:11441/153995
2024-01-26 13:56:55.432
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/633422024-02-14T20:13:25Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Hernández, J. R.
Martínez Martínez, Luis
Cantón, Rafael
Coque, T. M.
Pascual Hernández, Álvaro
2017-07-27T11:41:18Z
2017-07-27T11:41:18Z
2005-05-01
Hernández, J.R., Martínez Martínez, L., Cantón, R., Coque, T.M. y Pascual Hernández, Á. (2005). Nationwide study of Escherichia coli and Klebsiella pneumoniae producing extended-spectrum β-lactamases in Spain. Antimicrobial Agents and Chemotherapy, 49 (5), 2122-2125.
0066-4804 (impreso)
1098-6596 (electrónico)
http://hdl.handle.net/11441/63342
10.1128/AAC.49.5.2122-2125.2005
Clonal dissemination of extended-spectrum -lactamases (ESBL) in 170 Escherichia coli isolates and 70 Klebsiella pneumoniae isolates from a nationwide study of 40 Spanish centers in 2000 was not observed in most
centers. The most prevalent ESBL were CTX-M-9 (27.3%), SHV-12 (23.9%), and CTX-M-14 (20.5%) for E. coli and TEM-3 (16.7%) and TEM-4 (25%) for K. pneumoniae. A new ESBL, TEM-133, with mutations L21F, E104K, and R164S, was identified.
application/pdf
eng
American Society for Microbiology
Antimicrobial Agents and Chemotherapy, 49 (5), 2122-2125.
http://dx.doi.org/10.1128/AAC.49.5.2122-2125.2005
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Nationwide study of Escherichia coli and Klebsiella pneumoniae producing extended-spectrum β-lactamases in Spain
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Antimicrobial Agents and Chemotherapy
49
5
2122
2125
https://ror.org/03yxnpp24
4 p.
ORIGINAL
Nationwide study of Escherichia coli and Klebsiella pneumoniae producing extended-spectrum β-lactamases in Spain.pdf
Nationwide study of Escherichia coli and Klebsiella pneumoniae producing extended-spectrum β-lactamases in Spain.pdf
application/pdf
114646
https://idus.us.es/bitstream/11441/63342/1/Nationwide%20study%20of%20Escherichia%20coli%20and%20Klebsiella%20pneumoniae%20producing%20extended-spectrum%20%c3%8e%c2%b2-lactamases%20in%20Spain.pdf
80630f944a2cfca97db9cd6952e8854d
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/63342/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/63342/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/63342
oai:idus.us.es:11441/63342
2024-02-14 21:13:25.615
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/650082024-02-15T07:21:42Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Pascual Hernández, Álvaro
García Luque, Isabel
Conejo Gonzalo, Mª Carmen
Perea Pérez, Evelio José
2017-10-04T16:50:55Z
2017-10-04T16:50:55Z
1993-01-01
Pascual Hernández, Á., García Luque, I., Conejo Gonzalo, M.C. y Perea Pérez, E.J. (1993). Uptake and intracellular activity of fluconazole in human polymorphonuclear leukocytes. Antimicrobial Agents and Chemotherapy, 37 (2), 187-190.
0066-4804 (impreso)
1098-6596 (electrónico)
http://hdl.handle.net/11441/65008
The penetration of fluconazole into human polymorphonuclear leukocytes (PMNs) and tissue culture epithelial cells (McCoy) was evaluated. At different extracellular concentrations (0.5 to 10 mg/liter), fluconazole reached cell-associated concentrations greater than the extracellular ones in either human PMNs (intracellular concentration to extracellular concentration ratio, ≥2.2) or McCoy cells (intracellular concentration to extracellular concentration ratio, ≥1.3). The uptake of fluconazole by PMNs was rapid and reversible but was not energy dependent. The intracellular penetration of fluconazole was not affected by environmental pH or temperature. Ingestion of opsonized zymosan and opsonized Candida albicans did not significantly increase the amount of PMN-associated fluconazole. At therapeutic extracellular concentrations, the intracellular activity of fluconazole against C. albicans in PMNs was significantly lower than that of amphotericin B. It was concluded that fluconazole reaches high intracellular concentrations within PMNs but shows moderate activity against intracellular C. albicans in vitro.
application/pdf
eng
American Society for Microbiology
Antimicrobial Agents and Chemotherapy, 37 (2), 187-190.
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Uptake and intracellular activity of fluconazole in human polymorphonuclear leukocytes
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Antimicrobial Agents and Chemotherapy
37
2
187
190
https://ror.org/03yxnpp24
4 p.
ORIGINAL
Uptake and intracellular activity of fluconazole in human polymorphonuclear leukocytes.pdf
Uptake and intracellular activity of fluconazole in human polymorphonuclear leukocytes.pdf
application/pdf
796820
https://idus.us.es/bitstream/11441/65008/1/Uptake%20and%20intracellular%20activity%20of%20fluconazole%20in%20human%20polymorphonuclear%20leukocytes.pdf
c723993811ff953d65936bd8d4e35881
MD5
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open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/65008/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/65008/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/65008
oai:idus.us.es:11441/65008
2024-02-15 08:21:42.587
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1375442024-02-14T09:25:48Zcom_11441_11024com_11441_10983com_11441_10690com_11441_11029com_11441_11044com_11441_10903com_11441_10802col_11441_11025col_11441_11030col_11441_11045col_11441_10904
Sojo Dorado, Jesús
López Hernández, Inmaculada
Rosso Fernández, Clara
Morales, Isabel
Palacios-Baena, Zaira R.
Hernández Torres, Alicia
Lobo Acosta, María Ángeles
Merino Bohórquez, Vicente
Pascual Hernández, Álvaro
Rodríguez-Baño, Jesús
Retamar Gentil, Pilar
2022-09-30T16:19:30Z
2022-09-30T16:19:30Z
2022
Sojo Dorado, J., López Hernández, ., Rosso Fernández, C., Morales, I., Palacios-Baena, Z.R., Hernández Torres, A.,...,Rodríguez-Baño, J. (2022). Effectiveness of Fosfomycin for the Treatment of Multidrug-Resistant Escherichia coli Bacteremic Urinary Tract Infections A Randomized Clinical Trial. Jama Network Open, 5 (1), 115-14.
2574-3805
https://hdl.handle.net/11441/137544
10.1001/jamanetworkopen.2021.37277
Importance The consumption of broad-spectrum drugs has increased as a consequence of the spread of multidrug-resistant (MDR) Escherichia coli. Finding alternatives for these infections is critical, for which some neglected drugs may be an option.
Objective To determine whether fosfomycin is noninferior to ceftriaxone or meropenem in the targeted treatment of bacteremic urinary tract infections (bUTIs) due to MDR E coli.
Design, Setting, and Participants This multicenter, randomized, pragmatic, open clinical trial was conducted at 22 Spanish hospitals from June 2014 to December 2018. Eligible participants were adult patients with bacteremic urinary tract infections due to MDR E coli; 161 of 1578 screened patients were randomized and followed up for 60 days. Data were analyzed in May 2021.
Interventions Patients were randomized 1 to 1 to receive intravenous fosfomycin disodium at 4 g every 6 hours (70 participants) or a comparator (ceftriaxone or meropenem if resistant; 73 participants) with the option to switch to oral fosfomycin trometamol for the fosfomycin group or an active oral drug or parenteral ertapenem for the comparator group after 4 days.
Main Outcomes and Measures The primary outcome was clinical and microbiological cure (CMC) 5 to 7 days after finalization of treatment; a noninferiority margin of 7% was considered.
Results Among 143 patients in the modified intention-to-treat population (median [IQR] age, 72 [62-81] years; 73 [51.0%] women), 48 of 70 patients (68.6%) treated with fosfomycin and 57 of 73 patients (78.1%) treated with comparators reached CMC (risk difference, −9.4 percentage points; 1-sided 95% CI, −21.5 to ∞ percentage points; P = .10). While clinical or microbiological failure occurred among 10 patients (14.3%) treated with fosfomycin and 14 patients (19.7%) treated with comparators (risk difference, −5.4 percentage points; 1-sided 95% CI, −∞ to 4.9; percentage points; P = .19), an increased rate of adverse event–related discontinuations occurred with fosfomycin vs comparators (6 discontinuations [8.5%] vs 0 discontinuations; P = .006). In an exploratory analysis among a subset of 38 patients who underwent rectal colonization studies, patients treated with fosfomycin acquired a new ceftriaxone-resistant or meropenem-resistant gram-negative bacteria at a decreased rate compared with patients treated with comparators (0 of 21 patients vs 4 of 17 patients [23.5%]; 1-sided P = .01).
Conclusions and Relevance This study found that fosfomycin did not demonstrate noninferiority to comparators as targeted treatment of bUTI from MDR E coli; this was due to an increased rate of adverse event–related discontinuations. This finding suggests that fosfomycin may be considered for selected patients with these infections.
application/pdf
14 p.
eng
Jama Nerwork Open
Jama Network Open, 5 (1), 115-14.
https://jamanetwork.com/journals/jamanetworkopen/fullarticle/2788111
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Fosfomycin
Urinary Tract Infections
Escherichia coli
Effectiveness of Fosfomycin for the Treatment of Multidrug-Resistant Escherichia coli Bacteremic Urinary Tract Infections A Randomized Clinical Trial
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Farmacología, Pediatría y Radiología
Universidad de Sevilla. Departamento de Farmacología
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Medicina
Jama Network Open
5
1
115
14
https://ror.org/03yxnpp24
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/137544/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
ORIGINAL
Effectiveness of Fosfomycin.pdf
Effectiveness of Fosfomycin.pdf
application/pdf
1014068
https://idus.us.es/bitstream/11441/137544/1/Effectiveness%20of%20Fosfomycin.pdf
b87882cb93170f57535cea238bbd8197
MD5
1
open access
11441/137544
oai:idus.us.es:11441/137544
2024-02-14 10:25:48.807
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/891992019-09-19T08:43:46Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
López Baena, Francisco Javier
Vinardell González, José María
Medina Morillas, Carlos
2019-09-19T08:43:46Z
2019-09-19T08:43:46Z
2019-06-12
López Baena, F.J., Vinardell González, J.M. y Medina Morillas, C. (2019). Regulation of Protein Secretion Systems Mediated by Cyclic Diguanylate in Plant-Interacting Bacteria. Frontiers in Microbiology, 10, 1289-1-1289-9.
1664-302X
https://hdl.handle.net/11441/89199
10.3389/fmicb.2019.01289
The ubiquitous second messenger cyclic diguanylate (c-di-GMP) is involved in the regulation of different processes in bacteria. In phytopathogens, intracellular fluctuations in the concentration of this molecule contribute to the lifestyle switching from a motile and virulent stage to a sessile and biofilm-forming phase. Among the virulence mechanisms used by bacterial pathogens, different specific type secretion systems (TSSs) and the effector proteins that they translocate are included. Some of these TSS are conceived to suppress host immune responses during bacterial colonization. The modulation of the expression of secretion systems components and/or effector proteins can be influenced by c-di-GMP levels at transcriptional, translational, or post-translational levels and can take place directly by binding to specific or global regulators, or via transducer proteins. Different genera of plant-interacting bacteria have been analyzed to shed some light in the implications of c-di-GMP in the regulation of host plant colonization through protein secretion systems. Expression of (1) adhesins secreted by Type 1 secretion systems to bind the host plant in Pectobacterium (formerly Erwinia) and some beneficial Pseudomonas strains; (2) catalytic exoproteins delivered by Type 2 secretion systems to break plant cell wall in Dickeya; (3) effectors secreted by Type 3 secretion systems to suppress plant immunity in Xanthomonas; or (4) the activity of Type 6 secretion systems to export an ATPase in Pseudomonas, are finely tuned by c-di-GMP levels. In this minireview, we summarize the knowledge available about the implications of c-di-GMP in the regulation of protein secretion in different plant-interacting bacteria.
Spanish Ministry of Economy, Industry and Competitivity BIO2016-78409-R
application/pdf
eng
Frontiers Research Foundation
Frontiers in Microbiology, 10, 1289-1-1289-9.
BIO2016-78409-R
https://doi.org/10.3389/fmicb.2019.01289
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Cyclic diguanylate
Protein secretion systems
Diguanylate cyclase
Phosphodiesterase
Adhesins
Extracellular degradative enzymes
Effector proteins
ATPase
Regulation of Protein Secretion Systems Mediated by Cyclic Diguanylate in Plant-Interacting Bacteria
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Frontiers in Microbiology
10
1289-1
1289-9
9 p.
ORIGINAL
Regulation of Protein Secretion Systems.pdf
Regulation of Protein Secretion Systems.pdf
application/pdf
706414
https://idus.us.es/bitstream/11441/89199/1/Regulation%20of%20Protein%20Secretion%20Systems.pdf
d70b90b1d8581fe857114aaec58db057
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/89199/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/89199
oai:idus.us.es:11441/89199
2019-09-19 10:43:46.856
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/233482024-02-13T22:28:36Zcom_11441_10903com_11441_10802com_11441_10690com_11441_11084com_11441_10983com_11441_10923col_11441_10904col_11441_11085col_11441_10924
Gil Serrano, Antonio Miguel
Rodríguez Carvajal, Miguel Ángel
Tejero Mateo, María Pilar
Espartero Sánchez, José Luis
Thomas Oates, J.
Ruiz Sainz, José Enrique
Buendía Clavería, Ana María
2015-03-03T13:35:24Z
2015-03-03T13:35:24Z
1998
1932-6203
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1219727/pdf/9729466.pdf
http://hdl.handle.net/11441/23348
https://idus.us.es/xmlui/handle/11441/23348
eng
PLoS ONE, 334, 585-594
Atribución-NoComercial-SinDerivadas 4.0 España
http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
Structural determination of a 5-o-methyl-deaminated neuraminic acid (Kdn)-containing polysaccharide isolated from Sinorhizobium fredii
info:eu-repo/semantics/article
Universidad de Sevilla. Departamento de Química Orgánica
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica
https://ror.org/03yxnpp24
ORIGINAL
file_1.pdf
application/pdf
1097048
https://idus.us.es/bitstream/11441/23348/1/file_1.pdf
3ba4eb9cb95fe4873f8b6d2cfe297742
MD5
1
open access
11441/23348
oai:idus.us.es:11441/23348
2024-02-13 23:28:36.752
open access
idUS - Universidad de Sevilla
idus@us.es
oai:idus.us.es:11441/1108462024-02-14T19:31:51Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Egea, Viviana de
Muñoz, Patricia
Valerio, Maricela
Alarcón, Arístides de
Lepe Jiménez, José Antonio
Miró, José M.
Gálvez-Acebal, Juan
García-Pavía, Pablo
Navas, Enrique
2021-05-26T11:55:27Z
2021-05-26T11:55:27Z
2015
Egea, V.d., Muñoz, P., Valerio, M., Alarcón, A.d., Lepe Jiménez, J.A., Miró, J.M.,...,Navas, E. (2015). Characteristics and Outcome of Streptococcus pneumoniae Endocarditis in the XXI Century. A Systematic Review of 111 Cases (2000–2013). Medicine, 94 (39), 1-11.
1536-5964
https://hdl.handle.net/11441/110846
10.1097/md.0000000000001562
Streptococcus pneumoniae is an infrequent cause of severe infectious endocarditis (IE). The aim of our study was to describe the epidemiology, clinical and microbiological characteristics, and outcome of a series of cases of S. pneumoniae IE diagnosed in Spain and in a series of cases published since 2000 in the medical literature. We prospectively collected all cases of IE diagnosed in a multicenter cohort of patients from 27 Spanish hospitals (n¼2539). We also performed a systematic review of the literature since 2000 and retrieved all cases with complete clinical data using a pre-established protocol.
Predictors of mortality were identified using a logistic regression model. We collected 111 cases of pneumococcal IE: 24 patients from the Spanish cohort and 87 cases from the literature review. Median age was 51 years, and 23 patients (20.7%) were under 15 years. Men accounted for 64% of patients, and infection was community-acquired in 96.4% of
cases. The most important underlying conditions were liver disease (27.9%) and immunosuppression (10.8%). A predisposing heart condition was present in only 18 patients (16.2%). Pneumococcal IE affected a native valve in 93.7% of patients. Left-sided endocarditis predominated (aortic valve 53.2% and mitral valve 40.5%). The microbiological diagnosis was obtained from blood cultures in 84.7% of cases. In the Spanish cohort, nonsusceptibility to penicillin was detected in 4.2%. The most common clinical manifestations included fever (71.2%), a new heart murmur (55%), pneumonia (45.9%), meningitis (40.5%), and Austrian syndrome (26.1%). Cardiac surgery was performed in 47.7% of patients. The in-hospital mortality rate was 20.7%. The multivariate analysis revealed the independent risk factors for mortality to be meningitis (OR, 4.3; 95% CI, 1.4–12.9; P<0.01). Valve surgery was protective (OR, 0.1; 95% CI, 0.04–0.4; P<0.01).
Streptococcus pneumoniae IE is a community-acquired disease that mainly affects native aortic valves. Half of the cases in the present study had concomitant pneumonia, and a considerable number developed meningitis. Mortality was high, mainly in patients with central nervous system (CNS) involvement. Surgery was protective.
application/pdf
11
eng
Wolters Kluwer
Medicine, 94 (39), 1-11.
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Streptococcus pneumoniae
Endocarditis
Medical literature
Characteristics and Outcome of Streptococcus pneumoniae Endocarditis in the XXI Century. A Systematic Review of 111 Cases (2000–2013)
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. CTS204: Biotecnología Aplicada al Estudio de Enf. Infecciosas
Medicine
94
39
1
11
https://ror.org/03yxnpp24
ORIGINAL
Characteristics and Outcome of Streptococcus pneumoniae Endocarditis in the XXI Century.pdf
Characteristics and Outcome of Streptococcus pneumoniae Endocarditis in the XXI Century.pdf
application/pdf
252152
https://idus.us.es/bitstream/11441/110846/1/Characteristics%20and%20Outcome%20of%20Streptococcus%20pneumoniae%20Endocarditis%20in%20the%20XXI%20Century.pdf
1ef1f23aae5536a60055c22ca9fc329e
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/110846/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/110846
oai:idus.us.es:11441/110846
2024-02-14 20:31:51.175
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1371502024-02-14T19:10:43Zcom_11441_11044com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11045col_11441_10904
Salamanca Rivera, Elena
López Cerero, Lorena
Rodríguez Martínez, José Manuel
Pascual Hernández, Álvaro
Rodríguez-Baño, Jesús
2022-09-16T13:52:06Z
2022-09-16T13:52:06Z
2022
Salamanca Rivera, E., López Cerero, L., Rodríguez Martínez, J.M., Pascual Hernández, Á. y Rodríguez-Baño, J. (2022). Prevalence, Incidence, and Risk Factors for Intestinal Colonization Due to Fluoroquinolone-Resistant ST131 Escherichia coli: a Longitudinal Study in Highly Dependent, Long-Term Care Facility Residents. Microbiology spectrum, 10 (4), e0167322.
2165-0497
https://hdl.handle.net/11441/137150
10.1128/spectrum.01673-22
Escherichia coli ST131 clade C is an important driver for fluoroquinolone resistance (FQ-R). We conducted a prospective observational study in residents from two long-term care facilities (LTCFs) in Seville, Spain, in 2018. Fecal swabs and environmental samples were obtained. E. coli isolates were screened for clade C, FQ-R ST131 by PCR, and molecular typing by PFGE; representatives from pulsotypes were studied by whole-genome-sequencing (WGS) and assigned to lineages (cgSTs). Prevalence of colonization at each time point, incidence density, and risk factors for acquisition were studied. Seventy-six FQ-R ST131 E. coli isolates belonging to 34 cgSTs were obtained; 24 belonging to subclade C1 (116 isolates, 65.9%) and 10 to C2 (60, 34.1%). C1 lineages showed lower virulence scores than C2 (median [IQR], 19 [18 to 20] versus 21 [20 to 21.5], P = 0.001) and higher number of plasmids (4 [3 to 5] versus 2 [2 to 3], P = 0.01). aac(6')-Ib-cr and blaOXA-1 were less frequent in C1 than C2 (2 [8.3%] versus 6 [60%], P = 0.003 for both); ESBL genes were detected in eight (33.3%) C1 (5 blaCTX-M-27) and three (30%) C2 (all blaCTX-M-15). Of the 82 residents studied, 49 were colonized at some point (59.7%), with a pooled prevalence of 38.6%. Incidence density of new lineage acquisition was 2.22 per 100 resident weeks (1.28 and 0.93 C1 and C2 subclades, respectively). Independent risk factors for acquisitions were having a colonized roommate (HR = 4.21; 95% CI = 1.71 to 10.36; P = 0.002) and urinary or fecal incontinence (HR = 2.82; 95% CI = 1.21 to 6.56; P = 0.01). LTCFs are important reservoirs of clade C ST131 E. coli. The risk factors found suggest that cross-transmission is the most relevant transmission mechanisms. IMPORTANCE We aimed at investigating the microbiological and epidemiological features of clade C fluoroquinolone-resistant ST131 E. coli isolates colonizing highly dependent residents in long-term care facilities (LTCFs) during 40 weeks and the risk factors of acquisition. Isolates from C1 and C2 subclades were characterized in this environment. The clonality of the isolates was characterized and they were assigned to lineages (cgSTs), Resistance genes, virulence factors, and plasmids were also described. This study suggests that cross-transmission is the most relevant transmission mechanisms; however, environmental colonization might also play a role. We believe the data provide useful information to depict the epidemiology of these bacteria by merging detailed microbiological and epidemiological information.
Ministerio de Ciencia, Innovación y Universidades AC16/00072, AC16/00076, RD16/0016/0001
application/pdf
12 p.
eng
American Society for Microbiology
Microbiology spectrum, 10 (4), e0167322.
AC16/00072
AC16/00076
RD16/0016/0001
https://doi.org/10.1128/spectrum.01673-22
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Escherichia coli
Fluorquinolone resistance
Multidrug resistance
Risk factors
ST131
Prevalence, Incidence, and Risk Factors for Intestinal Colonization Due to Fluoroquinolone-Resistant ST131 Escherichia coli: a Longitudinal Study in Highly Dependent, Long-Term Care Facility Residents
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Medicina
Ministerio de Ciencia, Innovación y Universidades (MICINN). España
Microbiology spectrum
10
4
e0167322
https://ror.org/03yxnpp24
ORIGINAL
Prevalence Incidence and Risk.pdf
Prevalence Incidence and Risk.pdf
application/pdf
775056
https://idus.us.es/bitstream/11441/137150/1/Prevalence%20Incidence%20and%20Risk.pdf
693b013987ef723e84025ffeca122bde
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/137150/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/137150
oai:idus.us.es:11441/137150
2024-02-14 20:10:43.397
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/648542024-02-13T22:23:10Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Conejo Gonzalo, Mª Carmen
Pascual Hernández, Álvaro
Otero, Jesús
Ortega, Adriana
Bartolomé, Rosa M.
Bou, Germán
Fernández Martínez, Marta
González López, Juan José
2017-09-28T16:41:12Z
2017-09-28T16:41:12Z
2015-06-01
Conejo Gonzalo, M.C., Pascual Hernández, Á., Otero, J., Ortega, A., Bartolomé, R.M., Bou, G.,...,González López, J.J. (2015). Prospective multicenter study of carbapenemase-producing Enterobacteriaceae from 83 hospitals in Spain reveals high in vitro susceptibility to colistin and meropenem. Antimicrobial Agents and Chemotherapy, 59 (6), 3406-3412.
0066-4804 (impreso)
1098-6596 (electrónico)
http://hdl.handle.net/11441/64854
10.1128/AAC.00086-15
The aim of this study was to determine the impact of carbapenemase-producing Enterobacteriaceae (CPE) in Spain in 2013 by describing the prevalence, dissemination, and geographic distribution of CPE clones, and their population structure and antibiotic susceptibility. From February 2013 to May 2013, 83 hospitals (about 40,000 hospital beds) prospectively collected nonduplicate Enterobacteriaceae using the screening cutoff recommended by EUCAST. Carbapenemase characterization was performed by phenotypic methods and confirmed by PCR and sequencing. Multilocus sequencing types (MLST) were determined for Klebsiella pneumoniae and Escherichia coli. A total of 702 Enterobacteriaceae isolates met the inclusion criteria; 379 (54%) were CPE. OXA-48 (71.5%) and VIM-1 (25.3%) were the most frequent carbapenemases, and K. pneumoniae (74.4%), Enterobacter cloacae (10.3%), and E. coli (8.4%) were the species most affected. Susceptibility to colistin, amikacin, and meropenem was 95.5%, 81.3%, and 74.7%, respectively. The most prevalent sequence types (STs) were ST11 and ST405 for K. pneumoniae and ST131 for E. coli. Forty-five (54.1%) of the hospitals had at least one CPE case. For K. pneumoniae, ST11/OXA-48, ST15/OXA-48, ST405/OXA-48, and ST11/VIM-1 were detected in two or more Spanish provinces. ST11 isolates carried four carbapenemases (VIM-1, OXA-48, KPC-2, and OXA-245), but ST405 isolates carried OXA-48 only. A wide interregional spread of CPE in Spain was observed, mainly due to a few successful clones of OXA-48-producing K. pneumoniae (e.g., ST11 and ST405). The dissemination of OXA-48-producing E. coli is a new finding of public health concern. According to the susceptibilities determined in vitro, most of the CPE (94.5%) had three or more options for antibiotic treatment
Fondo de Investigación Sanitaria PI12/01242
General de Redes y Centros de Investigación Cooperativa y Ministerio de Economía y Competitividad y Spanish Network for Research in Infectious Diseases REIPI RD12/0015
application/pdf
eng
American Society for Microbiology
Antimicrobial Agents and Chemotherapy, 59 (6), 3406-3412.
PI12/01242
info:eu-repo/grantAgreement/MINECO/RD12/0015
http://dx.doi.org/10.1128/AAC.00086-15
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Prospective multicenter study of carbapenemase-producing Enterobacteriaceae from 83 hospitals in Spain reveals high in vitro susceptibility to colistin and meropenem
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Instituto de Salud Carlos III
Ministerio de Economía y Competitividad (MINECO). España
Antimicrobial Agents and Chemotherapy
59
6
3406
3412
https://ror.org/03yxnpp24
7 p.
ORIGINAL
Prospective multicenter study of carbapenemase-producing Enterobacteriaceae from 83 hospitals in Spain reveals high in vitro susceptibility to colistin and meropenem.pdf
Prospective multicenter study of carbapenemase-producing Enterobacteriaceae from 83 hospitals in Spain reveals high in vitro susceptibility to colistin and meropenem.pdf
application/pdf
824388
https://idus.us.es/bitstream/11441/64854/1/Prospective%20multicenter%20study%20of%20carbapenemase-producing%20Enterobacteriaceae%20from%2083%20hospitals%20in%20Spain%20reveals%20high%20in%20vitro%20susceptibility%20to%20colistin%20and%20meropenem.pdf
57a2f5d9fd22c95b30589610574ae153
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/64854/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/64854/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/64854
oai:idus.us.es:11441/64854
2024-02-13 23:23:10.69
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/650032024-02-12T21:38:26Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Pascual Hernández, Álvaro
Joyanes, Providencia
Martínez Martínez, Luis
Suárez, Ana Isabel
Perea Pérez, Evelio José
2017-10-04T16:43:53Z
2017-10-04T16:43:53Z
1996
Pascual Hernández, Á., Joyanes, P., Martínez Martínez, L., Suárez, A.I. y Perea Pérez, E.J. (1996). Comparison of broth microdilution and E-test for susceptibility testing of Neisseria meningitidis. Journal of Clinical Microbiology, 34 (3), 588-591.
0095-1137 (impreso)
1098-660X (electronico)
http://hdl.handle.net/11441/65003
The susceptibilities of 54 clinical isolates of Neisseria meningitidis to penicillin, cefotaxime, ceftriaxone, cefepime, imipenem, ciprofloxacin, chloramphenicol, and rifampin were determined by the microdilution method in both cation-adjusted Mueller-Hinton broth (CAMHB) and Haemophilus test medium (HTM). Poor growth was observed in 16.6 and 9% of the strains in CAMHB and HTM, respectively. As a result, the growth of the 54 N. meningitidis strains was evaluated in three other commercially available hatches of CAMHB and in one in-house batch of HTM. Poor growth was observed for 9.3 to 16.6% of the strains in all four batches. More important, three of the CAMHB batches failed to support growth for 3.7 to 33.3% of the strains; 3.7% of the strains did not grow in the in-house-prepared HTM. Ten (18.7%) strains were relatively resistant to penicillin (RRP; MIC, >0.125 μg/ml) in CAMHB and 13 (24%) strains were RRP in HTM. The percentages of agreement obtained by using CAMHB as the reference ranged from 78% for cefepime to 100% for ceftriaxone. Seven minor errors were observed for penicillin; five of them were for strains susceptible to penicillin in CAMHB and RRP in HTM. All strains were susceptible to the other antimicrobial agents evaluated. The growth of N. meningitidis was also evaluated in four batches of Mueller-Hinton agar (MHA). In two of them, 3.7 and 44.4% of the strains did not grow, and considering all four batches, 5.5 to 11.1% grew poorly. All strains grew adequately in MHA supplemented with blood (MHA-b). The activities of penicillin and cefotaxime were also evaluated by the E-test in MHA and MHA-b. The proportion of RRP strains were 24% in MHA and 59% in MHA-b. For penicillin, the percentages of agreement of the E-test with the microdilution method in CAMHB (reference) were 64.8 and 70.3% in MHA and MHA-b, respectively. For cefotaxime, the agreement was 98.1%. Minor errors for the penicillin MIC were detected for 38% of the strains tested. Further studies are needed to define adequate culture media for reference methods to evaluate the susceptibility of N. meningitidis to antimicrobial agents.
application/pdf
eng
American Society for Microbiology
Journal of Clinical Microbiology, 34 (3), 588-591.
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Comparison of broth microdilution and E-test for susceptibility testing of Neisseria meningitidis
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Journal of Clinical Microbiology
34
3
588
591
https://ror.org/03yxnpp24
4 p.
ORIGINAL
Comparison of broth.pdf
Comparison of broth.pdf
application/pdf
170984
https://idus.us.es/bitstream/11441/65003/1/Comparison%20of%20broth.pdf
3e01623a4c19553e30d2523f00a4a7e7
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/65003/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/65003/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/65003
oai:idus.us.es:11441/65003
2024-02-12 22:38:26.44
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/367182024-02-14T20:08:03Zcom_11441_11014com_11441_10983com_11441_10690com_11441_11049com_11441_10903com_11441_10802col_11441_11015col_11441_11050col_11441_10904
Ríos-Santos, J.V.
Borobio Enciso, María Victoria
Velasco-Ortega, Eugenio
Martínez-Sahuquillo Márquez, Ángel
Machuca-Portillo, Guillermo
Bullon, Pedro
2016-03-02T10:09:36Z
2016-03-02T10:09:36Z
1995
0213-4144
http://hdl.handle.net/11441/36718
https://idus.us.es/xmlui/handle/11441/36718
Se realiza un seguimiento clínico y microbiológico durante dos años de 20 pacientes (2228 zonas) con periodontitis crónica del adulto evaluando la respuesta al raspado y alisado radicular. Se observa una recuperación de inserción al finalizar el raspado, que se pierde en parte a los dos años. Un análisis discriminante demostró que el sangrado es un buen factor de agrupación de las bolsas, encontrándose correlacionado con placa y nivel de inserción. Un análisis de regresión múltiple estimó que cuando la bolsa sangraba al comienzo del estudio, la respuesta clínica satisfactoria se reducía al 50%. Se observó una reducción de la flora patógena a los seis meses del raspado con reinstalación de los periodontopatógenos a los dos años. Además, los pacientes con evolución clínica desfavorable tenían mayor concentración al comienzo del estudio de bacilos gram negativos anaerobios y facultativos que los pacientes con evolución favorable.
The present longitudinal study was conducted during two years in 20 patients (2228 sites) suffering from adult chronic periodontitis to evaluate clinical and microbiological changes. It was observed a gaining in clinical attachment after of scaling an root planning, that was lost after of scaling an root planning, that was lost after 2 years. Discriminant analysis showed that bleeding on probing is a good factor to groupe the pockets and was relate to plaque and clinical attachment loss. A multiple regression analysis demonstrated that when the pocket was bleeding at first visit good clinical response was reduced in 50%. At six months the microbiological flora was reduce, but at two years periodontal pathogens reappeared. The patients with worst clinical results had at the first visit more gram negative anaerobe and facultative bacteria than patients with good clinical evolution.
application/pdf
spa
Ergon
Archivos de odontoestomatología, 11 (1), 1-13
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Microbiología oral
Periodontitis crónica
Raspado y alisado
Estudio longitudinal
Microbial flora
Chronic periodontitis
Scaling and root planning
Longitudinal studies
Estudio longitudinal (2 años) de la periodontitis crónica del adulto: respuesta clínica y microbiológica
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Estomatología
https://ror.org/03yxnpp24
ORIGINAL
Estudio_longitudinal_de_la_periodontitis_crónica_del_adulto_respuesta_clínica_y_microbiológica.pdf
Estudio_longitudinal_de_la_periodontitis_crónica_del_adulto_respuesta_clínica_y_microbiológica.pdf
application/pdf
2017354
https://idus.us.es/bitstream/11441/36718/1/Estudio_longitudinal_de_la_periodontitis_cr%c3%b3nica_del_adulto_respuesta_cl%c3%adnica_y_microbiol%c3%b3gica.pdf
431180ec82a800c1e1d73ccc7d69278e
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/36718/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/36718/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/36718
oai:idus.us.es:11441/36718
2024-02-14 21:08:03.258
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1478112023-07-07T14:30:34Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Ramos, Juan Luis
Bernal Guzmán, Patricia
Salvachua, Davinia
2023-07-07T14:30:34Z
2023-07-07T14:30:34Z
2023
Ramos, J.L., Bernal Guzmán, P. y Salvachua, D. (2023). Microbial biotechnology in the effort to end hunger. Microbial Biotechnology. https://doi.org/10.1111/1751-7915.14295.
1751-7907
1751-7915
https://hdl.handle.net/11441/147811
10.1111/1751-7915.14295
Comisión Interministerial de Ciencia y Tecnología PID20211234690BI00
application/pdf
3 p.
eng
Wiley-Blackwell
Microbial Biotechnology.
PID20211234690BI00
https://doi.org/10.1111/1751-7915.14295
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Microbial biotechnology in the effort to end hunger
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Comisión Interministerial de Ciencia y Tecnología (CICYT). España
Microbial Biotechnology
ORIGINAL
Microbial biotechnology.pdf
Microbial biotechnology.pdf
application/pdf
302590
https://idus.us.es/bitstream/11441/147811/1/Microbial%20biotechnology.pdf
0a183d980af19f793d93fd802d594de4
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1142
https://idus.us.es/bitstream/11441/147811/2/license.txt
4ccdb332745e121f04fdde82917fec0c
MD5
2
open access
11441/147811
oai:idus.us.es:11441/147811
2023-07-07 16:30:34.727
open access
idUS - Universidad de Sevilla
idus@us.es
RGVjbGFyYSBxdWU6CgphKSBFcyBhdXRvciB5IHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIHByb3BpZWRhZCBpbnRlbGVjdHVhbC4KCgpiKSBTaSBleGlzdGUgcHJldmlhIGNlc2nDs24gYSB0ZXJjZXJvcyBkZSBsb3MgZGVyZWNob3MgZGUgZXhwbG90YWNpw7NuIGRlIGxhIG9icmEsIGN1ZW50YSBjb24gbGEgYXV0b3JpemFjacOzbiBkZSBkaWNob3MgdGl0dWxhcmVzLiAKCgpjKSBTaSBlbCBkb2N1bWVudG8gY29udGllbmUgbWF0ZXJpYWxlcyBkZSBsb3MgcXVlIG5vIGVzIHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIGV4cGxvdGFjacOzbiwgdGllbmUgZWwgcGVybWlzbyBwYXJhIGRlcG9zaXRhcmxvcyB5IHF1ZSBlc2UgbWF0ZXJpYWwgZXN0w6EgaWRlbnRpZmljYWRvIGNsYXJhbWVudGUuCgoKRWwgYXV0b3IgcmVhbGl6YSBsYSBjZXNpw7NuIGdyYXR1aXRhIHkgbm8gZXhjbHVzaXZhIGEgbGEgVW5pdmVyc2lkYWQgZGUgU2V2aWxsYSBkZSBsb3MgZGVyZWNob3MgZGUgcmVwcm9kdWNjacOzbiwgZGlzdHJpYnVjacOzbiwgY29tdW5pY2FjacOzbiBww7pibGljYSB5IHRyYW5zZm9ybWFjacOzbjsgcGVybWl0aWVuZG8gYWwgcmVwb3NpdG9yaW86CgoKCSogVHJhbnNmb3JtYWNpw7NuIGRlbCBmb3JtYXRvIHBhcmEgc3UgaW5jb3Jwb3JhY2nDs24geSBwcmVzZXJ2YWNpw7NuLgoKCgkqIFJlcHJvZHVjY2nDs24geSBhcmNoaXZvIGVuIGxvcyBzZXJ2aWRvcmVzIGFzb2NpYWRvcyBhbCByZXBvc2l0b3Jpby4KCgoJKiBTdSBjb211bmljYWNpw7NuIHDDumJsaWNhIHkgc3UgcHVlc3RhIGEgZGlzcG9zaWNpw7NuIGRlIG1vZG8gbGlicmUgeSBncmF0dWl0byBhIHRyYXbDqXMgZGVsIGFyY2hpdm8gYWJpZXJ0byBpbnN0aXR1Y2lvbmFsLgoKCkVsIGF1dG9yIGF1dG9yaXphIHF1ZSBsYSBvYnJhIHNlIHBvbmdhIGEgZGlzcG9zaWNpw7NuIGRlIGxvcyB1c3VhcmlvcyBhIHRyYXbDqXMgZGVsIHJlcG9zaXRvcmlvIGluc3RpdHVjaW9uYWwgcGFyYSBxdWUgc2UgaGFnYSB1biB1c28ganVzdG8sIHJlc3BldGFuZG8gbG9zIGRlcmVjaG9zIGRlIGF1dG9yIHF1ZSBsYSBsZWdpc2xhY2nDs24gZXN0YWJsZWNlIHkgY29uZm9ybWUgY29uIGxhcyBjb25kaWNpb25lcyBtYXJjYWRhcyBwb3IgbGEgbGljZW5jaWEgZGUgdXNvLgo=
oai:idus.us.es:11441/231442024-02-12T21:32:17Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Negro, Juan José
Gortázar Schmidt, Ramón Christian
Torres Sánchez, María José
Vicente, Joaquín
Acevedo, Pelayo
Reglero, Manuel
Fuente, José de la
Aznar Martín, Javier
2015-02-27T12:21:09Z
2015-02-27T12:21:09Z
2008
Negro, J.J., Gortázar Schmidt, R.C., Torres Sánchez, M.J., Vicente, J., Acevedo, P., Reglero, M.,...,Aznar Martín, J. (2008). Bovine Tuberculosis in Doñana Biosphere Reserve: The Role of Wild Ungulates as Disease Reservoirs in the Last Iberian Lynx Strongholds.
1932-6203
http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0002776&representation=PDF
http://hdl.handle.net/11441/23144
https://idus.us.es/xmlui/handle/11441/23144
eng
Atribución-NoComercial-SinDerivadas 4.0 España
http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
Bovine Tuberculosis in Doñana Biosphere Reserve: The Role of Wild Ungulates as Disease Reservoirs in the Last Iberian Lynx Strongholds
info:eu-repo/semantics/article
Universidad de Sevilla. Departamento de Microbiología
https://ror.org/03yxnpp24
ORIGINAL
file_1.pdf
application/pdf
202418
https://idus.us.es/bitstream/11441/23144/1/file_1.pdf
8b2f413b6f2f037ba4274bc51d51d338
MD5
1
open access
11441/23144
oai:idus.us.es:11441/23144
2024-02-12 22:32:17.847
open access
idUS - Universidad de Sevilla
idus@us.es
oai:idus.us.es:11441/662672024-02-14T19:05:24Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Megías, Esaú
Reis Junior, Fábio Bueno
Ribeiro, Renan Augusto
Ollero Márquez, Francisco Javier
Megías Guijo, Manuel
Hungria, Mariangela
2017-11-20T16:11:16Z
2017-11-20T16:11:16Z
2017
Megías, E., Reis Junior, F.B., Ribeiro, R.A., Ollero Márquez, F.J., Megías Guijo, M. y Hungria, M. (2017). Genome Sequence of Pantoea ananatis Strain AMG 501, a Plant Growth-Promoting Bacterium Isolated from Rice Leaves Grown in Paddies of Southern Spain. Genome Announcements, 5 (34), e00848-1-e00848-17.
