2024-03-28T11:38:27Zhttps://idus.us.es/oai/requestoai:idus.us.es:11441/1484762024-02-14T09:18:39Zcom_11441_10994com_11441_10983com_11441_10690com_11441_10873com_11441_10802col_11441_10995col_11441_10874
Jiménez Madrona, Enrique
Morado Díaz, Camilo José
Talaverón Aguilocho, Rocío
Tabernero, Arantxa
Pastor Loro, Ángel Manuel
Sáez, Juan C.
Rodríguez Matarredona, Esperanza
2023-08-18T10:10:47Z
2023-08-18T10:10:47Z
2023
Jiménez Madrona, E., Morado Díaz, C.J., Talaverón Aguilocho, R., Tabernero, A., Pastor Loro, Á.M., Sáez, J.C. y Rodríguez Matarredona, E. (2023). Antiproliferative effect of boldine on neural progenitor cells and on glioblastoma cells. Frontiers in Neuroscience, 17, 1211467. https://doi.org/10.3389/fnins.2023.1211467.
1662-4548
1662-453X
https://hdl.handle.net/11441/148476
10.3389/fnins.2023.1211467
The subventricular zone (SVZ) is a brain region that contains
neural stem cells and progenitor cells (NSCs/NPCs) from which new neurons
and glial cells are formed during adulthood in mammals. Recent data indicate
that SVZ NSCs are the cell type that acquires the initial tumorigenic mutation in
glioblastoma (GBM), the most aggressive form of malignant glioma. NSCs/NPCs
of the SVZ present hemichannel activity whose function has not yet been fully
elucidated. In this work, we aimed to analyze whether hemichannel-mediated
communication affects proliferation of SVZ NPCs and GBM cells.
Universidad de Sevilla - VII PPIT
application/pdf
12 p.
eng
Frontiers Media
Frontiers in Neuroscience, 17, 1211467.
US-VII PPIT
https://doi.org/10.3389/fnins.2023.1211467
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
hemichannels
neural stem cells
glioblastoma
connexins
pannexins
Antiproliferative effect of boldine on neural progenitor cells and on glioblastoma cells
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. Departamento de Bioquímica y Biología Molecular
Universidad de Sevilla
Frontiers in Neuroscience
17
1211467
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1526602024-02-14T11:14:15Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Nogales Bueno, Fátima
Cebadero Domínguez, Óscar
Romero Herrera, Inés
Rua, Rui Manuel
Carreras Sánchez, Olimpia
Ojeda Murillo, María Luisa
2023-12-18T18:10:40Z
2023-12-18T18:10:40Z
2021-04
Nogales Bueno, F., Cebadero Domínguez, Ó., Romero Herrera, I., Rua, R.M., Carreras Sánchez, O. y Ojeda Murillo, M.L. (2021). Selenite supplementation modulates the hepatic metabolic sensors AMPK and SIRT1 in binge drinking exposed adolescent rats by avoiding oxidative stress. Food and Function, 12 (7), 3022-3032. https://doi.org/10.1039/d0fo02831b.
2042-6496
2042-650X
https://hdl.handle.net/11441/152660
10.1039/d0fo02831b
Binge drinking (BD) is the main alcohol consumption pattern among teenagers. Recently, oxidative stress (OS) generated by BD exposure has been related to hepatic metabolic deregulation and cardiovascular dysfunction. This study analyzed if BD by generating oxidative stress modulates the alteration in hepatic energy homeostasis through two important regulators of energy metabolism: the NAD+-dependent sirtuin deacetylase (SIRT1) and AMP-activated protein kinase (AMPK) and if supplementation with the antioxidant selenium (Se) improves these metabolic disorders. Four groups of adolescent rats supplemented or not with Se (0.4 ppm) and exposed to intermittent i.p. BD were used. BD rats showed an increased AST/ALT ratio, total bilirubin in serum and lipid peroxidation in the liver. The BD rats also showed a higher abdominal/thoracic ratio and increased levels of TG, gluc, and chol compared to the control group, provoking an increase in mean blood pressure (MBP). This alcohol consumption pattern decreased hepatic Se deposits, cytoplasmic GPx activity, and GSH levels as well as the expressions of two metabolic sensors and the pAMPK/AMPK ratio. Se supplementation restored antioxidant parameters and decreased lipid oxidation, avoiding OS and improving the hepatic expression of pAMPK and SIRT1, contributing to the improvement of metabolic (better lipid profile and IRS-1 expression) and vascular function (lower MBP), and to the increase of hepatic functionality (lower AST/ALT ratio). All these actions decrease cardiometabolic risk factor development in the short and long term and could disrupt the relationship between BD and MS, two problems which are currently affecting adolescents.
Junta de Andalucía CTS-193
application/pdf
22 p.
eng
Royal Society of Chemistry
Food and Function, 12 (7), 3022-3032.
CTS-193
https://doi.org/10.1039/d0fo02831b
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Selenite supplementation modulates the hepatic metabolic sensors AMPK and SIRT1 in binge drinking exposed adolescent rats by avoiding oxidative stress
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Fisiología
Junta de Andalucía
Food and Function
12
7
3022
3032
https://ror.org/03yxnpp24
oai:idus.us.es:11441/979942020-06-18T10:43:53Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874oai:idus.us.es:11441/227942024-02-13T09:58:31Zcom_11441_10818com_11441_10802com_11441_10690com_11441_10833com_11441_11019com_11441_10983com_11441_10873com_11441_26072com_11441_11084col_11441_10819col_11441_10834col_11441_11020col_11441_10874col_11441_26073col_11441_11085
Fernández Arévalo, María Mercedes
Álvarez Fuentes, Josefa
Ferrero Rodríguez, Carmen
Marín Rubio, Pedro
Mate Barrero, Alfonso
Millán Jiménez, Mónica
Morales González, Julia
Peral Rubio, María José
Pichardo Sánchez, Silvia
Gutiérrez-Praena, Daniel
Vega Pérez, José Manuel
2015-02-27T10:28:40Z
2015-02-27T10:28:40Z
2010
0004-2927
http://hdl.handle.net/11441/22794
https://idus.us.es/xmlui/handle/11441/22794
La Facultad de Farmacia de la Universidad de Sevilla (US) tiene en marcha un Programa de Alumnos Tutores desde 2006/07 con el objetivo de que alumnos de cursos superiores (AATT) tutelen a alumnos de nuevo ingreso (1x3). Pretende generar una actitud responsable en los AATT y favorecerles el desarrollo de habilidades sociales, objetivos cualitativos dentro de la educación universitaria que sirven como preparación previa a su inserción en el mundo laboral. La actividad es supervisada por Profesores Tutores (1x3) que analizan la evolución de ambos grupos de alumnos. Es una supervisión activa a través de distintas vías de acción para ayudar a la consecución de objetivos, tales como entrevistas periódicas, revisión de informes, acciones de apoyo como charlas sobre técnicas de estudio, coloquios sobre salidas laborales, exposiciones de las experiencias personales de algunos alumnos recientemente egresados, gestión estratégica de búsqueda de empleo, elaboración de portafolios,…
Con respecto a la evolución del programa, el número de profesores ha crecido moderadamente llegando a una situación estable, mientras que el número de alumnos, tanto tutores como tutelados, ha crecido en un ritmo constante acorde a las restricciones indicadas. Los resultados son muy positivos, entendiéndose que el proyecto se enmarca en un contexto más cualitativo que cuantitativo y que el principal objetivo es el robustecimiento de la experiencia y asentar una dinámica de apoyo hacia los alumnos de nuevo ingreso y de planificación de tareas, tutela y responsabilidad en general de los alumnos tutores.
The Faculty of Pharmacy of the University of Seville (US) has developed a Student Mentoring Program (from 2006/07 - present). The main objective of this project is that senior students act as Mentor Students for students at their first year in the University (1x3). It aims to generate a responsible attitude in mentor students and to promote the development of social skills, qualitative goals within higher education that serve as preparation prior to their integration into the world of work.
This activity is supervised by Mentor Professors (1x3) that analyze the evolution of both groups of students. It is an active monitoring through various actions such as regular interviews, review of reports, support operations such as lectures on study skills, seminars on job opportunities, statements of personal experiences of some recently graduated students, strategic management job search, portfolio development...
With regard to the development of the program, the number of Mentor Professors has grown moderately, reaching a stable condition, while the number of students, both tutor and supervised, has grown steadily in line with the restrictions indicated. The results are very positive, considering the more qualitative than quantitative character of the project and that the main objectives are the strengthening of the experience and the establishment of a dynamic support to the new students and scheduling and general responsibility for mentor students.
application/pdf
spa
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
EEES
Acción Tutorial
Programa de Alumnos Tutores
Innovación Docente
Desarrollo de Habilidades
Cambio de Actitudes
Grupos de Trabajo
EHEA
Tutorial Action
Student Mentor Program
Teaching Innovation
Skill Development
Attitude Change
Group Work
Acción tutorial en el EEES en la Facultad de Farmacia de la US: 4 años de experiencia de un programa de alumnos tutores
Tutorial action in the EHEA at the Faculty of Pharmacy of US: 4 years of experience of a student mentoring program
info:eu-repo/semantics/article
Universidad de Sevilla. Departamento de Farmacia y Tecnología Farmacéutica
Universidad de Sevilla. Departamento de Ecuaciones Diferenciales y Análisis Numérico
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. Departamento de Biología Vegetal y Ecología
Universidad de Sevilla. Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal
Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica
https://ror.org/03yxnpp24
oai:idus.us.es:11441/932392020-02-17T12:40:45Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Rodríguez Matarredona, Esperanza
Pastor Loro, Ángel Manuel
2020-02-17T12:40:45Z
2020-02-17T12:40:45Z
2019
Rodríguez Matarredona, E. y Pastor Loro, Á.M. (2019). Extracellular Vesicle-Mediated Communication between the Glioblastoma and Its Microenvironment. Cells, 9 (1), 1-13.
2073-4409
https://hdl.handle.net/11441/93239
10.3390/cells9010096
The glioblastoma is the most malignant form of brain cancer. Glioblastoma cells use multiple ways of communication with the tumor microenvironment in order to tune it for their own benefit. Among these, extracellular vesicles have emerged as a focus of study in the last few years. Extracellular vesicles contain soluble proteins, DNA, mRNA and non-coding RNAs with which they can modulate the phenotypes of recipient cells. In this review we summarize recent findings on the extracellular vesicles-mediated bilateral communication established between glioblastoma cells and their tumor microenvironment, and the impact of this dialogue for tumor progression and recurrence.
España MINECO grant number PGC2018-094654-B-100
application/pdf
eng
MDPI
Cells, 9 (1), 1-13.
PGC2018-094654-B-100
https://doi.org/10.3390/cells9010096
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
tumor microenvironment
exosomes
microvesicles
glioma stem cells
Extracellular Vesicle-Mediated Communication between the Glioblastoma and Its Microenvironment
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Cells
9
1
1
13
13 p.
oai:idus.us.es:11441/658702024-02-15T07:26:17Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Torres Ruiz, Blas
Beltrán Matas, Pablo
Luque Laó, María Ángeles
Herrero Rama, Luis Jacinto
2017-11-09T14:28:21Z
2017-11-09T14:28:21Z
2017
Torres Ruiz, B., Beltrán Matas, P., Luque Laó, M.Á. y Herrero Rama, L.J. (2017). Excitability is increased in hippocampal CA1 pyramidal cells of Fmr1 knockout mice. Plos One, 12 (9), 1-18.
1932-6203
http://hdl.handle.net/11441/65870
10.1371/journal.pone.0185067
Fragile X syndrome (FXS) is caused by a failure of neuronal cells to express the gene encoding the fragile mental retardation protein (FMRP). Clinical features of the syndrome include intellectual disability, learning impairment, hyperactivity, seizures and anxiety. Fmr1 knockout (KO) mice do not express FMRP and, as a result, reproduce some FXS behavioral abnormalities. While intrinsic and synaptic properties of excitatory cells in various part of the brain have been studied in Fmr1 KO mice, a thorough analysis of action potential characteristics and input-output function of CA1 pyramidal cells in this model is lacking. With a view to determining the effects of the absence of FMRP on cell excitability, we studied rheobase, action potential duration, firing frequency-current intensity relationship and action potential after-hyperpolarization (AHP) in CA1 pyramidal cells of the hippocampus of wild type (WT) and Fmr1 KO male mice. Brain slices were prepared from 8- to 12-week-old mice and the electrophysiological properties of cells recorded. Cells from both groups had similar resting membrane potentials. In the absence of FMRP expression, cells had a significantly higher input resistance, while voltage threshold and depolarization voltage were similar in WT and Fmr1 KO cell groups. No changes were observed in rheobase. The action potential duration was longer in the Fmr1 KO cell group, and the action potential firing frequency evoked by current steps of the same intensity was higher. Moreover, the gain (slope) of the relationship between firing frequency and injected current was 1.25-fold higher in the Fmr1 KO cell group. Finally, AHP amplitude was significantly reduced in the Fmr1 KO cell group. According to these data, FMRP absence increases excitability in hippocampal CA1 pyramidal cells.
application/pdf
eng
Public Library of Science
Plos One, 12 (9), 1-18.
http://dx.doi.org/10.1371/journal.pone.0185067
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Action Potentials
fragile X mental retardation protein
Pyramidal Cells
Excitability is increased in hippocampal CA1 pyramidal cells of Fmr1 knockout mice
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Plos One
12
9
1
18
https://ror.org/03yxnpp24
19 p.
oai:idus.us.es:11441/962862024-02-13T09:14:32Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Nabavi, Seyed Mohammad
Universidad de Sevilla. Departamento de Fisiología
Shirooie, Samira
Nabavi, Seyed Fazel
Dehpour, Ahmad R.
Belwal, Tarun
Habtemariam, Solomon
Argüelles Castilla, Sandro
Nabavi, Seyed Mohammad
2020-05-08T09:26:08Z
2020-05-08T09:26:08Z
2018-09
Shirooie, S., Nabavi, S.F., Dehpour, A.R., Belwal, T., Habtemariam, S., Argüelles Castilla, S. y Nabavi, S.M. (2018). Targeting mTORs by omega-3 fatty acids: a possible novel therapeutic strategy for neurodegeneration?. Pharmacological Research, 135, 37-48.
1043-6618
https://hdl.handle.net/11441/96286
10.1016/j.phrs.2018.07.004
21599743
Neurodegenerative diseases (NDs) such as Parkinson's (PD), Alzheimer's (AD), Huntington's disease (HD), and amyotrophic lateral sclerosis (ALS) cause significant world-wide morbidity and mortality. To date, there is no drug of cure for these, mostly age-related diseases, although approaches in delaying the pathology and/or giving patients some symptomatic relief have been adopted for the last few decades. Various studies in recent years have shown the beneficial effects of omega-3 poly unsaturated fatty acids (PUFAs) through diverse mechanisms including anti-inflammatory effects. This review now assesses the potential of this class of compounds in NDs therapy through specific action against the mammalian target of rapamycin (mTOR) signaling pathway. The role of mTOR in neurodegenerative diseases and targeted therapies by PUFAs are discussed.
application/pdf
11 p.
eng
Elsevier
Pharmacological Research, 135, 37-48.
https://doi.org/10.1016/j.phrs.2018.07.004
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Neurodegenerative diseases
mTOR
omega-3
Docosahexaenoic acid
Eicosapentaenoic acid
Targeting mTORs by omega-3 fatty acids: a possible novel therapeutic strategy for neurodegeneration?
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Pharmacological Research
135
37
48
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1403172024-02-13T22:09:44Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Dubuisson, Nicolas
Davis López de Carrizosa, María América
Versele, Romain
Selvais, Camille M.
Noel, Laurence
Van den Bergh, P. Y. D.
Brichard, Sonia M.
Abou-Samra, Michel
2022-12-12T11:50:48Z
2022-12-12T11:50:48Z
2022
Dubuisson, N., Davis López de Carrizosa, M.A., Versele, R., Selvais, C.M., Noel, L., Van den Bergh, P.Y.D.,...,Abou-Samra, M. (2022). Inhibiting the inflammasome with MCC950 counteracts muscle pyroptosis and improves Duchenne muscular dystrophy. Frontiers in Immunology, 13, 1049076. https://doi.org/10.3389/fimmu.2022.1049076.
1664-3224
https://hdl.handle.net/11441/140317
10.3389/fimmu.2022.1049076
Background: Duchenne muscular dystrophy (DMD) is the most common inherited human myopathy. Typically, the secondary process involving severe inflammation and necrosis exacerbate disease progression. Previously, we reported that the NLRP3 inflammasome complex plays a crucial role in this disorder. Moreover, pyroptosis, a form of programmed necrotic cell death, is triggered by NLRP3 via gasdermin D (GSDMD). So far, pyroptosis has never been described either in healthy muscle or in dystrophic muscle. The aim of this study was to unravel the role of NLRP3 inflammasome in DMD and explore a potentially promising treatment with MCC950 that selectively inhibits NLRP3.
Methods: Four‐week‐old mdx mice (n=6 per group) were orally treated for 2 months with MCC950 (mdx‐T), a highly potent, specific, small-molecule inhibitor of NLRP3, and compared with untreated (mdx) and wild-type (WT) mice. In vivo functional tests were carried out to measure the global force and endurance of mice. Ex vivo biochemical and molecular analyses were performed to evaluate the pathophysiology of the skeletal muscle. Finally, in vitro tests were conducted on primary cultures of DMD human myotubes.
Results: After MCC950 treatment, mdx mice exhibited a significant reduction of inflammation, macrophage infiltration and oxidative stress (-20 to -65%, P<0.05 vs untreated mdx). Mdx‐T mice displayed considerably less myonecrosis (-54%, P<0.05 vs mdx) and fibrosis (-75%, P<0.01 vs mdx). Moreover, a more mature myofibre phenotype, characterized by larger-sized fibres and higher expression of mature myosin heavy chains 1 and 7 was observed. Mdx-T also exhibited enhanced force and resistance to fatigue (+20 to 60%, P<0.05 or less). These beneficial effects resulted from MCC950 inhibition of both active caspase-1 (-46%, P=0.075) and cleaved gasdermin D (N-GSDMD) (-42% in medium-sized-fibres, P<0.001). Finally, the anti-inflammatory action and the anti-pyroptotic effect of MCC950 were also recapitulated in DMD human myotubes.
Conclusion: Specific inhibition of the NLRP3 inflammasome can significantly attenuate the dystrophic phenotype. A novel finding of this study is the overactivation of GSDMD, which is hampered by MCC950. This ultimately leads to less inflammation and pyroptosis and to a better muscle maturation and function. Targeting NLRP3 might lead to an effective therapeutic approach for a better management of DMD.
Fund for Scientific Research de Bélgica (FNRS)-PDR/T.0026.21
application/pdf
16 p.
eng
Frontiers Media
Frontiers in Immunology, 13, 1049076.
FNRS PDR/T.0026.21
https://doi.org/10.3389/fimmu.2022.1049076
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Inhibiting the inflammasome with MCC950 counteracts muscle pyroptosis and improves Duchenne muscular dystrophy
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Fonds de la Recherche Scientifique (FNRS). Bélgica
Frontiers in Immunology
13
1049076
https://ror.org/03yxnpp24
oai:idus.us.es:11441/230382024-02-13T09:16:20Zcom_11441_10873com_11441_10802com_11441_10690com_11441_11074com_11441_10983col_11441_10874col_11441_11075
Jiménez Ridruejo, G.