2169-8287
http://hdl.handle.net/11441/66267
10.1128/genomeA.00848-17
Pantoea ananatis AMG 501 is a plant growth-promoting bacterium isolated from rice leaves. Its genome was estimated at 5,102,640 bp with 4,994 coding sequences, encompassing genes related to the metabolism of carbohydrates, to the synthesis of auxins, siderophores, and homoserine lactones, and to the type I, II, III, IV, and VI secretion systems.
España, MINECO AGL2016-77163-R
application/pdf
eng
American Society for Microbiology
Genome Announcements, 5 (34), e00848-1-e00848-17.
AGL2016-77163-R
http://dx.doi.org/10.1128/genomeA.00848-17
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Genome Sequence of Pantoea ananatis Strain AMG 501, a Plant Growth-Promoting Bacterium Isolated from Rice Leaves Grown in Paddies of Southern Spain
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Ministerio de Economía y Competitividad (MINECO). España
Genome Announcements
5
34
e00848-1
e00848-17
https://ror.org/03yxnpp24
18 p.
ORIGINAL
pub3e00848-17.pdf
pub3e00848-17.pdf
application/pdf
126327
https://idus.us.es/bitstream/11441/66267/1/pub3e00848-17.pdf
85054386fd44cf5c2753f037ad7ddb53
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/66267/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/66267/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/66267
oai:idus.us.es:11441/66267
2024-02-14 20:05:24.039
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1442512024-02-14T13:28:21Zcom_11441_11044com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11045col_11441_10904
Almenara-Tejederas, Marina
Rodríguez-Pérez, María A.
Moyano-Franco, María J.
Cueto López, Marina de
Rodríguez-Baño, Jesús
Salgueira Lazo, Mercedes
2023-04-12T13:32:13Z
2023-04-12T13:32:13Z
2022-08-17
Almenara-Tejederas, M., Rodríguez-Pérez, M.A., Moyano-Franco, M.J., Cueto López, M.d., Rodríguez-Baño, J. y Salgueira Lazo, M. (2022). Tunneled catheter-related bacteremia in hemodialysis patients: incidence, risk factors and outcomes. A 14-year observational study. JOURNAL OF NEPHROLOGY, 36 (1), 203-212. https://doi.org/10.1007/s40620-022-01408-8.
1121-8428
https://hdl.handle.net/11441/144251
10.1007/s40620-022-01408-8
Background Tunneled catheter-related bacteremia represents one of the major complications in patients on hemodialysis,
and is associated with increased morbidity and mortality. This study aimed to evaluate the incidence of tunneled catheterrelated
bacteremia and, secondly, to identify possible factors involved in the first episode of bacteremia.
Methods This is a retrospective study of all tunneled catheters inserted between 1 January, 2005 and 31 December, 2019.
Data on patients with a tunneled catheter were analyzed for comorbidities, catheter characteristics, microbiological culture
results and variables related to the first episode of bacteremia. Patient outcomes were also assessed.
Results In the 14-year period under study, 406 tunneled catheters were implanted in 325 patients. A total of 85 cases of
tunneled catheter-related bacteremia were diagnosed, resulting in an incidence of 0.40 per 1000 catheter days (81.1%
after 6 months of implantation). The predominant microorganisms isolated were Gram-positive organisms: Staphylococcus
epidermidis (48.4%); Staphylococcus aureus (28.0%). We found no significant differences in time to catheter removal for
infections or non-infection-related reasons. The jugular vein, the Palindrome® catheter, and being the first vascular access
were protective factors for the first episode of bacteremia. The 30-day mortality rate from the first tunneled catheter-related
bacteremia was 8.7%.
Conclusions The incidence of bacteremia in our study was low and did not seem to have a relevant impact on catheter survival.
S. epidermidis was the most frequently isolated microorganism, followed by S. aureus. We identified Palindrome®
catheter, jugular vein, and being the first vascular access as significant protective factors against tunneled catheter-related
bacteremia.
application/pdf
10 p.
eng
SPRINGER
JOURNAL OF NEPHROLOGY, 36 (1), 203-212.
https://link.springer.com/article/10.1007/s40620-022-01408-8
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Bacteremia
Bloodstream
Hemodialysis
Tunneled catheter
Tunneled catheter-related bacteremia in hemodialysis patients: incidence, risk factors and outcomes. A 14-year observational study
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Medicina
JOURNAL OF NEPHROLOGY
36
1
203
212
https://ror.org/03yxnpp24
ORIGINAL
Tunneled catheter‑related...pdf
Tunneled catheter‑related...pdf
application/pdf
835333
https://idus.us.es/bitstream/11441/144251/1/Tunneled%20catheter%e2%80%91related...pdf
ffe77aec908e306b8ef7382159edc024
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1138
https://idus.us.es/bitstream/11441/144251/2/license.txt
d037f995682d8bf558422b812ec0f36c
MD5
2
open access
11441/144251
oai:idus.us.es:11441/144251
2024-02-14 14:28:21.905
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/638302024-02-12T22:12:56Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Martínez Martínez, Luis
Joyanes, Providencia
Moreno-Suárez, Ana Isabel
Perea Pérez, Evelio José
2017-08-17T09:01:15Z
2017-08-17T09:01:15Z
2001-07-31
2019
Martínez Martínez, L., Joyanes, P., Moreno-Suárez, A.I. y Perea Pérez, E.J. (2001). Activities of gemifloxacin and five other antimicrobial agents against Listeria monocytogenes and coryneform bacteria isolated from clinical samples. Antimicrobial Agents and Chemotherapy, 45 (8), 2390-2392.
0066-4804 (impreso)
1098-6596 (electrónico)
http://hdl.handle.net/11441/63830
10.1128/AAC.45.8.2390-2392.2001
The in vitro activities of gemifloxacin, ciprofloxacin, ampicillin, doxycycline, gentamicin, and vancomycin were evaluated against 15 Listeria monocytogenes strains and 205 coryneform bacteria isolated from clinical samples. The percentages of strains inhibited by gemifloxacin at 0.5 μg/ml were 100% (L. monocytogenes), 93.3% (Brevibacterium spp.), 90% (Corynebacterium minutissimum), 42.5% (Corynebacterium amycolatum), 20% (Corynebacterium striatum), 12.5% (Corynebacterium jeikeium), and 10% (Corynebacterium urealyticum). One hundred percent of the L. monocytogenes strains were inhibited by 0.25 μg of gemifloxacin per ml, whereas 0% of the strains were inhibited by 0.25 μg of ciprofloxacin per ml. Vancomycin at 2 μg/ml inhibited all strains. Doxycycline and gentamicin at 4 μg/ml inhibited 94 and 49% of the strains, respectively, while ampicillin at 0.5, 2, and 8 μg/ml inhibited 24, 61, and 66% of the strains, respectively. It is concluded that gemifloxacin shows good in vitro activity against L. monocytogenes and coryneform bacteria except C. jeikeium and C. urealyticum.
application/pdf
eng
American Society for Microbiology
Antimicrobial Agents and Chemotherapy, 45 (8), 2390-2392.
http://dx.doi.org/10.1128/AAC.45.8.2390-2392.2001
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/embargoAccess
Activities of gemifloxacin and five other antimicrobial agents against Listeria monocytogenes and coryneform bacteria isolated from clinical samples
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Antimicrobial Agents and Chemotherapy
45
8
2390
2392
https://ror.org/03yxnpp24
3 p.
ORIGINAL
Activities of gemifloxacin and five other antimicrobial agents against Listeria monocytogenes and coryneform bacteria isolated from clinical samples.pdf
Activities of gemifloxacin and five other antimicrobial agents against Listeria monocytogenes and coryneform bacteria isolated from clinical samples.pdf
application/pdf
55463
https://idus.us.es/bitstream/11441/63830/1/Activities%20of%20gemifloxacin%20and%20five%20other%20antimicrobial%20agents%20against%20Listeria%20monocytogenes%20and%20coryneform%20bacteria%20isolated%20from%20clinical%20samples.pdf
6933d06ced33476b4b0a46eb1629bd91
MD5
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open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/63830/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/63830/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/63830
oai:idus.us.es:11441/63830
2024-02-12 23:12:56.688
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1486862024-02-13T20:06:43Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Jiménez Guerrero, Irene
López Baena, Francisco Javier
Borrero de Acuña, José Manuel
Pérez Montaño, Francisco de Asís
2023-09-06T14:09:58Z
2023-09-06T14:09:58Z
2023
Jiménez Guerrero, I., López Baena, F.J., Borrero de Acuña, J.M. y Pérez Montaño, F.d.A. (2023). Membrane vesicle engineering with “à la carte” bacterial-immunogenic molecules for organism-free plant vaccination. Microbial Biotechnology. https://doi.org/10.1111/1751-7915.14323.
1751-7907
1751-7915
https://hdl.handle.net/11441/148686
10.1111/1751-7915.14323
The United Nations heralds a world population exponential increase exceeding 9.7 billion by 2050. This poses the challenge of covering the nutritional needs of an overpopulated world by the hand of preserving the environment. Extensive agriculture practices harnessed the employment of fertilizers and pesticides to boost crop productivity and prevent economic and harvest yield losses attributed to plagues and diseases. Unfortunately, the concomitant hazardous effects stemmed from such agriculture techniques are cumbersome, that is, biodiversity loss, soils and waters contaminations, and human and animal poisoning. Hence, the so-called ‘green agriculture’ research revolves around designing novel biopesticides and plant growth-promoting bio-agents to the end of curbing the detrimental effects. In this field, microbe–plant interactions studies offer multiple possibilities for reshaping the plant holobiont physiology to its benefit. Along these lines, bacterial extracellular membrane vesicles emerge as an appealing molecular tool to capitalize on. These nanoparticles convey a manifold of molecules that mediate intricate bacteria–plant interactions including plant immunomodulation. Herein, we bring into the spotlight bacterial extracellular membrane vesicle engineering to encase immunomodulatory effectors into their cargo for their application as biocontrol agents. The overarching goal is achieving plant priming by deploying its innate immune responses thereby preventing upcoming infections.
Junta de Andalucía ProyExcel_00450, EMERGIA20_00048
Ministerio de Ciencia e Innovación PID2019-107634RB-I00, PID2020-118279R, PID2021-122395OA-I00, TED2021-130357B-I00
application/pdf
13 p.
eng
Wiley-Blackwell
Microbial Biotechnology.
ProyExcel_00450
EMERGIA20_00048
PID2019-107634RB-I00
PID2020-118279R
PID2021-122395OA-I00
TED2021-130357B-I00
https://doi.org/10.1111/1751-7915.14323
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Membrane vesicle engineering with “à la carte” bacterial-immunogenic molecules for organism-free plant vaccination
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Junta de Andalucía
Ministerio de Ciencia e Innovación (MICIN). España
Microbial Biotechnology
https://ror.org/03yxnpp24
ORIGINAL
Membrane vesicle.pdf
Membrane vesicle.pdf
application/pdf
5758029
https://idus.us.es/bitstream/11441/148686/1/Membrane%20vesicle.pdf
0b029c075af89c424101cd1dbe042e79
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1142
https://idus.us.es/bitstream/11441/148686/2/license.txt
4ccdb332745e121f04fdde82917fec0c
MD5
2
open access
11441/148686
oai:idus.us.es:11441/148686
2024-02-13 21:06:43.947
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/412032024-01-24T17:42:54Zcom_11441_11044com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11045col_11441_10904
Molina, José
Cisneros, José Miguel
Fernández-Cuenca, Felipe
Rodríguez-Baño, Jesús
Ribera, Anna
Beceiro Casas, Alejandro
Martínez-Martínez, Luis
Pascual Hernández, Álvaro
Bou, Germán
Vila, Jordi
Pachón Díaz, Jerónimo
2016-05-13T14:31:39Z
2016-05-13T14:31:39Z
2010-12
0095-1137
http://hdl.handle.net/11441/41203
http://dx.doi.org/10.1128/jcm.01216-10
https://idus.us.es/xmlui/handle/11441/41203
Two hundred twenty-one isolates of Acinetobacter baumannii and 15 of Acinetobacter genospecies 3 (AG3) were consecutively collected in a 30-day period during the nationwide project GEIH-Ab2000. Nosocomial acquisition (P = 0.01), intensive care unit admission (P = 0.02), and antibiotic pressure (P = 0.03) were observed to be lower in the AG3 group. AG3 isolates were more frequently implied in wound infections (P = 0.05), while A. baumannii tended to be recovered from respiratory samples (P = 0.08). To our knowledge, this is the first report analyzing the clinical differences among Acinetobacter genospecies, with our findings suggesting that clinical features of AG3 may not be equivalent to those traditionally described
for A. baumannii
application/pdf
eng
American Society for Microbiology
Journal of Clinical Microbiology, 48(12), 4623–4626
http://dx.doi.org/10.1128/jcm.01216-10
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Clinical Features of Infections and Colonization by Acinetobacter Genospecies 3
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Medicina
Universidad de Sevilla. Departamento de Microbiología
4 p.
ORIGINAL
6680683.pdf
6680683.pdf
application/pdf
54563
https://idus.us.es/bitstream/11441/41203/1/6680683.pdf
97b3f78f5874beaed789c74b1651f25d
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/41203/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/41203/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/41203
oai:idus.us.es:11441/41203
2024-01-24 18:42:54.474
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/559242024-02-12T22:06:47Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Jiménez Guerrero, Irene
Pérez Montaño, Francisco de Asís
Medina Morillas, Carlos
Ollero Márquez, Francisco Javier
López Baena, Francisco Javier
2017-03-16T13:07:50Z
2017-03-16T13:07:50Z
2015
Jiménez Guerrero, I., Pérez Montaño, F., Medina, C., Ollero Márquez, F.J. y López Baena, F.J. (2015). NopC is a rhizobium-specific type 3 secretion system effector secreted by sinorhizobium (ensifer) fredii HH103. Plos One, 10 (11), e0142866-1-e0142866-17.
1932-6203
http://hdl.handle.net/11441/55924
10.1371/journal.pone.0142866
Sinorhizobium (Ensifer) fredii HH103 is a broad host-range nitrogen-fixing bacterium able to nodulate many legumes, including soybean. In several rhizobia, root nodulation is influenced by proteins secreted through the type 3 secretion system (T3SS). This specialized secretion apparatus is a common virulence mechanism of many plant and animal pathogenic bacteria that delivers proteins, called effectors, directly into the eukaryotic host cells where they interfere with signal transduction pathways and promote infection by suppressing host defenses. In rhizobia, secreted proteins, called nodulation outer proteins (Nops), are involved in hostrange determination and symbiotic efficiency. S. fredii HH103 secretes at least eight Nops through the T3SS. Interestingly, there are Rhizobium-specific Nops, such as NopC, which do not have homologues in pathogenic bacteria. In this work we studied the S. fredii HH103 nopC gene and confirmed that its expression was regulated in a flavonoid-, NodD1-and TtsI-dependent manner. Besides, in vivo bioluminescent studies indicated that the S. fredii HH103 T3SS was expressed in young soybean nodules and adenylate cyclase assays confirmed that NopC was delivered directly into soybean root cells by means of the T3SS machinery. Finally, nodulation assays showed that NopC exerted a positive effect on symbiosis with Glycine max cv. Williams 82 and Vigna unguiculata. All these results indicate that NopC can be considered a Rhizobium-specific effector secreted by S. fredii HH103
Junta de Andalucía P11-CVI-7050
Ministerio de Economía y Competitividad AGL2012-38831
Universidad de Sevilla
application/pdf
eng
Public Library of Science
Plos One, 10 (11), e0142866-1-e0142866-17.
P11-CVI-7050
info:eu-repo/grantAgreement/MINECO/AGL2012-38831
http://dx.doi.org/10.1371/journal.pone.0142866
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Adenylate cyclase assay
Cowpea
Gene expression regulation
Rhizobium
NopC is a rhizobium-specific type 3 secretion system effector secreted by sinorhizobium (ensifer) fredii HH103
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Plos One
10
11
e0142866-1
e0142866-17
https://ror.org/03yxnpp24
17 p.
ORIGINAL
NopC is a Rhizobium.pdf
NopC is a Rhizobium.pdf
application/pdf
2396515
https://idus.us.es/bitstream/11441/55924/1/NopC%20is%20a%20Rhizobium.pdf
06bdd10bea318db7268a62cc70d2ba57
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/55924/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
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open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/55924/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/55924
oai:idus.us.es:11441/55924
2024-02-12 23:06:47.689
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/648472024-02-14T11:12:07Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Pascual Hernández, Álvaro
García Luque, Isabel
Ballesta Mudarra, Sofía
Perea Pérez, Evelio José
2017-09-28T16:19:10Z
2017-09-28T16:19:10Z
1999-02-15
Pascual Hernández, Á., García Luque, I., Ballesta Mudarra, S. y Perea Pérez, E.J. (1999). Uptake and intracellular activity of moxifloxacin in human neutrophils and tissue-cultured epithelial cells. Antimicrobial Agents and Chemotherapy, 43 (1), 12-15.
0066-4804 (impreso)
1098-6596 (electrónico)
http://hdl.handle.net/11441/64847
The penetration by moxifloxacin of human neutrophils (polymorphonuclear leukocytes [PMN]) and tissue-cultured epithelial cells (McCoy cells) was evaluated by a fluorometric assay. At extracellular concentrations of 5 mg/liter, the cellular-to-extracellular concentration ratios (C/E) of moxifloxacin in PMN and McCoy cells were 10.9 ± 1.0 and 8.7 ± 1.0, respectively (20 min; 37°C). The uptake of moxifloxacin by PMN was rapid, reversible, nonsaturable (at extracellular concentrations ranging from 1 to 50 μg/ml), and not affected by cell viability. The uptake of moxifloxacin was affected by external pH and the environmental temperature. The incubation of PMN in the presence of sodium fluoride, sodium cyanide, and carbonyl cyanide m-chlorophenylhydrazone significantly decreased the C/E of this agent. Neither PMN stimulation nor phagocytosis of opsonized Staphylococcus aureus significantly affected the uptake of moxifloxacin by human PMN. This agent, at concentrations of 0.5, 1, and 5 mg/liter, induced a significant reduction in the survival of intracellular S. aureus in human PMN. In summary, moxifloxacin reaches much higher intracellular concentrations within phagocytic and nonphagocytic cells than extracellular ones, remaining active inside the neutrophils.
application/pdf
eng
American Society for Microbiology
Antimicrobial Agents and Chemotherapy, 43 (1), 12-15.
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Uptake and intracellular activity of moxifloxacin in human neutrophils and tissue-cultured epithelial cells
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Antimicrobial Agents and Chemotherapy
43
1
12
15
https://ror.org/03yxnpp24
4 p.
ORIGINAL
Uptake and intracellular activity of moxifloxacin in human neutrophils and tissue-cultured epithelial cells.pdf
Uptake and intracellular activity of moxifloxacin in human neutrophils and tissue-cultured epithelial cells.pdf
application/pdf
87053
https://idus.us.es/bitstream/11441/64847/1/Uptake%20and%20intracellular%20activity%20of%20moxifloxacin%20in%20human%20neutrophils%20and%20tissue-cultured%20epithelial%20cells.pdf
e6b3d8055df948413c906754f4175f21
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/64847/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/64847/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/64847
oai:idus.us.es:11441/64847
2024-02-14 12:12:07.593
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1547272024-02-06T15:01:28Zcom_11441_11044com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11045col_11441_10904
López-Cortes, Luis Eduardo
Velasco Ramírez, María del Carmen
Retamar Gentil, Pilar
Toro López, María Dolores del
Gálvez Acebal, Juan
Cueto López, Marina de
García Luque, Isabel
Pascual Hernández, Álvaro
Rodríguez-Baño, Jesús
2024-02-06T15:01:28Z
2024-02-06T15:01:28Z
2015
López-Cortes, L.E., Velasco Ramírez, M.d.C., Retamar Gentil, P., Toro López, M.D.d., Gálvez Acebal, J., Cueto López, M.d.,...,Rodríguez-Baño, J. (2015). Is reduced vancomycin susceptibility a factor associated with poor prognosis in MSSA bacteraemia?. Journal of Antimicrobial Chemotherapy, 70 (9), 2652-2660. https://doi.org/10.1093/jac/dkv133.
0305-7453
https://hdl.handle.net/11441/154727
10.1093/jac/dkv133
Objectives: The known data about the influence of vancomycin MIC on Staphylococcus aureus bacteraemia are
contradictory. Our objective was to study the possible impact of vancomycin MIC ≥1.5 mg/L on short- and
medium-term mortality.
Methods: A prospective cohort study was carried out from March 2008 to January 2011 on adult patients with
MSSA bacteraemia admitted to a tertiary hospital located in Seville (Spain). We studied the relationship between
vancomycin MIC, accessory gene regulator (agr) type and absence of d-haemolysin and poor prognosis. All isolates were genotyped by PFGE. Multivariate analysis, including a propensity score for having a vancomycin MIC of
≥1.5 mg/L, was performed by Cox regression.
Results: One-hundred and thirty-five episodes of bacteraemia due to MSSAwere included in the analysis. Twentynine (21.5%) isolates had a vancomycin MIC of ≥1.5 mg/L by Etest. There were no differences in agr distribution or
absence of d-haemolysin between isolates with reduced vancomycin susceptibility (RVS) and those without. RVS
was not more frequent in specific clones; RVS was not associated with higher 14 or 30 day crude mortality
SQ1 (RR¼0.44, 95% CI¼0.14 –1.35; and RR¼1.01, 95% CI¼0.52 –1.96) rates, and it did not show higher rates of
complicated bacteraemia (14.2% versus 13.8%, P¼0.61). Cox regression analysis did not significantly modify
the results for 14 day mortality (HR¼0.39, 95% CI¼0.11 –1.34) or 30 day mortality (HR¼0.89, 95%
CI¼0.39 –2.04).
Conclusions: Contrary to previously published data, we did not find a relationship between RVS and higher mortality in patients with MSSA bacteraemia and we did not find a link with higher complicated bacteraemia rates.
application/pdf
9 p.
eng
Oxford University Press
Journal of Antimicrobial Chemotherapy, 70 (9), 2652-2660.
https://academic.oup.com/jac/article/70/9/2652/720275
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
S. aureus
MICs
Virulence factors
Is reduced vancomycin susceptibility a factor associated with poor prognosis in MSSA bacteraemia?
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Medicina
Journal of Antimicrobial Chemotherapy
70
9
2652
2660
ORIGINAL
Is reduced...pdf
Is reduced...pdf
application/pdf
707025
https://idus.us.es/bitstream/11441/154727/1/Is%20reduced...pdf
f2d9752768194cdeed1b8a24e3b35bf4
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1142
https://idus.us.es/bitstream/11441/154727/2/license.txt
4ccdb332745e121f04fdde82917fec0c
MD5
2
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11441/154727
oai:idus.us.es:11441/154727
2024-02-06 16:01:28.427
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1562812024-03-14T16:05:11Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Borrero de Acuña, José Manuel
Poblete Castro, Ignacio
2024-03-14T16:05:11Z
2024-03-14T16:05:11Z
2023-02
Borrero de Acuña, J.M. y Poblete Castro, I. (2023). Rational engineering of natural polyhydroxyalkanoates producing microorganisms for improved synthesis and recovery. Microbial Biotechnology, 16 (2), 262-285. https://doi.org/10.1111/1751-7915.14109.
1751-7907
1751-7915
https://hdl.handle.net/11441/156281
10.1111/1751-7915.14109
Microbial production of biopolymers derived from renewable substrates and waste streams reduces our heavy reliance on petrochemical plastics. One of the most important biodegradable polymers is the family of polyhydroxyalkanoates (PHAs), naturally occurring intracellular polyoxoesters produced for decades by bacterial fermentation of sugars and fatty acids at the industrial scale. Despite the advances, PHA production still suffers from heavy costs associated with carbon substrates and downstream processing to recover the intracellular product, thus restricting market positioning. In recent years, model-aided metabolic engineering and novel synthetic biology approaches have spurred our understanding of carbon flux partitioning through competing pathways and cellular resource allocation during PHA synthesis, enabling the rational design of superior biopolymer producers and programmable cellular lytic systems. This review describes these attempts to rationally engineering the cellular operation of several microbes to elevate PHA production on specific substrates and waste products. We also delve into genome reduction, morphology, and redox cofactor engineering to boost PHA biosynthesis. Besides, we critically evaluate engineered bacterial strains in various fermentation modes in terms of PHA productivity and the period required for product recovery.
Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT) 1210332
Agencia Nacional de Investigación y Desarrollo (ANID) INACH ACT192057, INACH RG_17_19
Junta de Andalucía EMERGIA20_00048
application/pdf
24 p.
eng
Wiley-Blackwell
Microbial Biotechnology, 16 (2), 262-285.
1210332
INACH ACT192057
INACH RG_17_19
EMERGIA20_00048
https://doi.org/10.1111/1751-7915.14109
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Rational engineering of natural polyhydroxyalkanoates producing microorganisms for improved synthesis and recovery
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT). Chile
Agencia Nacional de Investigación y Desarrollo (ANID). Chile
Junta de Andalucía
Microbial Biotechnology
16
2
262
285
ORIGINAL
Rational engineering.pdf
Rational engineering.pdf
Versión publicada
application/pdf
6608456
https://idus.us.es/bitstream/11441/156281/1/Rational%20engineering.pdf
a4b970e339288866091920dd51a09284
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1142
https://idus.us.es/bitstream/11441/156281/2/license.txt
4ccdb332745e121f04fdde82917fec0c
MD5
2
open access
11441/156281
oai:idus.us.es:11441/156281
2024-03-14 17:05:11.387
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1395682024-02-14T20:32:06Zcom_11441_11044com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11045col_11441_10904
Rodríguez-Baño, Jesús
Gutiérrez Gutiérrez, Belén
Pascual Hernández, Álvaro
2022-11-17T16:17:17Z
2022-11-17T16:17:17Z
2021-02-24
Rodríguez-Baño, J., Gutiérrez Gutiérrez, B. y Pascual Hernández, Á. (2021). CON: carbapenems are NOT necessary for all infections caused by ceftriaxone-resistant enterobacterales. JAC-antimicrobial resistance, 3 (1), dlaa112. https://doi.org/10.1093/jacamr/dlaa112.
2632-1823
https://hdl.handle.net/11441/139568
10.1093/jacamr/dlaa112
Carbapenems are considered the drugs of choice for the treatment of serious infections caused by ceftriaxone resistant Enterobacterales. However, because of the dramatic increase in carbapenem-resistant organisms
worldwide, finding alternatives to carbapenems is a must. The potential options include b-lactam/b-lactamase
inhibitor combinations, temocillin, cephamycins and some non-b-lactam drugs. The most controversial is pipera cillin/tazobactam; the results of the MERINO trial are challenged because the isolates of patients with worse out comes were frequently not susceptible to piperacillin/tazobactam when studied by reference methods, and also
because the drug was not administered in extended infusion. Other potential options are briefly discussed. We
conclude that carbapenems are not necessary for all patients with infections caused by ceftriaxone-resistant
Enterobacterales.
application/pdf
4 p.
eng
JAC-antimicrobial resistance, 3 (1), dlaa112.
RD16/0016/0001
https://academic.oup.com/jacamr/article/3/1/dlaa112/6146932
Atribución-NoComercial 4.0 Internacional
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
Ceftriaxone
Carbapenem
Cephamycins
Lactams
Infections
CON: carbapenems are NOT necessary for all infections caused by ceftriaxone-resistant enterobacterales
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Medicina
Universidad de Sevilla. Departamento de Microbiología
Subdirección General de Redes y Centros de Investigación Cooperativa, Ministerio de Economía, Industria y Competitividad
JAC-antimicrobial resistance
3
1
dlaa112
https://ror.org/03yxnpp24
ORIGINAL
CON Carbapenems...pdf
CON Carbapenems...pdf
application/pdf
256206
https://idus.us.es/bitstream/11441/139568/1/CON%20Carbapenems...pdf
32983d58e89aedf6b0c01f4382183be1
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/139568/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/139568
oai:idus.us.es:11441/139568
2024-02-14 21:32:06.023
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/972782024-02-12T22:00:39Zcom_11441_10903com_11441_10802com_11441_10690com_11441_10923col_11441_10904col_11441_10924
Vinardell González, José María
Ollero Márquez, Francisco Javier
Hidalgo Perea, Ángeles
López Baena, Francisco Javier
Medina Morillas, Carlos
Vangelov, Kalojan Ivanov
Parada, Maribel
Madinabeitia Peiró, Nuria
Espuny Gómez, María del Rosario
Bellogín Izquierdo, Ramón Andrés
Soria Díaz, María Eugenia
Gil Serrano, Antonio Miguel
Ruiz Sainz, José Enrique
2020-06-01T07:56:01Z
2020-06-01T07:56:01Z
2004
Vinardell González, J.M., Ollero Márquez, F.J., Hidalgo Perea, Á., López Baena, F.J., Medina Morillas, C., Vangelov, K.I.,...,Ruiz Sainz, J.E. (2004). NolR Regulates Diverse Symbiotic Signals of Sinorhizobium fredii HH103. Molecular Plant-Microbe Interactions, 17 (6), 676-685.
0894-0282
1943-7706
https://hdl.handle.net/11441/97278
10.1094/MPMI.2004.17.6.676
We have investigated in Sinorhizobium fredii HH103-1
(=HH103 Strr
) the influence of the nolR gene on the production of three different bacterial symbiotic signals: Nod
factors, signal responsive (SR) proteins, and exopolysaccharide (EPS). The presence of multiple copies of nolR (in
plasmid pMUS675) repressed the transcription of all the
flavonoid-inducible genes analyzed: nodA, nodD1, nolO,
nolX, noeL, rhcJ, hesB, and y4pF. Inactivation of nolR
(mutant SVQ517) or its overexpression (presence of
pMUS675) altered the amount of Nod factors detected.
Mutant SVQ517 produced Nod factors carrying N-methyl
residues at the nonreducing N-acetyl-glucosamine, which
never have been detected in S. fredii HH103. Plasmid
pMUS675 increased the amounts of EPS produced by
HH103-1 and SVQ517. The flavonoid genistein repressed
EPS production of HH103-1 and SVQ517 but the presence
of pMUS675 reduced this repression. The presence of plasmid pMUS675 clearly decreased the secretion of SR proteins. Inactivation, or overexpression, of nolR decreased the
capacity of HH103 to nodulate Glycine max. However,
HH103-1 and SVQ517 carrying plasmid pMUS675 showed
enhanced nodulation capacity with Vigna unguiculata. The
nolR gene was positively identified in all S. fredii strains
investigated, S. xinjiangense CCBAU110, and S. saheli
USDA4102. Apparently, S. teranga USDA4101 does not contain this gene.
Comisión Interministerial de Ciencia y Tecnología (CICYT), Gobierno de España BIO99-0614-C03 y BOS2002-04164-C03-02
application/pdf
10 p.
eng
The American Phytopathological Society
Molecular Plant-Microbe Interactions, 17 (6), 676-685.
BIO99-0614-C03
BOS2002-04164-C03-02
https://doi.org/10.1094/MPMI.2004.17.6.676
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
LCO
Symbiotic interaction
NolR Regulates Diverse Symbiotic Signals of Sinorhizobium fredii HH103
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Química orgánica
Comisión Interministerial de Ciencia y Tecnología (CICYT). España
Molecular Plant-Microbe Interactions
17
6
676
685
https://ror.org/03yxnpp24
ORIGINAL
MPMI.2004.17.6.676.pdf
MPMI.2004.17.6.676.pdf
application/pdf
198529
https://idus.us.es/bitstream/11441/97278/1/MPMI.2004.17.6.676.pdf
bdfa3fe744d55c2ca1d203a6b3a401e7
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/97278/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/97278
oai:idus.us.es:11441/97278
2024-02-12 23:00:39.064
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1401762024-02-14T09:16:44Zcom_11441_10999com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11000col_11441_10904
Escrihuela-Vidal, Francesc
López Cortés, Luis Eduardo
Escolà Vergé, Laura
Alarcón González, Arístides de
Cuervo, Guillermo
Sánchez-Porto, Antonio
Araji Tiliani, Omar
Cueto López, Marina de
Lepe Jiménez, José Antonio
2022-12-05T15:23:46Z
2022-12-05T15:23:46Z
2021-06
Escrihuela-Vidal, F., López Cortés, L.E., Escolà Vergé, L., Alarcón González, A.d., Cuervo, G., Sánchez-Porto, A.,...,Lepe Jiménez, J.A. (2021). Clinical Features and Outcomes of Streptococcus anginosus Group Infective Endocarditis: A Multicenter Matched Cohort Study. Open Forum Infectious Diseases, 8 (6), ofab163. https://doi.org/10.1093/ofid/ofab163.
2328-8957
https://hdl.handle.net/11441/140176
10.1093/ofid/ofab163
Background
Although Streptococcus anginosus group (SAG) endocarditis is considered a severe disease associated with abscess formation and embolic events, there is limited evidence to support this assumption.
Methods
We performed a retrospective analysis of prospectively collected data from consecutive patients with definite SAG endocarditis in 28 centers in Spain and Italy. A comparison between cases due to SAG endocarditis and viridans group streptococci (VGS) or Streptococcus gallolyticus group (SGG) was performed in a 1:2 matched analysis.
Results
Of 5336 consecutive cases of definite endocarditis, 72 (1.4%) were due to SAG and matched with 144 cases due to VGS/SGG. SAG endocarditis was community acquired in 64 (88.9%) cases and affected aortic native valve in 29 (40.3%). When comparing SAG and VGS/SGG endocarditis, no significant differences were found in septic shock (8.3% vs 3.5%, P = .116); valve disorder, including perforation (22.2% vs 18.1%, P = .584), pseudoaneurysm (16.7% vs 8.3%, P = .108), or prosthesis dehiscence (1.4% vs 6.3%, P = .170); paravalvular complications, including abscess (25% vs 18.8%, P = .264) and intracardiac fistula (5.6% vs 3.5%, P = .485); heart failure (34.7% vs 38.9%, P = .655); or embolic events (41.7% vs 32.6%, P = .248). Indications for surgery (70.8% vs 70.8%; P = 1) and mortality (13.9% vs 16.7%; P = .741) were similar between groups.
Conclusions
SAG endocarditis is an infrequent but serious condition that presents a prognosis similar to that of VGS/SGG.
application/pdf
7 p.
eng
Oxford University Press
Open Forum Infectious Diseases, 8 (6), ofab163.
REIPI RD16/0016/0005
https://academic.oup.com/ofid/article/8/6/ofab163/6199901
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
infective endocarditis
Streptococcus anginosus
viridans group streptococci
Streptococcus gallolyticus
Clinical Features and Outcomes of Streptococcus anginosus Group Infective Endocarditis: A Multicenter Matched Cohort Study
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Cirugía
Instituto de Salud Carlos III
Open Forum Infectious Diseases
8
6
ofab163
https://ror.org/03yxnpp24
ORIGINAL
Clinical Features and Outcomes of Streptococcus anginosus Group Infective Endocarditis. A Multicenter Matched Cohort Study.pdf
Clinical Features and Outcomes of Streptococcus anginosus Group Infective Endocarditis. A Multicenter Matched Cohort Study.pdf
application/pdf
206024
https://idus.us.es/bitstream/11441/140176/1/Clinical%20Features%20and%20Outcomes%20of%20Streptococcus%20anginosus%20Group%20Infective%20Endocarditis.%20A%20Multicenter%20Matched%20Cohort%20Study.pdf
3d655fb54f21182c3601ba4655d6d779
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/140176/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/140176
oai:idus.us.es:11441/140176
2024-02-14 10:16:44.022
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1562752024-03-14T15:07:52Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Mingers, Toni
Barthels, Stefan
Mass, Violetta
Borrero de Acuña, José Manuel
Biedendieck, Rebekka
Cooke, Ana
Dailey, Tamara A.
Gerdes, Svetlana
Blankenfeldt, Wulf
Dailey, Harry A.
Warren, Martin J.
Jahn, Martina
Jahn, Dieter
2024-03-14T15:07:52Z
2024-03-14T15:07:52Z
2024-03-13
Mingers, T., Barthels, S., Mass, V., Borrero de Acuña, J.M., Biedendieck, R., Cooke, A.,...,Jahn, D. (2024). The alternative coproporphyrinogen III oxidase (CgoN) catalyzes the oxygen-independent conversion of coproporphyrinogen III into coproporphyrin III. Frontiers in Microbiology, 15, 1378989. https://doi.org/10.3389/fmicb.2024.1378989.
1664-302X
https://hdl.handle.net/11441/156275
10.3389/fmicb.2024.1378989
Nature utilizes three distinct pathways to synthesize the essential enzyme cofactor heme. The coproporphyrin III-dependent pathway, predominantly present in Bacillaceae, employs an oxygen-dependent coproporphyrinogen III oxidase (CgoX) that converts coproporphyrinogen III into coproporphyrin III. In this study, we report the bioinformatic-based identification of a gene called ytpQ, encoding a putative oxygen-independent counterpart, which we propose to term CgoN, from Priestia (Bacillus) megaterium. The recombinantly produced, purified, and monomeric YtpQ (CgoN) protein is shown to catalyze the oxygen-independent conversion of coproporphyrinogen III into coproporphyrin III. Minimal non-enzymatic conversion of coproporphyrinogen III was observed under the anaerobic test conditions employed in this study. FAD was identified as a cofactor, and menadione served as an artificial acceptor for the six abstracted electrons, with a KM value of 3.95 μmol/L and a kcat of 0.63 per min for the substrate. The resulting coproporphyrin III, in turn, acts as an effective substrate for the subsequent enzyme of the pathway, the coproporphyrin III ferrochelatase (CpfC). Under aerobic conditions, oxygen directly serves as an electron acceptor, but is replaced by the more efficient action of menadione. An AlphaFold2 model of the enzyme suggests that YtpQ adopts a compact triangular shape consisting of three domains. The N-terminal domain appears to be flexible with respect to the rest of the structure, potentially creating a ligand binding site that opens and closes during the catalytic cycle. A catalytic mechanism similar to the oxygen-independent protoporphyrinogen IX oxidase PgoH1 (HemG), based on the flavin-dependent abstraction of six electrons from coproporphyrinogen III and their potential quinone-dependent transfer to a membrane-localized electron transport chain, is proposed.
application/pdf
13 p.
eng
Frontiers Media S.A.
Frontiers in Microbiology, 15, 1378989.
https://doi.org/10.3389/fmicb.2024.1378989
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
The alternative coproporphyrinogen III oxidase (CgoN) catalyzes the oxygen-independent conversion of coproporphyrinogen III into coproporphyrin III
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Frontiers in Microbiology
15
1378989
ORIGINAL
The alternative coproporphyrinogen.pdf
The alternative coproporphyrinogen.pdf
Versión publicada
application/pdf
3028421
https://idus.us.es/bitstream/11441/156275/1/The%20alternative%20coproporphyrinogen.pdf
4a5cde401ba285d0a10c0af37ef2cc70
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1142
https://idus.us.es/bitstream/11441/156275/2/license.txt
4ccdb332745e121f04fdde82917fec0c
MD5
2
open access
11441/156275
oai:idus.us.es:11441/156275
2024-03-14 16:07:52.809
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/233472024-02-14T08:47:33Zcom_11441_10903com_11441_10802com_11441_10690com_11441_11084com_11441_10983com_11441_10923col_11441_10904col_11441_11085col_11441_10924
Gil Serrano, Antonio Miguel
Rodríguez Carvajal, Miguel Ángel
Tejero Mateo, María Pilar
Espartero Sánchez, José Luis
Menéndez Gallego, Manuel
Corzo, Javier
Ruiz Sainz, José Enrique
Buendía Clavería, Ana María
2015-03-03T13:35:24Z
2015-03-03T13:35:24Z
1999
1932-6203
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1220493/pdf/10477263.pdf
http://hdl.handle.net/11441/23347
https://idus.us.es/xmlui/handle/11441/23347
eng
PLoS ONE, 342, 527-535
Atribución-NoComercial-SinDerivadas 4.0 España
http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
Structural determination of a 5-acetamido-3,5,7,9-tetradeoxy- 7(3-hydroxybutyramido)-L-glycero-L-manno-nonulosonic acid- containing homopolysaccharide isolated from Sinorhizobium fredii HH103
info:eu-repo/semantics/article
Universidad de Sevilla. Departamento de Química Orgánica
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica
https://ror.org/03yxnpp24
ORIGINAL
file_1.pdf
application/pdf
211467
https://idus.us.es/bitstream/11441/23347/1/file_1.pdf
d8f06d12376515b33f279b01d84ff6f8
MD5
1
open access
11441/23347
oai:idus.us.es:11441/23347
2024-02-14 09:47:33.514
open access
idUS - Universidad de Sevilla
idus@us.es
oai:idus.us.es:11441/664932024-02-17T16:51:56Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
López-Rojas, Rafael
Domínguez-Herrera, J.