Escudero González, Miguel
Gómez González, Carlos María
Pichardo, J.V.
2015-02-27T12:18:09Z
2015-02-27T12:18:09Z
1993
0214-9915
http://www.psicothema.com/pdf/2024.pdf
http://hdl.handle.net/11441/23038
https://idus.us.es/xmlui/handle/11441/23038
eng
Psicothema, 3(2), 337-348
Atribución-NoComercial-SinDerivadas 4.0 España
http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
Parametric control of saccadic eye movements demonstrated by principal component analysis
info:eu-repo/semantics/article
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. Departamento de Psicología Experimental
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1334712024-02-14T08:44:54Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Martín Calvo, Paula
Rodríguez de la Cruz, Rosa María
Pastor Loro, Ángel Manuel
2022-05-19T10:19:14Z
2022-05-19T10:19:14Z
2020
Martín Calvo, P., Rodríguez de la Cruz, R.M. y Pastor Loro, Á.M. (2020). A Single Intraventricular Injection of VEGF Leads to Long-Term Neurotrophic Effects in Axotomized Motoneurons. eNeuro, 7 (3), ENEURO.0467-19.2020.
2373-2822
https://hdl.handle.net/11441/133471
10.1523/ENEURO.0467-19.2020
Vascular endothelial growth factor (VEGF) has been recently demonstrated to induce neuroprotective and synaptotrophic effects on lesioned neurons. Hitherto, the administration of VEGF in different animal models of lesion or disease has been conducted following a chronic protocol of administration. We questioned whether a single dose of VEGF, administered intraventricularly, could induce long-term neurotrophic effects on injured motoneurons. For this purpose, we performed in cats the axotomy of abducens motoneurons and the injection of VEGF into the fourth ventricle in the same surgical session and investigated the discharge characteristics of axotomized and treated motoneurons by single-unit extracellular recordings in the chronic alert preparation. We found that injured motoneurons treated with a single VEGF application discharged with normal characteristics, showing neuronal eye position (EP) and velocity sensitivities similar to control, thereby preventing the axotomy-induced alterations. These effects were present for a prolonged period of time (50 d) after VEGF administration. By confocal immunofluorescence we also showed that the synaptic stripping that ensues lesion was not present, rather motoneurons showed a normal synaptic coverage. Moreover, we demonstrated that VEGF did not lead to any angiogenic response pointing to a direct action of the factor on neurons. In summary, a single dose of VEFG administered just after motoneuron axotomy is able to prevent for a long time the axotomy-induced firing and synaptic alterations without any associated vascular sprouting. We consider that these data are of great relevance due to the potentiality of VEGF as a therapeutic agent in neuronal lesions and diseases.
Ministerio de Economía y Competitividad-FEDER (BFU2015-64515-P)
Ministerio de Ciencia, Innovación y Universidades (PGC2018-094654- B-100)
application/pdf
15 p.
eng
Society for Neuroscience
eNeuro, 7 (3), ENEURO.0467-19.2020.
BFU2015-64515-P
PGC2018-094654-B-100
https://doi.org/10.1523/ENEURO.0467-19.2020
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
angiogenesis
injured motoneurons
neurotrophic factors
oculomotor system
synaptic stripping
A Single Intraventricular Injection of VEGF Leads to Long-Term Neurotrophic Effects in Axotomized Motoneurons
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Economía y Competitividad (MINECO). España
European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
Ministerio de Ciencia, Innovación y Universidades (MICINN). España
eNeuro
7
3
ENEURO.0467-19.2020
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1302932024-02-14T19:42:15Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Ojeda Murillo, María Luisa
Carreras Sánchez, Olimpia
Nogales Bueno, Fátima
2022-03-01T16:37:27Z
2022-03-01T16:37:27Z
2022
Ojeda Murillo, M.L., Carreras Sánchez, O. y Nogales Bueno, F. (2022). The Role of Selenoprotein Tissue Homeostasis in MetS Programming: Energy Balance and Cardiometabolic Implications. Antioxidants, 11 (2), 394.
2076-3921
https://hdl.handle.net/11441/130293
10.3390/antiox11020394
Selenium (Se) is an essential trace element mainly known for its antioxidant, an-ti-inflammatory, and anti-apoptotic properties, as it is part of the catalytic center of 25 different selenoproteins. Some of them are related to insulin resistance (IR) and metabolic syndrome (MetS) generation, modulating reactive oxygen species (ROS), and the energetic sensor AMP-activated protein kinase (AMPK); they can also regulate the nuclear transcription factor kappa-B (NF-kB), leading to changes in inflammation production. Selenoproteins are also necessary for the correct synthesis of insulin and thyroid hormones. They are also involved in endocrine central regulation of appetite and energy homeostasis, affecting growth and development. MetS, a complex metabolic disorder, can appear during gestation and lactation in mothers, leading to energetic and metabolic changes in their offspring that, according to the metabolic programming theory, will produce cardiovascular and metabolic diseases later in life. However, there is a gap concerning Se tissue levels and selenoproteins’ implications in MetS generation, which is even greater during MetS pro-gramming. This narrative review also provides an overview of the existing evidence, based on experimental research from our laboratory, which strengthens the fact that maternal MetS leads to changes in Se tissue deposits and antioxidant selenoproteins’ expression in their offspring. These changes contribute to alterations in tissues’ oxidative damage, inflammation, energy balance, and tissue function, mainly in the heart. Se imbalance also could modulate appetite and endocrine energy balance, affecting pups’ growth and development. MetS pups present a profile similar to that of diabetes type 1, which also appeared when dams were exposed to low-Se dietary supply. Maternal Se supplementation should be taken into account if, during gestation and/or lactation periods, there are suspicions of endocrine energy imbalance in the offspring, such as MetS. It could be an interesting therapy to induce heart reprogramming. However, more studies are necessary.
Junta de Andalucía 2017/CTS-193, 2019/CTS-193, 2021/CTS-193
application/pdf
31 p.
eng
Multidisciplinary Digital Publishing Institute (MDPI)
Antioxidants, 11 (2), 394.
2017/CTS-193
2019/CTS-193
2021/CTS-193
https://doi.org/10.3390/antiox11020394
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Cardiovascular disease
Fetal programming
Metabolic syndrome
Selenium
Selenoprotein
The Role of Selenoprotein Tissue Homeostasis in MetS Programming: Energy Balance and Cardiometabolic Implications
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Antioxidants
11
2
394
https://ror.org/03yxnpp24
oai:idus.us.es:11441/442772024-02-14T20:33:46Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Davis López de Carrizosa, María América
Morado Díaz, Camilo José
Morcuende Fernández, Sara R.
Rodríguez de la Cruz, Rosa María
Pastor Loro, Ángel Manuel
2016-08-08T10:55:52Z
2016-08-08T10:55:52Z
2010
Davis López de Carrizosa, M.A., Morado Díaz, C.J., Morcuende Fernández, S.R., Rodríguez de la Cruz, R.M. y Pastor Loro, Á.M. (2010). Nerve Growth Factor Regulates the Firing Patterns and Synaptic Composition of Motoneurons. Journal of Neuroscience, 30 (24), 8308-8319.
0270-6474
http://hdl.handle.net/11441/44277
http://dx.doi.org/10.1523/JNEUROSCI.0719-10.2010
https://idus.us.es/xmlui/handle/11441/44277
Target-derived neurotrophins exert powerful synaptotrophic actions in the adult brain and are involved in the regulation of different forms of synaptic plasticity. Target disconnection produces a profound synaptic stripping due to the lack of trophic support. Conse- quently, target reinnervation leads to synaptic remodeling and restoration of cellular functions. Extraocular motoneurons are unique in that they normally express the TrkA neurotrophin receptor in the adult, a feature not seen in other cranial or spinal motoneurons, except after lesions such as axotomy or in neurodegenerative diseases like amyotrophic lateral sclerosis. We investigated the effects of nerve growth factor (NGF) by retrogradely delivering this neurotrophin to abducens motoneurons of adult cats. Axotomy reduced the density of somatic boutons and the overall tonic and phasic firing modulation. Treatment with NGF restored synaptic inputs and firing modu- lation in axotomized motoneurons. When K252a, a selective inhibitor of tyrosine kinase activity, was applied to specifically test TrkA effects, the NGF-mediated restoration of synapses and firing-related parameters was abolished. Discharge variability and recruitment threshold were, however, increased by NGF compared with control or axotomized motoneurons. Interestingly, these parameters re- turned to normal following application of REX, an antibody raised against neurotrophin receptor p75 (p75 NTR). In conclusion, NGF, acting retrogradely through TrkA receptors, supports afferent boutons and regulates the burst and tonic signals correlated with eye movements. On the other hand, p75 NTR activation regulates recruitment threshold, which impacts on firing regularity. To our knowledge, this is the first report showing powerful synaptotrophic effects of NGF on motoneurons in vivo.
application/pdf
eng
Society for Neuroscience
Journal of Neuroscience, 30 (24), 8308-8319.
10.1523/JNEUROSCI.0719-10.2010
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
k 252a
Nerve growth factor
Neurotrophin receptor p75
Protein tyrosine kinase A
Rex protein
Nerve Growth Factor Regulates the Firing Patterns and Synaptic Composition of Motoneurons
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Journal of Neuroscience
30
24
8308
8319
https://ror.org/03yxnpp24
12 p.
oai:idus.us.es:11441/699392024-02-14T11:45:07Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Monck, Jonathan R.
Álvarez de Toledo Naranjo, Guillermo
Fernández, Julio M.
2018-02-02T15:37:27Z
2018-02-02T15:37:27Z
1990
Monck, J.R., Álvarez de Toledo Naranjo, G. y Fernández, J.M. (1990). Tension in secretory granule membranes causes extensive membrane transfer through the exocytotic fusion pore. Proceedings of the National Academy of Sciences of the United States of America, 87 (20), 7804-7808.
0027-8424
https://hdl.handle.net/11441/69939
10.1073/pnas.87.20.7804
or fusion to occur the repulsive forces between two interacting phospholipid bilayers must be reduced. In model systems, this can be achieved by increasing the surface tension of at least one of the membranes. However, there has so far been no evidence that the secretory granule membrane is under tension. We have been studying exocytosis by using the patch-clamp technique to measure the surface area of the plasma membrane of degranulating mast cells. When a secretory granule fuses with the plasma membrane there is a step increase in the cell surface area. Some fusion events are reversible, in which case we have found that the backstep is larger than the initial step, indicating that there is a net decrease in the area of the plasma membrane. The decrease has the following properties: (i) the magnitude is strongly dependent on the lifetime of the fusion event and can be extensive, representing as much as 40% of the initial granule surface area; (ii) the rate of decrease is independent of granule size; and (iii) the decrease is not dependent on swelling of the secretory granule matrix. We conclude that the granule membrane is under tension and that this tension causes a net transfer of membrane from the plasma membrane to the secretory granule, while they are connected by the fusion pore. The high membrane tension in the secretory granule may be the critical stress necessary for bringing about exocytotic fusion.
National Institutes of Health GM-38857
application/pdf
eng
The National Academy of Science
Proceedings of the National Academy of Sciences of the United States of America, 87 (20), 7804-7808.
http://dx.doi.org/10.1073/pnas.87.20.7804
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Capacitance flicker
Exocytosis
Mast cells
Membrane fusion
Membrane tension
Tension in secretory granule membranes causes extensive membrane transfer through the exocytotic fusion pore
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica
National Institutes of Health. United States
Proceedings of the National Academy of Sciences of the United States of America
87
20
7804
7808
https://ror.org/03yxnpp24
5
oai:idus.us.es:11441/1528852024-02-17T17:31:48Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Martín Calvo, Paula
García Hernández, Rosendo Miguel
Pastor Loro, Ángel Manuel
Rodríguez de la Cruz, Rosa María
2024-01-02T15:58:58Z
2024-01-02T15:58:58Z
2022
Martín Calvo, P., García Hernández, R.M., Pastor Loro, Á.M. y Rodríguez de la Cruz, R.M. (2022). VEGF and Neuronal Survival. Neuroscientist. https://doi.org/10.1177/10738584221120803.
1073-8584
1089-4098
https://hdl.handle.net/11441/152885
10.1177/10738584221120803
Vascular endothelial growth factor (VEGF) is well known for its angiogenic activity, but recent evidence has revealed a neuroprotective action of this factor on injured or diseased neurons. In the present review, we summarize the most relevant findings that have contributed to establish a link between VEGF deficiency and neuronal degeneration. At issue, 1) mutant mice with reduced levels of VEGF show adult-onset muscle weakness and motoneuron degeneration resembling amyotrophic lateral sclerosis (ALS), 2) administration of VEGF to different animal models of motoneuron degeneration improves motor performance and ameliorates motoneuronal degeneration, and 3) there is an association between low plasmatic levels of VEGF and human ALS. Altogether, the results presented in this review highlight VEGF as an essential motoneuron neurotrophic factor endowed with promising therapeutic potential for the treatment of motoneuron disorders.
Junta de Andalucía P20_00529
Ministerio de Ciencia e Innovación PGC2018-094654-B-100
application/pdf
46 p.
eng
SAGE
Neuroscientist.
P20_00529
PGC2018-094654-B-100
https://doi.org/10.1177/10738584221120803
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
ALS
Axotomy
Extraocular motoneurons
Neuroprotection
Neurotrophic factors
VEGF and Neuronal Survival
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Fisiología
Junta de Andalucía
Ministerio de Ciencia e Innovación (MICIN). España
Neuroscientist
https://ror.org/03yxnpp24
oai:idus.us.es:11441/475082021-03-26T18:49:55Zcom_11441_10994com_11441_10983com_11441_10690com_11441_10873com_11441_10802col_11441_10995col_11441_10874
Naranjo, José Ángel
Ayala Gómez, Antonio
Cano Rodríguez, María Mercedes
Argüelles Castilla, Sandro
Ruiz, Victoria
Muñoz Pinto, Mario Faustino
2016-10-14T10:14:56Z
2016-10-14T10:14:56Z
2015
Naranjo, J.Á., Ayala Gómez, A., Cano Rodríguez, M.M., Argüelles, S., Ruiz, V. y Muñoz, M. (2015). Treatment with mesenchymal stem cells in an animal model of parkinson´s disease. Approaches to aging control, 19, 37-44.
1885-4028
http://hdl.handle.net/11441/47508
https://idus.us.es/xmlui/handle/11441/47508
Consejería de Economía, Innovación y Ciencia, Junta de Andalucía P10-CTS-6494
application/pdf
eng
Federation of Anti-Aging Medicine Societies
Approaches to aging control, 19, 37-44.
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Treatment with mesenchymal stem cells in an animal model of parkinson´s disease
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. Departamento de Bioquímica y Biología Molecular
Junta de Andalucía
Approaches to aging control
19
37
44
8 p.
oai:idus.us.es:11441/435712024-02-14T20:07:04Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Luque Laó, María Ángeles
Torres Torrelo, Julio
Carrascal Moreno, María Livia
Torres Ruiz, Blas
Herrero Rama, Luis Jacinto
2016-07-13T11:13:49Z
2016-07-13T11:13:49Z
2011
Luque, M.A., Torres Torrelo, J., Carrascal, L., Torres, B. y Herrero Rama, L.J. (2011). GABAergic projections to the oculomotor nucleus in the goldfish (Carassius auratus). Frontiers in Neuroanatomy, FEB, 1-7.
1662-5129
http://hdl.handle.net/11441/43571
http://dx.doi.org/10.3389/fnana.2011.00007
https://idus.us.es/xmlui/handle/11441/43571
The mammalian oculomotor nucleus receives a strong γ-aminobutyric acid (GABA)ergic synaptic input, whereas such projections have rarely been reported in fish. In order to determine whether this synaptic organization is preserved across vertebrates, we investigated the GABAergic projections to the oculomotor nucleus in the goldfish by combining retrograde transport of biotin dextran amine, injected into the antidromically identified oculomotor nucleus, and GABA immunohistochemistry.The main source of GABAergic afferents to the oculomotor nucleus was the ipsilateral anterior octaval nucleus, with only a few, if any, GABAergic neurons being located in the contralateral tangential and descending nuclei of the octaval column. In mammals there is a nearly exclusive ipsilateral projection from vestibular neurons to the oculomotor nucleus via GABAergic inhibitory inputs; thus, the vestibulooculomotor GABAergic circuitry follows a plan that appears to be shared throughout the vertebrate phylogeny. The second major source of GABAergic projections was the rhombencephalic reticular formation, primarily from the medial area but, to a lesser extent, from the inferior area. A few GABAergic oculomotor projecting neurons were also observed in the ipsilateral nucleus of the medial longitudinal fasciculus. The GABAergic projections from neurons located in both the reticular formation surrounding the abducens nucleus and the nucleus of the medial reticular formation have primarily been related to the control of saccadic eye movements. Finally, all retrogradely labeled internuclear neurons of the abducens nucleus, and neurons in the cerebellum (close to the caudal lobe), were negative for GABA. These data suggest that the vestibuloocular and saccadic inhibitory GABAergic systems appear early in vertebrate phylogeny to modulate the firing properties of the oculomotor nucleus motoneurons.
Ministerio de Innovación y Ciencia BFU 2009-07867
Junta de Andalucía P08-CVI-039 y P09-CVI-4617
application/pdf
eng
Frontiers Research Foundation
Frontiers in Neuroanatomy, FEB, 1-7.
BFU 2009-07867
P08-CVI-039
P09-CVI-4617
10.3389/fnana.2011.00007
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
GABA immunohistochemistry
Oculomotor system
Saccadic eye movements
Vestibuloocular reflex
GABAergic projections to the oculomotor nucleus in the goldfish (Carassius auratus)
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Ciencia e Innovación (MICIN). España
Junta de Andalucía
Frontiers in Neuroanatomy
FEB
1
7
https://ror.org/03yxnpp24
7 p.
oai:idus.us.es:11441/1516902024-02-14T19:17:37Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
García Hernández, Rosendo Miguel
Djebari, Souhail
Vélez Ortiz, José Miguel
Rodríguez de la Cruz, Rosa María
Pastor Loro, Ángel Manuel
Benítez Temiño, Beatriz
2023-11-27T18:31:09Z
2023-11-27T18:31:09Z
2019
García Hernández, R.M., Djebari, S., Vélez Ortiz, J.M., Rodríguez de la Cruz, R.M., Pastor Loro, Á.M. y Benítez Temiño, B. (2019). Short-term plasticity after partial deafferentation in the oculomotor system. Brain Structure and Function, 224 (8), 2717-2731. https://doi.org/10.1007/s00429-019-01929-2.