Mcconnell, Michael
Docobo Pérez, Fernando
Smani, Younes
Pachón Díaz, Jerónimo
2017-11-22T18:23:29Z
2017-11-22T18:23:29Z
2011-01-07
López Rojas, R., Domínguez-Herrera, J., Mcconnell, M., Dacobo Pérez, F., Smani, Y. y Pachón Díaz, J. (2011). Impaired Virulence and In Vivo Fitness of Colistin-Resistant Acinetobacter baumannii. The Journal of Infectious Diseases, 203 (4), 545-548.
0022-1899 (impreso)
1537-6613 (electrónico)
http://hdl.handle.net/11441/66493
10.1093/infdis/jiq086
20126064
Acinetobacter baumannii (American Type Culture Collection strain 19606) acquires mutations in the pmrB gene during the in vitro development of resistance to colistin. The colistin-resistant strain has lower affinity for colistin, reduced in vivo fitness (competition index, .016), and decreased virulence, both in terms of mortality (0% lethal dose, 6.9 vs 4.9 log colony-forming units) and survival in a mouse model of peritoneal sepsis. These results may explain the low incidence and dissemination of colistin resistance in A. baumannii in clinical settings.
REIPI RD06/0008
Ministerio de Ciencia e Innovación y Comunidad de Madrid COMBACT S-BIO-0260/2006
application/pdf
eng
Oxford University Press
The Journal of Infectious Diseases, 203 (4), 545-548.
RD06/0008
COMBACT S-BIO-0260/2006
http://dx.doi.org/10.1093/infdis/jiq086
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Acinetobacter infections
Acinetobacter baumannii
Animals
Anti-bacterial agents
Bacterial proteins
Colistin
Peritonitis
Sepsis
Impaired Virulence and In Vivo Fitness of Colistin-Resistant Acinetobacter baumannii
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. Departamento de Medicina
Ministerio de Ciencia e Innovación (MICIN). España
The Journal of Infectious Diseases
203
4
545
548
https://ror.org/03yxnpp24
4 p.
ORIGINAL
J Infect Dis.-2011-545-8.pdf
J Infect Dis.-2011-545-8.pdf
application/pdf
188663
https://idus.us.es/bitstream/11441/66493/1/J%20Infect%20Dis.-2011-545-8.pdf
74735b21722c1e5a578354fef6154b80
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/66493/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/66493/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/66493
oai:idus.us.es:11441/66493
2024-02-17 17:51:56.835
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/390802024-02-14T14:02:21Zcom_11441_11044com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11045col_11441_10904
Retamar Gentil, Pilar
López Cerero, Lorena
Muniain Ezcurra, Miguel Angel
Pascual Hernández, Álvaro
Rodríguez-Baño, Jesús
2016-03-29T17:09:13Z
2016-03-29T17:09:13Z
2013-07
0066-4804
http://hdl.handle.net/11441/39080
http://dx.doi.org/10.1128/AAC.00135-13
https://idus.us.es/xmlui/handle/11441/39080
We investigated the impact of the piperacillin-tazobactam MIC in the outcome of 39 bloodstream infections due to extended-
spectrum-B-lactamase-producing Escherichia coli. All 11 patients with urinary tract infections survived, irrespective of the MIC.
For other sources, 30-day mortality was lower for isolates with a MIC of
<2 mg/liter than for isolates with a higher MIC (0% ver-
sus 41.1%; P = 0.02)
application/pdf
eng
American Society for Microbiology
Antimicrobial Agents and Chemotherapy 57(7), 3402-3404
http://aac.asm.org/content/57/7/3402.abstract
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Antibacterianos
Estudios de cohortes
Quimioterapia combinada
Infecciones por escherichia coli
Pruebas de sensibilidad microbiana
Ácido senicilánico
Infecciones urinarias
Impact of the MIC of Piperacillin-Tazobactam on the Outcome of Patients with Bacteremia Due to Extended-Spectrum-B-Lactamase- Producing Escherichia coli
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Medicina
Universidad de Sevilla. Departamento de Microbiología
https://ror.org/03yxnpp24
ORIGINAL
20530603.pdf
20530603.pdf
application/pdf
329045
https://idus.us.es/bitstream/11441/39080/1/20530603.pdf
8f7c6e4af9eb3d6fb634a0b04b3554ef
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/39080/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/39080/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/39080
oai:idus.us.es:11441/39080
2024-02-14 15:02:21.042
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/707902024-02-13T09:10:43Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Romero Portillo, Francisco
Gil-Bernabé, Ana M.
Sáez, Carmen
Pintor Toro, José Antonio
Tortolero García, María Dolores
2018-03-05T16:10:10Z
2018-03-05T16:10:10Z
2004
Romero Portillo, F., Gil-Bernabé, A.M., Sáez, C., Pintor Toro, J.A. y Tortolero García, M.D. (2004). Securin Is a Target of the UV Response Pathway in Mammalian Cells. Molecular and Cellular Biology, 24 (7), 2720-2733.
0270-7306
https://hdl.handle.net/11441/70790
10.1128/MCB.24.7.2720-2733.2004
All eukaryotic cells possess elaborate mechanisms to protect genome integrity and ensure survival after DNA damage, ceasing proliferation and granting time for DNA repair. Securin is an inhibitory protein that is bound to a protease called Separase to inhibit sister chromatid separation until the onset of anaphase. At the metaphase-to-anaphase transition, Securin is degraded by the anaphase-promoting complex or cyclosome, and Separase contributes to the release of cohesins from the chromosome, allowing for the segregation of sister chromatids to opposite spindle poles. Here we provide evidence that human Securin (hSecurin) has a novel role in cell cycle arrest after exposure to UV light or ionizing radiation. In fact, irradiation downregulated the level of hSecurin protein, accelerating its degradation via the proteasome and reducing hSecurin mRNA translation, but the presence of hSecurin is necessary for cell proliferation arrest following UV treatment. Moreover, an alteration of UV-induced hSecurin downregulation could lead directly to the accumulation of DNA damage and the subsequent development of malignant tumors.
Ministerio de Ciencia y Tecnología SAF2002-04177-C04
Junta de Andalucía DGUI
application/pdf
eng
Molecular and Cellular Biology, 24 (7), 2720-2733.
http://dx.doi.org/10.1128/MCB.24.7.2720-2733.2004
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Cell DNA
Messenger RNA
Unclassified drug
Securin Is a Target of the UV Response Pathway in Mammalian Cells
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Anatomía Patológica
Ministerio de Ciencia y Tecnología (MCYT). España
Junta de Andalucía
Molecular and Cellular Biology
24
7
2720
2733
https://ror.org/03yxnpp24
14
ORIGINAL
securin is a target.pdf
securin is a target.pdf
application/pdf
666610
https://idus.us.es/bitstream/11441/70790/1/securin%20is%20a%20target.pdf
9e063bf71a8d032dec53d153ab775879
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
804
https://idus.us.es/bitstream/11441/70790/2/license_rdf
c1efe8e24d7281448e873be30ea326ff
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/70790/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/70790
oai:idus.us.es:11441/70790
2024-02-13 10:10:43.52
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1528822024-02-15T07:18:26Zcom_11441_10903com_11441_10802com_11441_10690com_11441_10923col_11441_10904col_11441_10924
Navarro Gómez, Pilar
Fuentes Romero, Francisco
Pérez Montaño, Francisco de Asís
Jiménez Guerrero, Irene
Alías Villegas, Cynthia
Ayala García, Paula
Almozara, Andrés
Medina Morillas, Carlos
Ollero Márquez, Francisco Javier
Rodríguez Carvajal, Miguel Ángel
Ruiz Sainz, José Enrique
López Baena, Francisco Javier
Vinardell González, José María
Acosta Jurado, Sebastián
2024-01-02T13:59:42Z
2024-01-02T13:59:42Z
2023
Navarro Gómez, P., Fuentes Romero, F., Pérez Montaño, F.d.A., Jiménez Guerrero, I., Alías Villegas, C., Ayala García, P.,...,Acosta Jurado, S. (2023). A Complex Regulatory Network Governs the Expression of Symbiotic Genes in Sinorhizobium Fredii HH103. Frontiers in Plant Science, 14, 1322435. https://doi.org/10.3389/fpls.2023.1322435.
1664-462X
https://hdl.handle.net/11441/152882
10.3389/fpls.2023.1322435
Introduction: The establishment of the rhizobium-legume nitrogen-fixing
symbiosis relies on the interchange of molecular signals between the two
symbionts. We have previously studied by RNA-seq the effect of the
symbiotic regulators NodD1, SyrM, and TtsI on the expression of the
symbiotic genes (the nod regulon) of Sinorhizobium fredii HH103 upon
treatment with the isoflavone genistein. In this work we have further
investigated this regulatory network by incorporating new RNA-seq data of
HH103 mutants in two other regulatory genes, nodD2 and nolR. Both genes
code for global regulators with a predominant repressor effect on the nod
regulon, although NodD2 acts as an activator of a small number of HH103
symbiotic genes.
Methods: By combining RNA-seq data, qPCR experiments, and
b-galactosidase assays of HH103 mutants harbouring a lacZ gene inserted
into a regulatory gene, we have analysed the regulatory relations between
the nodD1, nodD2, nolR, syrM, and ttsI genes, confirming previous data and
discovering previously unknown relations.
Results and discussion: Previously we showed that HH103 mutants in the
nodD2, nolR, syrM, or ttsI genes gain effective nodulation with Lotus
japonicus, a model legume, although with different symbiotic
performances. Here we show that the combinations of mutations in these genes led, in most cases, to a decrease in symbiotic effectiveness, although
all of them retained the ability to induce the formation of nitrogen-fixing
nodules. In fact, the nodD2, nolR, and syrM single and double mutants share
a set of Nod factors, either overproduced by them or not generated by the
wild-type strain, that might be responsible for gaining effective nodulation
with L. japonicus.
Ministerio de Ciencia e Innovación PID2019-107634RB-I00
application/pdf
19 p.
eng
Frontiers Media
Frontiers in Plant Science, 14, 1322435.
PID2019-107634RB-I00
https://doi.org/10.3389/fpls.2023.1322435
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Rhizobium-legume symbiosis
Nod factors
NodD1
NodD2
NolR
SyrM
TtsI
Symbiotic genes regulatory network
A Complex Regulatory Network Governs the Expression of Symbiotic Genes in Sinorhizobium Fredii HH103
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Química orgánica
Ministerio de Ciencia e Innovación (MICIN). España
Frontiers in Plant Science
14
1322435
https://ror.org/03yxnpp24
ORIGINAL
A complex regulatory.pdf
A complex regulatory.pdf
application/pdf
2250919
https://idus.us.es/bitstream/11441/152882/1/A%20complex%20regulatory.pdf
a6c30397b9fb887495c3267ded61624b
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1142
https://idus.us.es/bitstream/11441/152882/2/license.txt
4ccdb332745e121f04fdde82917fec0c
MD5
2
open access
11441/152882
oai:idus.us.es:11441/152882
2024-02-15 08:18:26.067
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1374932024-02-15T07:26:51Zcom_11441_11044com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11045col_11441_10904
Pierrotti, L. C
Pérez-Nadales, E.
Fernández-Ruiz, M.
Gutiérrez Gutiérrez, Belén
Tan, B. H.
Carratalà, J.
Cordero Matia, María Elisa
Pascual Hernández, Álvaro
Rodríguez-Baño, Jesús
Aguado, J. M.
2022-09-29T18:00:09Z
2022-09-29T18:00:09Z
2021
Pierrotti, L.C., Pérez-Nadales, E., Fernández-Ruiz, M., Gutiérrez Gutiérrez, B., Tan, B.H., Carratalà, J.,...,Aguado, J.M. (2021). Efficacy of β-lactam/β-lactamase inhibitors to treat extended-spectrum beta-lactamase-producing Enterobacterales bacteremia secondary to urinary tract infection in kidney transplant recipients. Transplant Infectious Disease, 23 (3), 1-16.
1398-2273
1399-3062
https://hdl.handle.net/11441/137493
10.1111/tid.13520
Background
Whether active therapy with β-lactam/β-lactamase inhibitors (BLBLI) is as affective as carbapenems for extended-spectrum β-lactamase-producing Enterobacterales (ESBL-E) bloodstream infection (BSI) secondary to urinary tract infection (UTI) in kidney transplant recipients (KTRs) remains unclear.
Methods
We retrospectively evaluated 306 KTR admitted to 30 centers from January 2014 to October 2016. Therapeutic failure (lack of cure or clinical improvement and/or death from any cause) at days 7 and 30 from ESBL-E BSI onset was the primary and secondary study outcomes, respectively.
Results
Therapeutic failure at days 7 and 30 occurred in 8.2% (25/306) and 13.4% (41/306) of patients. Hospital-acquired BSI (adjusted OR [aOR]: 4.10; 95% confidence interval [CI]: 1.50-11.20) and Pitt score (aOR: 1.47; 95% CI: 1.21-1.77) were independently associated with therapeutic failure at day 7. Age-adjusted Charlson Index (aOR: 1.25; 95% CI: 1.05-1.48), Pitt score (aOR: 1.72; 95% CI: 1.35-2.17), and lymphocyte count ≤500 cells/μL at presentation (aOR: 3.16; 95% CI: 1.42-7.06) predicted therapeutic failure at day 30. Carbapenem monotherapy (68.6%, primarily meropenem) was the most frequent active therapy, followed by BLBLI monotherapy (10.8%, mostly piperacillin-tazobactam). Propensity score (PS)-adjusted models revealed no significant impact of the choice of active therapy (carbapenem-containing vs any other regimen, BLBLI- vs carbapenem-based monotherapy) within the first 72 hours on any of the study outcomes.
Conclusions
Our data suggest that active therapy based on BLBLI may be as effective as carbapenem-containing regimens for ESBL-E BSI secondary to UTI in the specific population of KTR. Potential residual confounding and unpowered sample size cannot be excluded
16 p.
eng
Willey-Blackwell
Transplant Infectious Disease, 23 (3), 1-16.
http://doi.org/10.1111/tid.13520
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Bloodstream infection
Carbapenem-sparing regimen
Extended-spectrum β-lactamase-producing Enterobacterales
Outcomes
Urinary tract infection
Kidney transplantation
Efficacy of β-lactam/β-lactamase inhibitors to treat extended-spectrum beta-lactamase-producing Enterobacterales bacteremia secondary to urinary tract infection in kidney transplant recipients
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Medicina
Universidad de Sevilla. Departamento de Microbiología
Transplant Infectious Disease
23
3
1
16
https://ror.org/03yxnpp24
ORIGINAL
316.pdf
316.pdf
application/pdf
866412
https://idus.us.es/bitstream/11441/137493/1/316.pdf
61dcb4f705f6ed5090d5f9995ee968cd
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/137493/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/137493
oai:idus.us.es:11441/137493
2024-02-15 08:26:51.716
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1420842024-02-15T07:18:38Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Bernal Guzmán, Patricia
Civantos Jiménez, Cristina
Pacheco Sánchez, Daniel
Quesada, José M.
Filloux, Alain
Llamas, María A.
2023-01-30T07:58:58Z
2023-01-30T07:58:58Z
2023
Bernal Guzmán, P., Civantos Jiménez, C., Pacheco Sánchez, D., Quesada, J.M., Filloux, A. y Llamas, M.A. (2023). Transcriptional organization and regulation of the Pseudomonas putida K1 type VI secretion system gene cluster. Microbiology, 169 (1), 001295. https://doi.org/10.1099/mic.0.001295.
1465-2080
https://hdl.handle.net/11441/142084
10.1099/mic.0.001295
The type VI secretion system (T6SS) is an antimicrobial molecular weapon that is widespread in Proteobacteria and offers competitive advantages to T6SS-positive micro-organisms. Three T6SSs have recently been described in Pseudomonas putida KT2440 and it has been shown that one, K1-T6SS, is used to outcompete a wide range of phytopathogens, protecting plants from pathogen infections. Given the relevance of this system as a powerful and innovative mechanism of biological control, it is critical to understand the processes that govern its expression. Here, we experimentally defined two transcriptional units in the K1-T6SS cluster. One encodes the structural components of the system and is transcribed from two adjacent promoters. The other encodes two hypothetical proteins, the tip of the system and the associated adapters, and effectors and cognate immunity proteins, and it is also transcribed from two adjacent promoters. The four identified promoters contain the typical features of σ70-dependent promoters. We have studied the expression of the system under different conditions and in a number of mutants lacking global regulators. P. putida K1-T6SS expression is induced in the stationary phase, but its transcription does not depend on the stationary σ factor RpoS. In fact, the expression of the system is indirectly repressed by RpoS. Furthermore, it is also repressed by RpoN and the transcriptional regulator FleQ, an enhancer-binding protein typically acting in conjunction with RpoN. Importantly, expression of the K1-T6SS gene cluster is positively regulated by the GacS–GacA two-component regulatory system (TCS) and repressed by the RetS sensor kinase, which inhibits this TCS. Our findings identified a complex regulatory network that governs T6SS expression in general and P. putida K1-T6SS in particular, with implications for controlling and manipulating a bacterial agent that is highly relevant in biological control.
application/pdf
16 p.
eng
Microbiology Society
Microbiology, 169 (1), 001295.
BIO2017-83763-P
PID2020- 115682GB-I00
BB/N002539/1
https://dx.doi.org/10.1099/mic.0.001295
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
type VI secretion system
gene regulation
RetS
GacS–GacA
RpoN
RpoS
Pseudomonas
FleQ
Transcriptional organization and regulation of the Pseudomonas putida K1 type VI secretion system gene cluster
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Ministerio de Ciencia e Innovación (MICIN). España
Biotechnology and Biological Sciences Research Council (UK)
Microbiology
169
1
001295
https://ror.org/03yxnpp24
ORIGINAL
mic001295.pdf
mic001295.pdf
application/pdf
3496276
https://idus.us.es/bitstream/11441/142084/1/mic001295.pdf
2503b40fcdd18d8c59cb97f1ebf4265a
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/142084/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/142084
oai:idus.us.es:11441/142084
2024-02-15 08:18:38.414
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1396092024-02-14T11:15:06Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Jiménez Guerrero, Irene
Medina Morillas, Carlos
Vinardell González, José María
Ollero Márquez, Francisco Javier
López Baena, Francisco Javier
2022-11-18T17:45:41Z
2022-11-18T17:45:41Z
2022
Jiménez Guerrero, I., Medina Morillas, C., Vinardell González, J.M., Ollero Márquez, F.J. y López Baena, F.J. (2022). The Rhizobial Type 3 Secretion System: The Dr. Jekyll and Mr. Hyde in the Rhizobium–Legume Symbiosis. International Journal of Molecular Sciences, 23 (19), 11089. https://doi.org/10.3390/ijms231911089.
1661-6596
1422-0067
https://hdl.handle.net/11441/139609
10.3390/ijms231911089
Rhizobia are soil bacteria that can establish a symbiotic association with legumes. As a result, plant nodules are formed on the roots of the host plants where rhizobia differentiate to bacteroids capable of fixing atmospheric nitrogen into ammonia. This ammonia is transferred to the plant in exchange of a carbon source and an appropriate environment for bacterial survival. This process is subjected to a tight regulation with several checkpoints to allow the progression of the infection or its restriction. The type 3 secretion system (T3SS) is a secretory system that injects proteins, called effectors (T3E), directly into the cytoplasm of the host cell, altering host pathways or suppressing host defense responses. This secretion system is not present in all rhizobia but its role in symbiosis is crucial for some symbiotic associations, showing two possible faces as Dr. Jekyll and Mr. Hyde: it can be completely necessary for the formation of nodules, or it can block nodulation in different legume species/cultivars. In this review, we compile all the information currently available about the effects of different rhizobial effectors on plant symbiotic phenotypes. These phenotypes are diverse and highlight the importance of the T3SS in certain rhizobium–legume symbioses.
Ministerio de Ciencia e Innovación PID2019-107634RB-I00
Junta de Andalucía P20_00185
Universidad de Sevilla FEDER-US 1259948, FEDER-US 1250546
application/pdf
26 p.
eng
Multidisciplinary Digital Publishing Institute (MDPI)
International Journal of Molecular Sciences, 23 (19), 11089.
PID2019-107634RB-I00
P20_00185
FEDER-US 1259948
FEDER-US 1250546
https://doi.org/10.3390/ijms231911089
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Effector
Rhizobium
Symbiosis
T3SS
Type 3 secretion system
The Rhizobial Type 3 Secretion System: The Dr. Jekyll and Mr. Hyde in the Rhizobium–Legume Symbiosis
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Ministerio de Ciencia e Innovación (MICIN). España
Junta de Andalucía
Universidad de Sevilla
International Journal of Molecular Sciences
23
19
11089
https://ror.org/03yxnpp24
ORIGINAL
The Rhizobial Type 3.pdf
The Rhizobial Type 3.pdf
application/pdf
804279
https://idus.us.es/bitstream/11441/139609/1/The%20Rhizobial%20Type%203.pdf
d48aab548cc8e20b97a5ed6a738c0264
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/139609/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/139609
oai:idus.us.es:11441/139609
2024-02-14 12:15:06.962
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/662422024-02-15T07:48:49Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Galindo Moreno, María
Giráldez Macías, Servando
Sáez, Carmen
Japón Rodríguez, Miguel Ángel
Tortolero García, María Dolores
Romero Portillo, Francisco
2017-11-17T17:03:17Z
2017-11-17T17:03:17Z
2017
Galindo Moreno, M., Giráldez Macías, S., Sáez, C., Japón Rodríguez, M.Á., Tortolero García, M.D. y Romero Portillo, F. (2017). Both p62/SQSTM1-HDAC6-dependent autophagy and the aggresome pathway mediate CDK1 degradation in human breast cancer. Scientific Reports, 7 (10078), 1-10.
2045-2322
http://hdl.handle.net/11441/66242
10.1038/s41598-017-10506-8
Cyclin-dependent kinase 1 (CDK1) is the central mammalian regulator of cell proliferation and a promising therapeutic target for breast cancer. In fact, CDK1 inhibition downregulates survival and induces apoptosis. Due to its essential role, CDK1 expression and activity are strictly controlled at various levels. We previously described that CDK1 stability is also regulated and that SCF(βTrCP) ubiquitinates CDK1, which is degraded via the lysosomal pathway. In addition, in breast tumors from patients, we found a negative correlation between CDK1 accumulation and βTrCP levels, and a positive correlation with the degree of tumor malignancy. This prompted us to study the molecular mechanism involved in CDK1 clearance. In this report, we determine that both chemotherapeutic agents and proteolytic stress induce CDK1 degradation in human breast cancer MCF7 cells through p62/HDAC6-mediated selective autophagy. On the one hand, CDK1 binds to p62/SQSTM1-LC3 and, on the other hand, it interacts with HDAC6. Both complexes are dependent on the presence of an intact βTrCP-binding motif on CDK1. Furthermore, we also show that CDK1 is recruited to aggresomes in response to proteasome inhibition for an extended period. We propose CDK1 clearance as a potential predictive biomarker of antitumor treatment efficacy.
application/pdf
eng
Nature Publishing Group
Scientific Reports, 7 (10078), 1-10.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5577189/pdf/41598_2017_Article_10506.pdf
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Both p62/SQSTM1-HDAC6-dependent autophagy and the aggresome pathway mediate CDK1 degradation in human breast cancer
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Scientific Reports
7
10078
1
10
https://ror.org/03yxnpp24
11 p.
ORIGINAL
pub1articulos novi2017.pdf
pub1articulos novi2017.pdf
application/pdf
1937351
https://idus.us.es/bitstream/11441/66242/1/pub1articulos%20novi2017.pdf
14e52f59864ec78c9059d76f203c78ce
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/66242/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/66242/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/66242
oai:idus.us.es:11441/66242
2024-02-15 08:48:49.314
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1034272024-02-13T19:56:52Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Bernal Guzmán, Patricia
Murillo Torres, Marina
Allsopp, Luke P.
2021-01-05T14:17:27Z
2021-01-05T14:17:27Z
2020
Bernal Guzmán, P., Murillo Torres, M. y Allsopp, L.P. (2020). Integrating signals to drive type VI secretion system killing. Environmental Microbiology, 22 (11), 4520-4523.
1462-2920
https://hdl.handle.net/11441/103427
10.1111/1462-2920.15255
Ministerio de Ciencia, Innovación y Universidades RTI2018-096936-J-I00
application/pdf
4 p.
eng
Society for Applied Microbiology
Environmental Microbiology, 22 (11), 4520-4523.
RTI2018-096936-J-I00
https://doi.org/10.1111/1462-2920.15255
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Integrating signals to drive type VI secretion system killing
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Microbiología
Environmental Microbiology
22
11
4520
4523
https://ror.org/03yxnpp24
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/103427/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
ORIGINAL
10.1111_1462-2920.15255.pdf
10.1111_1462-2920.15255.pdf
Versión postprint
application/pdf
810095
https://idus.us.es/bitstream/11441/103427/3/10.1111_1462-2920.15255.pdf
eaccf970c2f9732ff3d153206d54f617
MD5
3
open access
11441/103427
oai:idus.us.es:11441/103427
2024-02-13 20:56:52.143
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/650142024-02-17T16:42:13Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Martínez Martínez, Luis
Pascual Hernández, Álvaro
Suárez, Ana Isabel
Perea Pérez, Evelio José
2017-10-04T17:03:07Z
2017-10-04T17:03:07Z
1998-12-01
Martínez Martínez, L., Pascual Hernández, Á., Suárez, A.I. y Perea Pérez, E.J. (1998). In vitro activities of ketolide HMR 3647, macrolides, and clindamycin against coryneform bacteria. Antimicrobial Agents and Chemotherapy, 42 (12), 3290-3292.
0066-4804 (impreso)
1098-6596 (electrónico)
http://hdl.handle.net/11441/65014
The in vitro activity of ketolide HMR 3647 against coryneform bacteria isolated from clinical samples was evaluated. Except against Corynebacterium jeikeium and C. urealyticum, HMR 3647 showed high activity against Corynebacterium spp., being more active than 14- and 16-membered macrolides, azithromycin, or clindamycin. HMR 3647 also had high in vitro activity against Brevibacterium spp. and Listeria monocytogenes.
application/pdf
eng
American Society for Microbiology
Antimicrobial Agents and Chemotherapy, 42 (12), 3290-3292.
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
In vitro activities of ketolide HMR 3647, macrolides, and clindamycin against coryneform bacteria
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Antimicrobial Agents and Chemotherapy
42
12
3290
3292
https://ror.org/03yxnpp24
3 p.
ORIGINAL
In vitro activities of ketolide HMR 3647, macrolides, and clindamycin against coryneform bacteria.pdf
In vitro activities of ketolide HMR 3647, macrolides, and clindamycin against coryneform bacteria.pdf
application/pdf
109604
https://idus.us.es/bitstream/11441/65014/1/In%20vitro%20activities%20of%20ketolide%20HMR%203647%2c%20macrolides%2c%20and%20clindamycin%20against%20coryneform%20bacteria.pdf
252f52bd4ea5dc27a5e29d90133db2e0
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/65014/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/65014/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/65014
oai:idus.us.es:11441/65014
2024-02-17 17:42:13.03
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1548532024-02-07T17:55:51Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Pérez Palacios, Patricia
Gual de Torrella, Ana
Portillo Calderón, Inés María
Recacha Villamor, Esther
Franco Álvarez de Luna, Francisco
López Cerero, Lorena
Pascual Hernández, Álvaro
2024-02-07T17:55:51Z
2024-02-07T17:55:51Z
2023
Pérez Palacios, P., Gual de Torrella, A., Portillo Calderón, I.M., Recacha Villamor, E., Franco Álvarez de Luna, F., López Cerero, L. y Pascual Hernández, Á. (2023). Interhospital Spread of blaVIM-1- and blaCTX-M-15-Producing K. pneumoniae ST15 on an IncR Plasmid in Southern Spain. Antibiotics, 12 (12), 1727. https://doi.org/10.3390/antibiotics12121727.
2079-6382
https://hdl.handle.net/11441/154853
10.3390/antibiotics12121727
In 2014–2015, the main CTX-M-15- and OXA-48-producing clone in our region was ST15. Recently, K. pneumoniae ST15 isolates co-producing VIM-1 and CTX-M-15 were detected in several hospitals. The aim was to study the emergence and acquisition of this carbapenemase. Between 2017 and 2019, four hospitals submitted twenty-nine VIM-1- and CTX-M-15-producing K. pneumoniae ST15 isolates to our laboratory. Seven representatives of each XbaI PFGE pulsotype were sequenced using short- and long-read technologies. RAST, CGE databases, and Pathogenwatch were used for resistance determinants and capsule-type analysis. Plasmid comparison was performed with Easyfig2.1. Phylogenetic analysis included other contemporary ST15 isolates from Spain. The 29 isolates were clustered into seven different pulsotypes. The selected genomes, from three hospitals in two different provinces, were clustered together (fewer than 35 alleles) and differed by more than 100 alleles from other ST15 isolates obtained in the region. These seven isolates harbored one IncR plasmid (200–220 kb) with a common backbone and four regions flanked by IS26: one contained blaVIM-1, another contained blaCTX-M-15, the third contained blaOXA-1, and the fourth harbored heavy-metal-tolerance genes. The two initial plasmids, from two different centers, were identical, and rearrangement of four regions was observed in the five subsequent plasmids. Our findings showed the first intercenter dissemination of IncR plasmids carrying blaVIM-1, blaCTX-M-15, and metal-tolerance genes mediated by a new lineage of K. pneumoniae ST15. Two different capture events of the blaVIM-1 gene or different IS26-mediated plasmid rearrangements from a common ancestor may explain plasmid variations.
Centro de Investigación Biomédica en Red de Enfermedades Infecciosas RH-0136-2020
Instituto de Salud Carlos III RH-0136-2020
Junta de Andalucía RH-0136-2020
application/pdf
11 p.
eng
Multidisciplinary Digital Publishing Institute (MDPI)
Antibiotics, 12 (12), 1727.
RH-0136-2020
https://doi.org/10.3390/antibiotics12121727
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Carbapenemase spread
K. pneumoniae high-risk clone
Plasmids
Surveillance
Interhospital Spread of blaVIM-1- and blaCTX-M-15-Producing K. pneumoniae ST15 on an IncR Plasmid in Southern Spain
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Centro de Investigación Biomédica en Red de Enfermedades Infecciosas
Instituto de Salud Carlos III
Junta de Andalucía
Antibiotics
12
12
1727
ORIGINAL
Interhospital Spread.pdf
Interhospital Spread.pdf
application/pdf
1230197
https://idus.us.es/bitstream/11441/154853/1/Interhospital%20Spread.pdf
5e5f578c2dbd844f60032710876d92cd
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1142
https://idus.us.es/bitstream/11441/154853/2/license.txt
4ccdb332745e121f04fdde82917fec0c
MD5
2
open access
11441/154853
oai:idus.us.es:11441/154853
2024-02-07 18:55:51.822
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/233402024-02-14T19:09:17Zcom_11441_10823com_11441_10802com_11441_10690com_11441_10903com_11441_10923col_11441_10824col_11441_10904col_11441_10924
Fedorova, Elena
3e03e8bb-cdc9-4642-9f84-17037cc79e52
-1
Buendía Clavería, Ana María
9129d9c5-0305-4984-84c1-bd4ed9d192bc
-1
Rodríguez Navarro, Dulce Nombre
4a00e0a1-4fd3-42d3-a5b9-6577a938fe86
-1
Ruiz Sainz, José Enrique
468c3f8e-2aa3-4ae0-8d07-0b737a77f9c9
-1
Vinardell González, José María
9862a624-3848-4274-847c-5765940cfa32
-1
Rodríguez Carvajal, Miguel Ángel
9cfe41ea-de6b-427c-a145-45adaaf2fc82
-1
Hidalgo Perea, Ángeles
e28058c7-3230-4931-8400-cdb38b6a78e3
-1
Margaret Oliver, Isabel María
da6e7b5d-c92a-4266-9e12-1aaadd1f66e6
-1
Acosta Jurado, Sebastián
305effbb-de96-4ce8-9794-8cc3614bfe94
-1
Mercedes Luc
72132b18-89ac-4907-8507-2cd85e8afe31
-1
2015-03-03T13:34:48Z
2015-03-03T13:34:48Z
2013
1932-6203
http://www.plosone.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pone.0074717&representation=PDF
http://hdl.handle.net/11441/23340
https://idus.us.es/xmlui/handle/11441/23340
eng
PLoS ONE, 8(10), e74717-e74731
Atribución-NoComercial-SinDerivadas 4.0 España
http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
The Sinorhizobium fredii HH103 Lipopolysaccharide is not only relevant at early soybean nodulation stages but also for symbiosome stability in mature nodules
info:eu-repo/semantics/article
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Química Orgánica
Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular
https://ror.org/03yxnpp24
ORIGINAL
file_1.pdf
application/pdf
3147692
https://idus.us.es/bitstream/11441/23340/1/file_1.pdf
1bce9c186767ac0541022ca38dc93086
MD5
1
open access
11441/23340
oai:idus.us.es:11441/23340
2024-02-14 20:09:17.179
open access
idUS - Universidad de Sevilla
idus@us.es
oai:idus.us.es:11441/384412024-02-14T13:44:02Zcom_11441_11044com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11045col_11441_10904
Rodríguez-Baño, Jesús
Oteo, Jesús
Ortega, Adriana
Villar, Macarena
Conejo Gonzalo, Mª Carmen
Bou, Germán
Aranzamendi-Zaldumbide, Maitane
Cercenado, Emilia
Gurguí, Mercè
Martínez-Martínez, Luis
Merino, María
Rivera, Alba
Oliver, Antonio
Weber, Irene
Pascual Hernández, Álvaro
Bartolomé, Rosa M.
Gónzalez López, Juan José
Campos, José
2016-03-11T18:01:10Z
2016-03-11T18:01:10Z
2013-07
0095-1137
http://hdl.handle.net/11441/38441
http://dx.doi.org/10.1128/JCM.00999-13
https://idus.us.es/xmlui/handle/11441/38441
Two hundred twelve patients with colonization/infection due to amoxicillin-clavulanate (AMC)-resistant Escherichia coli were
studied. OXA-1- and inhibitor-resistant TEM (IRT)-producing strains were associated with urinary tract infections, while
OXA-1 producers and chromosomal AmpC hyperproducers were associated with bacteremic infections. AMC resistance in E.
coli is a complex phenomenon with heterogeneous clinical implications.
application/pdf
eng
American Society for Microbiology
Journal of Clinical Microbiology 51 (7), 2414–2417
http://jcm.asm.org/content/51/7/2414.abstract
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Escherichia coli
Infecciones Urinarias
Infección Hospitalaria
Antibacterianos
Resistencia beta-Lactámica
Estudio Multicéntrico
Epidemiological and Clinical Complexity of Amoxicillin-Clavulanate- Resistant Escherichia coli
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Medicina
Universidad de Sevilla. Departamento de Microbiología
https://ror.org/03yxnpp24
ORIGINAL
20528153.pdf
20528153.pdf
application/pdf
116920
https://idus.us.es/bitstream/11441/38441/1/20528153.pdf
15e321fe1db9ae4cea19bb3bcbf7812e
MD5
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open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/38441/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
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open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/38441/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/38441
oai:idus.us.es:11441/38441
2024-02-14 14:44:02.266
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/610452024-02-13T20:26:32Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Toro, Nicolás
Villadas, PabloJ.
Navarro Gómez, Pilar
Vinardell González, José María
Cuesta Berrio, Lidia
Rodríguez Carvajal, Miguel Ángel
2017-06-06T11:22:30Z
2017-06-06T11:22:30Z
2017
Toro, N., Villadas, P., Navarro Gómez, P., Vinardell González, J.M., Cuesta Berrio, L. y Rodríguez Carvajal, M.Á. (2017). The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population. Scientific Reports, 7 (675), 1-12.
2045-2322
http://hdl.handle.net/11441/61045
10.1038/s41598-017-00730-7
The question of how genotypic and ecological units arise and spread in natural microbial populations remains controversial in the field of evolutionary biology. Here, we investigated the early stages of ecological and genetic differentiation in a highly clonal sympatric Sinorhizobium meliloti population. Whole-genome sequencing revealed that a large DNA region of the symbiotic plasmid pSymB was replaced in some isolates with a similar synteny block carrying densely clustered SNPs and displaying gene acquisition and loss. Two different versions of this genomic island of differentiation (GID) generated by multiple genetic exchanges over time appear to have arisen recently, through recombination in a particular clade within this population. In addition, these isolates display resistance to phages from the same geographic region, probably due to the modification of surface components by the acquired genes. Our results suggest that an underlying process of early ecological and genetic differentiation in S. meliloti is primarily triggered by acquisition of genes that confer resistance to soil phages within particular large genomic DNA regions prone to recombination.
España, Ministerio de Economía y Competitividad BIO 2014-51953-P
application/pdf
eng
Nature Publishing Group
Scientific Reports, 7 (675), 1-12.
info:eu-repo/grantAgreement/MINECO/BIO2014-51953-P
http://dx.doi.org/10.1038/s41598-017-00730-7
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
The underlying process of early ecological and genetic differentiation in a facultative mutualistic Sinorhizobium meliloti population
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Química orgánica
Ministerio de Economía y Competitividad (MINECO). España
Scientific Reports
7
675
1
12
https://ror.org/03yxnpp24
13 p.
ORIGINAL
pub10 41598_2017_Article_730.pdf
pub10 41598_2017_Article_730.pdf
application/pdf
5628121
https://idus.us.es/bitstream/11441/61045/1/pub10%2041598_2017_Article_730.pdf
c5901b2b7459934a0991ee237f4124b6
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/61045/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/61045/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/61045
oai:idus.us.es:11441/61045
2024-02-13 21:26:32.373
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/879282019-09-19T07:29:31Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904oai:idus.us.es:11441/633492024-02-12T21:59:49Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Cantón, Rafael
Loza, Elena
Cornejo Gonzalo, María Carmen
Baquero, Fernando
Martínez Martínez, Luis
2017-07-27T11:48:00Z
2017-07-27T11:48:00Z
2003
Cantón, R., Loza, E., Cornejo Gonzalo, M.C., Baquero, F. y Martínez Martínez, L. (2003). Quality control for β-lactam susceptibility testing with a well-defined collection of Enterobacteriaceae and Pseudomonas aeruginosa strains in Spain. Journal of Clinical Microbiology, 41 (5), 1912-1918.
0095-1137 (impreso)
1098-660X (electronico)
http://hdl.handle.net/11441/63349
10.1128/JCM.41.5.1912-1918.2003
Eighteen Enterobacteriaceae and Pseudomonas aeruginosa strains, 16 of them with well-defined β-lactam re sistance mechanisms, were sent to 52 Spanish microbiology laboratories. Interpretative categories for 8 extended-spectrum β-lactams were collected. Participating laboratories used their own routine susceptibility testing procedures (88% automatic systems, 10% disk diffusion, and 2% agar dilution). Control results were established by two independent reference laboratories by applying the NCCLS microdilution method and interpretative criteria. Interpretative discrepancies were observed in 16% of the results (4.4% for cefepime, 3.0% for aztreonam, 2.8% for piperacillin-tazobactam, 1.7% for cefotaxime [CTX] and ceftazidime, 1.1% for ceftriaxone, 0.9% for meropenem, and 0.3% for imipenem). High consistency with reference values (<5% of major plus very major errors) was observed with (i) American Type Culture Collection quality control strains; (ii) strains with low-efficiency mechanisms inactivating extended-spectrum β-lactams, such as OXA-1-producing Escherichiacoli or SHV-1-hyperproducing Klebsiella pneumoniae; (iii) strains with highly efficient mechanisms, such as SHV-5 porin-deficient K. pneumoniae, CTX-M-10 in Enterobacter cloacae hyperproducing AmpC, and P. aeruginosa with the MexAB OprM efflux phenotype or hyperproducing AmpC. Low consistency (>30% major plus very major errors) was detected in K1-producing Klebsiella oxytoca, CTX-M-9-producing E. coli, and in OprD- P. aeruginosa strains. Extended- spectrum β-lactamase (ESBL) -producing strains accounted for 86% of very major errors. Recognition of the ESBL phenotype was particularly low in Enterobacter cloacae strains (<35%), due to the lack of NCCLS-specific rules in this genus. A K1-producing K. oxytoca was misidentified by 10% of laboratories as an ESBL producer. The use of well-defined resistant strains is useful for improving proficiency in susceptibility testing in clinical laboratories.
application/pdf
eng
American Society for Microbiology
Journal of Clinical Microbiology, 41 (5), 1912-1918.
http://dx.doi.org/10.1128/JCM.41.5.1912-1918.2003
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Quality control for β-lactam susceptibility testing with a well-defined collection of Enterobacteriaceae and Pseudomonas aeruginosa strains in Spain
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Journal of Clinical Microbiology
41
5
1912
1918
https://ror.org/03yxnpp24
7 p.