1863-2653
1863-2661
https://hdl.handle.net/11441/151690
10.1007/s00429-019-01929-2
Medial rectus motoneurons are innervated by two main pontine inputs. The specific function of each of these two inputs remains to be fully understood. Indeed, selective partial deafferentation of medial rectus motoneurons, performed by the lesion of either the vestibular or the abducens input, initially induces similar changes in motoneuronal discharge. However, at longer time periods, the responses to both lesions are dissimilar. Alterations on eye movements and motoneuronal discharge induced by vestibular input transection recover completely 2 months post-lesion, whereas changes induced by abducens internuclear lesion are more drastic and permanent. Functional recovery could be due to some kind of plastic process, such as reactive synaptogenesis, developed by the remaining intact input, which would occupy the vacant synaptic spaces left after lesion. Herein, by means of confocal microscopy, immunocytochemistry and retrograde labeling, we attempt to elucidate the possible plastic processes that take place after partial deafferentation of medial rectus motoneuron. 48 h post-injury, both vestibular and abducens internuclear lesions produced a reduced synaptic coverage on these motoneurons. However, 96 h after vestibular lesion, there was a partial recovery in the number of synaptic contacts. This suggests that there was reactive synaptogenesis. This recovery was preceded by an increase in somatic neurotrophin content, suggesting a role of these molecules in presynaptic axonal sprouting. The rise in synaptic coverage might be due to terminal sprouting performed by the remaining main input, i.e., abducens internuclear neurons. The present results may improve the understanding of this apparently redundant input system.
Ministerio de Ciencia e Innovación BFU2015-64515-P
application/pdf
44 p.
eng
Springer Nature
Brain Structure and Function, 224 (8), 2717-2731.
BFU2015-64515-P
https://doi.org/10.1007/s00429-019-01929-2
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Motoneuron
Neurotrophins
Oculomotor
Synaptic plasticity
Short-term plasticity after partial deafferentation in the oculomotor system
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Ciencia e Innovación (MICIN). España
Brain Structure and Function
224
8
2717
2731
https://ror.org/03yxnpp24
oai:idus.us.es:11441/626252024-02-17T16:39:05Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Varea Varea, Emilio
Belles, María
Vidueira, Salvador
Blasco Ibáñez, José Miguel
Crespo, Carlos
Pastor Loro, Ángel Manuel
Nacher, Juan
2017-07-18T13:28:38Z
2017-07-18T13:28:38Z
2011
Varea Varea, E., Belles, M., Vidueira, S., Blasco Ibáñez, J.M., Crespo, C., Pastor Loro, Á.M. y Nacher, J. (2011). PSA-NCAM is expressed in immature, but not recently generated, neurons in the adult cat cerebral cortex layer II. Frontiers in Neuroscience, FEB, 17-.
1662-4548 (impreso)
1662-453X (electronico)
http://hdl.handle.net/11441/62625
10.3389/fnins.2011.00017
Neuronal production persists during adulthood in the dentate gyrus and the olfactory bulb, where substantial numbers of immature neurons can be found. These cells can also be found in the paleocortex layer II of adult rodents, but in this case most of them have been generated during embryogenesis. Recent reports have described the presence of similar cells, with a wider distribution, in the cerebral cortex of adult cats and primates and have suggested that they may develop into interneurons. The objective of this study is to verify this hypothesis and to explore the origin of these immature neurons in adult cats. We have analyzed their distribution using immunohistochemical analysis of the polysialylated form of the neural cell adhesion molecule (PSA-NCAM) and their phenotype using markers of mature neurons and different interneuronal populations. Additionally, we have explored the origin of these cells administering 5′bromodeoxyuridine (5′BrdU) during adulthood. Immature neurons were widely dispersed in the cerebral cortex layers II and upper III, being specially abundant in the piriform and entorhinal cortices, in the ventral portions of the frontal and temporoparietal lobes, but relatively scarce in dorsal regions, such as the primary visual areas. Only a small fraction of PSA-NCAM expressing cells in layer II expressed the mature neuronal marker NeuN and virtually none of them expressed calcium binding proteins or neuropeptides. By contrast, most, if not all of these cells expressed the transcription factor Tbr-1, specifically expressed by pallium-derived principal neurons, but not CAMKII, a marker of mature excitatory neurons. Absence of PSA-NCAM/5'BrdU colocalization suggests that, as in rats, these cells were not generated during adulthood. Together, these results indicate that immature neurons in the adult cat cerebral cortex layer II are not recently generated and that they may differentiate into principal neurons.
Ministerio de Ciencia e Innovación BFU2009-12284/BFI
application/pdf
eng
Frontiers Media S.A.
Frontiers in Neuroscience, FEB, 17-.
BFU2009-12284/BFI
http://dx.doi.org/10.3389/fnins.2011.00017
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Adult neurogenesis
Neuronal differentiation
Structural plasticity
Interneuron
Principal neuron
PSA-NCAM is expressed in immature, but not recently generated, neurons in the adult cat cerebral cortex layer II
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Ciencia e Innovación (MICIN). España
Frontiers in Neuroscience
FEB
17
https://ror.org/03yxnpp24
9 p.
oai:idus.us.es:11441/958452024-02-17T16:33:03Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Blumer, Roland
Boesmueller, Sandra E.
Gesslbauer, Bernhard
Hirtler, Lena
Candel Bormann, Daniel
Pastor Loro, Ángel Manuel
Streicher, Johannes
Mittermayr, Rainer
2020-04-27T15:39:07Z
2020-04-27T15:39:07Z
2020
Blumer, R., Boesmueller, S.E., Gesslbauer, B., Hirtler, L., Candel Bormann, D., Pastor Loro, Á.M.,...,Mittermayr, R. (2020). Structural and molecular characteristics of axons in the long head of the biceps tendon. Cell and Tissue Research, 380 (1), 43-57.
0302-766X
1432-0878
https://hdl.handle.net/11441/95845
10.1007/s00441-019-03141-4
The innervation of the long head of the biceps tendon (LHBT) is not sufficiently documented. This is a drawback since pathologies of the LHBT are a major source of shoulder pain. Thus, the study aimed to characterize structurally and molecularly nervous elements of the LHBT. The proximal part of 11 LHBTs was harvested intraoperatively. There were 8 female and 3 male specimens. Age ranged from 66 to 86 years. For structural analyses, nervous elements were viewed in the transmission electron microscope. For molecular characterization, we used general neuronal markers including antibodies against neurofilament and protein gene product 9.5 (PGP9.5) as well as specific neuronal markers including antibodies against myelin basic protein (MBP), calcitonin gene-related product (CGRP), substance P (SP), tyrosine hydroxylase (TH), and growth-associated protein 43 (GAP43). Anti-neurofilament and anti-PGP9.5 visualized the overall innervation. Anti-MBP visualized myelination, anti-CGRP and anti-SP nociceptive fibers, anti-TH sympathetic nerve fibers, and anti-GAP43 nerve fibers during development and regeneration. Immunolabeled sections were analyzed in the confocal laser scanning microscope. We show that the LHBT contains unmyelinated as well as myelinated nerve fibers which group in nerve fascicles and follow blood vessels. Manny myelinated and unmyelinated axons exhibit molecular features of nociceptive nerve fibers. Another subpopulation of unmyelinated axons exhibits molecular characteristics of sympathetic nerve fibers. Unmyelinated sympathetic fibers and unmyelinated nociceptive fibers express proteins that are found during development and regeneration. Present findings support the hypothesis that ingrowth of nociceptive fibers are the source of chronic tendon pain.
application/pdf
15 p.
eng
Springer Nature
Cell and Tissue Research, 380 (1), 43-57.
http://dx.doi.org/10.1007/s00441-019-03141-4
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Developing nerve fibers
Innervation
Nociceptive nerve fibers
Sympathetic nerve fibers
Tendon
Structural and molecular characteristics of axons in the long head of the biceps tendon
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Cell and Tissue Research
380
1
43
57
https://ror.org/03yxnpp24
oai:idus.us.es:11441/957232024-02-14T13:29:42Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Durán Martínez, Juan Manuel
Cano Rodríguez, María Mercedes
Peral Rubio, María José
Ilundáin Larrañeta, María Anunciación Ana
2020-04-24T15:26:55Z
2020-04-24T15:26:55Z
2004
Durán Martínez, J.M., Cano Rodríguez, M.M., Peral Rubio, M.J. y Ilundáin Larrañeta, M.A.A. (2004). D-mannose transport and metabolism in isolated enterocytes. Glycobiology, 14 (6), 495-500.
0959-6658
1460-2423
https://hdl.handle.net/11441/95723
10.1093/glycob/cwh059
D-mannose transport and metabolism has been studied in enterocytes isolated from chicken small intestine. In the presence of Na+, the mannose taken up by the cells either remains free, is phosphorylated, is catabolized to H2O, or becomes part of membrane components. The mannose remaining free in the cytosol is released when the cells are transferred to an ice bath. The Na+-dependent D-mannose transport is electrogenic and inhibited by ouabain and dinitrophenol; its substrate specificity differs from SGLT-1 transporter. The Glut2 transporter inhibitors phloretin and cytochalasin B added following 30-min mannose uptake reduced the previously accumulated D-mannose, whereas these two agents increased the cell to external medium 3-O-methyl-glucose (3-OMG) concentration ratio. D-mannose efflux rate from preloaded D-[2-3H]-mannose enterocytes is Na+-independent. Phloretin did not affect D-mannose efflux rate, whereas it inhibited that of 3-OMG. Neither mannose uptake nor efflux rate were affected by fructose. It is concluded that part of the mannose taken up by the enterocytes is rapidly metabolized and that enterocytes have two D-mannose transport systems: one is concentrative and Na+-dependent and the other is Na+-independent and passive.
Dirección General de Investiagación Científica y Técnica PM99-0121
application/pdf
6 p.
eng
Oxford University Press
Glycobiology, 14 (6), 495-500.
PM99-0121
http://dx.doi.org/10.1093/glycob/cwh059
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
D-mannose
Enterocytes
Intestine
D-mannose transport and metabolism in isolated enterocytes
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Fisiología
Glycobiology
14
6
495
500
https://ror.org/03yxnpp24
oai:idus.us.es:11441/323942024-02-13T09:49:12Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Ilundáin Larrañeta, María Anunciación Ana
Núñez Abades, Pedro Antonio
Calonge Castrillo, María Luisa
Bolufer González, José
2016-01-12T09:07:57Z
2016-01-12T09:07:57Z
1999
Ilundáin Larrañeta, M.A., Núñez Abades, P.A., Calonge Castrillo, M.L. y Bolufer González, J. (1999). Utilización del refuerzo positivo como herramienta para la mejora del aprendizaje en la asignatura de Fisiología Celular. Revista de Enseñanza Universitaria, extra 1999, 159-171.
1132-8993
http://hdl.handle.net/11441/32394
https://idus.us.es/xmlui/handle/11441/32394
Este trabajo describe la actividad docente desarrollada por los profesores de la asignatura de Fisiología Celular de la Facultad de Farmacia, durante el Curso Académico 1997-98, coincidiendo con la implantación del Nuevo Plan de Estudios de la Licenciatura de Farmacia. El desarrollo de la aplicación de la actividad tuvo como objetivo aplicar nuevas estrategias de enseñanza que mejorasen a) el nivel de participación y motivación de los alumnos en la asignatura a lo largo del curso académico y b) las calificaciones obtenidas por los alumnos. Así mismo, pretendíamos evaluar el proceso de aprendizaje.
This work describes the Activity of Teaching Innovation developed by the professors of the subject Cellular Physiology, of the Faculty of Pharmacy, University of Seville, coinciding with the onset of thc New Plan of Studies of Pharmacy, during the academic course 1997-1998. The aim of thc activity was to apply new strategies in teaching to improve: a) the leve! of participation and motivation of the students in the subject throughout the academic course and b) the score obtained by the students. Furthermore, we tried to evaluate the leaming process.
application/pdf
spa
Revista de Enseñanza Universitaria, extra 1999, 159-171.
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Utilización del refuerzo positivo como herramienta para la mejora del aprendizaje en la asignatura de Fisiología Celular
info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
Universidad de Sevilla. Departamento de Fisiología
Revista de Enseñanza Universitaria
extra 1999
159
171
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1521932024-02-14T09:22:07Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Gallego López, María del Carmen
Ojeda Murillo, María Luisa
Romero Herrera, Inés
Gomes de Figueredo Rua, Rui Manuel
Carreras Sánchez, Olimpia
Nogales Bueno, Fátima
2023-12-04T18:57:50Z
2023-12-04T18:57:50Z
2023
Gallego López, M.d.C., Ojeda Murillo, M.L., Romero Herrera, I., Gomes de Figueredo Rua, R.M., Carreras Sánchez, O. y Nogales Bueno, F. (2023). Folic Acid Antioxidant Supplementation to Binge Drinking Adolescent Rats Improves Hydric-saline Balance and Blood Pressure, but Fails to Increase Renal NO Availability and Glomerular Filtration Rate. FASEB Journal, 38 (1), e23341. https://doi.org/10.1096/fj.202301609R.
1530-6860
0892-6638
https://hdl.handle.net/11441/152193
10.1096/fj.202301609R
Binge drinking (BD) is an especially pro-oxidant pattern of alcohol consumption,
particularly widespread in the adolescent population. In the kidneys, it affects the
glomerular filtration rate (GFR), leading to high blood pressure. BD exposure also
disrupts folic acid (FA) homeostasis and its antioxidant properties. The aim of this
study is to test a FA supplementation as an effective therapy against the oxida-
tive, nitrosative, and apoptotic damage as well as the renal function alteration oc-
curred after BD in adolescence. Four groups of adolescent rats were used: control,
BD (exposed to intraperitoneal alcohol), control FA-supplemented group and BD
FA-supplemented group. Dietary FA content in control groups was 2 ppm, and
8 ppm in supplemented groups. BD provoked an oxidative imbalance in the kid-
neys by dysregulating antioxidant enzymes and increasing the enzyme NADPH
oxidase 4 (NOX4), which led to an increase in caspase-9. BD also altered the renal
nitrosative status affecting the expression of the three nitric oxide (NO) synthase
(NOS) isoforms, leading to a decrease in NO levels. Functionally, BD produced a
hydric-electrolytic imbalance, a low GFR and an increase in blood pressure. FA
supplementation to BD adolescent rats improved the oxidative, nitrosative, and
apoptotic balance, recovering the hydric-electrolytic equilibrium and blood pres-
sure. However, neither NO levels nor GFR were recovered, showing in this study for the first time that NO availability in the kidneys plays a crucial role in GFR
regulation that the antioxidant effects of FA cannot repair.
Junta de Andalucía CTS-193
application/pdf
16 p.
eng
Wiley
FASEB Journal, 38 (1), e23341.
CTS-193
https://doi.org/10.1096/fj.202301609R
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Binge drinking
Folic acid
Nitric oxide
Oxidative stress
Renal function
Folic Acid Antioxidant Supplementation to Binge Drinking Adolescent Rats Improves Hydric-saline Balance and Blood Pressure, but Fails to Increase Renal NO Availability and Glomerular Filtration Rate
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Junta de Andalucía
Universidad de Sevilla
FASEB Journal
38
1
e23341
https://ror.org/03yxnpp24
oai:idus.us.es:11441/457932024-02-14T20:12:34Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Tavares Vázquez, Eva
Maldonado Ordóñez, Rosario
Ojeda Murillo, María Luisa
Miñano Sánchez, Javier
2016-09-27T11:24:32Z
2016-09-27T11:24:32Z
2005
Tavares Vázquez, E., Maldonado Ordóñez, R., Ojeda Murillo, M.L. y Miñano Sánchez, J. (2005). Circulating inflammatory mediators during start of fever in differential diagnosis of gram-negative and gram-positive infections in leukopenic rats. Clinical and Diagnostic Laboratory Immunology, 12 (9), 1085-1093.
1071-412X
http://hdl.handle.net/11441/45793
10.1128/CDLI.12.9.1085-1093.2005
https://idus.us.es/xmlui/handle/11441/45793
Gram-negative and gram-positive infections have been considered the most important causes of morbidity
and mortality in patients with leukopenia following chemotherapy. However, discrimination between bacterial
infections and harmless fever episodes is difficult. Because classical inflammatory signs of infection are often
absent and fever is frequently the only sign of infection, the aim of this study was to assess the significance of
serum interleukin-6 (IL-6), IL-10, macrophage inflammatory protein-2 (MIP-2), procalcitonin (PCT), and
C-reactive protein (CRP) patterns in identifying bacterial infections during start of fever in normal and
cyclophosphamide-treated (leukopenic) rats following an injection of lipopolysaccharide (LPS) or muramyl
dipeptide (MDP) as a model for gram-negative and gram-positive bacterial infections. We found that, com-
pared to normal rats, immunosuppressed animals exhibited significantly higher fevers and lesser production
of all mediators, except IL-6, after toxin challenge. Moreover, compared to rats that received MDP, both groups
of animals that received an equivalent dose of LPS showed significantly higher fevers and greater increase in
serum cytokine levels. Furthermore, in contrast to those in immunocompetent rats, serum levels of IL-6 and
MIP-2 were not significantly changed in leukopenic animals after MDP injection. Other serum markers such
as PCT and CRP failed to discriminate between bacterial stimuli in both groups of animals. These results
suggest that the use of the analyzed serum markers at an early stage of fever could give useful information for
the clinician for excluding gram-negative from gram-positive infections
application/pdf
eng
American Society for Microbiology
Clinical and Diagnostic Laboratory Immunology, 12 (9), 1085-1093.
https://dx.doi.org/10.1128/CDLI.12.9.1085-1093.2005
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Circulating inflammatory mediators during start of fever in differential diagnosis of gram-negative and gram-positive infections in leukopenic rats
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Clinical and Diagnostic Laboratory Immunology
12
9
1085
1093
https://ror.org/03yxnpp24
9 p.
oai:idus.us.es:11441/1440722024-02-14T11:26:38Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Koropouli, Eleftheria
Wang, Qiang
Mejías Estévez, Rebeca María
Hand, Randal
Wang, Tao
Ginty, David D.
Kolodkin, Alex L.
2023-04-10T11:37:56Z
2023-04-10T11:37:56Z
2023
Koropouli, E., Wang, Q., Mejías Estévez, R.M., Hand, R., Wang, T., Ginty, D.D. y Kolodkin, A.L. (2023). Palmitoylation regulates neuropilin-2 localization and function in cortical neurons and conveys specificity to semaphorin signaling via palmitoyl acyltransferases.. eLife, 12, e83217. https://doi.org/10.7554/eLife.83217.
2050-084X
https://hdl.handle.net/11441/144072
10.7554/eLife.83217
Secreted semaphorin 3F (Sema3F) and semaphorin 3A (Sema3A) exhibit remarkably
distinct effects on deep layer excitatory cortical pyramidal neurons; Sema3F mediates dendritic
spine pruning, whereas Sema3A promotes the elaboration of basal dendrites. Sema3F and Sema3A
signal through distinct holoreceptors that include neuropilin-2 (Nrp2)/plexinA3 (PlexA3) and neuropilin-1 (Nrp1)/PlexA4, respectively. We find that Nrp2 and Nrp1 are S-palmitoylated in cortical neurons
and that palmitoylation of select Nrp2 cysteines is required for its proper subcellular localization, cell
surface clustering, and also for Sema3F/Nrp2-dependent dendritic spine pruning in cortical neurons,
both in vitro and in vivo. Moreover, we show that the palmitoyl acyltransferase ZDHHC15 is required
for Nrp2 palmitoylation and Sema3F/Nrp2-dependent dendritic spine pruning, but it is dispensable
for Nrp1 palmitoylation and Sema3A/Nrp1-dependent basal dendritic elaboration. Therefore, palmitoyl acyltransferase-substrate specificity is essential for establishing compartmentalized neuronal
structure and functional responses to extrinsic guidance cues.