ORIGINAL
Quality Control for.pdf
Quality Control for.pdf
application/pdf
87325
https://idus.us.es/bitstream/11441/63349/1/Quality%20Control%20for.pdf
0b2d22e9cec0a456723d3f848837b7b5
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/63349/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/63349/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/63349
oai:idus.us.es:11441/63349
2024-02-12 22:59:49.189
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/633402024-02-17T16:20:00Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Petersen, Eskild
Borobio Enciso, María Victoria
Guy, Edward
Liesenfeld, Oliver
Meroni, Valeria
Naessens, Anne
Spranzi, Emma
Thulliez, Philippe
2017-07-27T11:39:08Z
2017-07-27T11:39:08Z
2005
Petersen, E., Borobio Enciso, M.V., Guy, E., Liesenfeld, O., Meroni, V., Naessens, A.,...,Thulliez, P. (2005). European multicenter study of the LIAISON automated diagnostic system for determination of Toxoplasma gondii-specific immunoglobulin G (IgG) and IgM and the IgG avidity index. Journal of Clinical Microbiology, 43 (4), 1570-1574.
0095-1137 (impreso)
1098-660X (electronico)
http://hdl.handle.net/11441/63340
10.1128/JCM.43.4.1570-1574.2005
The LIAISON system is a fully automated system based on chemiluminescence and antigen bound to magnetic microparticles. The system allows fast and precise measurement of Toxoplasma-specific immunoglobulin G (IgG) and IgM antibody levels and measurement of the IgG avidity index even at low levels of Toxoplasma-specific IgG antibodies in a single step without manual interference. Seven European centers participated in a multicenter evaluation of the LIAISON system. The sensitivity and specificity of the LIAISON system compared to the Sabin-Feldman dye test were 99.3 and 96.8%, respectively. In a comparison of the LIAISON Toxoplasma-specific IgM assay with an immunosorbent agglutination assay, the LIAISON assay had a sensitivity of 96.7% and a specificity of 95.4%. The LIAISON IgG assay showed agreements of 91, 100, and 100% with the AXSYM IgG (Abbott), VIDAS IgG (bioMérieux), and Platelia IgG (Bio-Rad) assays, respectively. The LIAISON IgM assay showed agreements of 95% with the AXSYM IgM and Platelia IgM assays, 96% with the ISAGA IgM assay (bioMérieux), and 97% with the VIDAS IgM assay. The coefficient of correlation between the LIAISON system and the VIDAS Toxoplasma-specific IgG avidity index was 0.81. By use of the Toxoplasma-specific IgG avidity index assay with specific IgM-positive samples, the diagnosis of infection with Toxoplasma gondii in early pregnancy has been improved significantly. The LIAISON avidity assay is a valuable assay for the exclusion of recently acquired infection with T. gondii (less than 4 months) in pregnant women, and it decreases significantly the necessity for follow-up testing.
application/pdf
eng
American Society for Microbiology
Journal of Clinical Microbiology, 43 (4), 1570-1574.
http://dx.doi.org/10.1128/JCM.43.4.1570-1574.2005
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
European multicenter study of the LIAISON automated diagnostic system for determination of Toxoplasma gondii-specific immunoglobulin G (IgG) and IgM and the IgG avidity index
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Journal of Clinical Microbiology
43
4
1570
1574
https://ror.org/03yxnpp24
5 p.
ORIGINAL
European Multicenter Study.pdf
European Multicenter Study.pdf
application/pdf
89185
https://idus.us.es/bitstream/11441/63340/1/European%20Multicenter%20Study.pdf
2c9140f80a4111e3a8e1f43c7694dca4
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/63340/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/63340/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/63340
oai:idus.us.es:11441/63340
2024-02-17 17:20:00.395
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1432952024-02-12T21:29:20Zcom_11441_10878com_11441_10802com_11441_10690com_11441_10903col_11441_10879col_11441_10904
Contreras Fernández, Julia Mª
Ruiz Blanco, Óscar
Dominique, Carine
Humbert, Odile
Henry, Yves
Henras, Anthony K.
Cruz Díaz, Jesús de la
Villalobo Polo, Eduardo
2023-03-10T14:28:20Z
2023-03-10T14:28:20Z
2023
Contreras Fernández, J.M., Ruiz Blanco, Ó., Dominique, C., Humbert, O., Henry, Y., Henras, A.K.,...,Villalobo Polo, E. (2023). The Terminal Extensions of Dbp7 Influence Growth and 60S Ribosomal Subunit Biogenesis in Saccharomyces cerevisiae. International Journal of Molecular Sciences, 24 (4), 3460. https://doi.org/10.3390/ijms24043460.
1661-6596
1422-0067
https://hdl.handle.net/11441/143295
10.3390/ijms24043460
Ribosome synthesis is a complex process that involves a large set of protein trans-acting factors, among them DEx(D/H)-box helicases. These are enzymes that carry out remodelling activities onto RNAs by hydrolysing ATP. The nucleolar DEGD-box protein Dbp7 is required for the biogenesis of large 60S ribosomal subunits. Recently, we have shown that Dbp7 is an RNA helicase that regulates the dynamic base-pairing between the snR190 small nucleolar RNA and the precursors of the ribosomal RNA within early pre-60S ribosomal particles. As the rest of DEx(D/H)-box proteins, Dbp7 has a modular organization formed by a helicase core region, which contains conserved motifs, and variable, non-conserved N- and C-terminal extensions. The role of these extensions remains unknown. Herein, we show that the N-terminal domain of Dbp7 is necessary for efficient nuclear import of the protein. Indeed, a basic bipartite nuclear localization signal (NLS) could be identified in its N-terminal domain. Removal of this putative NLS impairs, but does not abolish, Dbp7 nuclear import. Both N- and C-terminal domains are required for normal growth and 60S ribosomal subunit synthesis. Furthermore, we have studied the role of these domains in the association of Dbp7 with pre-ribosomal particles. Altogether, our results show that the N- and C-terminal domains of Dbp7 are important for the optimal function of this protein during ribosome biogenesis.
Ministerio de Ciencia e Innovación PID2019-103850-GB-I00
Junta de Andalucía P20_00581, BIO-271, BIO-210
Universidad de Sevilla US-1380394
application/pdf
21 p.
eng
Multidisciplinary Digital Publishing Institute (MDPI)
International Journal of Molecular Sciences, 24 (4), 3460.
PID2019-103850-GB-I00
P20_00581
BIO-271
BIO-210
US-1380394
https://doi.org/10.3390/ijms24043460
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
60S ribosomal subunit
Dbp7
DEAD-box protein
Ribosome
Ribosome assembly factor
RNA helicase
Saccharomyces cerevisiae
The Terminal Extensions of Dbp7 Influence Growth and 60S Ribosomal Subunit Biogenesis in Saccharomyces cerevisiae
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Genética
Universidad de Sevilla. Departamento de Microbiología
Ministerio de Ciencia e Innovación (MICIN). España
Junta de Andalucía
Universidad de Sevilla
International Journal of Molecular Sciences
24
4
3460
https://ror.org/03yxnpp24
ORIGINAL
The Terminal Extensions.pdf
The Terminal Extensions.pdf
application/pdf
3608393
https://idus.us.es/bitstream/11441/143295/1/The%20Terminal%20Extensions.pdf
ceda013e24bdb73fcfd12792161e1a23
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/143295/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/143295
oai:idus.us.es:11441/143295
2024-02-12 22:29:20.81
open access
idUS - Universidad de Sevilla
idus@us.es
RGVjbGFyYSBxdWU6CgphKSBFcyBhdXRvciB5IHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIHByb3BpZWRhZCBpbnRlbGVjdHVhbC4KCgpiKSBTaSBleGlzdGUgcHJldmlhIGNlc2nDs24gYSB0ZXJjZXJvcyBkZSBsb3MgZGVyZWNob3MgZGUgZXhwbG90YWNpw7NuIGRlIGxhIG9icmEsIGN1ZW50YSBjb24gbGEgYXV0b3JpemFjacOzbiBkZSBkaWNob3MgdGl0dWxhcmVzLiAKCgpjKSBTaSBlbCBkb2N1bWVudG8gY29udGllbmUgbWF0ZXJpYWxlcyBkZSBsb3MgcXVlIG5vIGVzIHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIGV4cGxvdGFjacOzbiwgIHRpZW5lIGVsIHBlcm1pc28gcGFyYSBkZXBvc2l0YXJsb3MgeSBxdWUgZXNlIG1hdGVyaWFsIGVzdMOhIGlkZW50aWZpY2FkbyBjbGFyYW1lbnRlLgoKCkVsIGF1dG9yIHJlYWxpemEgbGEgY2VzacOzbiBncmF0dWl0YSB5IG5vIGV4Y2x1c2l2YSBhIGxhIFVuaXZlcnNpZGFkIGRlIFNldmlsbGEgZGUgbG9zIGRlcmVjaG9zIGRlIHJlcHJvZHVjY2nDs24sIGRpc3RyaWJ1Y2nDs24sIGNvbXVuaWNhY2nDs24gcMO6YmxpY2EgeSB0cmFuc2Zvcm1hY2nDs247IHBlcm1pdGllbmRvIGFsIHJlcG9zaXRvcmlvOgoKCglUcmFuc2Zvcm1hY2nDs24gZGVsIGZvcm1hdG8gcGFyYSBzdSBpbmNvcnBvcmFjacOzbiB5IHByZXNlcnZhY2nDs24uCgoKCVJlcHJvZHVjY2nDs24geSBhcmNoaXZvICBlbiBsb3Mgc2Vydmlkb3JlcyBhc29jaWFkb3MgYWwgcmVwb3NpdG9yaW8uCgoKCVN1IGNvbXVuaWNhY2nDs24gcMO6YmxpY2EgeSBzdSBwdWVzdGEgYSBkaXNwb3NpY2nDs24gZGUgbW9kbyBsaWJyZSB5IGdyYXR1aXRvIGEgdHJhdsOpcyBkZWwgYXJjaGl2byBhYmllcnRvIGluc3RpdHVjaW9uYWwKCgpFbCBhdXRvciBhdXRvcml6YSBxdWUgbGEgb2JyYSBzZSBwb25nYSBhIGRpc3Bvc2ljacOzbiBkZSBsb3MgdXN1YXJpb3MgYSB0cmF2w6lzIGRlbCByZXBvc2l0b3JpbyBpbnN0aXR1Y2lvbmFsIHBhcmEgcXVlIHNlIGhhZ2EgdW4gdXNvIGp1c3RvLCByZXNwZXRhbmRvIGxvcyBkZXJlY2hvcyBkZSBhdXRvciBxdWUgbGEgbGVnaXNsYWNpw7NuIGVzdGFibGVjZSB5IGNvbmZvcm1lIGNvbiBsYXMgY29uZGljaW9uZXMgbWFyY2FkYXMgcG9yIGxhIGxpY2VuY2lhIGRlIHVzbwo=
oai:idus.us.es:11441/620532024-02-14T19:22:04Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Fernández Castillo, Rosario
Rodríguez Valera, Francisco
González Ramos, J.
Ruiz Berraquero, Francisco
2017-07-05T13:07:03Z
2017-07-05T13:07:03Z
1986
Fernández Castillo, R., Rodríguez Valera, F., González Ramos, J. y Ruiz Berraquero, F. (1986). Accumulation of Poly(beta-Hydroxybutyrate) by Halobacteria. Applied and Environmental Microbiology, 51 (1), 214-216.
0099-2240
http://hdl.handle.net/11441/62053
Some species of extremely halophilic archaebacteria, Halobacteriaceae, have been shown to accumulate large amounts of poly(P-hydroxybutyrate) under conditions of nitrogen limitation and abundant carbon source. The production of poly(P3-hydroxybutyrate), at least in large quantities, was restricted to two carbohydrate-utilizing species, Halobacterium mediterranei and H. volcanii. In addition to the nutrients in the media, the salt concentration also influenced poly(P-hydroxybutyrate) accumulation, which was greater at lower salt concentrations. The possible application of these microorganisms for the production of biodegradable plastics is discussed.
Comisión Asesora para el Desarrollo de la Investigación Cientifica y Tecnica
application/pdf
eng
American Society for Microbiology
Applied and Environmental Microbiology, 51 (1), 214-216.
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Halobacterium
Poly(beta hydroxybutyric acid)
Accumulation of Poly(beta-Hydroxybutyrate) by Halobacteria
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Comisión Asesora de Investigación Científica y Técnica (CAICYT). España
Applied and Environmental Microbiology
51
1
214
216
https://ror.org/03yxnpp24
3 p.
ORIGINAL
Accumulation of poly.pdf
Accumulation of poly.pdf
application/pdf
392267
https://idus.us.es/bitstream/11441/62053/1/Accumulation%20of%20poly.pdf
26247cb1f00ec2686dff2ec60f7cc9e6
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/62053/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/62053/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/62053
oai:idus.us.es:11441/62053
2024-02-14 20:22:04.076
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1384362024-02-13T22:08:27Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Martínez-Guijosa, Jordi
López-Alonso, Adrián
Gortázar, Christian
Acevedo, Pelayo
Torres Sánchez, María José
Vicente, Joaquín
2022-10-27T15:25:54Z
2022-10-27T15:25:54Z
2021
Martínez-Guijosa, J., López-Alonso, A., Gortázar, C., Acevedo, P., Torres Sánchez, M.J. y Vicente, J. (2021). Shared use of mineral supplement in extensive farming and its potential for infection transmission at the wildlife-livestock interface. European Journal of Wildlife Research, 67 (3), 55. https://doi.org/10.1007/s10344-021-01493-3.
1612-4642
1439-0574 (electrónico)
https://hdl.handle.net/11441/138436
10.1007/s10344-021-01493-3
Recently, the survival of Mycobacterium bovis on livestock mineral blocks has been confrmed, but little is known about its
implication in the transmission of animal tuberculosis (TB) under feld conditions. The objective of this study was to describe
the shared use of mineral supplements in four extensive beef cattle farms from a high TB prevalence area in South Central
Spain, to identify the main factors explaining their use, and characterize its potential role for the transmission of Mycobac terium tuberculosis Complex (MTC). This is relevant to design control measures at the wildlife-livestock interface. Animal
activity was monitored by camera-trapping at 12 mineral supplementation points during spring and fall. Additionally, swabs
were periodically taken from the mineral substrates and analyzed by PCR searching for MTC DNA. Cattle, pig, goat, sheep,
wild boar, and red deer were all recorded licking on mineral supplementation points. Livestock species were the main users
and presented a diurnal use pattern. Wild ungulates presented a nocturnal-crepuscular use pattern, with scarce overlapping
with livestock. Wild boar presence was positively related to cattle presence at mineral supplementation points, whereas red
deer presence was higher in supplemental points closer to forested areas and in farms without hunting pressure. We recorded
266 indirect wildlife-livestock interactions (i.e., two consecutive visits that occurred within 78 h), all of them derived from
21 unique wildlife visits. All the analyzed swabs resulted negative to MTC DNA. Comparing to other environmental sources
of MTC in our study area, mainly water ponds, this research evidenced that mineral blocks are less attractive to wildlife.
However, the potential for interspecifc transmission of MTC or other pathogens cannot be discarded. The risk for interac tion at mineral supplementation points and further transmission can be prevented by implementing specifc measures in the
context of integral biosecurity plans at the wildlife-livestock interface, which are proposed.
application/pdf
9 p.
eng
Springer
European Journal of Wildlife Research, 67 (3), 55.
https://link.springer.com/article/10.1007/s10344-021-01493-3
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Bovine tuberculosis
Interactions
Interspecifc transmission
Mineral block
Mycobacterium tuberculosis Complex
Photo-trapping
Shared use of mineral supplement in extensive farming and its potential for infection transmission at the wildlife-livestock interface
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. CTS204: Biotecnología Aplicada al Estudio de Enfermedades Infecciosas
European Journal of Wildlife Research
67
3
55
https://ror.org/03yxnpp24
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/138436/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
ORIGINAL
Shared use...pdf
Shared use...pdf
application/pdf
1491833
https://idus.us.es/bitstream/11441/138436/1/Shared%20use...pdf
b567a438c17c16244b4bb44376c990d4
MD5
1
open access
11441/138436
oai:idus.us.es:11441/138436
2024-02-13 23:08:27.604
open access
idUS - Universidad de Sevilla
idus@us.es
RGVjbGFyYSBxdWU6CgphKSBFcyBhdXRvciB5IHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIHByb3BpZWRhZCBpbnRlbGVjdHVhbC4KCgpiKSBTaSBleGlzdGUgcHJldmlhIGNlc2nDs24gYSB0ZXJjZXJvcyBkZSBsb3MgZGVyZWNob3MgZGUgZXhwbG90YWNpw7NuIGRlIGxhIG9icmEsIGN1ZW50YSBjb24gbGEgYXV0b3JpemFjacOzbiBkZSBkaWNob3MgdGl0dWxhcmVzLiAKCgpjKSBTaSBlbCBkb2N1bWVudG8gY29udGllbmUgbWF0ZXJpYWxlcyBkZSBsb3MgcXVlIG5vIGVzIHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIGV4cGxvdGFjacOzbiwgIHRpZW5lIGVsIHBlcm1pc28gcGFyYSBkZXBvc2l0YXJsb3MgeSBxdWUgZXNlIG1hdGVyaWFsIGVzdMOhIGlkZW50aWZpY2FkbyBjbGFyYW1lbnRlLgoKCkVsIGF1dG9yIHJlYWxpemEgbGEgY2VzacOzbiBncmF0dWl0YSB5IG5vIGV4Y2x1c2l2YSBhIGxhIFVuaXZlcnNpZGFkIGRlIFNldmlsbGEgZGUgbG9zIGRlcmVjaG9zIGRlIHJlcHJvZHVjY2nDs24sIGRpc3RyaWJ1Y2nDs24sIGNvbXVuaWNhY2nDs24gcMO6YmxpY2EgeSB0cmFuc2Zvcm1hY2nDs247IHBlcm1pdGllbmRvIGFsIHJlcG9zaXRvcmlvOgoKCglUcmFuc2Zvcm1hY2nDs24gZGVsIGZvcm1hdG8gcGFyYSBzdSBpbmNvcnBvcmFjacOzbiB5IHByZXNlcnZhY2nDs24uCgoKCVJlcHJvZHVjY2nDs24geSBhcmNoaXZvICBlbiBsb3Mgc2Vydmlkb3JlcyBhc29jaWFkb3MgYWwgcmVwb3NpdG9yaW8uCgoKCVN1IGNvbXVuaWNhY2nDs24gcMO6YmxpY2EgeSBzdSBwdWVzdGEgYSBkaXNwb3NpY2nDs24gZGUgbW9kbyBsaWJyZSB5IGdyYXR1aXRvIGEgdHJhdsOpcyBkZWwgYXJjaGl2byBhYmllcnRvIGluc3RpdHVjaW9uYWwKCgpFbCBhdXRvciBhdXRvcml6YSBxdWUgbGEgb2JyYSBzZSBwb25nYSBhIGRpc3Bvc2ljacOzbiBkZSBsb3MgdXN1YXJpb3MgYSB0cmF2w6lzIGRlbCByZXBvc2l0b3JpbyBpbnN0aXR1Y2lvbmFsIHBhcmEgcXVlIHNlIGhhZ2EgdW4gdXNvIGp1c3RvLCByZXNwZXRhbmRvIGxvcyBkZXJlY2hvcyBkZSBhdXRvciBxdWUgbGEgbGVnaXNsYWNpw7NuIGVzdGFibGVjZSB5IGNvbmZvcm1lIGNvbiBsYXMgY29uZGljaW9uZXMgbWFyY2FkYXMgcG9yIGxhIGxpY2VuY2lhIGRlIHVzbwo=
oai:idus.us.es:11441/829542024-02-13T09:15:13Zcom_11441_10903com_11441_10802com_11441_10690com_11441_10913col_11441_10904col_11441_10914
Moyá Morán, María Luisa
López López, Manuel
Lebrón Romero, José Antonio
Ostos Marcos, Francisco José
Camacho Sánchez, Vanesa
Merino Bohórquez, Vicente
López-Cornejo, María del Pilar
2019-02-13T12:09:32Z
2019-02-13T12:09:32Z
2019
Moyá Morán, M.L., López López, M., Lebrón Romero, J.A., Ostos, F.J., Camacho Sánchez, V., Merino Bohórquez, V. y López Cornejo, P. (2019). Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes. Pharmaceutics, 11 (2), 1-12.
1999-4923
https://hdl.handle.net/11441/82954
10.3390/pharmaceutics11020069
Cefepime is an antibiotic with a broad spectrum of antimicrobial activity. However, this antibiotic has several side effects and a high degradation rate. For this reason, the preparation and characterization of new liposomes that are able to encapsulate this antibiotic seem to be an important research line in the pharmaceutical industry. Anionic and cationic liposomes were prepared and characterized. All cationic structures contained the same cationic surfactant, N,N,N-triethyl-N-(12-naphthoxydodecyl)ammonium. Results showed a better encapsulation-efficiency percentage (EE%) of cefepime in liposomes with phosphatidylcholine and cholesterol than with 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE). The presence of cholesterol and the quantity of egg-yolk phospholipid in the liposome increased the encapsulation percentage. The bactericidal activity against Escherichia coli of cefepime loaded into liposomes with phosphatidylcholine was measured. The inhibitory zone in an agar plate for free cefepime was similar to that obtained for loaded cefepime. The growth-rate constant of E. coli culture was also measured in working conditions. The liposome without any antibiotic exerted no influence in such a rate constant. All obtained results suggest that PC:CH:12NBr liposomes are biocompatible nanocarriers of cefepime that can be used in bacterial infections against Escherichia coli with high inhibitory activity.
application/pdf
eng
MDPI
Pharmaceutics, 11 (2), 1-12.
https://doi.org/10.3390/pharmaceutics11020069
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
bactericidal activity
cefepime
encapsulation
Liposome
surfactant
zeta potentia
Preparation and Characterization of New Liposomes. Bactericidal Activity of Cefepime Encapsulated into Cationic Liposomes
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Química Física
Universidad de Sevilla. Departamento de Microbiología
Pharmaceutics
11
2
1
12
https://ror.org/03yxnpp24
12 p.
ORIGINAL
pubpharmaceutics-11-00069.pdf
pubpharmaceutics-11-00069.pdf
application/pdf
2434904
https://idus.us.es/bitstream/11441/82954/1/pubpharmaceutics-11-00069.pdf
516047be4b2038acbce8303b4466a0c4
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/82954/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/82954
oai:idus.us.es:11441/82954
2024-02-13 10:15:13.289
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/609222024-02-17T16:23:44Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Sandal, Niels
Jin, Haojie
Rodríguez Navarro, Dulce Nombre
Ruiz Sainz, José Enrique
2017-06-05T11:39:57Z
2017-06-05T11:39:57Z
2012
Sandal, N., Jin, H., Rodríguez Navarro, D.N. y Ruiz Sainz, J.E. (2012). A Set of Lotus japonicus Gifu × Lotus burttii Recombinant Inbred Lines Facilitates Map-based Cloning and QTL Mapping. DNA Research, 19 (4), 317-323.
1340-2838
http://hdl.handle.net/11441/60922
10.1093/dnares/dss014
Model legumes such as Lotus japonicus have contributed significantly to the understanding of symbiotic nitrogen fixation. This insight is mainly a result of forward genetic screens followed by map-based cloning to identify causal alleles. The L. japonicus ecotype ‘Gifu’ was used as a common parent for inter-accession crosses to produce F2 mapping populations either with other L. japonicus ecotypes, MG-20 and Funakura, or with the related species L. filicaulis. These populations have all been used for genetic studies but segregation distortion, suppression of recombination, low polymorphism levels, and poor viability have also been observed. More recently, the diploid species L. burttii has been identified as a fertile crossing partner of L. japonicus. To assess its qualities in genetic linkage analysis and to enable quantitative trait locus (QTL) mapping for a wider range of traits in Lotus species, we have generated and genotyped a set of 163 Gifu × L. burttii recombinant inbred lines (RILs). By direct comparisons of RIL and F2 population data, we show that L. burttii is a valid alternative to MG-20 as a Gifu mapping partner. In addition, we demonstrate the utility of the Gifu × L. burttii RILs in QTL mapping by identifying an Nfr1-linked QTL for Sinorhizobium fredii nodulation.
application/pdf
eng
Oxford University Press
DNA Research, 19 (4), 317-323.
http://dx.doi.org/10.1093/dnares/dss014
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Lotus japonicus
Lotus burttii
RIL
Sinorhizobium fredii
A Set of Lotus japonicus Gifu × Lotus burttii Recombinant Inbred Lines Facilitates Map-based Cloning and QTL Mapping
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
DNA Research
19
4
317
323
https://ror.org/03yxnpp24
7 p.
ORIGINAL
pub10dss014.pdf
pub10dss014.pdf
application/pdf
374086
https://idus.us.es/bitstream/11441/60922/1/pub10dss014.pdf
d3582cfc44d87f79a59eb004fa86cd5c
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/60922/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/60922/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/60922
oai:idus.us.es:11441/60922
2024-02-17 17:23:44.668
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/648662019-04-03T06:08:40Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Torres Sánchez, María José
Criado, Antonio
Palomares Folía, José Carlos
Aznar Martín, Javier
2017-09-28T17:23:47Z
2017-09-28T17:23:47Z
2000
Torres Sánchez, M.J., Criado, A., Palomares Folia, J.C. y Aznar Martín, J. (2000). Use of real-time PCR and fluorimetry for rapid detection of rifampin and isoniazid resistance-associated mutations in Mycobacterium tuberculosis. Journal of Clinical Microbiology, 38 (9), 3194-3199.
0095-1137 (impreso)
1098-660X (electronico)
http://hdl.handle.net/11441/64866
Very fast amplification of DNA in small volumes can be continuously monitored with a rapid cycler that incorporates fluorimetric detection. Primers were designed to amplify a 157-bp fragment of the rpoB gene spanning codons 526 and 531 and a 209-bp fragment of the katG gene spanning codon 315 of Mycobacterium tuberculosis. Most mutations associated with resistance to rifampin (RMP) and isoniazid (INH) in clinical isolates occur in these codons. Two pairs of hybridization probes were synthesized; one in each pair was 3' labeled with fluorescein and hybridized upstream of the codon with the mutation; the other two probes were 5' labeled with LightCycler-Red 640. Each pair of probes recognized adjacent sequences in the amplicon. After DNA amplification was finished by using a LightCycler, the temperature at which the Red 640 probe melted from the product was determined in a 3-min melt program. Twenty M. tuberculosis clinical isolates susceptible to streptomycin, INH, RMP, and ethambutol and 36 antibiotic-resistant clinical M. tuberculosis isolates (16 resistant to RMP, 16 to INH, and 4 to both antimicrobial agents) were amplified, and the presence of mutations was determined using single-strand conformation polymorphism analysis, the LiQor automated sequencer, and the LightCycler system. Concordant results were obtained in all cases. Within 30 min, the LightCycler method correctly genotyped all the strains without the need of any post-PCR sample manipulation. Overall, this pilot study demonstrated that real-time PCR coupled to fluorescence detection is the fastest available method for the detection of RMP and INH resistance-associated mutations in M. tuberculosis clinical isolates.
Fondo de Investigación Sanitaria, Ministerio de Sanidad y Consumo 99/0269
application/pdf
eng
American Society for Microbiology
Journal of Clinical Microbiology, 38 (9), 3194-3199.
99/0269
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Use of real-time PCR and fluorimetry for rapid detection of rifampin and isoniazid resistance-associated mutations in Mycobacterium tuberculosis
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Ministerio de Sanidad y Consumo. España
Journal of Clinical Microbiology
38
9
3194
3199
6 p.
ORIGINAL
Use of Real-Time.pdf
Use of Real-Time.pdf
application/pdf
101549
https://idus.us.es/bitstream/11441/64866/1/Use%20of%20Real-Time.pdf
97d56dbfbbcf85bfcb13f7e0f5d5ea7d
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/64866/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/64866/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/64866
oai:idus.us.es:11441/64866
2019-04-03 08:08:40.04
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1480992024-02-17T16:26:25Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Brito Santana, Paula
Duque Pedraza, Julián J.
Bernabéu Roda, Lydia M.
Carvia-Hermoso, Cristina
Cuéllar, Virginia
Fuentes Romero, Francisco
Acosta Jurado, Sebastián
Vinardell González, José María
Soto, María J.
2023-07-19T15:09:13Z
2023-07-19T15:09:13Z
2023
Brito Santana, P., Duque Pedraza, J.J., Bernabéu Roda, L.M., Carvia-Hermoso, C., Cuéllar, V., Fuentes Romero, F.,...,Soto, M.J. (2023). Sinorhizobium meliloti DnaJ Is Required for Surface Motility, Stress Tolerance, and for Efficient Nodulation and Symbiotic Nitrogen Fixation. International Journal of Molecular Sciences, 24 (6), 5848. https://doi.org/10.3390/ijms24065848.
1661-6596
1422-0067
https://hdl.handle.net/11441/148099
10.3390/ijms24065848
Bacterial surface motility is a complex microbial trait that contributes to host colonization. However, the knowledge about regulatory mechanisms that control surface translocation in rhizobia and their role in the establishment of symbiosis with legumes is still limited. Recently, 2-tridecanone (2-TDC) was identified as an infochemical in bacteria that hampers microbial colonization of plants. In the alfalfa symbiont Sinorhizobium meliloti, 2-TDC promotes a mode of surface motility that is mostly independent of flagella. To understand the mechanism of action of 2-TDC in S. meliloti and unveil genes putatively involved in plant colonization, Tn5 transposants derived from a flagellaless strain that were impaired in 2-TDC-induced surface spreading were isolated and genetically characterized. In one of the mutants, the gene coding for the chaperone DnaJ was inactivated. Characterization of this transposant and newly obtained flagella-minus and flagella-plus dnaJ deletion mutants revealed that DnaJ is essential for surface translocation, while it plays a minor role in swimming motility. DnaJ loss-of-function reduces salt and oxidative stress tolerance in S. meliloti and hinders the establishment of efficient symbiosis by affecting nodule formation efficiency, cellular infection, and nitrogen fixation. Intriguingly, the lack of DnaJ causes more severe defects in a flagellaless background. This work highlights the role of DnaJ in the free-living and symbiotic lifestyles of S. meliloti.
Ministerio de Ciencia e Innovación PGC2018-096477-B-I00, PID2019-107634R, PID2021-123540NB-I00
application/pdf
21 p.
eng
Multidisciplinary Digital Publishing Institute (MDPI)
International Journal of Molecular Sciences, 24 (6), 5848.
PGC2018-096477-B-I00
PID2019-107634R
PID2021-123540NB-I00
https://doi.org/10.3390/ijms24065848
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Chaperone
Flagella
Nitrogen fixation
Nodulation
Plant colonization
Rhizobium
Stress tolerance
Surface motility
Sinorhizobium meliloti DnaJ Is Required for Surface Motility, Stress Tolerance, and for Efficient Nodulation and Symbiotic Nitrogen Fixation
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Ministerio de Ciencia e Innovación (MICIN). España
International Journal of Molecular Sciences
24
6
5848
https://ror.org/03yxnpp24
ORIGINAL
Sinorhizobium meliloti DnaJ.pdf
Sinorhizobium meliloti DnaJ.pdf
application/pdf
9125934
https://idus.us.es/bitstream/11441/148099/1/Sinorhizobium%20meliloti%20DnaJ.pdf
33d10dd5cec470bc8101c5ab02f02ff5
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1142
https://idus.us.es/bitstream/11441/148099/2/license.txt
4ccdb332745e121f04fdde82917fec0c
MD5
2
open access
11441/148099
oai:idus.us.es:11441/148099
2024-02-17 17:26:25.166
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1457182024-02-13T19:57:16Zcom_11441_11044com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11045col_11441_10904
Vila Domínguez, Andrea
Carretero Ledesma, Marta
Infante Domínguez, Carmen
Cisneros, José Miguel
Lepe Jiménez, José Antonio
Smani, Younes
2023-05-09T12:41:29Z
2023-05-09T12:41:29Z
2023-01-12
Vila Domínguez, A., Carretero Ledesma, M., Infante Domínguez, C., Cisneros, J.M., Lepe Jiménez, J.A. y Smani, Y. (2023). In vitro and in vivo virulence study of Listeria monocytogenes isolated from the Andalusian outbreak in 2019. TROPICAL MEDICINE AND INFECTIOUS DISEASE, 8 (1), 52. https://doi.org/10.3390/tropicalmed8010058.
2414-6366
https://hdl.handle.net/11441/145718
10.3390/tropicalmed8010058
In 2019, the biggest listeriosis outbreak by Listeria monocytogenes (Lm) in the South of Spain
was reported, resulting in the death of three patients from 207 confirmed cases. One strain, belonging
to clonal complex 388 (Lm CC388), has been isolated. We aimed to determine the Lm CC388 virulence
in comparison with other highly virulent clones such as Lm CC1 and Lm CC4, in vitro and in vivo.
Four L. monocytogenes strains (Lm CC388, Lm CC1, Lm CC4 and ATCC 19115) were used. Attachment
to human lung epithelial cells (A549 cells) by these strains was characterized by adherence and
invasion assays. Their cytotoxicities to A549 cells were evaluated by determining the cells viability.
Their hemolysis activity was determined also. A murine intravenous infection model using these
was performed to determine the concentration of bacteria in tissues and blood. Lm CC388 interaction
with A549 cells is non-significantly higher than that of ATCC 19115 and Lm CC1, and lower than that
of Lm CC4. Lm CC388 cytotoxicity is higher than that of ATCC 19115 and Lm CC1, and lower than
that of Lm CC4. Moreover, Lm CC388 hemolysis activity is lower than that of the Lm CC4 strain, and
higher than that of Lm CC1. Finally, in the murine intravenous infection model by Lm CC388, higher
bacterial loads in tissues and at similar levels of Lm CC4 were observed. Although a lower rate of
mortality of patients during the listeriosis outbreak in Spain in 2019 has been reported, the Lm CC388
strain has shown a greater or similar pathogenicity level in vitro and in an animal model, like Lm
CC1 and Lm CC4.
application/pdf
9 p.
eng
MDPI
TROPICAL MEDICINE AND INFECTIOUS DISEASE, 8 (1), 52.
CB21/13/00006
https://www.mdpi.com/2414-6366/8/1/58
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Listeria
Outbreak
Animal model
Virulence
Pathogenicity
In vitro and in vivo virulence study of Listeria monocytogenes isolated from the Andalusian outbreak in 2019
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Medicina
Universidad de Sevilla. Departamento de Microbiología
Consejería de Salud. Junta de Andalucía
TROPICAL MEDICINE AND INFECTIOUS DISEASE
8
1
52
https://ror.org/03yxnpp24
ORIGINAL
In Vitro...pdf
In Vitro...pdf
application/pdf
1771333
https://idus.us.es/bitstream/11441/145718/1/In%20Vitro...pdf
b7aadb55ea5c41bf28cc07ae695cd107
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1138
https://idus.us.es/bitstream/11441/145718/2/license.txt
d037f995682d8bf558422b812ec0f36c
MD5
2
open access
11441/145718
oai:idus.us.es:11441/145718
2024-02-13 20:57:16.37
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/653302024-02-15T07:18:37Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Recacha, E.
Machuca, J.
Díaz Alba, P.
Docobo Pérez, Fernando
Rodríguez Beltrán, Jerónimo
Rodríguez Martínez, José Manuel
2017-10-23T16:28:18Z
2017-10-23T16:28:18Z
2017
Recacha, E., Machuca, J., Díaz Alba, P., Docobo Pérez, F.M., Rodríguez Beltrán, J. y Rodríguez Martínez, J.M. (2017). Quinolone Resistance Reversion by Targeting the SOS Response. mBio, 8 (5), 1-17.
2150-7511
http://hdl.handle.net/11441/65330
10.1128/mBio.00971-17
Suppression of the SOS response has been postulated as a therapeutic strategy for potentiating antimicrobial agents. We aimed to evaluate the impact of its suppression on reversing resistance using a model of isogenic strains of Escherichia coli representing multiple levels of quinolone resistance. E. coli mutants exhibiting a spectrum of SOS activity were constructed from isogenic strains carrying quinolone resistance mechanisms with susceptible and resistant phenotypes. Changes in susceptibility were evaluated by static (MICs) and dynamic (killing curves or flow cytometry) methodologies. A peritoneal sepsis murine model was used to evaluate in vivo impact. Suppression of the SOS response was capable of resensitizing mutant strains with genes encoding three or four different resistance mechanisms (up to 15-fold reductions in MICs). Killing curve assays showed a clear disadvantage for survival (Δlog10 CFU per milliliter [CFU/ml] of 8 log units after 24 h), and the in vivo efficacy of ciprofloxacin was significantly enhanced (Δlog10 CFU/g of 1.76 log units) in resistant strains with a suppressed SOS response. This effect was evident even after short periods (60 min) of exposure. Suppression of the SOS response reverses antimicrobial resistance across a range of E. coli phenotypes from reduced susceptibility to highly resistant, playing a significant role in increasing the in vivo efficacy.
application/pdf
eng
American Society for Microbiology
mBio, 8 (5), 1-17.
http://dx.doi.org/ 10.1128/mBio.00971-17
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
RecA
SOS response
quinolones
resensitization of antibiotic-resistant bacteria
resistance reversion
Quinolone Resistance Reversion by Targeting the SOS Response
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
mBio
8
5
1
17
https://ror.org/03yxnpp24
18 p.
ORIGINAL
pub 1mBio.00971-17.pdf
pub 1mBio.00971-17.pdf
application/pdf
589768
https://idus.us.es/bitstream/11441/65330/1/pub%201mBio.00971-17.pdf
21f36f3873e0c59e11739df822c4efe0
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/65330/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/65330/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/65330
oai:idus.us.es:11441/65330
2024-02-15 08:18:37.535
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/633452024-02-17T17:01:55Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Cueto López, Marina de
Rodríguez Martínez, José Manuel
Soriano Pérez, Manuel Jesús
López Cerero, Lorena
Venero Recio, José Luis
Pascual Hernández, Álvaro
2017-07-27T11:44:37Z
2017-07-27T11:44:37Z
2008
Cueto López, M., Rodríguez Martínez, J.M., Soriano Pérez, M.J., López Cerero, L., Venero, J. y Pascual Hernández, Á. (2008). Fatal levofloxacin failure in treatment of a bacteremic patient infected with Streptococcus pneumoniae with a preexisting parC mutation. Journal of Clinical Microbiology, 46 (4), 1558-1560.
0095-1137 (impreso)
1098-660X (electronico)
http://hdl.handle.net/11441/63345
10.1128/JCM.02066-07
The fatal outcome of levofloxacin treatment in a patient with bacteremic pneumonia caused by Streptococcus pneumoniae with a preexisting parC mutation is reported. Failure was due to the emergence of a gyrA mutation after 4 days of therapy. Problems encountered in detecting first-step mutation isolates are discussed.
Ministerio de Sanidad y Consumo, FEDER y Spanish Network for the Research in Infectious Diseases REIPI RD06/0008
application/pdf
eng
American Society for Microbiology
Journal of Clinical Microbiology, 46 (4), 1558-1560.
REIPI RD06/0008
http://dx.doi.org/10.1128/JCM.02066-07
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Fatal levofloxacin failure in treatment of a bacteremic patient infected with Streptococcus pneumoniae with a preexisting parC mutation
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Ministerio de Sanidad y Consumo. España
European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
Journal of Clinical Microbiology
46
4
1558
1560
https://ror.org/03yxnpp24
3 p.
ORIGINAL
Fatal Levofloxacin.pdf
Fatal Levofloxacin.pdf
application/pdf
90392
https://idus.us.es/bitstream/11441/63345/1/Fatal%20Levofloxacin.pdf
ed0c8484022d27e4c6df586d0f8efa36
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/63345/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/63345/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/63345
oai:idus.us.es:11441/63345
2024-02-17 18:01:55.587
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1454712024-02-14T20:18:38Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Hidalgo-Tenorio, Carmen
Gálvez, Juan
Martínez-Marcos, Francisco Javier
Plata-Ciezar, Antonio
De La Torre-Lima, Javier
López-Cortés, Luis Eduardo
Lepe Jiménez, José Antonio
de Alarcón, Arístides
2023-05-05T11:06:47Z
2023-05-05T11:06:47Z
2020
Hidalgo-Tenorio, C., Gálvez, J., Martínez-Marcos, F.J., Plata-Ciezar, A., De La Torre-Lima, J., López-Cortés, L.E.,...,de Alarcón, A. (2020). Clinical and prognostic differences between methicillin-resistant and methicillin-susceptible Staphylococcus aureus infective endocarditis. BMC Infectious Diseases, 20 (1), 160. https://doi.org/10.1186/s12879-020-4895-1.
1471-2334 (electrónico)
https://hdl.handle.net/11441/145471
10.1186/s12879-020-4895-1
Background: S. aureus (SA) infective endocarditis (IE) has a very high mortality, attributed to the age and comorbidities of patients, inadequate or delayed antibiotic treatment, and methicillin resistance, among other causes. The main study objective was to analyze epidemiological and clinical differences between IE by methicillin-resistant versus methicillin-susceptible SA (MRSA vs. MSSA) and to examine prognostic factors for SA endocarditis, including methicillin resistance and vancomycin minimum inhibitory concentration (MIC) values > 1 μg/mL to MRSA.
Methods: Patients with SA endocarditis were consecutively and prospectively recruited from the Andalusia endocarditis cohort between 1984 and January 2017.
Results: We studied 437 patients with SA endocarditis, which was MRSA in 13.5% of cases. A greater likelihood of history of COPD (OR 3.19; 95% CI 1.41-7.23), invasive procedures, or recognized infection focus in the 3 months before IE onset (OR 2.9; 95% CI 1.14-7.65) and of diagnostic delay (OR 3.94; 95% CI 1.64-9.5) was observed in patients with MRSA versus MSSA endocarditis. The one-year mortality rate due to SA endocarditis was 44.3% and associated with decade of endocarditis onset (1985-1999) (OR 8.391; 95% CI (2.82-24.9); 2000-2009 (OR 6.4; 95% CI 2.92-14.06); active neoplasm (OR 6.63; 95% CI 1.7-25.5) and sepsis (OR 2.28; 95% CI 1.053-4.9). Methicillin resistance was not associated with higher IE-related mortality (49.7 vs. 43.1%; p = 0.32).
Conclusion: MRSA IE is associated with COPD, previous invasive procedure or recognized infection focus, and nosocomial or healthcare-related origin. Methicillin resistance does not appear to be a decisive prognostic factor for SA IE.
application/pdf
11 p.
eng
Biomed Central LTD
BMC Infectious Diseases, 20 (1), 160.
https://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-020-4895-1
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Endocarditis
Methicillin resistance
Staphylococcus aureus
Vancomycin
Clinical and prognostic differences between methicillin-resistant and methicillin-susceptible Staphylococcus aureus infective endocarditis
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
BMC Infectious Diseases
20
1
160
https://ror.org/03yxnpp24
ORIGINAL
Clinical and prognostic differences between methicillin-resistant and methicillin-susceptible Staphylococcus aureus infective endocarditis.pdf
Clinical and prognostic differences between methicillin-resistant and methicillin-susceptible Staphylococcus aureus infective endocarditis.pdf
application/pdf
589261
https://idus.us.es/bitstream/11441/145471/1/Clinical%20and%20prognostic%20differences%20between%20methicillin-resistant%20and%20methicillin-susceptible%20Staphylococcus%20aureus%20infective%20endocarditis.pdf
102d8e61b73840afb84dc6e9a87c3470
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1138
https://idus.us.es/bitstream/11441/145471/2/license.txt
d037f995682d8bf558422b812ec0f36c
MD5
2
open access
11441/145471
oai:idus.us.es:11441/145471
2024-02-14 21:18:38.852
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/688122024-02-13T22:09:40Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Jiménez Guerrero, Irene
Acosta Jurado, Sebastián
Cerro Sánchez, Pablo del
Navarro Gómez, Pilar
López Baena, Francisco Javier
Ollero Márquez, Francisco Javier
Vinardell González, José María
Pérez Montaño, Francisco de Asís
2018-01-11T17:14:24Z
2018-01-11T17:14:24Z
2018
Jiménez Guerrero, I., Acosta Jurado, S., Cerro Sánchez, P.d., Navarro Gómez, P., López Baena, F.J., Ollero Márquez, F.J.,...,Pérez Montaño, F.d.A. (2018). Transcriptomic studies of the effect of nod gene-inducing molecules in rhizobia: Different weapons, one purpose. Genes, 9 (1), 1-.
2073-4425
http://hdl.handle.net/11441/68812
10.3390/genes9010001
Simultaneous quantification of transcripts of the whole bacterial genome allows the analysis of the global transcriptional response under changing conditions. RNA-seq and microarrays are the most used techniques to measure these transcriptomic changes, and both complement each other in transcriptome profiling. In this review, we exhaustively compiled the symbiosis-related transcriptomic reports (microarrays and RNA sequencing) carried out hitherto in rhizobia. This review is specially focused on transcriptomic changes that takes place when five rhizobial species, Bradyrhizobium japonicum (=diazoefficiens) USDA 110, Rhizobium leguminosarum biovar viciae 3841, Rhizobium tropici CIAT 899, Sinorhizobium (=Ensifer) meliloti 1021 and S. fredii HH103, recognize inducing flavonoids, plant-exuded phenolic compounds that activate the biosynthesis and export of Nod factors (NF) in all analysed rhizobia. Interestingly, our global transcriptomic comparison also indicates that each rhizobial species possesses its own arsenal of molecular weapons accompanying the set of NF in order to establish a successful interaction with host legumes.
Ministerio de Economía y Competitividad BIO2016-78409-R, AGL2016-77163-R
application/pdf
eng
Multidisciplinary Digital Publishing Institute (MDPI)
Genes, 9 (1), 1-.
BIO2016-78409-R
AGL2016-77163-R
http://dx.doi.org/10.3390/genes9010001
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Flavonoids
Microarray
Nodulation
Rhizobia
RNA-seq
Symbiosis
Transcriptome
Transcriptomic studies of the effect of nod gene-inducing molecules in rhizobia: Different weapons, one purpose
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Ministerio de Economía y Competitividad (MINECO). España
Genes
9
1
1
https://ror.org/03yxnpp24
27 p.
ORIGINAL
Transcriptomic Studies of the Effect.pdf
Transcriptomic Studies of the Effect.pdf
application/pdf
3474601
https://idus.us.es/bitstream/11441/68812/1/Transcriptomic%20Studies%20of%20the%20Effect.pdf
9f575c61937e719b93c65fa35dd40090
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/68812/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/68812/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/68812
oai:idus.us.es:11441/68812
2024-02-13 23:09:40.756
open access
idUS - Universidad de Sevilla
idus@us.es
RGVjbGFyYSBxdWU6CgphKSBFcyBhdXRvciB5IHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIHByb3BpZWRhZCBpbnRlbGVjdHVhbC4KCgpiKSBTaSBleGlzdGUgcHJldmlhIGNlc2nDs24gYSB0ZXJjZXJvcyBkZSBsb3MgZGVyZWNob3MgZGUgZXhwbG90YWNpw7NuIGRlIGxhIG9icmEsIGN1ZW50YSBjb24gbGEgYXV0b3JpemFjacOzbiBkZSBkaWNob3MgdGl0dWxhcmVzLiAKCgpjKSBTaSBlbCBkb2N1bWVudG8gY29udGllbmUgbWF0ZXJpYWxlcyBkZSBsb3MgcXVlIG5vIGVzIHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIGV4cGxvdGFjacOzbiwgIHRpZW5lIGVsIHBlcm1pc28gcGFyYSBkZXBvc2l0YXJsb3MgeSBxdWUgZXNlIG1hdGVyaWFsIGVzdMOhIGlkZW50aWZpY2FkbyBjbGFyYW1lbnRlLgoKCkVsIGF1dG9yIHJlYWxpemEgbGEgY2VzacOzbiBncmF0dWl0YSB5IG5vIGV4Y2x1c2l2YSBhIGxhIFVuaXZlcnNpZGFkIGRlIFNldmlsbGEgZGUgbG9zIGRlcmVjaG9zIGRlIHJlcHJvZHVjY2nDs24sIGRpc3RyaWJ1Y2nDs24sIGNvbXVuaWNhY2nDs24gcMO6YmxpY2EgeSB0cmFuc2Zvcm1hY2nDs247IHBlcm1pdGllbmRvIGFsIHJlcG9zaXRvcmlvOgoKCglUcmFuc2Zvcm1hY2nDs24gZGVsIGZvcm1hdG8gcGFyYSBzdSBpbmNvcnBvcmFjacOzbiB5IHByZXNlcnZhY2nDs24uCgoKCVJlcHJvZHVjY2nDs24geSBhcmNoaXZvICBlbiBsb3Mgc2Vydmlkb3JlcyBhc29jaWFkb3MgYWwgcmVwb3NpdG9yaW8uCgoKCVN1IGNvbXVuaWNhY2nDs24gcMO6YmxpY2EgeSBzdSBwdWVzdGEgYSBkaXNwb3NpY2nDs24gZGUgbW9kbyBsaWJyZSB5IGdyYXR1aXRvIGEgdHJhdsOpcyBkZWwgYXJjaGl2byBhYmllcnRvIGluc3RpdHVjaW9uYWwKCgpFbCBhdXRvciBhdXRvcml6YSBxdWUgbGEgb2JyYSBzZSBwb25nYSBhIGRpc3Bvc2ljacOzbiBkZSBsb3MgdXN1YXJpb3MgYSB0cmF2w6lzIGRlbCByZXBvc2l0b3JpbyBpbnN0aXR1Y2lvbmFsIHBhcmEgcXVlIHNlIGhhZ2EgdW4gdXNvIGp1c3RvLCByZXNwZXRhbmRvIGxvcyBkZXJlY2hvcyBkZSBhdXRvciBxdWUgbGEgbGVnaXNsYWNpw7NuIGVzdGFibGVjZSB5IGNvbmZvcm1lIGNvbiBsYXMgY29uZGljaW9uZXMgbWFyY2FkYXMgcG9yIGxhIGxpY2VuY2lhIGRlIHVzbwo=
oai:idus.us.es:11441/411272024-02-13T21:56:32Zcom_11441_11044com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11045col_11441_10904
Rodríguez-Baño, Jesús
Dolores Navarro, María
Romero, Luisa
Martínez-Martínez, Luis
Muniain Ezcurra, Miguel Angel
Perea J., Evelio
Pérez Cano, Ramón
Pascual Hernández, Álvaro
2016-05-12T17:54:34Z
2016-05-12T17:54:34Z
2004-03
0095-1137
http://hdl.handle.net/11441/41127
http://dx.doi.org/10.1128/jcm.42.3.1089-1094.2004
https://idus.us.es/xmlui/handle/11441/41127
Infections due to extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli (ESBLEC) in nonhospitalize patients seem to be emerging in different countries. Their incidence, epidemiology, and clinical impact in the community have not been studied. We describe the epidemiology and clinical features of infections caused by ESBLEC in nonhospitalized patients in Spain and the results of a case-control study performed to investigate the risk factors associated with the acquisition of these organisms. The clonal
relatedness of the organisms was assessed by repetitive extragenic palindromic sequence PCR. The ESBLs and the genes encoding the ESBLs were initially characterized by isoelectric focusing and PCR, respectively.
Forty-nine patients (76% with urinary tract infections, 22% with asymptomatic bacteriuria, and 2% with acute cholangitis) were included. Six patients were bacteremic. Diabetes mellitus (odds ratio, 5.5; 95% confidence
interval, 1.6 to 18.7), previous fluoroquinolone use (odds ratio, 7.6; 95% confidence interval, 1.9 to 30.1), recurrent urinary tract infections (odds ratio, 4.5; 95% confidence interval, 1.3 to 15.1), a previous hospital
admission (odds ratio, 18.2; 95% confidence interval, 5.3 to 61.1), and older age in male patients (odds ratio per year, 1.03; 95% confidence interval, 1.03 to 1.05) were identified as risk factors by multivariate analysis. The
ESBLEC isolates were not clonally related. The ESBLs were characterized as members of the CTX-M-9 group, the SHV group, and the TEM group in 64, 18, and 18% of the isolates, respectively. ESBLEC is an emergent
cause of urinary tract infections in nonhospitalized patients. There was no evidence of horizontal transmission of ESBLEC strains. Avoidance of fluoroquinolone use in high-risk patients should be considered whenever
possible in order to avoid the selection of these organisms.
application/pdf
eng
American Society for Microbiology
Journal of Clinical Microbiology, 42(3), 1089–1094
http://dx.doi.org/10.1128/jcm.42.3.1089-1094.2004
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Epidemiology and Clinical Features of Infections Caused by Extended-Spectrum Beta-Lactamase-Producing Escherichia coli in Nonhospitalized Patients
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Medicina
Universidad de Sevilla. Departamento de Microbiología
https://ror.org/03yxnpp24
6 p.
ORIGINAL
6680725.pdf
6680725.pdf
application/pdf
98935
https://idus.us.es/bitstream/11441/41127/1/6680725.pdf
bb1fb4ca974df639113d60f2a6c6eb3d
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/41127/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/41127/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/41127
oai:idus.us.es:11441/41127
2024-02-13 22:56:32.441
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1382912024-01-29T17:22:45Zcom_11441_11044com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11045col_11441_10904
Universidad de Sevilla. Departamento de Medicina
Universidad de Sevilla. Departamento de Microbiología
Gutiérrez Gutiérrez, Belén
Pérez-Nadales, Elena
Pérez-Galera, Salvador
Cordero Matia, María Elisa
Pascual Hernández, Álvaro
Rodríguez-Baño, Jesús
2022-10-24T16:46:21Z
2022-10-24T16:46:21Z
2021
Gutiérrez Gutiérrez, B., Pérez-Nadales, E., Pérez-Galera, S., Cordero Matia, M.E., Pascual Hernández, Á. y Rodríguez-Baño, J. (2021). Propensity score and desirability of outcome ranking analysis of ertapenem for treatment of nonsevere bacteremic urinary tract infections due to extended-spectrum-beta-lactamase-producing enterobacterales in kidney transplant recipients. Antimicrobial Agents and Chemotherapy, 65 (11), e01102-21. https://doi.org/10.1128/AAC.01102-21.
0066-4804
1098-6596
https://hdl.handle.net/11441/138291
10.1128/AAC.01102-21
There are scarce data on the efficacy of ertapenem in the treatment of bacteremia due to extended-spectrum-beta-lactamase (ESBL)-producing Enterobacterales (ESBL-E) in kidney transplant (KT) recipients. We evaluated the association between treatment with ertapenem or meropenem and clinical cure in KT recipients with nonsevere bacteremic urinary tract infections (B-UTI) caused by ESBL-E. We performed a registered, retrospective, international (29 centers in 14 countries) cohort study (INCREMENT-SOT, NCT02852902). The association between targeted therapy with ertapenem versus meropenem and clinical cure at day 14 (the principal outcome) was studied by logistic regression. Propensity score matching and desirability of outcome ranking (DOOR) analyses were also performed. A total of 201 patients were included; only 1 patient (treated with meropenem) in the cohort died. Clinical cure at day 14 was reached in 45/100 (45%) and 51/101 (50.5%) of patients treated with ertapenem and meropenem, respectively (adjusted OR 1.29; 95% CI 0.51 to 3.22; P = 0.76); the propensity score-matched cohort included 55 pairs (adjusted OR for clinical cure at day 14, 1.18; 95% CI 0.43 to 3.29; P = 0.74). In this cohort, the proportion of cases treated with ertapenem with better DOOR than with meropenem was 49.7% (95% CI, 40.4 to 59.1%) when hospital stay was considered. It ranged from 59 to 67% in different scenarios of a modified (weights-based) DOOR sensitivity analysis when potential ecological advantage or cost was considered in addition to outcome. In conclusion, targeted therapy with ertapenem appears as effective as meropenem to treat nonsevere B-UTI due to ESBL-E in KT recipients and may have some advantages.
Instituto de Salud Carlos III RD16/0016/0001
Ministerio de Ciencias e Innovación PI18/01849
application/pdf
13 p.
eng
American Society Microbiology
Antimicrobial Agents and Chemotherapy, 65 (11), e01102-21.
http://doi.org/10.1128/AAC.01102-21
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Ertapenem
Urinary tract infections
Enterobacterales
Spectrum-beta-lactamase
Kidney transplant
Propensity score and desirability of outcome ranking analysis of ertapenem for treatment of nonsevere bacteremic urinary tract infections due to extended-spectrum-beta-lactamase-producing enterobacterales in kidney transplant recipients
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Antimicrobial Agents and Chemotherapy
65
11
e01102-21
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/138291/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
ORIGINAL
336.pdf
336.pdf
application/pdf
563253
https://idus.us.es/bitstream/11441/138291/1/336.pdf
5e63f3b2de404dc95d945211520c5db9
MD5
1
open access
11441/138291
oai:idus.us.es:11441/138291
2024-01-29 18:22:45.139
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/648612024-02-14T09:18:38Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Martínez Martínez, Luis
2017-09-28T17:08:31Z
2017-09-28T17:08:31Z
1995
Martínez Martínez, L. (1995). Comparison of E-test with broth microdilution and disk diffusion for susceptibility testing of coryneform bacteria. Journal of Clinical Microbiology, 33 (5), 1318-1321.
0095-1137 (impreso)
1098-660X (electronico)
http://hdl.handle.net/11441/64861
The susceptibilities of 135 coryneform bacteria isolated from clinical samples to ampicillin (AMP), cephalothin (CR), cefoxitin (FOX), cefotaxime (CTX), erythromycin (E), ciprofioxacin (CIP), tetracycline (TE), amikacin (AK), vancomycin (VA), and rifampin (R) were determined by disk diffusion, broth microdilution, and the E-test. The following species (number of isolates in parentheses) were included: Corynebacterium urealyticum (30), Corynebacterium minutissimum (20), coryneform CDC group ANF-1 (20), Corynebacterium striatum (20), Corynebacterium jeikeium (15), coryneform CDC group 12 (8), Listeria monocytogenes (7), Corynebacterium xerosis (5), and other coryneform bacteria (10). Agreement within one twofold dilution between the E-test and broth microdilution was 31% (VA), 64% (AK), 71% (CTX), 77% (FOX and CIP), 79% (TE), 84% (AMP), 87% (E), and 88% (CR and R). For the 1,350 combinations of microorganisms and antimicrobial agents, 85 (6.3%) discrepancies in interpretive category were found (4.2% minor, 1.2% major, and 0.9% very major). Seventy (5.1%) disagreements in interpretive category were found between disk diffusion and the E-test (3.8% minor, 0.4% major, and 0.9% very major), and 85 (6.3%) disagreements were found between microdilution (reference method) and disk diffusion (4.2% minor, 0.5% major, and 1.5% very major). MICs obtained with the E-test were highly reproducible. No category discrepancy was observed for VA, despite quantitative results. Considering interpretive categories, there is a good overall agreement between the three methods studied here, but further evaluation of current methodologies for susceptibility testing is required when considering coryneform bacteria and determination of quantitative activity of antimicrobial agents.
application/pdf
eng
American Society for Microbiology
Journal of Clinical Microbiology, 33 (5), 1318-1321.
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Comparison of E-test with broth microdilution and disk diffusion for susceptibility testing of coryneform bacteria
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Journal of Clinical Microbiology
33
5
1318
1321
https://ror.org/03yxnpp24
4 p.
ORIGINAL
Comparison of E-test.pdf
Comparison of E-test.pdf
application/pdf
154760
https://idus.us.es/bitstream/11441/64861/1/Comparison%20of%20E-test.pdf
dbd6c4fa24575b6c8a1a63324be568f4
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/64861/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/64861/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/64861
oai:idus.us.es:11441/64861
2024-02-14 10:18:38.757
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1033652020-12-18T19:01:42Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Bleriot, Inés
Blasco, L.
Delgado Valverde, María Mercedes
Gual-de-Torrella, Ana
Ambroa, A.
Fernandez-Garcia, Laura
Pascual Hernández, Álvaro
Tomas, Maria
2020-12-18T19:01:09Z
2020-12-18T19:01:09Z
2020-09-02
Bleriot, I., Blasco, L., Delgado Valverde, M.M., Gual-de-Torrella, A., Ambroa, A., Fernandez-Garcia, L.,...,Tomas, M. (2020). Mechanisms of Tolerance and Resistance to Chlorhexidine in Clinical Strains of Klebsiella pneumoniae Producers of Carbapenemase: Role of New Type II Toxin-Antitoxin System, PemIK. Toxins, 12 (9), 1-17.
2072-6651
https://hdl.handle.net/11441/103365
10.3390/toxins12090566
Although the failure of antibiotic treatment is normally attributed to resistance, tolerance
and persistence display a significant role in the lack of response to antibiotics. Due to the fact that
several nosocomial pathogens show a high level of tolerance and/or resistance to chlorhexidine,
in this study we analyzed the molecular mechanisms associated with chlorhexidine adaptation in two
clinical strains of Klebsiella pneumoniae by phenotypic and transcriptomic studies. These two strains
belong to ST258-KPC3 (high-risk clone carrying β-lactamase KPC3) and ST846-OXA48 (low-risk
clone carrying β-lactamase OXA48). Our results showed that the K. pneumoniae ST258-KPC3CA
and ST846-OXA48CA strains exhibited a different behavior under chlorhexidine (CHLX) pressure,
adapting to this biocide through resistance and tolerance mechanisms, respectively. Furthermore,
the appearance of cross-resistance to colistin was observed in the ST846-OXA48CA strain (tolerant to
CHLX), using the broth microdilution method. Interestingly, this ST846-OXA48CA isolate contained
a plasmid that encodes a novel type II toxin/antitoxin (TA) system, PemI/PemK. We characterized this
PemI/PemK TA system by cloning both genes into the IPTG-inducible pCA24N plasmid, and found
their role in persistence and biofilm formation. Accordingly, the ST846-OXA48CA strain showed
a persistence biphasic curve in the presence of a chlorhexidine-imipenem combination, and these
results were confirmed by the enzymatic assay (WST-1).
The State Plan for R+D+I 2013–2016 National Plan for Scientific Research, Technological Development and Innovation 2008–2011 PI16/01163 and PI19/00878
ISCIII-Deputy General Directorate for Evaluation and Promotion of Research - European Regional Development Fund “A way of Making Europe” and Instituto de Salud Carlos III FEDER, Spanish Network for the Research in Infectious Diseases REIPI, RD16/0016/0001, RD16/CIII/0004/0002 and RD16/0016/0006
The Study Group on Mechanisms of Action and Resistance to Antimicrobials, GEMARA
application/pdf
17
eng
MDPI
Toxins
PI16/01163
PI19/00878
RD16/0016/0001
RD16/CIII/0004/0002
RD16/0016/0006
https://doi.org/10.3390/toxins12090566
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Tolerance
Persistence
Cross-resistance
Toxin-antitoxin system
PemI/PemK
Klebsiella pneumoniae
Mechanisms of Tolerance and Resistance to Chlorhexidine in Clinical Strains of Klebsiella pneumoniae Producers of Carbapenemase: Role of New Type II Toxin-Antitoxin System, PemIK
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Toxins
12
9
1
17
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/103365/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
ORIGINAL
toxins-12-00566-v2.pdf
toxins-12-00566-v2.pdf
Mechanisms of Tolerance
application/pdf
1756804
https://idus.us.es/bitstream/11441/103365/1/toxins-12-00566-v2.pdf
88bbaff29a84a1c656b6f44cd026e56d
MD5
1
open access
11441/103365
oai:idus.us.es:11441/103365
2020-12-18 20:01:42.088
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1410952024-02-14T20:16:15Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Fernández-Cuenca, Felipe
López Hernández, Inmaculada
Cercenado, Emilia
Conejo Gonzalo, Mª Carmen
Tormo, Nuria
Gimeno, Concha
Pascual Hernández, Álvaro
2023-01-10T15:18:33Z
2023-01-10T15:18:33Z
2021-02-08
Fernández-Cuenca, F., López Hernández, I., Cercenado, E., Conejo Gonzalo, M.C., Tormo, N., Gimeno, C. y Pascual Hernández, Á. (2021). Reporting antimicrobial susceptibilities and resistance phenotypes in Staphylococcus spp.: a nationwide proficiency study. Journal of Antimicrobial Chemotherapy, 76 (5), 1187-1196. https://doi.org/10.1093/jac/dkab017.
0305-7453;1460-2091
https://hdl.handle.net/11441/141095
10.1093/jac/dkab017
Objectives
To evaluate the proficiency of microbiology laboratories in Spain in antimicrobial susceptibility testing (AST) of Staphylococcus spp.
Materials and methods
Eight Staphylococcus spp. with different resistance mechanisms were selected: six Staphylococcus aureus (CC-01/mecA, CC-02/mecC, CC-03/BORSA, CC-04/MLSBi, CC-06/blaZ and CC-07/linezolid resistant, cfr); one Staphylococcus epidermidis (CC-05/linezolid resistant, 23S rRNA mutation); and one Staphylococcus capitis (CC-08/daptomycin non-susceptible). Fifty-one laboratories were asked to report: (i) AST system used; (ii) antimicrobial MICs; (iii) breakpoints used (CLSI or EUCAST); and (iv) clinical category. Minor, major and very major errors (mEs, MEs and VMEs, respectively) were determined.
Results
The greatest MIC discrepancies found were: (i) by AST method: 19.4% (gradient diffusion); (ii) by antimicrobial agent: daptomycin (21.3%) and oxacillin (20.6%); and (iii) by isolate: CC-07/cfr (48.0%). The greatest error rates were: (i) by AST method: gradient diffusion (4.3% and 5.1% VMEs, using EUCAST and CLSI, respectively); (ii) by breakpoint: 3.8% EUCAST and 2.3% CLSI; (iii) by error type: mEs (0.8% EUCAST and 1.0% CLSI), MEs (1.8% EUCAST and 0.7% CLSI) and VMEs (1.2% EUCAST and 0.6% CLSI); (iii) by antimicrobial agent: VMEs (4.7% linezolid and 4.3% oxacillin using EUCAST); MEs (14.3% fosfomycin, 9.1% tobramycin and 5.7% gentamicin using EUCAST); and mEs (22.6% amikacin using EUCAST).
Conclusions
Clinical microbiology laboratories should improve their ability to determine the susceptibility of Staphylococcus spp. to some antimicrobial agents to avoid reporting false-susceptible or false-resistant results. The greatest discrepancies and errors were associated with gradient diffusion, EUCAST breakpoints and some antimicrobials (mEs for aminoglycosides; MEs for fosfomycin, aminoglycosides and oxacillin; and VMEs for linezolid and oxacillin).
application/pdf
10 p.
eng
Oxford University Press
Journal of Antimicrobial Chemotherapy, 76 (5), 1187-1196.
RD 16/0016
https://academic.oup.com/jac/article/76/5/1187/6130710
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
phenotype
gentamicin sulfate (usp)
amikacin
diffusion
chromatography
micellar electrokinetic capillary
daptomycin
fosfomycin
gentamicins
laboratory
oxacillin
staphylococcus
microbiology
tobramycin
linezolid
antimicrobials
chief complaint
malnutrition-inflammation-cachexia syndrome
Reporting antimicrobial susceptibilities and resistance phenotypes in Staphylococcus spp.: a nationwide proficiency study
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Instituto de Salud Carlos III
Journal of Antimicrobial Chemotherapy
76
5
1187
1196
https://ror.org/03yxnpp24
ORIGINAL
Reporting antimicrobial susceptibilities and resistance phenotypes in Staphylococcus spp. a nationwide proficiency study.pdf
Reporting antimicrobial susceptibilities and resistance phenotypes in Staphylococcus spp. a nationwide proficiency study.pdf
application/pdf
344880
https://idus.us.es/bitstream/11441/141095/1/Reporting%20antimicrobial%20susceptibilities%20and%20resistance%20phenotypes%20in%20Staphylococcus%20spp.%20a%20nationwide%20proficiency%20study.pdf
8ab4f6c6fd0e3c640d46f587f567992b
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/141095/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/141095
oai:idus.us.es:11441/141095
2024-02-14 21:16:15.926
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1544282024-02-01T13:59:20Zcom_11441_11094com_11441_10983com_11441_10690com_11441_11044com_11441_10903com_11441_10802col_11441_11095col_11441_11045col_11441_10904
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Medicina
Universidad de Sevilla. Instituto de Biomedicina de Sevilla (IBIS)
EFPIA
European Development Regional Fund
European Union
Fondo de Investigacion Sanitaria
Industria y Competitividad
Innovative Medicines Initiative
Instituto de Salud Carlos III
Junta de Andalucia
Ministerio de Economia
Operative Programme INtelligent Growth
Plan Nacional de I+D+i
Spanish Network for Research in Infectious Diseases
Subdireccion General de Redes y Centros de Investigacion Cooperativa
Torres, E.
López Cerero, Lorena
Navarro, M. D.
Rodríguez-Baño, Jesús
Pascual Hernández, Álvaro
2024-02-01T13:45:10Z
2024-02-01T13:45:10Z
2018
Torres, E., López Cerero, L., Navarro, M.D., Rodríguez-Baño, J. y Pascual Hernández, Á. (2018). Prevalence and transmission dynamics of Escherichia coli ST131 among contacts of infected community and hospitalized patients. Clinical Microbiology and Infection (CMI), 24 (6), 618-623. https://doi.org/10.1016/j.cmi.2017.09.007.
1198-743X
1469-0691
https://hdl.handle.net/11441/154428
10.1016/j.cmi.2017.09.007
Objectives: The Escherichia coli O25b-associated ST131 clonal group was recently found to be prevalent in our area as a cause of community-acquired urinary tract infections. We evaluated the transmission dynamics and longitudinal persistence of E. coli O25b-ST131 between patients with nosocomial and community-acquired infections and their contacts.
Methods: Prevalence and transmission of O25b/pabB3/B23 isolates were compared in 38 community clusters, 30 nosocomial clusters and 50 healthy volunteers. Duration of colonization was studied at 1 to 4 months and 6 to 12 months after the first sample. Isolates exhibiting a three-band or less difference by pulsed-field gel electrophoresis were assigned to the same pulsotype.
Results: Colonization was found to be more frequent in index cases (31/68, 45.6%) than in contacts (25/118, 21.2%; p 0.0009) or volunteers (1/50, 2%; p 0.0009). Seven (11%) of 64 isolates were extended-spectrum β-lactamase producers. Transmission occurred in 61% (8/13) community clusters and in 12% (1/8) nosocomial clusters. Thirteen (56.5%) of the 23 initial carriers assessed at 1 to 4 months remained colonized. Only 2 (13.3%) of 15 positive patients followed for 6 to 12 months showed prolonged carriage, and none was infected with extended-spectrum β-lactamase producers. Six previously positive individuals acquired a different ST131 pulsotype (5/23 at sample 2 and 1/15 at sample 3), and three previously negative individuals became positive (2/46 at 1-4 months and 1/33 at 6-12 months).
Conclusions: Person-to-person transmission or acquisition from a common source of E. coli O25b-associated ST131 is more frequent in the household setting than in the nosocomial setting. The carrier state does not usually last beyond 4 months, with new acquisitions in certain individuals.
application/pdf
6 p.
eng
Elsevier
Clinical Microbiology and Infection (CMI), 24 (6), 618-623.
115523
115620
115737
070190
10/02021
10/01955
10/00795
0048/2008
CTX-5259
CTS210
REIPI RD12/0015/0010
REIPI RD16/0016/0001
https://www.sciencedirect.com/science/article/pii/S1198743X17305050?via%3Dihub
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Escherichia coli
Horizontal transmission
Intestinal carriage
Intestinal persistence
ST131
Prevalence and transmission dynamics of Escherichia coli ST131 among contacts of infected community and hospitalized patients
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Clinical Microbiology and Infection (CMI)
24
6
618
623
ORIGINAL
Prevalence and transmission dynamics of Escherichia coli ST131 among contacts of infected community and hospitalized patients.pdf
Prevalence and transmission dynamics of Escherichia coli ST131 among contacts of infected community and hospitalized patients.pdf
application/pdf
805630
https://idus.us.es/bitstream/11441/154428/1/Prevalence%20and%20transmission%20dynamics%20of%20Escherichia%20coli%20ST131%20among%20contacts%20of%20infected%20community%20and%20hospitalized%20patients.pdf
c4cdd5808efbb732d7a610f96c259158
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1142
https://idus.us.es/bitstream/11441/154428/2/license.txt
4ccdb332745e121f04fdde82917fec0c
MD5
2
open access
11441/154428
oai:idus.us.es:11441/154428
2024-02-01 14:59:20.434
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1378922024-02-14T11:32:55Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Pacios, Olga
Fernández-García, Laura
Bleriot, Inés
Blasco, Lucía
González-Bardanca, Mónica
Pascual Hernández, Álvaro
Tomasa, María
2022-10-13T17:55:54Z
2022-10-13T17:55:54Z
2021
Pacios, O., Fernández-García, L., Bleriot, I., Blasco, L., González-Bardanca, M., Pascual Hernández, Á. y Tomasa, M. (2021). Enhanced antibacterial activity of repurposed mitomycin C and imipenem in combination with the lytic phage vB_KpnMVAC13 against clinical isolates of klebsiella pneumoniae. Antimicrobial Agents and Chemotherapy, 65 (9), 1-11. https://doi.org/10.1128/AAC.00900-21.
0066-4804
1098-6596
https://hdl.handle.net/11441/137892
10.1128/AAC.00900-21
Klebsiella pneumoniae is an opportunistic Gram-negative pathogen that employs different strategies (resistance and persistence) to counteract antibiotic treatments. This study aimed to search for new means of combatting imipenem-resistant and persister strains of K. pneumoniae by repurposing the anticancer drug mitomycin C as an antimicrobial agent and by combining the drug and the conventional antibiotic imipenem with the lytic phage vB_KpnM-VAC13. Several clinical K. pneumoniae isolates were characterized, and an imipenem-resistant isolate (harboring OXA-245 β-lactamase) and a persister isolate were selected for study. The mitomycin C and imipenem MICs for both isolates were determined by the broth microdilution method. Time-kill curve data were obtained by optical density at 600 nm (OD600) measurement and CFU enumeration in the presence of each drug alone and with the phage. The frequency of occurrence of mutants resistant to each drug and the combinations was also calculated, and the efficacy of the combination treatments was evaluated using an in vivo infection model (Galleria mellonella). The lytic phage vB_KpnM-VAC13 and mitomycin C had synergistic effects on imipenem-resistant and persister isolates, both in vitro and in vivo. The phage-imipenem combination successfully killed the persisters but not the imipenem-resistant isolate harboring OXA-245 β-lactamase. Interestingly, the combinations decreased the emergence of in vitro resistant mutants of both isolates. Combinations of the lytic phage vB_KpnM-VAC13 with mitomycin C and imipenem were effective against the persister K. pneumoniae isolate. The lytic phage-mitomycin C combination was also effective against imipenem-resistant K. pneumoniae strains harboring OXA-245 β-lactamase
instituto de Salud Carlos III RD16/0016/0001 RD16/0016/0006 RD16/CIII/0004/0002
application/pdf
11 p.
eng
American Society Microbiology
Antimicrobial Agents and Chemotherapy, 65 (9), 1-11.
http://doi.org/10.1128/AAC.00900-21
Resistance
Persistence
Bacteriophage therapy
Drug repurposing
Synergy
Klebsiella pneumoniae
Enhanced antibacterial activity of repurposed mitomycin C and imipenem in combination with the lytic phage vB_KpnMVAC13 against clinical isolates of klebsiella pneumoniae
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/openAccess
Universidad de Sevilla. Departamento de Microbiología
Antimicrobial Agents and Chemotherapy
65
9
1
11
https://ror.org/03yxnpp24
ORIGINAL
330.pdf
330.pdf
application/pdf
1311287
https://idus.us.es/bitstream/11441/137892/1/330.pdf
a8706352c79e1c13994ec43dab9ac405
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/137892/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/137892
oai:idus.us.es:11441/137892
2024-02-14 12:32:55.562
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/869812024-02-13T22:25:24Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Newman-Griffis, Anna H.
Cerro Sánchez, Pablo del
Charpentier, Myriam
Meier, Iris
2019-05-29T14:39:22Z
2019-05-29T14:39:22Z
2019
Newman-Griffis, A.H., Cerro Sánchez, P.d., Charpentier, M. y Meier, I. (2019). Medicago LINC complexes function in nuclear morphology, nuclear movement, and root nodule symbiosis 1[OPEN]. Plant Physiology, 179 (2), 491-506.
0032-0889
1532-2548
https://hdl.handle.net/11441/86981
10.1104/pp.18.01111
Nuclear movement is involved in cellular and developmental processes across eukaryotic life, often driven by Linker of Nucleoskeleton and Cytoskeleton (LINC) complexes, which bridge the nuclear envelope (NE) via the interaction of Klarsicht/ ANC-1/Syne-1 Homology (KASH) and Sad1/UNC-84 (SUN) proteins. Arabidopsis (Arabidopsis thaliana) LINC complexes are involved in nuclear movement and positioning in several cell types. Observations since the 1950s have described targeted nuclear movement and positioning during symbiosis initiation between legumes and rhizobia, but it has not been established whether these movements are functional or incidental. Here, we identify and characterize LINC complexes in the model legume Medicago truncatula. We show that LINC complex characteristics such as NE localization, dependence of KASH proteins on SUN protein binding for NE enrichment, and direct SUN-KASH binding are conserved between plant species. Using a SUN dominant-negative strategy, we demonstrate that LINC complexes are necessary for proper nuclear shaping and movement in Medicago root hairs, and are important for infection thread initiation and nodulation.
National Science Foundation NSF-1440019, NSF-1613501
Biotechnology and Biological Sciences Research Council BB/P007112/1
European Molecular Biology Organization 6995
application/pdf
eng
American Society of Plant Biologists
Plant Physiology, 179 (2), 491-506.
NSF-1440019
NSF-1613501
BB/P007112/1
6995
http://dx.doi.org/10.1104/pp.18.01111
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Medicago LINC complexes function in nuclear morphology, nuclear movement, and root nodule symbiosis 1[OPEN]
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Plant Physiology
179
2
491
506
https://ror.org/03yxnpp24
16 p.
ORIGINAL
Medicago LINC Complexes Function.pdf
Medicago LINC Complexes Function.pdf
application/pdf
3362583
https://idus.us.es/bitstream/11441/86981/1/Medicago%20LINC%20Complexes%20Function.pdf
02514cf9d97e237d5e04b9df11ce77ae
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/86981/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/86981
oai:idus.us.es:11441/86981
2024-02-13 23:25:24.582
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1353162024-02-13T19:59:27Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Mama, Olouwafemi Mistourath
Aspiroz, Carmen
Ruiz-Ripa, Laura
Ceballos, Sara
Iñiguez Barrio, Maria
Cercenado, Emilia
Azcona, José Manuel
López Cerero, Lorena
Seral, Cristina
Torres, Carmen
2022-07-13T11:41:53Z
2022-07-13T11:41:53Z
2021
Mama, O.M., Aspiroz, C., Ruiz-Ripa, L., Ceballos, S., Iñiguez Barrio, M., Cercenado, E.,...,Torres, C. (2021). Prevalence and Genetic Characteristics of Staphylococcus aureus CC398 Isolates From Invasive Infections in Spanish Hospitals, Focusing on the Livestock-Independent CC398-MSSA Clade. Frontiers in Microbiology, 12, 623108.
1664-302X
https://hdl.handle.net/11441/135316
10.3389/fmicb.2021.623108
Background: Livestock-associated (LA)-CC398-MRSA is closely related to pigs, being unfrequently detected in human invasive infections. CC398-MSSA is emerging in human invasive infections in some countries, but genetic and epidemiological characteristics are still scarcely reported.
Objectives: To determine the prevalence of Staphylococcus aureus (SA) CC398, both MRSA and MSSA, among blood cultures SA isolates recovered in Spanish hospitals located in regions with different pig-farming densities (PD) and characterize the recovered isolates.
Methods: One thousand twenty-two SA isolates (761 MSSA, 261 MRSA) recovered from blood cultures during 6–12 months in 17 Spanish hospitals (2018–2019) were studied. CC398 lineage identification, detection of spa-types, and antibiotic resistance, virulence and human immune evasion cluster (IEC) genes were analyzed by PCR/sequencing.
Results: Forty-four CC398-MSSA isolates (4.3% of SA; 5.8% of MSSA) and 10 CC398-MRSA isolates (1% of SA; 3.8% of MRSA) were detected. Eleven spa-types were found among the CC398-MSSA isolates with t571 and t1451 the most frequent spa-types detected (75%). Most of CC398-MSSA isolates were Immune-Evasion-Cluster (IEC)-positive (88.6%), tetracycline-susceptible (95.5%) and erythromycin/clindamycin–inducible-resistant/erm(T)-positive (75%). No statistical significance was detected when the CC398-MSSA/MSSA rate was correlated to PD (pigs/km2) (p = 0.108). On the contrary, CC398-MRSA isolates were all IEC-negative, predominately spa-t011 (70%), and the CC398-MRSA/MRSA rate was significantly associated to PD (p < 0.005).
Conclusion: CC398-MSSA is an emerging clade in invasive infections in Spanish hospitals. CC398-MRSA (mostly t011) and CC398-MSSA (mostly t571 and t1451) show important differences, possibly suggesting divergent steps in host-adaptation evolutionary processes. While CC398-MRSA is livestock-associated (lacking IEC-system), CC398-MSSA seems to be mostly livestock-independent, carrying human-adaptation markers.
Agencia Estatal de Investigación (AEI) (project SAF2016-76571- R) and by the Fondo Europeo de Desarrollo Regional (FEDER)
application/pdf
10 p.
eng
Frontiers Media
Frontiers in Microbiology, 12, 623108.