National Institutes of Health (NIH) de los Estados Unidos-NIH MH100024-Project #3
National Institutes of Health (NIH) de los Estados Unidos-NIH MH100024-Project #3 y NIH R21NS085358
application/pdf
34 p.
eng
eLife Sciences Publications
eLife, 12, e83217.
NIH MH100024-Project #3
NIH R21NS085358
https://doi.org/10.7554/eLife.83217
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Palmitoylation regulates neuropilin-2 localization and function in cortical neurons and conveys specificity to semaphorin signaling via palmitoyl acyltransferases.
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
National Institutes of Health (NIH). EE.UU.
Howard Hughes Medical Institute. EE.UU.
eLife
12
e83217
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1526612024-02-14T20:33:06Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Ojeda Murillo, María Luisa
Sobrino, Paula
Rua, Rui Manuel
Gallego López, María del Carmen
Nogales Bueno, Fátima
Carreras Sánchez, Olimpia
2023-12-18T18:29:28Z
2023-12-18T18:29:28Z
2021-09
Ojeda Murillo, M.L., Sobrino, P., Rua, R.M., Gallego López, M.d.C., Nogales Bueno, F. y Carreras Sánchez, O. (2021). Selenium, a dietary-antioxidant with cardioprotective effects, prevents the impairments in heart rate and systolic blood pressure in adolescent rats exposed to binge drinking treatment. American Journal of Drug and Alcohol Abuse, 47 (6), 680-693. https://doi.org/10.1080/00952990.2021.1973485.
0095-2990
1097-9891
https://hdl.handle.net/11441/152661
10.1080/00952990.2021.1973485
Background: Binge drinking (BD) during adolescence is related to cardiovascular alterations. Selenium (Se) is an essential trace element with antioxidant, anti-inflammatory and antiapoptotic properties, essential for correct heart function. Objectives: To study the protective cardiovascular effects of selenium in adolescent rats exposed to a BD-like procedure. Methods: 32 adolescent male rats exposed to an intraperitoneally BD-like model or not, and supplemented with 0.4ppm of selenite or not, were divided into 4 groups: control, alcohol, control-selenium and alcohol-selenium. Blood pressure and heart rate (HR) were determined after experimentation. Se deposits, oxidative balance and the expression of glutathione peroxidases (GPxs), NF-kB and caspase-3 were measured in the heart. Also, DNA instability in rat lymphocytes and serum vascular markers were determined. Statistical analysis was performed with the ANOVA model. Results: The BD-like model depleted Se heart deposits (p < .01), decreased GPx activity (p < .01) and GPx1 (p < .001) and GPx4 (p < .05) expression, increased NF-kB (p < .01), caspase-3 (p < .001) expression, and generated oxidation in myocytes. Outside the heart, the BD-like model caused double-strand breaks in lymphocyte DNA and increased all the vascular markers measured. These cardiovascular alterations were related to higher systolic (p < .001) and diastolic (p < .05) blood pressure and HR (p < .05). In the heart, Se supplementation in BD-exposed rats significantly increased Se deposits (p < .001) and improved oxidative balance and vascular damage, including increased GPxs and decreased NF-kB and caspase-3 activation, consequently decreasing systolic (p < .05) blood pressure and HR (p < .01). Conclusions: Se supplementation presents cardioprotective effects since it reversed HR and systolic blood pressure observed in BD-exposed adolescent rats.
Junta de Andalucía CTS-193
application/pdf
27 p.
eng
Taylor & Francis
American Journal of Drug and Alcohol Abuse, 47 (6), 680-693.
CTS-193
https://doi.org/10.1080/00952990.2021.1973485
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Binge drinking experience
Heart rate
Selenium
Systolic blood pressure
Vascular markers
Selenium, a dietary-antioxidant with cardioprotective effects, prevents the impairments in heart rate and systolic blood pressure in adolescent rats exposed to binge drinking treatment
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Fisiología
Junta de Andalucía
American Journal of Drug and Alcohol Abuse
47
6
680
693
https://ror.org/03yxnpp24
oai:idus.us.es:11441/962392024-02-13T22:12:42Zcom_11441_10994com_11441_10983com_11441_10690com_11441_10873com_11441_10802col_11441_10995col_11441_10874
Zumárraga Franco, Javier
Argüelles Castilla, Sandro
Ayala Gómez, Antonio
Cano Rodríguez, María Mercedes
2020-05-07T11:11:21Z
2020-05-07T11:11:21Z
2019-10
Zumárraga Franco, J., Argüelles Castilla, S., Ayala Gómez, A. y Cano Rodríguez, M.M. (2019). Physical exercise and myokines. Approaches to Aging Control, 23, 7-16.
2341-5401
2341-5398
https://hdl.handle.net/11441/96239
21867189
Among the types of muscles present in the body
is skeletal muscle, which is the one that allows the
development of physical activity thanks to the contractile
activity of its muscle cells. It is known that
physical exercise involves the release of plasma, by
the skeletal muscle, of molecules called myokines as a
result of muscle contraction. These myokines seem to
be at the base of the beneficial effect of physical exercise
on health. For this reason, this article reviews the
characteristics and properties of the most important
myokines and how they can contribute to a healthier
aging.
application/pdf
10 p.
eng
Spanish Society of Anti-Aging Medicine and Longevity
Approaches to Aging Control, 23, 7-16.
https://www.approachestoagingcontrol.org/archive
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Physical exercise
Skeletal muscle
Myokines
Endocrine organ
Irisin
Physical exercise and myokines
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. Departamento de Bioquímica y Biología Molecular
Approaches to Aging Control
23
7
16
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1477492024-02-17T17:25:01Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Vázquez Carretero, María Dolores
García Miranda, Pablo
Balda, María S.
Matter, Karl
Ilundáin Larrañeta, María Anunciación Ana
Peral Rubio, María José
2023-07-05T14:50:42Z
2023-07-05T14:50:42Z
2021
Vázquez Carretero, M.D., García Miranda, P., Balda, M.S., Matter, K., Ilundáin Larrañeta, M.A.A. y Peral Rubio, M.J. (2021). Proper E-cadherin membrane location in colon requires Dab2 and it modifies by inflammation and cancer. Journal of Cellular Physiology, 236 (2), 1083-1093. https://doi.org/10.1002/jcp.29917.
0021-9541
1097-4652
https://hdl.handle.net/11441/147749
10.1002/jcp.29917
We reported that Disabled-2 (Dab2) is located at the apical membrane in suckling rat intestine. Here, we discovered that, in colon of suckling and adult mouse and of adult human, Dab2 is only at lateral crypt cell membrane and colocalized with E-cadherin. Dab2 depletion in Caco-2 cells led to E-cadherin internalization indicating that its membrane location requires Dab2. In mice, we found that 3 days of dextran sulfate sodium-induced colitis increased Dab2/E-cadherin colocalization, which was decreased as colitis progressed to 6 and 9 days. In agreement, Dab2/E-cadherin colocalization increased in human mild and severe ulcerative colitis and in polyps, being reduced in colon adenocarcinomas, which even showed epithelial Dab2 absence and E-cadherin delocalization. Epithelial Dab2 decrement preceded that of E-cadherin. We suggest that Dab2, by inhibiting E-cadherin internalization, stabilizes adherens junctions, and its absence from the epithelium may contribute to development of colon inflammation and cancer.
Junta de Andalucía CTS 5884
application/pdf
27 p.
eng
Wiley-Blackwell
Journal of Cellular Physiology, 236 (2), 1083-1093.
CTS 5884
https://doi.org/10.1002/jcp.29917
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Cancer
Colon
Dab2
E-cadherin
Inflammation
Junctions
Proper E-cadherin membrane location in colon requires Dab2 and it modifies by inflammation and cancer
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Fisiología
Junta de Andalucía
Journal of Cellular Physiology
236
2
1083
1093
https://ror.org/03yxnpp24
oai:idus.us.es:11441/832582024-02-15T07:51:26Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Escudero González, Miguel
Moreno López, Bernardo
Estrada Cerquera, Carmen
2019-02-20T09:27:17Z
2019-02-20T09:27:17Z
1998
Escudero González, M., Moreno López, B. y Estrada Cerquera, C. (1998). Mechanisms of Action and Targets of Nitric Oxide in the Oculomotor System. Journal of Neuroscience, 18 (24), 10672-10679.
1529-2401
https://hdl.handle.net/11441/83258
10.1523/JNEUROSCI.18-24-10672.1998
Nitric oxide (NO) production by neurons in the prepositus hypoglossi (PH) nucleus is necessary for the normal performance of eye movements in alert animals. In this study, the mechanism(s) of action of NO in the oculomotor system has been investigated. Spontaneous and vestibularly induced eye movements were recorded in alert cats before and after microinjections in the PH nucleus of drugs affecting the NO–cGMP pathway. The cellular sources and targets of NO were also studied by immunohistochemical detection of neuronal NO synthase (NOS) and NO-sensitive guanylyl cyclase, respectively. Injections of NOS inhibitors produced alterations of eye velocity, but not of eye position, for both spontaneous and vestibularly induced eye movements, suggesting that NO produced by PH neurons is involved in the processing of velocity signals but not in the eye position generation. The effect of neuronal NO is probably exerted on a rich cGMP-producing neuropil dorsal to the nitrergic somas in the PH nucleus. On the other hand, local injections of NO donors or 8-Br-cGMP produced alterations of eye velocity during both spontaneous eye movements and vestibulo-ocular reflex (VOR), as well as changes in eye position generation exclusively during spontaneous eye movements. The target of this additional effect of exogenous NO is probably a well defined group of NO-sensitive cGMP-producing neurons located between the PH and the medial vestibular nuclei. These cells could be involved in the generation of eye position signals during spontaneous eye movements but not during the VOR.
Fondo de Investigación Sanitaria Grants 94/0388 and 97/2054
Comunidad Autónoma de Madrid Grant 08.5/0019/1997
Dirección General de Investigación Científica y Technológica Grant PB 93–1175
application/pdf
eng
Society for Neuroscience
Journal of Neuroscience, 18 (24), 10672-10679.