SAF2016-76571-R
https://dx.doi.org/10.3389/fmicb.2021.623108
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
MSSA
LA-MRSA
CC398
Bacteremia
t571
t1451
erm(T)
Spain
Prevalence and Genetic Characteristics of Staphylococcus aureus CC398 Isolates From Invasive Infections in Spanish Hospitals, Focusing on the Livestock-Independent CC398-MSSA Clade
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Agencia Estatal de Investigación. España
European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
Frontiers in Microbiology
12
623108
https://ror.org/03yxnpp24
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/135316/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
ORIGINAL
fmicb-12-623108.pdf
fmicb-12-623108.pdf
application/pdf
494471
https://idus.us.es/bitstream/11441/135316/1/fmicb-12-623108.pdf
c3ed9a217ce1592d90b8330af837b3bf
MD5
1
open access
11441/135316
oai:idus.us.es:11441/135316
2024-02-13 20:59:27.621
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1464542024-02-14T11:38:06Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Felipe, Beatriz de
Obando Pacheco, Pablo
Carazo Gallego, Begoña
Martín López, David
Santos Pérez, José Luis
González Jiménez, Yolanda
Torres Sánchez, María José
Obando Santaella, Ignacio
2023-05-19T12:49:15Z
2023-05-19T12:49:15Z
2022-08-22
Felipe, B.d., Obando Pacheco, P., Carazo Gallego, B., Martín López, D., Santos Pérez, J.L., González Jiménez, Y.,...,Obando Santaella, I. (2022). Molecular epidemiology of paediatric invasive pneumococcal disease in Andalusia, Spain. Epidemiology & Infection, 150, PII S0950268822001376. https://doi.org/10.1017/S0950268822001376.
0950-2688; 1469-4409
https://hdl.handle.net/11441/146454
10.1017/S0950268822001376
This study aimed to assess the impact of the introduction of pneumococcal conjugate vaccine 13 (PCV13) on the molecular epidemiology of invasive pneumococcal disease (IPD) in children from Andalusia. A population-based prospective surveillance study was conducted on IPD in children aged <14 years from Andalusia (2018–2020). Pneumococcal invasive isolates collected between 2006 and 2009 in the two largest tertiary hospitals in Andalusia were used as pre-PCV13 controls for comparison of serotype/genotype distribution. Overall IPD incidence rate was 3.55 cases per 100 000 in 2018; increased non-significantly to 4.20 cases per 100 000 in 2019 and declined in 2020 to 1.69 cases per 100 000 (incidence rate ratio 2020 vs. 2019: 0.40, 95% confidence interval (CI) 0.20–0.89, P = 0.01). Proportion of IPD cases due to PCV13 serotypes in 2018–2020 was 28% (P = 0.0001 for comparison with 2006–2009). Serotypes 24F (15%) and 11A (8.3%) were the most frequently identified non-PCV13 serotypes (NVT) in 2018–2020. Penicillin- and/or ampicillin-resistant clones mostly belonged to clonal complex 156 (serotype 14-ST156 and ST2944 and serotype 11A-ST6521). The proportion of IPD cases caused by PCV13 serotypes declined significantly after the initiation of the PCV13 vaccination programme in 2016. Certain NVT, such as serotypes 24F and 11A, warrant future monitoring in IPD owing to invasive potential and/or antibiotic resistance rates.
Pfizer
application/pdf
8 p.
eng
Cambridge University Press
Epidemiology & Infection, 150, PII S0950268822001376.
53233485
https://www.cambridge.org/core/journals/epidemiology-and-infection/article/molecular-epidemiology-of-paediatric-invasive-pneumococcal-disease-in-andalusia-spain/AFEB3E9440E11D50F47F73E24E0353CC
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Molecular epidemiology of paediatric invasive pneumococcal disease in Andalusia, Spain
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Epidemiology & Infection
150
PII S0950268822001376
https://ror.org/03yxnpp24
ORIGINAL
Molecular epidemiology of paediatric invasive pneumococcal disease in Andalusia, Spain.pdf
Molecular epidemiology of paediatric invasive pneumococcal disease in Andalusia, Spain.pdf
application/pdf
347784
https://idus.us.es/bitstream/11441/146454/1/Molecular%20epidemiology%20of%20paediatric%20invasive%20pneumococcal%20disease%20in%20Andalusia%2c%20Spain.pdf
52c60121cf0ba999aeb306265ddc4ba8
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1138
https://idus.us.es/bitstream/11441/146454/2/license.txt
d037f995682d8bf558422b812ec0f36c
MD5
2
open access
11441/146454
oai:idus.us.es:11441/146454
2024-02-14 12:38:06.781
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/366882024-02-17T16:50:18Zcom_11441_10878com_11441_10802com_11441_10690com_11441_10903col_11441_10879col_11441_10904
Ramos Morales, Francisco
Infante, Carlos R.
Fedriani Iriso, Concepción
Bornens, Michel
Ríos Sánchez, Rosa María
2016-03-01T13:03:02Z
2016-03-01T13:03:02Z
1999
Ramos Morales, F., Infante, C.R., Fedriani Iriso, C., Bornens, M. y Ríos Sánchez, R.M. (1999). Gmap-210, a cis-Golgi network-associated protein, is a minus end microtubule-binding protein.
0021-9525
http://hdl.handle.net/11441/36688
10.1083/jcb.145.1.83
https://idus.us.es/xmlui/handle/11441/36688
We report that a peripheral Golgi protein
with a molecular mass of 210 kD localized at the cis-
Golgi network (Rios, R.M., A.M. Tassin, C. Celati, C.
Antony, M.C. Boissier, J.C. Homberg, and M. Bornens.
1994.
J. Cell Biol.
125:997Ð1013) is a microtubule-binding
protein that associates in situ with a subpopulation
of stable microtubules. Interaction of this protein, now
called GMAP-210, for Golgi microtubule-associated
protein 210, with microtubules in vitro is direct, tight
and nucleotide-independent. Biochemical analysis further
suggests that GMAP-210 specifically binds to
microtubule ends. The full-length cDNA encoding
GMAP-210 predicts a protein of 1,979 amino acids with
a very long central coiled-coil domain. Deletion analyses
in vitro show that the COOH terminus of GMAP-
210 binds to microtubules whereas the NH
2
terminus
binds to Golgi membranes. Overexpression of GMAP-
210Ðencoding cDNA induced a dramatic enlargement
of the Golgi apparatus and perturbations in the microtubule
network. These effects did not occur when a mutant
lacking the COOH-terminal domain was expressed.
When transfected in fusion with the green
fluorescent protein, the NH
2
-terminal domain associated
with the cis-Golgi network whereas the COOHterminal
microtubule-binding domain localized at the
centrosome. Altogether these data support the view
that GMAP-210 serves to link the cis-Golgi network to
the minus ends of centrosome-nucleated microtubules.
In addition, this interaction appears essential for ensuring
the proper morphology and size of the Golgi apparatus
application/pdf
eng
Rockefeller University Press
null
http://jcb.rupress.org/content/145/1/83.full.pdf+html
http://dx.doi.org/10.1083/jcb.145.1.83
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
autoantigens
Golgi apparatus
microtubule- associated proteins
microtubules
centrosome
Gmap-210, a cis-Golgi network-associated protein, is a minus end microtubule-binding protein
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Genética
Universidad de Sevilla. Departamento de Microbiología
https://ror.org/03yxnpp24
ORIGINAL
GMAP-210, a cis.pdf
GMAP-210, a cis.pdf
application/pdf
697291
https://idus.us.es/bitstream/11441/36688/1/GMAP-210%2c%20a%20cis.pdf
35ee0e570cbb9173322e758102439e86
MD5
1
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CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/36688/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/36688/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/36688
oai:idus.us.es:11441/36688
2024-02-17 17:50:18.113
open access
idUS - Universidad de Sevilla
idus@us.es
RGVjbGFyYSBxdWU6CgphKSBFcyBhdXRvciB5IHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIHByb3BpZWRhZCBpbnRlbGVjdHVhbC4KCgpiKSBTaSBleGlzdGUgcHJldmlhIGNlc2nDs24gYSB0ZXJjZXJvcyBkZSBsb3MgZGVyZWNob3MgZGUgZXhwbG90YWNpw7NuIGRlIGxhIG9icmEsIGN1ZW50YSBjb24gbGEgYXV0b3JpemFjacOzbiBkZSBkaWNob3MgdGl0dWxhcmVzLiAKCgpjKSBTaSBlbCBkb2N1bWVudG8gY29udGllbmUgbWF0ZXJpYWxlcyBkZSBsb3MgcXVlIG5vIGVzIHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIGV4cGxvdGFjacOzbiwgIHRpZW5lIGVsIHBlcm1pc28gcGFyYSBkZXBvc2l0YXJsb3MgeSBxdWUgZXNlIG1hdGVyaWFsIGVzdMOhIGlkZW50aWZpY2FkbyBjbGFyYW1lbnRlLgoKCkVsIGF1dG9yIHJlYWxpemEgbGEgY2VzacOzbiBncmF0dWl0YSB5IG5vIGV4Y2x1c2l2YSBhIGxhIFVuaXZlcnNpZGFkIGRlIFNldmlsbGEgZGUgbG9zIGRlcmVjaG9zIGRlIHJlcHJvZHVjY2nDs24sIGRpc3RyaWJ1Y2nDs24sIGNvbXVuaWNhY2nDs24gcMO6YmxpY2EgeSB0cmFuc2Zvcm1hY2nDs247IHBlcm1pdGllbmRvIGFsIHJlcG9zaXRvcmlvOgoKCglUcmFuc2Zvcm1hY2nDs24gZGVsIGZvcm1hdG8gcGFyYSBzdSBpbmNvcnBvcmFjacOzbiB5IHByZXNlcnZhY2nDs24uCgoKCVJlcHJvZHVjY2nDs24geSBhcmNoaXZvICBlbiBsb3Mgc2Vydmlkb3JlcyBhc29jaWFkb3MgYWwgcmVwb3NpdG9yaW8uCgoKCVN1IGNvbXVuaWNhY2nDs24gcMO6YmxpY2EgeSBzdSBwdWVzdGEgYSBkaXNwb3NpY2nDs24gZGUgbW9kbyBsaWJyZSB5IGdyYXR1aXRvIGEgdHJhdsOpcyBkZWwgYXJjaGl2byBhYmllcnRvIGluc3RpdHVjaW9uYWwKCgpFbCBhdXRvciBhdXRvcml6YSBxdWUgbGEgb2JyYSBzZSBwb25nYSBhIGRpc3Bvc2ljacOzbiBkZSBsb3MgdXN1YXJpb3MgYSB0cmF2w6lzIGRlbCByZXBvc2l0b3JpbyBpbnN0aXR1Y2lvbmFsIHBhcmEgcXVlIHNlIGhhZ2EgdW4gdXNvIGp1c3RvLCByZXNwZXRhbmRvIGxvcyBkZXJlY2hvcyBkZSBhdXRvciBxdWUgbGEgbGVnaXNsYWNpw7NuIGVzdGFibGVjZSB5IGNvbmZvcm1lIGNvbiBsYXMgY29uZGljaW9uZXMgbWFyY2FkYXMgcG9yIGxhIGxpY2VuY2lhIGRlIHVzbwo=
oai:idus.us.es:11441/965632024-02-14T09:07:55Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Marco, Diana E.
Carbajal, Juan P.
Cannas, Sergio
Pérez Arnedo, Rebeca
Hidalgo Perea, Ángeles
Olivares, José
Ruiz Sainz, José Enrique
Sanjuán, Juan
2020-05-13T14:10:50Z
2020-05-13T14:10:50Z
2009
Marco, D.E., Carbajal, .P., Cannas, S., Pérez Arnedo, R., Hidalgo Perea, Á., Olivares, J.,...,Sanjuán, J. (2009). An experimental and modelling exploration of the host-sanction hypothesis in legume-rhizobia mutualism. Journal of Theoretical Biology, 259 (3), 423-433.
1095-8541
https://hdl.handle.net/11441/96563
10.1016/j.jtbi.2009.03.033
Despite the importance of mutualism as a key ecological process, its persistence in
nature is difficult to explain since the existence of exploitative, 'cheating' partners that
could erode the interaction is common. By analogy with the proposed policing strategy
Nature Precedings : hdl:10101/npre.2008.1964.1 : Posted 10 Jun 2008
2
stabilizing intraspecific cooperation, host sanctions against non N2 fixing, cheating
symbionts have been proposed as a force stabilizing mutualism in legume-Rhizobium
symbiosis. Following this proposal, penalizations would include decreased nodular
rhizobial viability and/or early nodule senescence in nodules occupied by cheating
rhizobia. In this work, we analyze the stability of Rhizobium-legume symbiosis when
"cheating" strains are present, using an experimental and modelling approach. We used
split-root experiments with soybean plants inoculated with two rhizobial strains, a
cooperative, normal N2 fixing strain and an isogenic non-fixing, “perfect” cheating
mutant derivative that lacks nitrogenase activity but has the same nodulation abilities
inoculated to split-root plants. We found no experimental evidence of functioning plant
host sanctions to cheater rhizobia based on nodular rhizobia viability and nodule
senescence and maturity molecular markers. Based on these experiments, we developed
a population dynamic model with and without the inclusion of plant host sanctions. We
show that plant populations persist in spite of the presence of cheating rhizobia without
the need of incorporating any sanction against the cheater populations in the model,
under the realistic assumption that plants can at least get some amount of fixed N2 from
the effectively mutualistic rhizobia occupying some nodules. Inclusion of plant
sanctions merely reduces the time needed for reaching plant population equilibrium and
leads to the unrealistic effect of ultimate extinction of cheater strains in soil. Our
simulation results are in agreement with increasing experimental evidence and
theoretical work showing that mutualisms can persist or even improve in presence of
cheating partners.
application/pdf
10 p.
eng
Elsevier
Journal of Theoretical Biology, 259 (3), 423-433.
https://doi.org/10.1016/j.jtbi.2009.03.033
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
mutualism
cheating
legume-rhizobia symbiosis
host sanctions
experimentally-based modelling
An experimental and modelling exploration of the host-sanction hypothesis in legume-rhizobia mutualism
info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
Universidad de Sevilla. Departamento de Microbiología
Journal of Theoretical Biology
259
3
423
433
https://ror.org/03yxnpp24
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/96563/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
ORIGINAL
pubpub.pdf
pubpub.pdf
application/pdf
992099
https://idus.us.es/bitstream/11441/96563/1/pubpub.pdf
5e09a4d377a97086b1d48b917cb0b177
MD5
1
open access
11441/96563
oai:idus.us.es:11441/96563
2024-02-14 10:07:55.441
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1355692024-02-14T19:16:00Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Giráldez Macías, Servando
Herrero Ruiz, Joaquín
Mora Santos, María del Mar
Japón, Miguel Ángel
Tortolero García, María Dolores
Romero Portillo, Francisco
2022-07-19T11:04:10Z
2022-07-19T11:04:10Z
2014
Giráldez Macías, S., Herrero Ruiz, J., Mora Santos, M.d.M., Japón, M.Á., Tortolero García, M.D. y Romero Portillo, F. (2014). SCFFBXW7α modulates the intra-S-phase DNA-damage checkpoint by regulating Polo like kinase-1 stability. Oncotarget, 5 (12), 4370-4383.
1949-2553
https://hdl.handle.net/11441/135569
10.18632/oncotarget.2021
The intra-S-checkpoint is essential to control cell progression through S phase under normal conditions and in response to replication stress. When DNA lesions are detected, replication fork progression is blocked allowing time for repair to avoid genomic instability and the risk of cancer. DNA replication initiates at many origins of replication in eukaryotic cells, where a series of proteins form pre-replicative complexes (pre-RCs) that are activated to become pre-initiation complexes and ensure a single round of replication in each cell cycle. PLK1 plays an important role in the regulation of DNA replication, contributing to the regulation of pre-RCs formation by phosphorylating several proteins, under both normal and stress conditions. Here we report that PLK1 is ubiquitinated and degraded by SCFFBXW7α/proteasome. Moreover, we identified a new Cdc4 phosphodegron in PLK1, conserved from yeast to humans, whose mutation prevents PLK1 destruction. We established that endogenous SCFFBXW7α degrades PLK1 in the G1 and S phases of an unperturbed cell cycle and in S phase following UV irradiation. Furthermore, we showed that FBXW7α overexpression or UV irradiation prevented the loading of proteins onto chromatin to form pre-RCs and, accordingly, reduced cell proliferation. We conclude that PLK1 degradation mediated by SCFFBXW7α modulates the intra-S-phase checkpoint.
Ministerio de Economía y Competitividad, MINECO SAF2011-30003
Junta de Andalucía P08- CVI-03603
application/pdf
14 p.
eng
Impact Journals LLC
Oncotarget, 5 (12), 4370-4383.
SAF2011-30003
P08-CVI-03603
10.18632/oncotarget.2021
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
PLK1
FBXW7
intra-S-phase
DNA-damage
Proteasome
Protein degradation
SCFFBXW7α modulates the intra-S-phase DNA-damage checkpoint by regulating Polo like kinase-1 stability
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Ministerio de Economía y Competitividad (MINECO). España
Junta de Andalucía
Oncotarget
5
12
4370
4383
https://ror.org/03yxnpp24
ORIGINAL
oncotarget-v5i12-2021.pdf
oncotarget-v5i12-2021.pdf
application/pdf
2472493
https://idus.us.es/bitstream/11441/135569/1/oncotarget-v5i12-2021.pdf
b9fc73c612a56159af8ee3cd12d01b2b
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/135569/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/135569
oai:idus.us.es:11441/135569
2024-02-14 20:16:00.126
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1394092024-02-13T20:10:36Zcom_11441_11044com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11045col_11441_10904
Salto-Alejandre, Sonsoles
Berastegui-Cabrera, Judith
Camacho-Martínez, Pedro
Infante-Domínguez, Carmen
Carretero-Ledesma, Marta
Crespo-Rivas, Juan Carlos
Lepe Jiménez, José Antonio
Cisneros, José Miguel
Pachón Díaz, Jerónimo
Cordero Matia, María Elisa
Sánchez-Céspedes, Javier
Barón-Franco, Bosco
Bernabeu Wittel, Máximo
López Cortés, Luis Fernando
Nieto Martín, María Dolores
Romero Rodríguez, María de las Nieves
Ampuero Herrojo, Javier
2022-11-14T16:12:44Z
2022-11-14T16:12:44Z
2021
Salto-Alejandre, S., Berastegui-Cabrera, J., Camacho-Martínez, P., Infante-Domínguez, C., Carretero-Ledesma, M., Crespo-Rivas, J.C.,...,Ampuero Herrojo, J. (2021). SARS-CoV-2 viral load in nasopharyngeal swabs is not an independent predictor of unfavorable outcome. Scientific Reports, 11 (1), 12931. https://doi.org/10.1038/s41598-021-92400-y.
2045-2322
https://hdl.handle.net/11441/139409
10.1038/s41598-021-92400-y
The aim was to assess the ability of nasopharyngeal SARS-CoV-2 viral load at frst patient’s hospital
evaluation to predict unfavorable outcomes. We conducted a prospective cohort study including 321
adult patients with confrmed COVID-19 through RT-PCR in nasopharyngeal swabs. Quantitative
Synthetic SARS-CoV-2 RNA cycle threshold values were used to calculate the viral load in log10
copies/mL. Disease severity at the end of follow up was categorized into mild, moderate, and severe.
Primary endpoint was a composite of intensive care unit (ICU) admission and/or death (n= 85,
26.4%). Univariable and multivariable logistic regression analyses were performed. Nasopharyngeal
SARS-CoV-2 viral load over the second quartile (≥7.35 log10 copies/mL, p = 0.003) and second tertile
(≥ 8.27 log10 copies/mL, p = 0.01) were associated to unfavorable outcome in the unadjusted logistic
regression analysis. However, in the fnal multivariable analysis, viral load was not independently
associated with an unfavorable outcome. Five predictors were independently associated with
increased odds of ICU admission and/or death: age≥ 70 years, SpO2, neutrophils > 7.5 × 103
/µL,
lactate dehydrogenase≥ 300 U/L, and C-reactive protein≥ 100 mg/L. In summary, nasopharyngeal
SARS-CoV-2 viral load on admission is generally high in patients with COVID-19, regardless of illness
severity, but it cannot be used as an independent predictor of unfavorable clinical outcome.
application/pdf
9 p.
eng
NATURE PUBLISHING GROUP
Scientific Reports, 11 (1), 12931.
REIPI RD16/0016/0009
COV20/00370
COV20/00580
C-0059-2018
https://www.nature.com/articles/s41598-021-92400-y
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
SARS-CoV-2 viral load in nasopharyngeal swabs is not an independent predictor of unfavorable outcome
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Medicina
National Plan R+D+I 2013–2016
Instituto de Salud Carlos III
Ministerio de Economía, Industria y Competitividad. España
Servicio Andaluz de Salud, Junta de Andalucía, España.
Scientific Reports
11
1
12931
https://ror.org/03yxnpp24
ORIGINAL
SARS‑CoV‑2...pdf
SARS‑CoV‑2...pdf
application/pdf
1066795
https://idus.us.es/bitstream/11441/139409/1/SARS%e2%80%91CoV%e2%80%912...pdf
b8b09016eb16b6778c59bb73e0898053
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/139409/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/139409
oai:idus.us.es:11441/139409
2024-02-13 21:10:36.393
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1562772024-03-14T15:31:21Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Kretschmer, Marcel
Müller, Julia
Henke, Petra
Otto, Viktoria
Arce Rodríguez, Alejandro
Müsken, Mathias
Jahn, Dieter
Borrero de Acuña, José Manuel
Neumann-Schaal, Meina
Wegner, Andre
2024-03-14T15:31:21Z
2024-03-14T15:31:21Z
2023-11-21
Kretschmer, M., Müller, J., Henke, P., Otto, V., Arce Rodríguez, A., Müsken, M.,...,Wegner, A. (2023). Isolation and Quantification of Bacterial Membrane Vesicles for Quantitative Metabolic Studies Using Mammalian Cell Cultures. Cells, 12 (23), 2674. https://doi.org/10.3390/cells12232674.
2073-4409
https://hdl.handle.net/11441/156277
10.3390/cells12232674
Bacterial membrane vesicles (BMVs) are produced by most bacteria and participate in various cellular processes, such as intercellular communication, nutrient exchange, and pathogenesis. Notably, these vesicles can contain virulence factors, including toxic proteins, DNA, and RNA. Such factors can contribute to the harmful effects of bacterial pathogens on host cells and tissues. Although the general effects of BMVs on host cellular physiology are well known, the underlying molecular mechanisms are less understood. In this study, we introduce a vesicle quantification method, leveraging the membrane dye FM4-64. We utilize a linear regression model to analyze the fluorescence emitted by stained vesicle membranes to ensure consistent and reproducible vesicle–host interaction studies using cultured cells. This method is particularly valuable for identifying host cellular processes impacted by vesicles and their specific cargo. Moreover, it outcompetes unreliable protein concentration-based methods. We (1) show a linear correlation between the number of vesicles and the fluorescence signal emitted from the FM4-64 dye; (2) introduce the “vesicle load” as a new semi-quantitative unit, facilitating more reproducible vesicle-cell culture interaction experiments; (3) show that a stable vesicle load yields consistent host responses when studying vesicles from Pseudomonas aeruginosa mutants; (4) demonstrate that typical vesicle isolation contaminants, such as flagella, do not significantly skew the metabolic response of lung epithelial cells to P. aeruginosa vesicles; and (5) identify inositol monophosphatase 1 (SuhB) as a pivotal regulator in the vesicle-mediated pathogenesis of P. aeruginosa.
application/pdf
13 p.
eng
Multidisciplinary Digital Publishing Institute (MDPI)
Cells, 12 (23), 2674.
https://doi.org/10.3390/cells12232674
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Bacterial membrane vesicles (BMVs)
Membrane vesicles (MVs)
Metabolism
Outer membrane vesicles (OMVs)
Pathogen
Pseudomonas aeruginosa
Quantification
SuhB
Vesicle isolation
Isolation and Quantification of Bacterial Membrane Vesicles for Quantitative Metabolic Studies Using Mammalian Cell Cultures
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Cells
12
23
2674
ORIGINAL
Isolation and Quantification.pdf
Isolation and Quantification.pdf
Versión publicada
application/pdf
10280013
https://idus.us.es/bitstream/11441/156277/1/Isolation%20and%20Quantification.pdf
236ad9961102c26de36434846c93d898
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1142
https://idus.us.es/bitstream/11441/156277/2/license.txt
4ccdb332745e121f04fdde82917fec0c
MD5
2
open access
11441/156277
oai:idus.us.es:11441/156277
2024-03-14 16:31:21.222
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1544182024-02-01T14:46:32Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Machuca, Jesús
López Cerero, Lorena
Fernández-Cuenca, Felipe
Mora-Navas, Laura
Mediavilla-Gradolph, Concepción
López-Rodríguez, Inmaculada
Pascual Hernández, Álvaro
2024-02-01T12:40:11Z
2024-02-01T12:40:11Z
2019
0066-4804
1098-6596
https://hdl.handle.net/11441/154418
10.1128/AAC.01396-18
The aim of this study was to characterize the population structure of 56 OXA-48-like-producing Klebsiella pneumoniae isolates, as well as extended-spectrum β-lactamase (ESBL) and carbapenemase genes, recovered in 2014 and 2015 from 16 hospitals in southern Spain. XbaI pulsed-field gel electrophoresis and multilocus sequence typing were performed to assess clonal relatedness. Representative isolates belonging to OXA-48-like-producing and CTX-M-15-coproducing pulsotypes were selected for characterization of blaOXA-48-like- and blaCTX-M-15-carrying plasmids by PCR-based replicon typing, IncF subtyping, whole-genome sequencing analysis, and typing of Tn1999 structures. Forty-three OXA-48-producing isolates (77%) were recovered from clinical samples and 13 from rectal swabs. All isolates showed ertapenem MIC values of ≥1 mg/liter, although 70% remained susceptible to imipenem and meropenem. Forty-nine isolates (88%) produced OXA-48, 5 produced OXA-245, and 2 produced OXA-181. Twenty-eight different pulsotypes (5 detected in more than 1 hospital) and 16 sequence types (STs) were found. The most prevalent clones were ST15 (29 isolates [52%]) and ST11 (7 isolates [13%]). Forty-five (80%) isolates were also blaCTX-M-15 carriers. The blaCTX-M-15 gene was mostly (82%) located on IncR plasmids, although ST15 and ST11 isolates also carried this gene on IncF plasmids. The composite transposon variant Tn1999.2-like was the most frequent. Among ST15 and ST11 isolates, different transposon variants were observed. The blaOXA-48 gene was mainly located on IncL plasmids, although IncM plasmids were also observed. The spread of OXA-48-like-producing K. pneumoniae in southern Spain is mainly due to ST15 and ST11 clones. Variation within clonal lineages could indicate different acquisition events for both ESBL and carbapenemase traits.
application/pdf
8 p.
eng
Oxford University Press
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Klebsiella pneumoniae
OXA-48
Southern Spain
OXA-48-Like-Producing Klebsiella pneumoniae in Southern Spain in 2014-2015
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Microbiología
European Development Regional Fund
Instituto de Salud Carlos III, Subdireccion General de Redes y Centros de Investigacion Cooperativa, Ministerio de Ciencia, Innovacion y Universidades
Plan Nacional de I + D + i 2013-2016
Spanish Network for Research in Infectious Diseases
REIPI RD16/0016/0001
https://journals.asm.org/doi/10.1128/aac.01396-18
Antimicrobial Agents and Chemotherapy
63
1
e01396-18
ORIGINAL
OXA-48-Like-Producing Klebsiella pneumoniae in Southern Spain in 2014-2015.pdf
OXA-48-Like-Producing Klebsiella pneumoniae in Southern Spain in 2014-2015.pdf
application/pdf
406912
https://idus.us.es/bitstream/11441/154418/1/OXA-48-Like-Producing%20Klebsiella%20pneumoniae%20in%20Southern%20Spain%20in%202014-2015.pdf
f4ec1dc9704d447484bdb56f8d9ec63d
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1142
https://idus.us.es/bitstream/11441/154418/2/license.txt
4ccdb332745e121f04fdde82917fec0c
MD5
2
open access
11441/154418
oai:idus.us.es:11441/154418
2024-02-01 15:46:32.201
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/294162024-02-14T13:32:35Zcom_11441_10903com_11441_10802com_11441_10690com_11441_10923col_11441_10904col_11441_10924
Rodríguez Navarro, Dulce Nombre
Rodríguez Carvajal, Miguel Ángel
Acosta Jurado, Sebastián
Soto Misffut, María José
Margaret Oliver, Isabel María
Crespo Rivas, Juan Carlos
Sanjuan, Juan
Temprano Vera, Francisco Jesús
Gil Serrano, Antonio Miguel
Ruiz Sainz, José Enrique
Vinardell González, José María
2015-10-14T08:54:36Z
2015-10-14T08:54:36Z
2014
1932-6203
1932-6203
http://hdl.handle.net/11441/29416
http://dx.doi.org/10.1371/journal.pone.0115391
https://idus.us.es/xmlui/handle/11441/29416
Here we report that the structure of the Sinorhizobium fredii HH103 exopolysaccharide (EPS) is composed of glucose, galactose, glucuronic acid, pyruvic acid, in the ratios 5:2:2:1 and is partially acetylated. A S. fredii HH103 exoA mutant (SVQ530), unable to produce EPS, not only forms nitrogen fixing nodules with soybean but also shows increased competitive capacity for nodule occupancy. Mutant SVQ530 is, however, less competitive to nodulate Vigna unguiculata. Biofilm formation was reduced in mutant SVQ530 but increased in an EPS overproducing mutant. Mutant SVQ530 was impaired in surface motility and showed higher osmosensitivity compared to its wild type strain in media containing 50 mM NaCl or 5% (w/v) sucrose. Neither S. fredii HH103 nor 41 other S. fredii strains were recognized by soybean lectin (SBL). S. fredii HH103 mutants affected in exopolysaccharides (EPS), lipopolysaccharides (LPS), cyclic glucans (CG) or capsular polysaccharides (KPS) were not significantly impaired in their soybean-root attachment capacity, suggesting that these surface polysaccharides might not be relevant in early attachment to soybean roots. These results also indicate that the molecular mechanisms involved in S. fredii attachment to soybean roots might be different to those operating in Bradyrhizobium japonicum.
application/pdf
eng
Public Library of Science
PLoS One, 9(12)
http://dx.doi.org/10.1371/journal.pone.0115391
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Soybean
Polysaccharides
Symbiosis
Bacterial biofilms
Lectins
Pathogen motility
Bradyrhizobium
Galactose
Structure and biological roles of Sinorhizobium fredii HH103 exopolysaccharide
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Química orgánica
Universidad de Sevilla. Departamento de Microbiología
https://ror.org/03yxnpp24
ORIGINAL
journal.pone.0115391.pdf
journal.pone.0115391.pdf
application/pdf
1407957
https://idus.us.es/bitstream/11441/29416/1/journal.pone.0115391.pdf
ba085d011158a7e5c5d2921499457b31
MD5
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open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/29416/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/29416/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/29416
oai:idus.us.es:11441/29416
2024-02-14 14:32:35.362
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1299872024-02-13T09:23:21Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Furniss, R Christopher D.
Kaderabkova, Nikol
Barker, Declan
Bernal Guzmán, Patricia
Maslova, Evgenia
Antwi, Amanda Aa
McNeil, Helen E
Pugh, Hannah L.
Dortet, Laurent
Mavridou, Despoina Ai
2022-02-15T14:54:22Z
2022-02-15T14:54:22Z
2022
Furniss, R.C.D., Kaderabkova, N., Barker, D., Bernal Guzmán, P., Maslova, E., Antwi, A.A.,...,Mavridou, D.A. (2022). Breaking antimicrobial resistance by disrupting extracytoplasmic protein folding. eLife, 11, e57974.
2050-084X
https://hdl.handle.net/11441/129987
10.7554/eLife.57974
Antimicrobial resistance in Gram-negative bacteria is one of the greatest threats to global health. New antibacterial strategies are urgently needed, and the development of antibiotic adjuvants that either neutralize resistance proteins or compromise the integrity of the cell envelope is of ever-growing interest. Most available adjuvants are only effective against specific resistance proteins. Here we demonstrate that disruption of cell envelope protein homeostasis simultaneously compromises several classes of resistance determinants. In particular, we find that impairing DsbA-mediated disulfide bond formation incapacitates diverse β-lactamases and destabilizes mobile colistin resistance enzymes. Furthermore, we show that chemical inhibition of DsbA sensitizes multidrug-resistant clinical isolates to existing antibiotics and that the absence of DsbA, in combination with antibiotic treatment, substantially increases the survival of Galleria mellonella larvae infected with multidrug-resistant Pseudomonas aeruginosa. This work lays the foundation for the development of novel antibiotic adjuvants that function as broad-acting resistance breakers.
British Society for Antimicrobial Chemotherapy BSAC-2018-0095
NC3Rs NC/V001582/1
Biological Sciences Research Council BB/V007823/1
Academy of Medical Sciences SBF006\1040
application/pdf
69 p.
eng
eLife Sciences Publications
eLife, 11, e57974.
BSAC-2018-0095
NC/V001582/1
BB/V007823/1
SBF006\1040
https://doi.org/10.7554/eLife.57974
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Breaking antimicrobial resistance by disrupting extracytoplasmic protein folding
info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
Universidad de Sevilla. Departamento de Microbiología
eLife
11
e57974
https://ror.org/03yxnpp24
ORIGINAL
Breaking antimicrobial resistance.pdf
Breaking antimicrobial resistance.pdf
application/pdf
39686919
https://idus.us.es/bitstream/11441/129987/1/Breaking%20antimicrobial%20resistance.pdf
c34b2573492cf1cd220c472466eb1097
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/129987/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/129987
oai:idus.us.es:11441/129987
2024-02-13 10:23:21.287
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/624862024-02-14T13:54:26Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Villalobo Polo, Eduardo
Moch, Clara
Fryd Versavel, Ghislaine
Fleury Aubusson, Anne
Morin, Loïc
2017-07-13T14:33:41Z
2017-07-13T14:33:41Z
2003-12
Villalobo Polo, E., Moch, C., Fryd Versavel, G., Fleury Aubusson, A. y Morin, L. (2003). Cysteine Proteases and Cell Differentiation: Excystment of the Ciliated Protist Sterkiella histriomuscorum. Eukaryotic Cell, 2 (6), 1234-1245.
1535-9778 (impreso)
1535-9786 (electronico)
http://hdl.handle.net/11441/62486
10.1128/EC.2.6.1234–1245.2003
The process of excystment of Sterkiella histriomuscorum (Ciliophora, Oxytrichidae) leads in a few hours, through a massive influx of water and the resorption of the cyst wall, from an undifferentiated resting cyst to a highly differentiated and dividing vegetative cell. While studying the nature of the genes involved in this process, we isolated three different cysteine proteases genes, namely, a cathepsin B gene, a cathepsin L-like gene, and a calpain-like gene. Excystation was selectively inhibited at a precise differentiating stage by cysteine proteases inhibitors, suggesting that these proteins are specifically required during the excystment process. Reverse transcription-PCR experiments showed that both genes display differential expression between the cyst and the vegetative cells. A phylogenetic analysis showed for the first time that the cathepsin B tree is paraphyletic and that the diverging S. histriomuscorum cathepsin B is closely related to its Giardia homologues, which take part in the cyst wall breakdown process. The deduced cathepsin L-like protein sequence displays the structural signatures and phylogenetic relationships of cathepsin H, a protein that is known only in plants and animals and that is involved in the degradation of extracellular matrix components in cancer diseases. The deduced calpain-like protein sequence does not display the calcium-binding domain of conventional calpains; it belongs to a diverging phylogenetic cluster that includes Aspergillus palB, a protein which is involved in a signal transduction pathway that is sensitive to ambient pH.
application/pdf
eng
American Society for Microbiology
Eukaryotic Cell, 2 (6), 1234-1245.
http://dx.doi.org/10.1128/EC.2.6.1234–1245.2003
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Cysteine Proteases and Cell Differentiation: Excystment of the Ciliated Protist Sterkiella histriomuscorum
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Eukaryotic Cell
2
6
1234
1245
https://ror.org/03yxnpp24
12 p.
ORIGINAL
Cysteine Proteases and Cell Differentiation.pdf
Cysteine Proteases and Cell Differentiation.pdf
application/pdf
1736028
https://idus.us.es/bitstream/11441/62486/1/Cysteine%20Proteases%20and%20Cell%20Differentiation.pdf
82149ec60563077bcb2c4f86386479e0
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/62486/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/62486/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/62486
oai:idus.us.es:11441/62486
2024-02-14 14:54:26.075
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/688072024-02-17T17:22:08Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Rachwał, Kamila
Lipa, Paulina
Wojda, Iwona
Vinardell González, José María
Janczarek, Monika
2018-01-11T15:19:25Z
2018-01-11T15:19:25Z
2017
Rachwał, K., Lipa, P., Wojda, I., Vinardell González, J.M. y Janczarek, M. (2017). Regulatory elements located in the upstream region of the Rhizobium leguminosarum rosR global regulator are essential for its transcription and mRNA stability. Genes, 8 (12), 388-.
2073-4425
http://hdl.handle.net/11441/68807
10.3390/genes8120388
Rhizobium leguminosarum bv. trifolii is a soil bacterium capable of establishing a symbiotic relationship with clover (Trifolium spp.). Previously, the rosR gene, encoding a global regulatory protein involved in motility, synthesis of cell-surface components, and other cellular processes was identified and characterized in this bacterium. This gene possesses a long upstream region that contains several regulatory motifs, including inverted repeats (IRs) of different lengths. So far, the role of these motifs in the regulation of rosR transcription has not been elucidated in detail. In this study, we performed a functional analysis of these motifs using a set of transcriptional rosR-lacZ fusions that contain mutations in these regions. The levels of rosR transcription for different mutant variants were evaluated in R. leguminosarum using both quantitative real-time PCR and β-galactosidase activity assays. Moreover, the stability of wild type rosR transcripts and those with mutations in the regulatory motifs was determined using an RNA decay assay and plasmids with mutations in different IRs located in the 5′ -untranslated region of the gene. The results show that transcription of rosR undergoes complex regulation, in which several regulatory elements located in the upstream region and some regulatory proteins are engaged. These include an upstream regulatory element, an extension of the -10 element containing three nucleotides TGn (TGn-extended -10 element), several IRs, and PraR repressor related to quorum sensing.
Polish National Science Centre DEC-2012/07/B/NZ1/00099
application/pdf
eng
Multidisciplinary Digital Publishing Institute (MDPI)
Genes, 8 (12), 388-.
DEC-2012/07/B/NZ1/00099
http://dx.doi.org/10.3390/genes8120388
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Gene expression
Rhizobium leguminosarum
RNA secondary structures
RNA stability
rosR
Symbiotic bacteria
Transcription regulation
Regulatory elements located in the upstream region of the Rhizobium leguminosarum rosR global regulator are essential for its transcription and mRNA stability
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
National Science Center. Poland
Genes
8
12
388
https://ror.org/03yxnpp24
21 p.
ORIGINAL
Regulatory Elements Located.pdf
Regulatory Elements Located.pdf
application/pdf
3391797
https://idus.us.es/bitstream/11441/68807/1/Regulatory%20Elements%20Located.pdf
5490a06cf7f60272b90bcf80b7ed655e
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/68807/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/68807/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/68807
oai:idus.us.es:11441/68807
2024-02-17 18:22:08.141
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1390792024-02-13T22:12:59Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Ayala García, Paula
Jiménez Guerrero, Irene
Jacott, Catherine N.
López Baena, Francisco Javier
Ollero Márquez, Francisco Javier
Cerro Sánchez, Pablo del
Pérez Montaño, Francisco de Asís
2022-11-07T12:01:39Z
2022-11-07T12:01:39Z
2022
Ayala García, P., Jiménez Guerrero, I., Jacott, C.N., López Baena, F.J., Ollero Márquez, F.J., Cerro Sánchez, P.d. y Pérez Montaño, F.d.A. (2022). The Rhizobium tropici CIAT 899 NodD2 protein promotes symbiosis and extends rhizobial nodulation range by constitutive nodulation factor synthesis. Journal of Experimental Botany, 73 (19), 6931-6941. https://doi.org/10.1093/jxb/erac325.
10.1093/jxb/erac325
https://hdl.handle.net/11441/139079
10.1093/jxb/erac325
In the symbiotic associations between rhizobia and legumes, the NodD regulators orchestrate the transcription of the
specifc nodulation genes. This set of genes is involved in the synthesis of nodulation factors, which are responsible
for initiating the nodulation process. Rhizobium tropici CIAT 899 is the most successful symbiont of Phaseolus vulgaris and can nodulate a variety of legumes. Among the fve NodD regulators present in this rhizobium, only NodD1
and NodD2 seem to have a role in the symbiotic process. However, the individual role of each NodD in the absence of
the other proteins has remained elusive. In this work, we show that the CIAT 899 NodD2 does not require activation by
inducers to promote the synthesis of nodulation factors. A CIAT 899 strain overexpressing nodD2, but lacking all additional nodD genes, can nodulate three different legumes as effciently as the wild type. Interestingly, CIAT 899 NodD2-
mediated gain of nodulation can be extended to another rhizobial species, since its overproduction in Sinorhizobium
fredii HH103 not only increases the number of nitrogen-fxing nodules in two host legumes but also results in nodule
development in incompatible legumes. These fndings potentially open exciting opportunities to develop rhizobial
inoculants and increase legume crop production.
Spanish Ministry of Science and Innovation funded by MCIN/AEI/10.13039/501100011033 AGL2016-77163-R and PID2019- 107634RB-I00
Ministerio de Economía y Competitividad FPU18/06248
application/pdf
10 p.
eng
Oxford University Press
Journal of Experimental Botany, 73 (19), 6931-6941.
AGL2016-77163-R
PID2019
107634RB-I00
FPU18/06248
https://dx.doi.org/10.1093/jxb/erac325
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Common bean
Infection thread
Nodulation
Nodulation factor
Lotus
Rhizobium–legume symbiosis
Rhizobium tropici
The Rhizobium tropici CIAT 899 NodD2 protein promotes symbiosis and extends rhizobial nodulation range by constitutive nodulation factor synthesis
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Ministerio de Ciencia e Innovación (MICIN). España
Ministerio de Economía y Competitividad (MINECO). España
Journal of Experimental Botany
73
19
6931
6941
https://ror.org/03yxnpp24
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/139079/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
ORIGINAL
puberac325.pdf
puberac325.pdf
application/pdf
954055
https://idus.us.es/bitstream/11441/139079/1/puberac325.pdf
4bd91ccd958fa4556646472d441ad828
MD5
1
open access
11441/139079
oai:idus.us.es:11441/139079
2024-02-13 23:12:59.862
open access
idUS - Universidad de Sevilla
idus@us.es
RGVjbGFyYSBxdWU6CgphKSBFcyBhdXRvciB5IHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIHByb3BpZWRhZCBpbnRlbGVjdHVhbC4KCgpiKSBTaSBleGlzdGUgcHJldmlhIGNlc2nDs24gYSB0ZXJjZXJvcyBkZSBsb3MgZGVyZWNob3MgZGUgZXhwbG90YWNpw7NuIGRlIGxhIG9icmEsIGN1ZW50YSBjb24gbGEgYXV0b3JpemFjacOzbiBkZSBkaWNob3MgdGl0dWxhcmVzLiAKCgpjKSBTaSBlbCBkb2N1bWVudG8gY29udGllbmUgbWF0ZXJpYWxlcyBkZSBsb3MgcXVlIG5vIGVzIHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIGV4cGxvdGFjacOzbiwgIHRpZW5lIGVsIHBlcm1pc28gcGFyYSBkZXBvc2l0YXJsb3MgeSBxdWUgZXNlIG1hdGVyaWFsIGVzdMOhIGlkZW50aWZpY2FkbyBjbGFyYW1lbnRlLgoKCkVsIGF1dG9yIHJlYWxpemEgbGEgY2VzacOzbiBncmF0dWl0YSB5IG5vIGV4Y2x1c2l2YSBhIGxhIFVuaXZlcnNpZGFkIGRlIFNldmlsbGEgZGUgbG9zIGRlcmVjaG9zIGRlIHJlcHJvZHVjY2nDs24sIGRpc3RyaWJ1Y2nDs24sIGNvbXVuaWNhY2nDs24gcMO6YmxpY2EgeSB0cmFuc2Zvcm1hY2nDs247IHBlcm1pdGllbmRvIGFsIHJlcG9zaXRvcmlvOgoKCglUcmFuc2Zvcm1hY2nDs24gZGVsIGZvcm1hdG8gcGFyYSBzdSBpbmNvcnBvcmFjacOzbiB5IHByZXNlcnZhY2nDs24uCgoKCVJlcHJvZHVjY2nDs24geSBhcmNoaXZvICBlbiBsb3Mgc2Vydmlkb3JlcyBhc29jaWFkb3MgYWwgcmVwb3NpdG9yaW8uCgoKCVN1IGNvbXVuaWNhY2nDs24gcMO6YmxpY2EgeSBzdSBwdWVzdGEgYSBkaXNwb3NpY2nDs24gZGUgbW9kbyBsaWJyZSB5IGdyYXR1aXRvIGEgdHJhdsOpcyBkZWwgYXJjaGl2byBhYmllcnRvIGluc3RpdHVjaW9uYWwKCgpFbCBhdXRvciBhdXRvcml6YSBxdWUgbGEgb2JyYSBzZSBwb25nYSBhIGRpc3Bvc2ljacOzbiBkZSBsb3MgdXN1YXJpb3MgYSB0cmF2w6lzIGRlbCByZXBvc2l0b3JpbyBpbnN0aXR1Y2lvbmFsIHBhcmEgcXVlIHNlIGhhZ2EgdW4gdXNvIGp1c3RvLCByZXNwZXRhbmRvIGxvcyBkZXJlY2hvcyBkZSBhdXRvciBxdWUgbGEgbGVnaXNsYWNpw7NuIGVzdGFibGVjZSB5IGNvbmZvcm1lIGNvbiBsYXMgY29uZGljaW9uZXMgbWFyY2FkYXMgcG9yIGxhIGxpY2VuY2lhIGRlIHVzbwo=
oai:idus.us.es:11441/598552024-02-14T20:05:56Zcom_11441_11014com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11015col_11441_10904
Ballesta Mudarra, Sofía
Machuca-Portillo, Guillermo
Torres-Lagares, Daniel
Rodríguez-Caballero, Ángela
Yañez-Vico, Rosa
Solano Reina, José Enrique
Perea Pérez, Evelio José
2017-05-16T07:55:43Z
2017-05-16T07:55:43Z
2013
Ballesta Mudarra, S., Machuca Portillo, G., Torres-Lagares, D., Rodríguez-Caballero, Á., Yáñez Vico, R.M., Solano Reina, J.E. y Perea Pérez, E.J. (2013). Determination of periodontopathogens in patients with Cri du chat syndrome. Medicina Oral Patología Oral y Cirugía Bucal, 18 (6), e883-e887.
1698-4447 (papel)
1698-6946 (electrónico)
http://hdl.handle.net/11441/59855
10.4317/medoral.19400
Objectives: Cri du chat syndrome is a genetic alteration associated with some oral pathologies. However, it has not been described previously any clinical relationship between the periodontal disease and the syndrome. The purpose of this comparative study was to compare periodontopathogenic flora in a group with Cri du chat syndrome and another without the síndrome, to assess a potential microbiological predisposition to suffer a periodontitis. Study Design: The study compared nineteen subjects with Cri du chat Syndrome with a control group of nineteen patients without it. All patients were clinically evaluated by periodontal probing, valuing the pocket depth, the clinical attachmente level and bleeding on probing. There were no significant differences between both groups. Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythia and Treponema denticola were detected by multiplex-PCR using 16S rDNA (microIDENT). Results: When A. actinomycetemcomitans, P. gingivalis, P. intermedia and T. denticola were compared, no statistically significant differences were found between the two groups (p>0.05). The value of T. forsythia was significantly higher for Cri du chat syndrome (31.6%) than for the control group (5.3%). The odds ratio for T. forsythia was 8.3. Conclusions: In the present study T. forsythia is associated with Cri du chat syndrome subjects and not with healthy subjects.
Department of Stomatology (CTS-113) (CTS-353)
School of Medicine (CTS-210)
Instituto de Investigación de la Universidad de Sevilla (España)
application/pdf
eng
Medicina Oral
Medicina Oral Patología Oral y Cirugía Bucal, 18 (6), e883-e887.
(CTS-113) (CTS-353)
(CTS-210)
http://dx.doi.org/10.4317/medoral.19400
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Cri du Chat syndrome
Periodontal health
Microbiology
Determination of periodontopathogens in patients with Cri du chat syndrome
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Estomatología
Universidad de Sevilla. Departamento de Microbiología
Medicina Oral Patología Oral y Cirugía Bucal
18
6
e883
e887
https://ror.org/03yxnpp24
5 p.
ORIGINAL
determination of periodontopathogens.pdf
determination of periodontopathogens.pdf
application/pdf
420726
https://idus.us.es/bitstream/11441/59855/1/determination%20of%20periodontopathogens.pdf
6855cb510e3f1cb599f92fed35b413a8
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/59855/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/59855/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/59855
oai:idus.us.es:11441/59855
2024-02-14 21:05:56.867
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/648372024-02-14T09:25:37Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Rodríguez Beltrán, Jerónimo
Cabot, Gabriel
Ynés Valencia, Estela
Costas, Coloma
Bou, Germán
Oliver, Antonio
Blázquez, Jesús
2017-09-28T15:47:42Z
2017-09-28T15:47:42Z
2015-01-01
Rodríguez Beltrán, J., Cabot, G., Ynés Valencia, E., Costas, C., Bou, G., Oliver, A. y Blázquez, J. (2015). N-acetylcysteine selectively antagonizes the activity of imipenem in Pseudomonas aeruginosa by an OprD-mediated mechanism. Antimicrobial Agents and Chemotherapy, 59 (6), 3246-3251.
0066-4804 (impreso)
1098-6596 (electrónico)
http://hdl.handle.net/11441/64837
10.1128/AAC.00017-15
The modulating effect of N-acetylcysteine (NAC) on the activity of different antibiotics has been studied in Pseudomonas aeruginosa. Our results demonstrate that, in contrast to previous reports, only the activity of imipenem is clearly affected by NAC. MIC and checkerboard determinations indicate that the NAC-based modulation of imipenem activity is dependent mainly on OprD. SDS-PAGE of outer membrane proteins (OMPs) after NAC treatments demonstrates that NAC does not modify the expression of OprD, suggesting that NAC competitively inhibits the uptake of imipenem through OprD. Similar effects on imipenem activity were obtained with P. aeruginosa clinical isolates. Our results indicate that imipenem-susceptible P. aeruginosa strains become resistant upon simultaneous treatment with NAC and imipenem. Moreover, the generality of the observed effects of NAC on antibiotic activity was assessed with two additional bacterial species, Escherichia coli and Acinetobacter baumannii. Caution should be taken during treatments, as the activity of imipenem may be modified by physiologically attainable concentrations of NAC, particularly during intravenous and nebulized regimes.
Ministerio de Ciencia e Innovación y Instituto de Salud Carlos III y European Development Regional Fund (ERDF) y Spanish Network for Research in Infectious Diseases REIPI RD12/0015 FIS PI13/00063
application/pdf
eng
American Society for Microbiology
Antimicrobial Agents and Chemotherapy, 59 (6), 3246-3251.
REIPI RD12/0015
FIS PI13/00063
http://dx.doi.org/10.1128/AAC.00017-15
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
N-acetylcysteine selectively antagonizes the activity of imipenem in Pseudomonas aeruginosa by an OprD-mediated mechanism
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Ministerio de Ciencia e Innovación (MICIN). España
Antimicrobial Agents and Chemotherapy
59
6
3246
3251
https://ror.org/03yxnpp24
6 p.
ORIGINAL
N-acetylcysteine selectively antagonizes the activity of imipenem in Pseudomonas aeruginosa by an OprD-mediated mechanism.pdf
N-acetylcysteine selectively antagonizes the activity of imipenem in Pseudomonas aeruginosa by an OprD-mediated mechanism.pdf
application/pdf
712962
https://idus.us.es/bitstream/11441/64837/1/N-acetylcysteine%20selectively%20antagonizes%20the%20activity%20of%20imipenem%20in%20Pseudomonas%20aeruginosa%20by%20an%20OprD-mediated%20mechanism.pdf
c3aec69c6b0086c4de149d94f878ab68
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/64837/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/64837/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/64837
oai:idus.us.es:11441/64837
2024-02-14 10:25:37.491
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/480892024-02-17T17:21:45Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Cerro Sánchez, Pablo del
Rolla-Santos, Amanda Alves Paiva
Gomes, Douglas Fabiano
Berquó Marks, Bettina
Espuny Gómez, María del Rosario
Rodríguez Carvajal, Miguel Ángel
Soria Díaz, María Eugenia
Shigueyoshi Nakatani, André
Hungria, Mariangela
Ollero Márquez, Francisco Javier
Megías Guijo, Manuel
2016-10-25T10:51:10Z
2016-10-25T10:51:10Z
2015
Cerro Sánchez, P.d., Rolla-Santos, A.A.P., Gomes, D.F., Berquó Marks, B., Espuny Gómez, M.d.R., Rodríguez Carvajal, M.Á.,...,Megías Guijo, M. (2015). Opening the "black box" of nodD3, nodD4 and nodD5 genes of Rhizobium tropici strain CIAT 899. BMC Genomics, 16 (1), 1-10.
1471-2164
http://hdl.handle.net/11441/48089
10.1186/s12864-015-2033-z
https://idus.us.es/xmlui/handle/11441/48089
Background: Transcription of nodulation genes in rhizobial species is orchestrated by the regulatory nodD gene. Rhizobium tropici strain CIAT 899 is an intriguing species in possessing features such as broad host range, high tolerance of abiotic stresses and, especially, by carrying the highest known number of nodD genes-five-and the greatest diversity of Nod factors (lipochitooligosaccharides, LCOs). Here we shed light on the roles of the multiple nodD genes of CIAT 899 by reporting, for the first time, results obtained with nodD3, nodD4 and nodD5 mutants. Methods: The three nodD mutants were built by insertion of Ω interposon. Nod factors were purified and identified by LC-MS/MS analyses. In addition, nodD1 and nodC relative gene expressions were measured by quantitative RT-PCR in the wt and derivative mutant strains. Phenotypic traits such as exopolysaccharide (EPS), lipopolysaccharide (LPS), swimming and swarming motilities, biofilm formation and indole acetid acid (IAA) production were also perfomed. All these experiments were carried out in presence of both inducers of CIAT 899, apigenin and salt. Finally, nodulation assays were evaluated in up to six different legumes, including common bean (Phaseolus vulgaris L.). Results: Phenotypic and symbiotic properties, Nod factors and gene expression of nodD3, nodD4 and nodD5 mutants were compared with those of the wild-type (WT) CIAT 899, both in the presence and in the absence of the nod-gene-inducing molecule apigenin and of saline stress. No differences between the mutants and the WT were observed in exopolysaccharide (EPS) and lipopolysaccharide (LPS) profiles, motility, indole acetic acid (IAA) synthesis or biofilm production, either in the presence, or in the absence of inducers. Nodulation studies demonstrated the most complex regulatory system described so far, requiring from one (Leucaena leucocephala, Lotus burtii) to four (Lotus japonicus) nodD genes. Up to 38 different structures of Nod factors were detected, being higher under salt stress, except for the nodD5 mutant; in addition, a high number of structures was synthesized by the nodD4 mutant in the absence of any inducer. Probable activator (nodD3 and nodD5) or repressor roles (nodD4), possibly via nodD1 and/or nodD2, were attributed to the three nodD genes. Expression of nodC, nodD1 and each nodD studied by RT-qPCR confirmed that nodD3 is an activator of nodD1, both in the presence of apigenin and salt stress. In contrast, nodD4 might be an inducer with apigenin and a repressor under saline stress, whereas nodD5 was an inducer under both conditions. Conclusions: We report for R. tropici CIAT 899 the most complex model of regulation of nodulation genes described so far. Five nodD genes performed different roles depending on the host plant and the inducing environment. Nodulation required from one to four nodD genes, depending on the host legume. nodD3 and nodD5 were identified as activators of the nodD1 gene, whereas, for the first time, it was shown that a regulatory nodD gene-nodD4-might act as repressor or inducer, depending on the inducing environment, giving support to the hypothesis that nodD roles go beyond nodulation, in terms of responses to abiotic stresses
application/pdf
eng
BioMed Central
BMC Genomics, 16 (1), 1-10.
10.1186/s12864-015-2033-z
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Biological nitrogen fixation
LCO
Nod factors
NodD gene
Symbiosis
Opening the "black box" of nodD3, nodD4 and nodD5 genes of Rhizobium tropici strain CIAT 899
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
BMC Genomics
16
1
1
10
https://ror.org/03yxnpp24
10 p.
ORIGINAL
Opening the black box.pdf
Opening the black box.pdf
application/pdf
945547
https://idus.us.es/bitstream/11441/48089/1/Opening%20the%20black%20box.pdf
0836be44b4d25e5eff41f01752b5d466
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/48089/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/48089/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/48089
oai:idus.us.es:11441/48089
2024-02-17 18:21:45.413
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1416372023-01-20T12:00:30Zcom_11441_10903com_11441_10802com_11441_10690com_11441_10923col_11441_10904col_11441_10924
Fuentes Romero, Francisco
Moyano Bravo, Isamar
Ayala García, Paula
Rodríguez Carvajal, Miguel Ángel
Pérez Montaño, Francisco de Asís
Acosta Jurado, Sebastián
Ollero Márquez, Francisco Javier
Vinardell González, José María
2023-01-20T12:00:30Z
2023-01-20T12:00:30Z
2023
Fuentes Romero, F., Moyano Bravo, I., Ayala García, P., Rodríguez Carvajal, M.Á., Pérez Montaño, F.d.A., Acosta Jurado, S.,...,Vinardell González, J.M. (2023). Non-Ionic Osmotic Stress Induces the Biosynthesis of Nodulation Factors and Affects Other Symbiotic Traits in Sinorhizobium fredii HH103. Biology, 12 (2), 148. https://doi.org/10.3390/biology12020148.
2079-7737
https://hdl.handle.net/11441/141637
10.3390/biology12020148
Simple Summary: Rhizobia are soil proteobacteria able to establish nitrogen-fixing symbiosis with host legumes. This symbiotic interaction, which is highly important from ecological and agronomical points of view since it allows growth of legumes in soils poor in nitrogen, requires a complex interchange of molecular signals between both symbionts. The production of rhizobial molecular signals is elicited by flavonoids, which are phenolic compounds exuded by legume roots. Recent work has shown that osmotic stress can also promote the formation of symbiotic signals in some rhizobia. In this work, we show that this is also the case for Sinorhizobium fredii HH103, a rhizobial strain able to establish symbiosis with hundreds of legumes, including the very important crop, soybean. Non-ionic osmotic stress, which can be encountered by the bacterium in the rhizosphere or inside the legume host, affected the expression of hundreds of bacterial genes and, consequently, influenced diverse bacterial traits, including the production of symbiotic signals and certain characteristics that may be relevant for successful interaction with the host: motility, production of the phytohormone indole acetic acid, and production of molecules involved in bacterial cell-to-cell communication. Thus, our work provides new evidence of how stress can promote rhizobia-legume symbiosis.
Abstract: (1) Background: Some rhizobia, such as Rhizobium tropici CIAT 899, activate nodulation genes when grown under osmotic stress. This work aims to determine whether this phenomenon also takes place in Sinorhizobium fredii HH103. (2) Methods: HH103 was grown with and without 400 mM mannitol. β-galactosidase assays, nodulation factor extraction, purification and identification by mass spectrometry, transcriptomics by RNA sequencing, motility assays, indole acetic acid quantification, analysis of acyl-homoserine lactones, and indole acetic acid quantification were performed. (3) Results: Non-ionic osmotic stress induced the production of nodulation factors. Fortytwo different factors were detected, compared to 14 found in the absence of mannitol. Transcriptomics indicated that hundreds of genes were either activated or repressed upon non-ionic osmotic stress. The presence of 400 mM mannitol induced the production of indole acetic acid and acyl homoserine lactones, abolished swimming, and promoted surface motility. (4) Conclusions: In this work, we show that non-ionic stress in S. fredii HH103, caused by growth in the presence of 400 mM mannitol, provokes notable changes not only in gene expression but also in various bacterial traits, including the production of nodulation factors and other symbiotic signals.
Ministerio de Ciencia, Innovación y Universidades de España-PID2019-107634RB-I00
Fondos FEDER de la UE y Universidad de Sevilla-US-1250546
application/pdf
22 p.
eng
MDPI
Biology, 12 (2), 148.
PID2019-107634RB-I00
US-1250546
https://doi.org/10.3390/biology12020148
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Sinorhizobium fredii
osmotic stress
rhizobia-legume symbiosis
transcriptomics
Nod factors
indole acetic acid
bacterial motility
acyl-homoserine lactones
Non-Ionic Osmotic Stress Induces the Biosynthesis of Nodulation Factors and Affects Other Symbiotic Traits in Sinorhizobium fredii HH103
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Química orgánica
Ministerio de Ciencia, Innovación y Universidades (MICINN). España
European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
Universidad de Sevilla
Biology
12
2
148
ORIGINAL
biology-12-00148.pdf
biology-12-00148.pdf
application/pdf
2391775
https://idus.us.es/bitstream/11441/141637/1/biology-12-00148.pdf
1448ddf9ac8fdf26dfe4fbdf912a5661
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/141637/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/141637
oai:idus.us.es:11441/141637
2023-01-20 13:00:30.617
open access
idUS - Universidad de Sevilla
idus@us.es
RGVjbGFyYSBxdWU6CgphKSBFcyBhdXRvciB5IHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIHByb3BpZWRhZCBpbnRlbGVjdHVhbC4KCgpiKSBTaSBleGlzdGUgcHJldmlhIGNlc2nDs24gYSB0ZXJjZXJvcyBkZSBsb3MgZGVyZWNob3MgZGUgZXhwbG90YWNpw7NuIGRlIGxhIG9icmEsIGN1ZW50YSBjb24gbGEgYXV0b3JpemFjacOzbiBkZSBkaWNob3MgdGl0dWxhcmVzLiAKCgpjKSBTaSBlbCBkb2N1bWVudG8gY29udGllbmUgbWF0ZXJpYWxlcyBkZSBsb3MgcXVlIG5vIGVzIHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIGV4cGxvdGFjacOzbiwgIHRpZW5lIGVsIHBlcm1pc28gcGFyYSBkZXBvc2l0YXJsb3MgeSBxdWUgZXNlIG1hdGVyaWFsIGVzdMOhIGlkZW50aWZpY2FkbyBjbGFyYW1lbnRlLgoKCkVsIGF1dG9yIHJlYWxpemEgbGEgY2VzacOzbiBncmF0dWl0YSB5IG5vIGV4Y2x1c2l2YSBhIGxhIFVuaXZlcnNpZGFkIGRlIFNldmlsbGEgZGUgbG9zIGRlcmVjaG9zIGRlIHJlcHJvZHVjY2nDs24sIGRpc3RyaWJ1Y2nDs24sIGNvbXVuaWNhY2nDs24gcMO6YmxpY2EgeSB0cmFuc2Zvcm1hY2nDs247IHBlcm1pdGllbmRvIGFsIHJlcG9zaXRvcmlvOgoKCglUcmFuc2Zvcm1hY2nDs24gZGVsIGZvcm1hdG8gcGFyYSBzdSBpbmNvcnBvcmFjacOzbiB5IHByZXNlcnZhY2nDs24uCgoKCVJlcHJvZHVjY2nDs24geSBhcmNoaXZvICBlbiBsb3Mgc2Vydmlkb3JlcyBhc29jaWFkb3MgYWwgcmVwb3NpdG9yaW8uCgoKCVN1IGNvbXVuaWNhY2nDs24gcMO6YmxpY2EgeSBzdSBwdWVzdGEgYSBkaXNwb3NpY2nDs24gZGUgbW9kbyBsaWJyZSB5IGdyYXR1aXRvIGEgdHJhdsOpcyBkZWwgYXJjaGl2byBhYmllcnRvIGluc3RpdHVjaW9uYWwKCgpFbCBhdXRvciBhdXRvcml6YSBxdWUgbGEgb2JyYSBzZSBwb25nYSBhIGRpc3Bvc2ljacOzbiBkZSBsb3MgdXN1YXJpb3MgYSB0cmF2w6lzIGRlbCByZXBvc2l0b3JpbyBpbnN0aXR1Y2lvbmFsIHBhcmEgcXVlIHNlIGhhZ2EgdW4gdXNvIGp1c3RvLCByZXNwZXRhbmRvIGxvcyBkZXJlY2hvcyBkZSBhdXRvciBxdWUgbGEgbGVnaXNsYWNpw7NuIGVzdGFibGVjZSB5IGNvbmZvcm1lIGNvbiBsYXMgY29uZGljaW9uZXMgbWFyY2FkYXMgcG9yIGxhIGxpY2VuY2lhIGRlIHVzbwo=
oai:idus.us.es:11441/1360712024-03-15T10:47:44Zcom_11441_10818com_11441_10802com_11441_10690com_11441_10903col_11441_10819col_11441_10904
Osa Fernández, Clara de la
Pérez López, Jesús
Feria Bourrellier, Ana Belén
Baena Vaca, Guillermo
Marino, Daniel
Coleto, Inmaculada
Pérez Montaño, Francisco de Asís
Gandullo Tovar, Jacinto Manuel
Echevarría Ruiz de Vargas, Cristina
García-Mauriño Ruiz-Berdejo, Sofía
Monreal Hermoso, José Antonio
2022-08-09T09:24:28Z
2022-08-09T09:24:28Z
2022
Osa Fernández, C.d.l., Pérez López, J., Feria Bourrellier, A.B., Baena Vaca, G., Marino, D., Coleto, I.,...,Monreal Hermoso, J.A. (2022). Knock-down of phosphoenolpyruvate carboxylase 3 negatively impacts growth, productivity, and responses to salt stress in sorghum (Sorghum bicolor L.). Plant Journal, 111 (1), 231-249.
1365-313X
https://hdl.handle.net/11441/136071
10.1111/tpj.15789
Phosphoenolpyruvate carboxylase (PEPC) is a carboxylating enzyme with important roles in plant metabo-lism. Most studies in C4plants have focused on photosynthetic PEPC, but less is known about non-photosynthetic PEPC isozymes, especially with respect to their physiological functions. In this work, weanalyzed the precise roles of the sorghum (Sorghum bicolor) PPC3 isozyme by the use of knock-down lineswith the SbPPC3gene silenced (Ppc3lines).Ppc3plants showed reduced stomatal conductance and plantsize, a delay in flowering time, and reduced seed production. In addition, silenced plants accumulated stressindicators such as Asn, citrate, malate, and sucrose in roots and showed higher citrate synthase activity,even in control conditions. Salinity further affected stomatal conductance and yield and had a deeperimpact on central metabolism in silenced plants compared to wild type, more notably in roots, withPpc3plants showing higher nitrate reductase and NADH-glutamate synthase activity in roots and the accumula-tion of molecules with a higher N/C ratio. Taken together, our results show that although SbPPC3 is pre-dominantly a root protein, its absence causes deep changes in plant physiology and metabolism in rootsand leaves, negatively affecting maximal stomatal opening, growth, productivity, and stress responses insorghum plants. The consequences of SbPPC3silencing suggest that this protein, and maybe orthologs inother plants, could be an important target to improve plant growth, productivity, and resistance to saltstress and other stresses where non-photosynthetic PEPCs may be implicated.
Junta de Andalucía P12-FQM-489 and PAI group BIO298
Basque Government IT932-16
Ministerio de Economíaa, Industria y Competitividad AGL2012-35708 and AGL2016-75413-P
Premio Mensual Publicación Científica Destacada de la US. Facultad de Biología
application/pdf
19 p.
eng
Wiley
Plant Journal, 111 (1), 231-249.
P12-FQM-489
PAI-BIO298
IT932-16
AGL2012-35708
AGL2016-75413-P
https://dx.doi.org/10.1111/tpj.15789
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Central metabolism
Phosphoenolpyruvate carboxylase
Productivity
RNA interference
Saltstress
Stomata
Sorghum bicolor
Knock-down of phosphoenolpyruvate carboxylase 3 negatively impacts growth, productivity, and responses to salt stress in sorghum (Sorghum bicolor L.)
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Biología Vegetal y Ecología
Universidad de Sevilla. Departamento de Microbiología
Junta de Andalucía
Gobierno Vasco
Ministerio de Economia, Industria y Competitividad (MINECO). España
Plant Journal
111
1
231
249
https://ror.org/03yxnpp24
ORIGINAL
The Plant Journal - 2022 - Osa - Knock‐down of phosphoenolpyruvate carboxylase 3 negatively impacts growth productivity .pdf
The Plant Journal - 2022 - Osa - Knock‐down of phosphoenolpyruvate carboxylase 3 negatively impacts growth productivity .pdf
application/pdf
2687364
https://idus.us.es/bitstream/11441/136071/1/The%20Plant%20Journal%20-%202022%20-%20Osa%20-%20Knock%e2%80%90down%20of%20phosphoenolpyruvate%20carboxylase%203%20negatively%20impacts%20growth%20%20productivity%20.pdf
74bf19eaa96daf1124783e18b67970ed
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/136071/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/136071
oai:idus.us.es:11441/136071
2024-03-15 11:47:44.682
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/878592024-02-14T08:56:53Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
García Palmero, Irene
Pompas Veganzones, Noemí
Villalobo Polo, Eduardo
Gioria, Sophie
Haiech, Jacques
Villalobo, Antonio
2019-07-04T15:21:24Z
2019-07-04T15:21:24Z
2017
García Palmero, I., Pompas Veganzones, N., Villalobo Polo, E., Gioria, S., Haiech, J. y Villalobo, A. (2017). The adaptors Grb10 and Grb14 are calmodulin-binding proteins. FEBS Letters, 591 (8), 1176-1186.
0014-5793
1873-3468
https://hdl.handle.net/11441/87859
10.1002/1873-3468.12623
We identified the Grb7 family members, Grb10 and Grb14, as Ca2+-dependent CaM-binding proteins using Ca2+-dependent CaM-affinity chromatography as we previously did with Grb7. The potential CaM-binding sites were identified and experimentally tested using fluorescent-labeled peptides corresponding to these sites. The apparent affinity constant of these peptides for CaM, and the minimum number of calcium ions bound to CaM that are required for effective binding to these peptides were also determined. We prepared deletion mutants of the three adaptor proteins lacking the identified sites and determined that they lost or strongly diminished their CaM-binding capacity following the sequence Grb7 > > Grb14 > Grb10. More than one CaM-binding site and/or accessory CaM-binding sites appear to exist in Grb10 and Grb14, as compared to a single one present in Grb7.
Secretaría de Estado de Investigación, Desarrollo e Innovación SAF2011-23494, SAF2014-52048-R
application/pdf
eng
Wiley-Blackwell
FEBS Letters, 591 (8), 1176-1186.
SAF2011-23494
SAF2014-52048-R
http://dx.doi.org/10.1002/1873-3468.12623
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Adaptor proteins
Calmodulin
Calmodulin-binding sites
Grb10
Grb14
Grb7
The adaptors Grb10 and Grb14 are calmodulin-binding proteins
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
FEBS Letters
591
8
1176
1186
https://ror.org/03yxnpp24
11 p.
ORIGINAL
The adaptors Grb10 and Grb14.pdf
The adaptors Grb10 and Grb14.pdf
application/pdf
398228
https://idus.us.es/bitstream/11441/87859/1/The%20adaptors%20Grb10%20and%20Grb14.pdf
027c2486c8e24ce2a13cc3fa38ab43be
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/87859/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/87859
oai:idus.us.es:11441/87859
2024-02-14 09:56:53.334
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/233462024-02-13T09:46:35Zcom_11441_10903com_11441_10802com_11441_10690com_11441_11084com_11441_10983com_11441_10923col_11441_10904col_11441_11085col_11441_10924
Rodríguez Carvajal, Miguel Ángel
Tejero Mateo, María Pilar
Espartero Sánchez, José Luis
Ruiz Sainz, José Enrique
Buendía Clavería, Ana María
Ollero Márquez, Francisco Javier
Yang, Su S.
Gil Serrano, Antonio Miguel
2015-03-03T13:35:23Z
2015-03-03T13:35:23Z
2001
1932-6203
http://www.biochemj.org/bj/357/0505/3570505.pdf
http://hdl.handle.net/11441/23346
https://idus.us.es/xmlui/handle/11441/23346
eng
PLoS ONE, 357, 505-511
Atribución-NoComercial-SinDerivadas 4.0 España
http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
Determination of the chemical structure of the capsular polysaccharide of strain B33, a fast-growing soya bean-nodulating bacterium isolated from an arid region of China
info:eu-repo/semantics/article
Universidad de Sevilla. Departamento de Química Orgánica
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica
https://ror.org/03yxnpp24
ORIGINAL
file_1.pdf
application/pdf
184344
https://idus.us.es/bitstream/11441/23346/1/file_1.pdf
7c9ba8ecd3f2f9cffabf57a3680da2e1
MD5
1
open access
11441/23346
oai:idus.us.es:11441/23346
2024-02-13 10:46:35.195
open access
idUS - Universidad de Sevilla
idus@us.es
oai:idus.us.es:11441/1068302024-02-14T08:52:18Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Durán, David
Bernal Guzmán, Patricia
Vázquez Arias, David
Blanco Romero, Esther
Garrido Sanz, Daniel
Redondo Nieto, Miguel
Rivilla, Rafael
Martín, Marta
2021-04-08T10:02:57Z
2021-04-08T10:02:57Z
2021
Durán, D., Bernal Guzmán, P., Vázquez Arias, D., Blanco Romero, E., Garrido Sanz, D., Redondo Nieto, M.,...,Martín, M. (2021). Pseudomonas fluorescens F113 type VI secretion systems mediate bacterial killing and adaption to the rhizosphere microbiome. Scientific Reports, 11, 5772.
2045-2322
https://hdl.handle.net/11441/106830
10.1038/s41598-021-85218-1
The genome of Pseudomonas fluorescens F113, a model rhizobacterium and a plant growth-promoting agent, encodes three putative type VI secretion systems (T6SSs); F1-, F2- and F3-T6SS. Bioinformatic analysis of the F113 T6SSs has revealed that they belong to group 3, group 1.1, and group 4a, respectively, similar to those previously described in Pseudomonas aeruginosa. In addition, in silico analyses allowed us to identify genes encoding a total of five orphan VgrG proteins and eight putative effectors (Tfe), some with their cognate immunity protein (Tfi) pairs. Genes encoding Tfe and Tfi are found in the proximity of P. fluorescens F113 vgrG, hcp, eagR and tap genes. RNA-Seq analyses in liquid culture and rhizosphere have revealed that F1- and F3-T6SS are expressed under all conditions, indicating that they are active systems, while F2-T6SS did not show any relevant expression under the tested conditions. The analysis of structural mutants in the three T6SSs has shown that the active F1- and F3-T6SSs are involved in interbacterial killing while F2 is not active in these conditions and its role is still unknown.. A rhizosphere colonization analysis of the double mutant affected in the F1- and F3-T6SS clusters showed that the double mutant was severely impaired in persistence in the rhizosphere microbiome, revealing the importance of these two systems for rhizosphere adaption.
España Ministerio de Ciencia, Innovación y Universidades , grant number PGC2018-093991-B-I00. EB-R
application/pdf
13 p.
eng
Nature
Scientific Reports, 11, 5772.
PGC2018-093991-B-I00. EB-R
http://dx.doi.org/10.1038/s41598-021-85218-1
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Microbiology
Bacteria
Bacterial secretion
Pseudomonas fluorescens F113 type VI secretion systems mediate bacterial killing and adaption to the rhizosphere microbiome
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología y Parasitología
Ministerio de Ciencia, Innovación y Universidades (MICINN). España
Scientific Reports
11
5772
https://ror.org/03yxnpp24
ORIGINAL
pub41598_2021_Article_85218.pdf
pub41598_2021_Article_85218.pdf
application/pdf
3037491
https://idus.us.es/bitstream/11441/106830/1/pub41598_2021_Article_85218.pdf
1daea17abc973eb5c432bd43e3fdcea8
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/106830/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/106830
oai:idus.us.es:11441/106830
2024-02-14 09:52:18.139
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1489002024-03-20T13:02:07Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Belmonte Fernández, Alejandro
Herrero Ruiz, Joaquín
Galindo Moreno, María
Limón Mortés, María Cristina
Mora Santos, María del Mar
Sáez, Carmen
Japón, Miguel A.
Tortolero García, María Dolores
Romero Portillo, Francisco
2023-09-13T14:51:16Z
2023-09-13T14:51:16Z
2023
Belmonte Fernández, A., Herrero Ruiz, J., Galindo Moreno, M., Limón Mortés, M.C., Mora Santos, M.d.M., Sáez, C.,...,Romero Portillo, F. (2023). Cisplatin-induced Cell Death Increases the degradation of the MRE11-RAD50-NBS1 Complex Through the Autophagy/lysosomal Pathway. Cell Death and Differentiation, 30 (2), 488-499. https://doi.org/10.1038/s41418-022-01100-1.
1350-9047
1476-5403
https://hdl.handle.net/11441/148900
10.1038/s41418-022-01100-1
Cisplatin and other platinum-based anticancer agents are among the most widely used chemotherapy drugs in the treatment of different types of cancer. However, it is common to find patients who respond well to treatment at first but later relapse due to the appearance of resistance to cisplatin. Among the mechanisms responsible for this phenomenon is the increase in DNA damage repair. Here, we elucidate the effect of cisplatin on the MRN (MRE11-RAD50-NBS1) DNA damage sensor complex. We found that the tumor suppressor FBXW7 is a key factor in controlling the turnover of the MRN complex by inducing its degradation through lysosomes. Inhibition of lysosomal enzymes allowed the detection of the association of FBXW7-dependent ubiquitylated MRN with LC3 and the autophagy adaptor p62/SQSTM1 and the localization of MRN in lysosomes. Furthermore, cisplatin-induced cell death increased MRN degradation, suggesting that this complex is one of the targets that favor cell death. These findings open the possibility of using the induction of the degradation of the MRN complex after genotoxic damage as a potential therapeutic strategy to eliminate tumor cells.
Ministerio de Ciencia e Innovación SAF2017-87358-C2-1/2-R, PID2020-118774RB-C21, PID2020-118774RB-C22
Junta de Andalucía 2021/BIO-211, 101024268
Premio Mensual Publicación Científica Destacada de la US. Facultad de Biología
application/pdf
12 p.
eng
Springer Nature
Cell Death and Differentiation, 30 (2), 488-499.
SAF2017-87358-C2-1/2-R
PID2020-118774RB-C21
PID2020-118774RB-C22
2021/BIO-211
2021/BIO-211
101024268
https://dx.doi.org/10.1038/s41418-022-01100-1
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Cisplatin-induced Cell Death Increases the degradation of the MRE11-RAD50-NBS1 Complex Through the Autophagy/lysosomal Pathway
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Ministerio de Ciencia e Innovación (MICIN). España
Junta de Andalucía
Cell Death and Differentiation
30
2
488
499
https://ror.org/03yxnpp24
ORIGINAL
Cisplatin-induced cell death.pdf
Cisplatin-induced cell death.pdf
application/pdf
4450595
https://idus.us.es/bitstream/11441/148900/1/Cisplatin-induced%20cell%20death.pdf
9d94dd9a1e84d52ea13448d17b496853
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1142
https://idus.us.es/bitstream/11441/148900/2/license.txt
4ccdb332745e121f04fdde82917fec0c
MD5
2
open access
11441/148900
oai:idus.us.es:11441/148900
2024-03-20 14:02:07.326
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1539502024-01-24T18:48:16Zcom_11441_11049com_11441_10983com_11441_10690com_11441_11044com_11441_10903com_11441_10802col_11441_11050col_11441_11045col_11441_10904
McConnell, Michael J.
Pérez Ordóñez, Ana
Pérez-Romero, Pilar
Valencia, R.
Lepe Jiménez, José Antonio
Vázquez-Barba, Isabel
Pachón Díaz, Jerónimo
2024-01-24T18:48:16Z
2024-01-24T18:48:16Z
2012
McConnell, M.J., Pérez Ordóñez, A., Pérez-Romero, P., Valencia, R., Lepe Jiménez, J.A., Vázquez-Barba, I. y Pachón Díaz, J. (2012). Quantitative Real-Time PCR for Detection of Acinetobacter baumannii Colonization in the Hospital Environment. Journal of Clinical Microbiology, 50 (4), 1412-1414. https://doi.org/10.1128/jcm.06566-11.
0095-1137
1098-660X
https://hdl.handle.net/11441/153950
10.1128/jcm.06566-11
A real-time PCR assay was developed for detecting the presence of Acinetobacter baumannii on hospital equipment and compared to conventional bacterial culture using 100 hospital environmental samples. The real-time PCR detected contaminated surfaces in 4 h with high sensitivity (100%) compared to conventional culture. Thirty-eight percent of samples were positive by real-time PCR and negative by bacterial culture (false positives), possibly indicating the widespread presence of bacterial DNA that is not associated with viable bacteria.
application/pdf
2 p.
eng
American Society of Microbiology
Journal of Clinical Microbiology, 50 (4), 1412-1414.
https://journals.asm.org/doi/10.1128/jcm.06566-11
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Quantitative real-time PCR
Acinetobacter baumannii colonization
Hospital environment
Quantitative Real-Time PCR for Detection of Acinetobacter baumannii Colonization in the Hospital Environment
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Medicina Preventiva y Salud Pública
Universidad de Sevilla. Departamento de Medicina
Universidad de Sevilla. Departamento de Microbiología
Journal of Clinical Microbiology
50
4
1412
1414
ORIGINAL
Quantitative Real-Time .pdf
Quantitative Real-Time .pdf
application/pdf
358776
https://idus.us.es/bitstream/11441/153950/1/Quantitative%20Real-Time%20.pdf
f277b27a1490565ff81a7c76b027fa89
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1142
https://idus.us.es/bitstream/11441/153950/2/license.txt
4ccdb332745e121f04fdde82917fec0c
MD5
2
open access
11441/153950
oai:idus.us.es:11441/153950
2024-01-24 19:48:16.921
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1434842024-02-14T19:23:13Zcom_11441_11044com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11045col_11441_10904
Ortiz Padilla, Miriam
Portillo Calderón, Inés María
Velázquez Escudero, Ana
Rodríguez-Baño, Jesús
Pascual Hernández, Álvaro
Rodríguez Martínez, José Manuel
Docobo Pérez, Fernando
2023-03-20T16:30:03Z
2023-03-20T16:30:03Z
2022
Ortiz Padilla, M., Portillo Calderón, I.M., Velázquez Escudero, A., Rodríguez Baño, J., Pascual Hernández, Á., Rodríguez Martínez, J.M. y Docobo Pérez, F. (2022). Effect of Glycerol on Fosfomycin Activity against Escherichia coli. Antibiotics, 11 (11), 1612. https://doi.org/10.3390/antibiotics11111612.
2079-6382
https://hdl.handle.net/11441/143484
10.3390/antibiotics11111612
Fosfomycin is an antimicrobial that inhibits the biosynthesis of peptidoglycan by entering the bacteria through two channels (UhpT and GlpT). Glycerol is clinically used as a treatment for elevated intracranial pressure and induces the expression of glpT in Escherichia coli. Glycerol might offer synergistic activity by increasing fosfomycin uptake. The present study evaluates the use of glycerol at physiological concentrations in combination with fosfomycin against a collection of isogenic mutants of fosfomycin-related genes in E. coli strains. Induction of fosfomycin transporters, susceptibility tests, interaction assays, and time-kill assays were performed. Our results support the notion that glycerol allows activation of the GlpT transporter, but this induction is delayed over time and is not homogeneous across the bacterial population, leading to contradictory results regarding the enhancement of fosfomycin activity. The susceptibility assays showed an increase in fosfomycin activity with glycerol in the disk diffusion assay but not in the agar dilution or broth microdilution assays. Similarly, in the time-kill assays, the effect of glycerol was absent by the emergence of fosfomycin-resistant subpopulations. In conclusion, glycerol may not be a good candidate for use as an adjuvant with fosfomycin.
Instituto de Salud Carlos III PI19/01645, REIPI RD16/0016/0001, CM20/0092
application/pdf
12 p.
eng
Multidisciplinary Digital Publishing Institute (MDPI)
Antibiotics, 11 (11), 1612.
PI19/01645
REIPI RD16/0016/0001
CM20/0092
https://doi.org/10.3390/antibiotics11111612
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Antimicrobial resistance
Fosfomycin
Optimization treatment
Effect of Glycerol on Fosfomycin Activity against Escherichia coli
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Medicina
Instituto de Salud Carlos III
Antibiotics
11
11
1612
https://ror.org/03yxnpp24
ORIGINAL
Effect of Glycerol.pdf
Effect of Glycerol.pdf
application/pdf
1863580
https://idus.us.es/bitstream/11441/143484/1/Effect%20of%20Glycerol.pdf
1f4f7d19eb5ce3fff883ca3e048aedd8
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/143484/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/143484
oai:idus.us.es:11441/143484
2024-02-14 20:23:13.519
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1410972024-02-12T21:39:47Zcom_11441_11044com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11045col_11441_10904
Ortiz Padilla, M.
Portillo Calderón, I.
De Gregorio Iaria, Belén
Blázquez, J.
Rodríguez-Baño, Jesús
Pascual Hernández, Álvaro
Rodríguez Martínez, José Manuel
Docobo Pérez, Fernando
2023-01-10T15:36:11Z
2023-01-10T15:36:11Z
2021
Ortiz Padilla, M., Portillo Calderón, I., de Gregorio Iaria, B., Blázquez, J., Rodríguez Baño, J., Pascual Hernández, Á.,...,Docobo Pérez, F. (2021). Interplay Among Different Fosfomycin Resistance Mechanisms in Klebsiella Pneumoniae. Antimicrobial Agents and Chemotherapy, 65 (3), e01911-20. https://doi.org/10.1128/AAC.01911-20.
0066-4804
1098-6596
https://hdl.handle.net/11441/141097
10.1128/AAC.01911-20
The objectives of this study were to characterize the role of the uhpT, glpT, and fosA genes in fosfomycin resistance in Klebsiella pneumoniae and evaluate the use of sodium phosphonoformate (PPF) in combination with fosfomycin. Seven clinical isolates of K. pneumoniae and the reference strain (ATCC 700721) were used, and their genomes were sequenced. DuhpT, DglpT, and DfosA mutants were constructed from two isolates and K. pneumoniae ATCC 700721. Fosfomycin susceptibility testing was done by the gradient strip method. Synergy between fosfomycin and PPF was studied by checkerboard assay and analyzed using SynergyFinder. Spontaneous fosfomycin mutant frequencies at 64 and 512mg/liter, in vitro activity using growth curves with fosfomycin gradient concentrations (0 to 256mg/liter), and time-kill assays at 64 and 307mg/liter were evaluated with and without PPF (0.623mM). The MICs of fosfomycin against the clinical isolates ranged from 16 to ≥1,024mg/liter. The addition of 0.623mM PPF reduced fosfomycin MIC between 2- and 8-fold. Deletion of fosA led to a 32-fold decrease. Synergistic activities were observed with the combination of fosfomycin and PPF (most synergistic area at 0.623mM). The lowest fosfomycin-resistant mutant frequencies were found in ΔfosA mutants, with decreases in frequency from 1.69×10-1 to 1.60×10-5 for 64mg/liter of fosfomycin. In the final growth monitoring and time-kill assays, fosfomycin showed a bactericidal effect only with the deletion of fosA and not with the addition of PPF. We conclude that fosA gene inactivation leads to a decrease in fosfomycin resistance in K. pneumoniae. The pharmacological approach using PPF did not achieve enough activity, and the effect decreased with the presence of fosfomycin-resistant mutations.
Ministerio de Economía y Competitividad PI16/01824, REIPI RD12/0015/0010, EIPI RD16/0016/0001
Junta de Andalucía PI-0044
Innovative Medicines Initiative 115523, 115620, 115737
application/pdf
10 p.
eng
American Society for Microbiology
Antimicrobial Agents and Chemotherapy, 65 (3), e01911-20.
PI16/01824
REIPI RD12/0015/0010
REIPI RD16/0016/0001
PI-0044
115523
115620
115737
http://doi.org/10.1128/AAC.01911-20
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Antimicrobial resistance
Fosfomycin
Klebsiella pneumoniae
Interplay Among Different Fosfomycin Resistance Mechanisms in Klebsiella Pneumoniae
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Medicina
Ministerio de Economía y Competitividad (MINECO). España
Junta de Andalucía
Innovative Medicines Initiative (IMI)
Antimicrobial Agents and Chemotherapy
65
3
e01911-20
https://ror.org/03yxnpp24
ORIGINAL
Interplay among different.pdf
Interplay among different.pdf
application/pdf
1343444
https://idus.us.es/bitstream/11441/141097/1/Interplay%20among%20different.pdf
04ea34b838930afd305da3ee8948d840
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/141097/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/141097
oai:idus.us.es:11441/141097
2024-02-12 22:39:47.541
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1025442024-02-13T22:28:19Zcom_11441_10903com_11441_10802com_11441_10690com_11441_10923col_11441_10904col_11441_10924
Acosta Jurado, Sebastián
Rodríguez-Navarro, Dulce Nombre
Kawaharada, Yasuyuki
Perea, Juan Fernández
Gil Serrano, Antonio Miguel
Jin, Haojie
An, Qi
Rodríguez Carvajal, Miguel Ángel
Andersen, Stig Uggerhøj
Sandal, Niels Nørgaard
Stougaard, Jens
Vinardell González, José María
Ruiz Sainz, José Enrique
2020-11-09T19:02:28Z
2020-11-09T19:02:28Z
2016
Acosta Jurado, S., Rodríguez-Navarro, D.N., Kawaharada, Y., Perea, J.F., Gil Serrano, A.M., Jin, H.,...,Ruiz Sainz, J.E. (2016). Sinorhizobium fredii HH103 invades lotus burttii by crack entry in a nod factor- and surface polysaccharide-dependent manner. Molecular Plant-Microbe Interactions, 29 (12), 925-937.
0894-0282
https://hdl.handle.net/11441/102544
10.1094/MPMI-09-16-0195-R
Sinorhizobium fredii HH103-Rifr, a broad host range rhizobial strain, induces nitrogen-fixing nodules in Lotus burttii but ineffective nodules in L. japonicus. Confocal microscopy studies showed that Mesorhizobium loti MAFF303099 and S. fredii HH103-Rifr invade L. burttii roots through infection threads or epidermal cracks, respectively. Infection threads in root hairs were not observed in L. burttii plants inoculated with S. fredii HH103-Rifr. A S. fredii HH103-Rifr nodA mutant failed to nodulate L. burttii, demonstrating that Nod factors are strictly necessary for this crack-entry mode, and a noeL mutant was also severely impaired in L. burttii nodulation, indicating that the presence of fucosyl residues in the Nod factor is symbiotically relevant. However, significant symbiotic impacts due to the absence of methylation or to acetylation of the fucosyl residue were not detected. In contrast S. fredii HH103-Rifr mutants showing lipopolysaccharide alterations had reduced symbiotic capacity, while mutants affected in production of either exopolysaccharides, capsular polysaccharides, or both were not impaired in nodulation. Mutants unable to produce cyclic glucans and purine or pyrimidine auxotrophic mutants formed ineffective nodules with L. burttii. Flagellin-dependent bacterial mobility was not required for crack infection, since HH103-Rifr fla mutants nodulated L. burttii. None of the S. fredii HH103-Rif r surface-polysaccharide mutants gained effective nodulation with L. japonicus.
Junta de Andalucía P11-CVU-7500
Ministerio de Ciencia e Innovación BIO2011-30229-C01
application/pdf
13 p.
eng
American Phytopathological Society
Molecular Plant-Microbe Interactions, 29 (12), 925-937.
P11-CVU-7500
BIO2011-30229-C01
https://doi.org/10.1094/MPMI-09-16-0195-R
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Sinorhizobium fredii HH103 invades lotus burttii by crack entry in a nod factor- and surface polysaccharide-dependent manner
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Universidad de Sevilla. Departamento de Química orgánica
Molecular Plant-Microbe Interactions
29
12
925
937
https://ror.org/03yxnpp24
ORIGINAL
Sinorhizobium fredii HH103.pdf
Sinorhizobium fredii HH103.pdf
application/pdf
1728908
https://idus.us.es/bitstream/11441/102544/1/Sinorhizobium%20fredii%20HH103.pdf
e85ee5606ab2cdd16438b3394643f56c
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/102544/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/102544
oai:idus.us.es:11441/102544
2024-02-13 23:28:19.613
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1505652024-02-14T13:56:58Zcom_11441_11044com_11441_10983com_11441_10690com_11441_10903com_11441_10802col_11441_11045col_11441_10904
Babich, Tanya
Naucler, Pontus
Valik, John Karlsson
Giske, Christian G.
Benito, Natividad
Cardona, Ruben
Rodríguez-Baño, Jesús
Cueto López, Marina de
Yahav, Dafna
2023-11-13T14:36:13Z
2023-11-13T14:36:13Z
2022
Babich, T., Naucler, P., Valik, J.K., Giske, C.G., Benito, N., Cardona, R.,...,Yahav, D. (2022). Duration of Treatment for Pseudomonas aeruginosa Bacteremia: a Retrospective Study. INFECTIOUS DISEASES AND THERAPY, 11 (4), 1505-1519. https://doi.org/10.1007/s40121-022-00657-1.
2193-8229
2193-6382
https://hdl.handle.net/11441/150565
10.1007/s40121-022-00657-1
Introduction: There is no consensus regarding
optimal duration of antibiotic therapy for
Pseudomonas aeruginosa bacteremia. We aimed
to evaluate the impact of short antibiotic
course. Methods: We present a retrospective multicen ter study including patients with P. aeruginosa
bacteremia during 2009–2015. We evaluated
outcomes of patients treated with short (6–-
10 days) versus long (11–15 days) antibiotic
courses. The primary outcome was a composite
of 30-day mortality or bacteremia recurrence
and/or persistence. Univariate and inverse
probability treatment-weighted (IPTW) adjusted multivariate analysis for the primary outcome was performed. To avoid immortal time bias,
the landmark method was used.
Results: We included 657 patients; 273
received a short antibiotic course and 384 a long
course. There was no significant difference in
baseline characteristics of patients. The com posite primary outcome occurred in 61/384
patients in the long-treatment group (16%)
versus 32/273 in the short-treatment group
(12%) (p = 0.131). Mortality accounted for
41/384 (11%) versus 25/273 (9%) of cases,
respectively. Length of hospital stay was signif icantly shorter in the short group [median
13 days, interquartile range (IQR) 9–21 days,
versus median 15 days, IQR 11–26 days,
p = 0.002]. Ten patients in the long group dis continued antibiotic therapy owing to adverse
events, compared with none in the short group.
On univariate and multivariate analyses, dura tion of therapy was not associated with the
primary outcome.
Conclusions: In this retrospective study, 6–-
10 days of antibiotic course for P. aeruginosa
bacteremia were as effective as longer courses in
terms of survival and recurrence. Shorter ther apy was associated with reduced length of stay
and less drug discontinuation.
application/pdf
15 p.
eng
SPRINGER LONDON LTD
INFECTIOUS DISEASES AND THERAPY, 11 (4), 1505-1519.
https://link.springer.com/article/10.1007/s40121-022-00657-1
Atribución-NoComercial 4.0 Internacional
http://creativecommons.org/licenses/by-nc/4.0/
info:eu-repo/semantics/openAccess
Pseudomonas aeruginosa
Bacteremia
Antibiotics
Duration
Antimicrobial stewardship
Duration of Treatment for Pseudomonas aeruginosa Bacteremia: a Retrospective Study
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Medicina
Universidad de Sevilla. Departamento de Microbiología
INFECTIOUS DISEASES AND THERAPY
11
4
1505
1519
https://ror.org/03yxnpp24
ORIGINAL
Duration of Treatment.pdf
Duration of Treatment.pdf
application/pdf
320307
https://idus.us.es/bitstream/11441/150565/1/Duration%20of%20Treatment.pdf
38762ade3a065227adea5ab881c4e55e
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1142
https://idus.us.es/bitstream/11441/150565/2/license.txt
4ccdb332745e121f04fdde82917fec0c
MD5
2
open access
11441/150565
oai:idus.us.es:11441/150565
2024-02-14 14:56:58.864
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/227572024-02-14T13:41:20Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
López Baena, Francisco Javier
Vinardell González, José María
Pérez Montaño, Francisco de Asís
Crespo Rivas, Juan Carlos
Bellogín Izquierdo, Ramón Andrés
Espuny Gómez, María del Rosario
Ollero Márquez, Francisco Javier
2015-02-25T09:56:04Z
2015-02-25T09:56:04Z
2008
1465-2080
1350-0872
http://hdl.handle.net/11441/22757
https://idus.us.es/xmlui/handle/11441/22757
In this work we show that the Sinorhizobium fredii HH103 ttsI gene is essential for the expression
of the tts genes and secretion of nodulation outer proteins (Nops). Moreover, we demonstrate
for the first time, to our knowledge, that the nod box preceding ttsI is necessary for Nops
secretion. TtsI is responsible for the transcriptional activation of nopX, nopA, rhcJ and rhcQ. We
confirm that the S. fredii HH103 ttsI gene is activated by NodD1 and repressed by NolR. In
contrast, NodD2 is not involved in the regulation of ttsI expression. Despite the dependence of
expression of both ttsI and nodA on NodD1 and flavonoids, clear differences in the capacity of
some flavonoids to activate these genes were found. The expression of the ttsI and nodA
genes was also sensitive to differences in the pH of the media. Secretion of Nops in the ttsI
mutant could not be complemented with a DNA fragment containing the ttsI gene and its nod
box, but it was restored when a plasmid harbouring the ttsI, rhcC2 and y4xK genes was
transferred to the mutant strain. The symbiotic effect of Nops secretion was host-dependent but
independent of the type of nodule formed by the host legume. Nops are beneficial in the symbiosis
with Glycine max and Glycyrrhiza uralensis, and detrimental in the case of the tropical legume
Erythrina variegata.
application/pdf
eng
Microbiology, 154: 1825–1836
10.1099/mic.0.2007/016337-0
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Regulation and symbiotic significance of nodulation outer proteins secretion in Sinorhizobium fredii HH103
info:eu-repo/semantics/article
Universidad de Sevilla. Departamento de Microbiología
https://ror.org/03yxnpp24
ORIGINAL
Regulation and symbiotic significance Perez Montaño.pdf
Regulation and symbiotic significance Perez Montaño.pdf
application/pdf
309901
https://idus.us.es/bitstream/11441/22757/1/Regulation%20and%20symbiotic%20significance%20Perez%20Monta%c3%b1o.pdf
201c0407f07decad6257fd0d6337c315
MD5
1
open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/22757/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1223
https://idus.us.es/bitstream/11441/22757/3/license.txt
580f6cd7b5af407b57550b1a08877398
MD5
3
open access
11441/22757
oai:idus.us.es:11441/22757
2024-02-14 14:41:20.336
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/1562672024-03-14T12:31:01Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Bernal Guzmán, Patricia
2024-03-14T12:31:01Z
2024-03-14T12:31:01Z
2024-03-11
Bernal Guzmán, P. (2024). How are microbes helping end hunger?. Microbial Biotechnology, 17 (3), e14432. https://doi.org/10.1111/1751-7915.14432.
1751-7907
1751-7915
https://hdl.handle.net/11441/156267
10.1111/1751-7915.14432
Spanish Minister of Science and Innovation (MCIN) MCIN/AEI/10.13039/501100011033 and European Union - PID2021-123000OB-I00, TED2021-130357B-I00, CNS2022-135585
Andalusian Knowledge Agency (AAC), Grant/Award Number: ProyExcel_00450
application/pdf
9 p.
eng
Wiley
Microbial Biotechnology, 17 (3), e14432.
PID2021-123000OB-I00
TED2021-130357B-I00
CNS2022-135585
ProyExcel_00450
https://dx.doi.org/10.1111/1751-7915.14432
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
How are microbes helping end hunger?
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Ministerio de Ciencia e Innovación (MICIN). España
Agencia Estatal de Investigación. España
European Union (UE)
Junta de Andalucía
Microbial Biotechnology
17
3
e14432
ORIGINAL
10.11111751-7915.14432.pdf
10.11111751-7915.14432.pdf
application/pdf
631768
https://idus.us.es/bitstream/11441/156267/1/10.11111751-7915.14432.pdf
ad41288592513bb287f47f854c13151c
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1142
https://idus.us.es/bitstream/11441/156267/2/license.txt
4ccdb332745e121f04fdde82917fec0c
MD5
2
open access
11441/156267
oai:idus.us.es:11441/156267
2024-03-14 13:31:01.903
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/705282024-02-14T11:32:02Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Gil Bernabé, Ana María
Romero Portillo, Francisco
Limón Mortés, María Cristina
Tortolero García, María Dolores
2018-02-22T15:40:09Z
2018-02-22T15:40:09Z
2006
Gil Bernabé, A.M., Romero Portillo, F., Limón Mortes, M.C. y Tortolero García, M.D. (2006). Protein phosphatase 2A stabilizes human securin, whose phosphorylated forms are degraded via the SCF ubiquitin ligase. Molecular and Cellular Biology, 26 (11), 4017-4027.
0270-7306
https://hdl.handle.net/11441/70528
10.1128/MCB.01904-05
Sister chromatid segregation is triggered at the metaphase-to-anaphase transition by the activation of the protease separase. For most of the cell cycle, separase activity is kept in check by its association with the inhibitory chaperone securin. Activation of separase occurs at anaphase onset, when securin is targeted for destruction by the anaphase-promoting complex or cyclosome E3 ubiquitin protein ligase. This results in the release of the cohesins from chromosomes, which in turn allows the segregation of sister chromatids to opposite spindle poles. Here we show that human securin (hSecurin) forms a complex with enzymatically active protein phosphatase 2A (PP2A) and that it is a substrate of the phosphatase, both in vitro and in vivo. Treatment of cells with okadaic acid, a potent inhibitor of PP2A, results in various hyperphosphorylated forms of hSecurin which are extremely unstable, due to the action of the Skp1/Cull/F-box protein complex ubiquitin ligase. We propose that PP2A regulates hSecurin levels by counteracting its phosphorylation, which promotes its degradation. Misregulation of this process may lead to the formation of tumors, in which overproduction of hSecurin is often observed.
Ministerio de Educación y Ciencia SAF 2002-04177-C0-0
application/pdf
eng
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Phosphatase 2A
Ubiquitin protein ligase
Neoplasm Proteins
Phosphoprotein Phosphatase
SKP Cullin F-Box Protein Ligases
EC 6.3.2.19
EC 3.1.3.16
Protein phosphatase 2A stabilizes human securin, whose phosphorylated forms are degraded via the SCF ubiquitin ligase
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Ministerio de Educación y Ciencia (MEC). España
http://dx.doi.org/10.1128/MCB.01904-05
Molecular and Cellular Biology
26
11
4017
4027
https://ror.org/03yxnpp24
11
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
804
https://idus.us.es/bitstream/11441/70528/2/license_rdf
c1efe8e24d7281448e873be30ea326ff
MD5
2
open access
ORIGINAL
protein phosphatase 2A.pdf
protein phosphatase 2A.pdf
application/pdf
395489
https://idus.us.es/bitstream/11441/70528/1/protein%20phosphatase%202A.pdf
bff560e167ce22170e6d20e4f759bcee
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/70528/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/70528
oai:idus.us.es:11441/70528
2024-02-14 12:32:02.822
open access
idUS - Universidad de Sevilla
idus@us.es
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
oai:idus.us.es:11441/879532024-02-14T13:57:08Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Pérez Montaño, Francisco de Asís
Yang, Rongzhi
Santos García, Diego
Mateus da Silva, Gustavo
Zhao, Mei
Rosenberg, Tally
Chen, Gong
2019-07-09T10:31:25Z
2019-07-09T10:31:25Z
2019
Pérez Montaño, F.d.A., Yang, R., Santos García, D., Mateus da Silva, G., Zhao, M., Rosenberg, T. y Chen, G. (2019). Complete Assembly of the Genome of an Acidovorax citrulli Strain Reveals a Naturally Occurring Plasmid in This Species. Frontiers in Microbiology, 10 (art. 1400), 1-17.
1664-302X
https://hdl.handle.net/11441/87953
10.3389/fmicb.2019.01400
Acidovorax citrulli is the causal agent of bacterial fruit blotch (BFB), a serious threat to cucurbit crop production worldwide. Based on genetic and phenotypic properties, A. citrulli strains are divided into two major groups: group I strains have been generally isolated from melon and other non-watermelon cucurbits, while group II strains are closely associated with watermelon. In a previous study, we reported the genome of the group I model strain, M6. At that time, the M6 genome was sequenced by MiSeq Illumina technology, with reads assembled into 139 contigs. Here, we report the assembly of the M6 genome following sequencing with PacBio technology. This approach not only allowed full assembly of the M6 genome, but it also revealed the occurrence of a ∼53 kb plasmid. The M6 plasmid, named pACM6, was further confirmed by plasmid extraction, Southern-blot analysis of restricted fragments and obtention of M6-derivative cured strains. pACM6 occurs at low copy numbers (average of ∼4.1 ± 1.3 chromosome equivalents) in A. citrulli M6 and contains 63 open reading frames (ORFs), most of which (55.6%) encoding hypothetical proteins. The plasmid contains several genes encoding type IV secretion components, and typical plasmid-borne genes involved in plasmid maintenance, replication and transfer. The plasmid also carries an operon encoding homologs of a Fic-VbhA toxin-antitoxin (TA) module. Transcriptome data from A. citrulli M6 revealed that, under the tested conditions, the genes encoding the components of this TA system are among the highest expressed genes in pACM6. Whether this TA module plays a role in pACM6 maintenance is still to be determined. Leaf infiltration and seed transmission assays revealed that, under tested conditions, the loss of pACM6 did not affect the virulence of A. citrulli M6. We also show that pACM6 or similar plasmids are present in several group I strains, but absent in all tested group II strains of A. citrulli.
application/pdf
eng
Frontiers Media
Frontiers in Microbiology, 10 (art. 1400), 1-17.
http://dx.doi.org/10.3389/fmicb.2019.01400
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Acidovorax citrulli
bacterial fruit blotch
SMRT sequencing
plasmid
Complete Assembly of the Genome of an Acidovorax citrulli Strain Reveals a Naturally Occurring Plasmid in This Species
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Frontiers in Microbiology
10
art. 1400
1
17
https://ror.org/03yxnpp24
17 p.
ORIGINAL
pubfmicb-10-01400.pdf
pubfmicb-10-01400.pdf
application/pdf
2998276
https://idus.us.es/bitstream/11441/87953/1/pubfmicb-10-01400.pdf
52e02cfeda7bec1c7c0f8e2da72a854c
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/87953/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/87953
oai:idus.us.es:11441/87953
2024-02-14 14:57:08.709
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1447132024-02-17T17:51:49Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Poblete-Castro, Ignacio
Aravena-Carrasco, Carla
Orellana-Sáez, Matías
Pacheco, Nicolás
Cabrera, Álex
Borrero de Acuña, José Manuel
2023-04-20T15:49:16Z
2023-04-20T15:49:16Z
2020
2296-4185
https://hdl.handle.net/11441/144713
10.3389/fbioe.2020.00161
In the last decade, the development of novel programmable cell lytic systems based on different inducible genetic constructs like the holin–endolysin and lysozyme appears as a promising alternative to circumvent the use of costly enzymes and mechanical disrupters for downstream processing of intracellular microbial products. Despite the advances, upon activation of these systems the cellular disruption of the biocatalyst occurs in an extended period, thus delaying the recovery of poly(3-hydroxyalkanoate) (PHA). Herein the osmotic state of Pseudomonas putida KT2440 was engineered by inactivating the inner-membrane residing rescue valve MscL, which is responsible mainly for circumventing low-osmolarity challenges. Then the major outer membrane porin OprF and the specific porin OprE were overproduced during PHA producing conditions on decanoate-grown cells. The engineered P. putida strains carrying each porin showed no impairment on growth rate and final biomass and PHA yield after 48 h cultivation. Expression of both porins in tandem in the mutant strain KTΔmscL-oprFE led to a slight reduction of the biomass synthesis (∼10%) but higher PHA accumulation (%wt) relative to the cell dry mass. Each strain was then challenged to an osmotic upshift for 1 h and subsequently to a rapid passage to a hypotonic condition where the membrane stability of the KTΔmscL-oprFE suffered damage, resulting in a rapid reduction of cell viability. Cell disruption accounted for >95% of the cell population within 3 h as reported by colony forming units (CFU), FACS analyses, and transmission electron microscopy. PHA recovery yielded 94.2% of the biosynthesized biopolymer displaying no significant alterations on the final monomer composition. This study can serve as an efficient genetic platform for the recovery of any microbial intracellular compound allowing less unit operation steps for cellular disruption.
application/pdf
eng
Frontiers Research Foundation
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Pseudomonas putida
Cell lysis
Porins
MscL
Osmotic stress
Poly(3-hydroxyalkanoates)
PHA recovery
Engineering the Osmotic State of Pseudomonas putida KT2440 for Efficient Cell Disruption and Downstream Processing of Poly(3-Hydroxyalkanoates)
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
http://doi.org/10.3389/fbioe.2020.00161
Frontiers in Bioengineering and Biotechnology
8
161
https://ror.org/03yxnpp24
ORIGINAL
320.pdf
320.pdf
application/pdf
6369442
https://idus.us.es/bitstream/11441/144713/1/320.pdf
b4f563bd1599b8863ec33997495bd8b6
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1138
https://idus.us.es/bitstream/11441/144713/2/license.txt
d037f995682d8bf558422b812ec0f36c
MD5
2
open access
11441/144713
oai:idus.us.es:11441/144713
2024-02-17 18:51:49.229
open access
idUS - Universidad de Sevilla
idus@us.es
RGVjbGFyYSBxdWU6CgphKSBFcyBhdXRvciB5IHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIHByb3BpZWRhZCBpbnRlbGVjdHVhbC4KCgpiKSBTaSBleGlzdGUgcHJldmlhIGNlc2nDs24gYSB0ZXJjZXJvcyBkZSBsb3MgZGVyZWNob3MgZGUgZXhwbG90YWNpw7NuIGRlIGxhIG9icmEsIGN1ZW50YSBjb24gbGEgYXV0b3JpemFjacOzbiBkZSBkaWNob3MgdGl0dWxhcmVzLiAKCgpjKSBTaSBlbCBkb2N1bWVudG8gY29udGllbmUgbWF0ZXJpYWxlcyBkZSBsb3MgcXVlIG5vIGVzIHRpdHVsYXIgZGUgbG9zIGRlcmVjaG9zIGRlIGV4cGxvdGFjacOzbiwgIHRpZW5lIGVsIHBlcm1pc28gcGFyYSBkZXBvc2l0YXJsb3MgeSBxdWUgZXNlIG1hdGVyaWFsIGVzdMOhIGlkZW50aWZpY2FkbyBjbGFyYW1lbnRlLgoKCkVsIGF1dG9yIHJlYWxpemEgbGEgY2VzacOzbiBncmF0dWl0YSB5IG5vIGV4Y2x1c2l2YSBhIGxhIFVuaXZlcnNpZGFkIGRlIFNldmlsbGEgZGUgbG9zIGRlcmVjaG9zIGRlIHJlcHJvZHVjY2nDs24sIGRpc3RyaWJ1Y2nDs24sIGNvbXVuaWNhY2nDs24gcMO6YmxpY2EgeSB0cmFuc2Zvcm1hY2nDs247IHBlcm1pdGllbmRvIGFsIHJlcG9zaXRvcmlvOgoKCglUcmFuc2Zvcm1hY2nDs24gZGVsIGZvcm1hdG8gcGFyYSBzdSBpbmNvcnBvcmFjacOzbiB5IHByZXNlcnZhY2nDs24uCgoKCVJlcHJvZHVjY2nDs24geSBhcmNoaXZvICBlbiBsb3Mgc2Vydmlkb3JlcyBhc29jaWFkb3MgYWwgcmVwb3NpdG9yaW8uCgoKCVN1IGNvbXVuaWNhY2nDs24gcMO6YmxpY2EgeSBzdSBwdWVzdGEgYSBkaXNwb3NpY2nDs24gZGUgbW9kbyBsaWJyZSB5IGdyYXR1aXRvIGEgdHJhdsOpcyBkZWwgYXJjaGl2byBhYmllcnRvIGluc3RpdHVjaW9uYWwuCgoKRWwgYXV0b3IgYXV0b3JpemEgcXVlIGxhIG9icmEgc2UgcG9uZ2EgYSBkaXNwb3NpY2nDs24gZGUgbG9zIHVzdWFyaW9zIGEgdHJhdsOpcyBkZWwgcmVwb3NpdG9yaW8gaW5zdGl0dWNpb25hbCBwYXJhIHF1ZSBzZSBoYWdhIHVuIHVzbyBqdXN0bywgcmVzcGV0YW5kbyBsb3MgZGVyZWNob3MgZGUgYXV0b3IgcXVlIGxhIGxlZ2lzbGFjacOzbiBlc3RhYmxlY2UgeSBjb25mb3JtZSBjb24gbGFzIGNvbmRpY2lvbmVzIG1hcmNhZGFzIHBvciBsYSBsaWNlbmNpYSBkZSB1c28uCg==
oai:idus.us.es:11441/650152024-02-14T19:15:10Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Otero, Jesús
Conejo Gonzalo, Mª Carmen
González López, Juan José
Ortega, Adriana
Bou, Germán
Cercenado, Emilia
Martínez Martínez, Luis
Navarro, Ferrán
2017-10-04T17:04:30Z
2017-10-04T17:04:30Z
2014-01-01
Otero, J., Conejo Gonzalo, M.C., González López, J.J., Ortega, A., Bou, G., Cercenado, E.,...,Navarro, F. (2014). Inhibitor-resistant TEM- And OXA-1-producing Escherichia coli isolates resistant to amoxicillin-clavulanate are more clonal and possess lower virulence gene content than susceptible clinical isolates. Antimicrobial Agents and Chemotherapy, 58 (7), 3874-3881.
0066-4804 (impreso)
1098-6596 (electrónico)
http://hdl.handle.net/11441/65015
10.1128/AAC.02738-13
In a previous prospective multicenter study in Spain, we found that OXA-1 and inhibitor-resistant TEM (IRT) β-lactamases constitute the most common plasmid-borne mechanisms of genuine amoxicillin-clavulanate (AMC) resistance in Escherichia coli. In the present study, we investigated the population structure and virulence traits of clinical AMC-resistant E. coli strains expressing OXA-1 or IRT and compared these traits to those in a control group of clinical AMC-susceptible E. coli isolates. All OXA-1-producing (n = 67) and IRT-producing (n = 45) isolates were matched by geographical and temporal origin to the AMC-susceptible control set (n = 56). We performed multilocus sequence typing and phylogenetic group characterization for each isolate and then studied the isolates for the presence of 49 virulence factors (VFs) by PCR and sequencing. The most prevalent clone detected was distinct for each group: group C isolates of sequence type (ST) 88 (C/ST88) were the most common in OXA-1 producers, B2/ST131 isolates were the most common in IRT producers, and B2/ST73 isolates were the most common in AMC-susceptible isolates. The median numbers of isolates per ST were 3.72 in OXA-1 producers, 2.04 in IRT producers, and 1.69 in AMC-susceptible isolates; the proportions of STs represented by one unique isolate in each group were 19.4%, 31.1%, and 48.2%, respectively. The sum of all VFs detected, calculated as a virulence score, was significantly higher in AMC-susceptible isolates than OXA-1 and IRT producers (means, 12.5 versus 8.3 and 8.2, respectively). Our findings suggest that IRT- and OXA-1-producing E. coli isolates resistant to AMC have a different and less diverse population structure than AMC-susceptible clinical E. coli isolates. The AMC-susceptible population also contains more VFs than AMC-resistant isolates.
Fondo de Investigación Sanitaria PI09/0917
Spanish Network for Research in Infectious Diseases REIPI RD12/0015
application/pdf
eng
American Society for Microbiology
Antimicrobial Agents and Chemotherapy, 58 (7), 3874-3881.
PI09/0917
REIPI RD12/0015
http://dx.doi.org/10.1128/AAC.02738-13
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Inhibitor-resistant TEM- And OXA-1-producing Escherichia coli isolates resistant to amoxicillin-clavulanate are more clonal and possess lower virulence gene content than susceptible clinical isolates
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Instituto de Salud Carlos III
Antimicrobial Agents and Chemotherapy
58
7
3874
3881
https://ror.org/03yxnpp24
8 p.
ORIGINAL
Inhibitor-resistant TEM- And OXA-1-producing Escherichia coli isolates resistant to amoxicillin-clavulanate are more clonal and possess lower virulence gene content than susceptible clinical isolates.pdf
Inhibitor-resistant TEM- And OXA-1-producing Escherichia coli isolates resistant to amoxicillin-clavulanate are more clonal and possess lower virulence gene content than susceptible clinical isolates.pdf
application/pdf
245181
https://idus.us.es/bitstream/11441/65015/1/Inhibitor-resistant%20TEM-%20And%20OXA-1-producing%20Escherichia%20coli%20isolates%20resistant%20to%20amoxicillin-clavulanate%20are%20more%20clonal%20and%20possess%20lower%20virulence%20gene%20content%20than%20susceptible%20clinical%20isolates.pdf
7eec66f5995c91a22c5493bd74dac171
MD5
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open access
CC-LICENSE
license_rdf
license_rdf
application/rdf+xml; charset=utf-8
1223
https://idus.us.es/bitstream/11441/65015/2/license_rdf
7c9ab7f006165862d8ce9ac5eac01552
MD5
2
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/65015/3/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
3
open access
11441/65015
oai:idus.us.es:11441/65015
2024-02-14 20:15:10.074
open access
idUS - Universidad de Sevilla
idus@us.es
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oai:idus.us.es:11441/1017312020-10-06T10:46:21Zcom_11441_10903com_11441_10802com_11441_10690col_11441_10904
Cerro Sánchez, Pablo del
Ayala García, Paula
Buzón García, Pablo
Castells Graells, Roger
López Baena, Francisco Javier
Ollero Márquez, Francisco Javier
Pérez Montaño, Francisco de Asís
2020-10-06T10:46:20Z
2020-10-06T10:46:20Z
2020
Cerro Sánchez, P.d., Ayala García, P., Buzón García, P., Castells Graells, R., López Baena, F.J., Ollero Márquez, F.J. y Pérez Montaño, F.d.A. (2020). OnfD, an AraC-Type Transcriptional Regulator Encoded by Rhizobium tropici CIAT 899 and Involved in Nod Factor Synthesis and Symbiosis. Applied and Environmental Microbiology, 86 (19), e01297-1320.
1098-5336
https://hdl.handle.net/11441/101731
10.1128/AEM.01297-20
Rhizobium tropici CIAT 899 is a broad-host-range rhizobial strain that establishes symbiotic interactions with legumes and tolerates different environmental stresses such as heat, acidity, or salinity. This rhizobial strain produces a wide variety of symbiotically active nodulation factors (NF) induced not only by the presence of plant-released flavonoids but also under osmotic stress conditions through the LysR-type transcriptional regulators NodD1 (flavonoids) and NodD2 (osmotic stress). However, the activation of NodD2 under high-osmotic-stress conditions remains elusive. Here, we have studied the role of a new AraC-type regulator (named as OnfD) in the symbiotic interaction of R. tropici CIAT 899 with Phaseolus vulgaris and Lotus plants. We determined that OnfD is required under salt stress conditions for the transcriptional activation of the nodulation genes and therefore the synthesis and export of NF, which are required for a successful symbiosis with P. vulgaris. Moreover, using bacterial two-hybrid analysis, we demonstrated that the OnfD and NodD2 proteins form homodimers and OnfD/NodD2 form heterodimers, which could be involved in the production of NF in the presence of osmotic stress conditions since both regulators are required for NF synthesis in the presence of salt. A structural model of OnfD is presented and discussed.
IMPORTANCE The synthesis and export of rhizobial NF are mediated by a conserved group of LysR-type regulators, the NodD proteins. Here, we have demonstrated that a non-LysR-type regulator, an AraC-type protein, is required for the transcriptional activation of symbiotic genes and for the synthesis of symbiotically active NF under salt stress conditions.
España Ministerio de Economía y Competitividad (project AGL2016-77163-R)
application/pdf
eng
American Society for Microbiology
Applied and Environmental Microbiology, 86 (19), e01297-1320.
project AGL2016-77163-R
http://dx.doi.org/10.1128/AEM.01297-20
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
AraC-type regulators
Rhizobium tropici
egumes
plant-microbe interactions
regulation of gene expression
symbiosis
transcriptional regulation
OnfD, an AraC-Type Transcriptional Regulator Encoded by Rhizobium tropici CIAT 899 and Involved in Nod Factor Synthesis and Symbiosis
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Microbiología
Ministerio de Economía y Competitividad (MINECO). España
Applied and Environmental Microbiology
86
19
e01297
1320
ORIGINAL
pubAEM.01297-20.pdf
pubAEM.01297-20.pdf
application/pdf
2254417
https://idus.us.es/bitstream/11441/101731/1/pubAEM.01297-20.pdf
ea3c7c48850c6d8b9cce39f0186d4585
MD5
1
open access
LICENSE
license.txt
license.txt
text/plain; charset=utf-8
1136
https://idus.us.es/bitstream/11441/101731/2/license.txt
5543ff3af1b1533157819c4a43dbc2a9
MD5
2
open access
11441/101731
oai:idus.us.es:11441/101731
2020-10-06 12:46:21.06
metadata only access
idUS - Universidad de Sevilla
idus@us.es
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