Grants 94/0388 and 97/2054
Grant 08.5/0019/1997
Grant PB 93–1175
http://dx.doi.org/10.1523/JNEUROSCI.18-24-10672.1998
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
eye movements
nitrergic neurons
nitric oxide
oculomotor integrator
prepositus hypoglossi nucleus
soluble guanylyl cyclase
Mechanisms of Action and Targets of Nitric Oxide in the Oculomotor System
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Journal of Neuroscience
18
24
10672
10679
https://ror.org/03yxnpp24
7 p.
oai:idus.us.es:11441/166822024-02-13T09:46:07Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Universidad de Sevilla. Departamento de Fisiología
Ilundáin Larrañeta, María Anunciación Ana
García Miranda, Pablo
Peral Rubio, María José
2014-11-27T12:29:54Z
2014-11-27T12:29:54Z
2010
Ilundáin Larrañeta, M.A., García Miranda, P. y Peral Rubio, M.J. (2010). Effect of antidiuresis on renal creatine metabolism. Journal of Physiology and Pharmacology, 61, 83-88.
0867-5910
http://www.jpp.krakow.pl/journal/archive/02_10/pdf/83_02_10_article.pdf
http://hdl.handle.net/11441/16682
https://idus.us.es/xmlui/handle/11441/16682
eng
Journal of Physiology and Pharmacology, 61, 83-88.
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Effect of antidiuresis on renal creatine metabolism
info:eu-repo/semantics/article
Journal of Physiology and Pharmacology
61
83
88
https://ror.org/03yxnpp24
oai:idus.us.es:11441/653332024-02-14T13:47:21Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Jayabalan, Nanthini
Nair, Soumyalekshmi
Zuñiga, Felipe A.
Sobrevia Luarte, Luis
2017-10-23T16:44:45Z
2017-10-23T16:44:45Z
2017
Jayabalan, N., Nair, ., Zuñiga, F.A. y Sobrevia Luarte, L. (2017). Cross Talk between Adipose Tissue and Placenta in Obese and Gestational Diabetes Mellitus Pregnancies via Exosomes. Frontiers in Endocrinology, 8 (239), 1-18.
1664-2392
http://hdl.handle.net/11441/65333
10.3389/fendo.2017.00239
Obesity is an important public health issue worldwide, where it is commonly associated with the development of metabolic disorders, especially insulin resistance (IR). Maternal obesity is associated with an increased risk of pregnancy complications, especially gestational diabetes mellitus (GDM). Metabolism is a vital process for energy production and the maintenance of essential cellular functions. Excess energy storage is predominantly regulated by the adipose tissue. Primarily made up of adipocytes, adipose tissue acts as the body’s major energy reservoir. The role of adipose tissue, however, is not restricted to a “bag of fat.” The adipose tissue is an endocrine organ, secreting various adipokines, enzymes, growth factors, and hormones that take part in glucose and lipid metabolism. In obesity, the greater portion of the adipose tissue comprises fat, and there is increased pro-inflammatory cytokine secretion, macrophage infiltration, and reduced insulin sensitivity. Obesity contributes to systemic IR and its associated metabolic complications. Similar to adipose tissue, the placenta is also an endocrine organ. During pregnancy, the placenta secretes various molecules to maintain pregnancy physiology. In addition, the placenta plays an important role in metabolism and exchange of nutrients between mother and fetus. Inflammation at the placenta may contribute to the severity of maternal IR and her likelihood of developing GDM and may also mediate the adverse consequences of obesity and GDM on the fetus. Interestingly, studies on maternal insulin sensitivity and secretion of placental hormones have not shown a positive correlation between these phenomena. Recently, a great interest in the field of extracellular vesicles (EVs) has been observed in the literature. EVs are produced by a wide range of cells and are present in all biological fluids. EVs are involved in cell-to-cell communication. Recent evidence points to an association between adipose tissue-derived EVs and metabolic syndrome in obesity. In this review, we will discuss the changes in human placenta and adipose tissue in GDM and obesity and summarize the findings regarding the role of adipose tissue and placenta-derived EVs, with an emphasis on exosomes in obesity, and the contribution of obesity to the development of GDM.
application/pdf
eng
Frontiers Media
Frontiers in Endocrinology, 8 (239), 1-18.
http://dx.doi.org/10.3389/fendo.2017.00239
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
adipose tissue
adipose tissue-derived exosomes
obesity
gestational diabetes
extracellular vesicles
Cross Talk between Adipose Tissue and Placenta in Obese and Gestational Diabetes Mellitus Pregnancies via Exosomes
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Frontiers in Endocrinology
8
239
1
18
https://ror.org/03yxnpp24
19 p.
oai:idus.us.es:11441/940352024-02-14T08:51:44Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Mejías Estévez, Rebeca María
Chiu, Shu-Ling
Rose, Rebecca
Gil Infante, Ana
Zhao, Yifan
Wang, Tao
Huganir, Richard L
2020-03-09T11:46:11Z
2020-03-09T11:46:11Z
2019
Mejías Estévez, R.M., Chiu, S., Rose, R., Gil Infante, A., Zhao, Y., Wang, T. y Huganir, R.L. (2019). Purkinje cell-specific Grip1/2 knockout mice show increased repetitive self-grooming and enhanced mGluR5 signaling in cerebellum. Neurobiology of Disease, 132, 1-10.
1095-953X
https://hdl.handle.net/11441/94035
10.1016/j.nbd.2019.104602
Cerebellar Purkinje cell (PC) loss is a consistent pathological finding in autism. However, neural mechanisms of PC-dysfunction in autism remain poorly characterized. Glutamate receptor interacting proteins 1/2 (Grip1/2) regulate AMPA receptor (AMPAR) trafficking and synaptic strength. To evaluate role of PC-AMPAR signaling in autism, we produced PC-specific Grip1/2 knockout mice by crossing Grip2 conventional and Grip1 conditional KO with L7-Cre driver mice. PCs in the mutant mice showed normal morphology and number, and a lack of Grip1/2 expression. Rodent behavioral testing identified normal ambulation, anxiety, social interaction, and an increase in repetitive self-grooming. Electrophysiology studies revealed normal mEPSCs but an impaired mGluR-LTD at the Parallel Fiber-PC synapses. Immunoblots showed increased expression of mGluR5 and Arc, and enhanced phosphorylation of P38 and AKT in cerebellum of PC-specific Grip1/2 knockout mice. Results indicate that loss of Grip1/2 in PCs contributes to increased repetitive self-grooming, a core autism behavior in mice. Results support a role of AMPAR trafficking defects in PCs and disturbances of mGluR5 signaling in cerebellum in the pathogenesis of repetitive behaviors.
University of Seville (V PPIT-US)
Spain and an National Institute of Health (NIH) (NS085358)
application/pdf
10 p.
eng
Elsevier
Neurobiology of Disease, 132, 1-10.
(V PPIT-US)
NS085358
http://dx.doi.org/10.1016/j.nbd.2019.104602
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
AMPA receptors
Autism
Cerebellum
Glutamate signaling
Grip1/2
Grooming
LTD
Purkinje cells
mGluR receptors
Purkinje cell-specific Grip1/2 knockout mice show increased repetitive self-grooming and enhanced mGluR5 signaling in cerebellum
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla
NIH
Neurobiology of Disease
132
1
10
https://ror.org/03yxnpp24
oai:idus.us.es:11441/641952021-03-12T13:55:04Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874oai:idus.us.es:11441/948312024-02-13T09:50:08Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Carrero Rojas, Génova
Benítez Temiño, Beatriz
Pastor Loro, Ángel Manuel
Davis López de Carrizosa, María América
2020-04-02T11:18:56Z
2020-04-02T11:18:56Z
2020
Carrero Rojas, G., Benítez Temiño, B., Pastor Loro, Á.M. y Davis López de Carrizosa, M.A. (2020). Muscle Progenitors Derived from Extraocular Muscles Express Higher Levels of Neurotrophins and their Receptors than other Cranial and Limb Muscles. Cells, 9 (3), 1-15.
2073-4409
https://hdl.handle.net/11441/94831
10.3390/cells9030747
Extraocular muscles (EOMs) show resistance to muscle dystrophies and sarcopenia. It has been recently demonstrated that they are endowed with different types of myogenic cells, all of which present an outstanding regenerative potential. Neurotrophins are important modulators of myogenic regeneration and act promoting myoblast proliferation, enhancing myogenic fusion rates and protecting myotubes from inflammatory stimuli. Here, we adapted the pre-plate cell isolation technique to obtain myogenic progenitors from the rat EOMs, and quantified their in vitro expression of neurotrophins and their receptors by RT-qPCR and immunohistochemistry, respectively. The results were compared with the expression on progenitors isolated from buccinator, tongue and limb muscles. Our quantitative analysis of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and neurotrophin-3 (NT-3) transcripts showed, for the first time, that EOMs-derived cells express more of these factors and that they expressed TrkA, but not TrkB and TrkC receptors. On the contrary, the immunofluorescence analysis demonstrated high expression of p75NTR on all myogenic progenitors, with the EOMs-derived cells showing higher expression. Taken together, these results suggest that the intrinsic trophic differences between EOMs-derived myogenic progenitors and their counterparts from other muscles could explain why those cells show higher proliferative and fusion rates, as well as better regenerative properties.
España Ministerio de Ciencia, Innovación y Universidades , grant number PGC2018-094654-B-100
application/pdf
15 p.
eng
MDPI
Cells, 9 (3), 1-15.
PGC2018-094654-B-100
https://doi.org/10.3390/cells9030747
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
BDNF
NGF
NT-3
Trk
extraocular muscles
myogenesis
myogenic progenitors
neurotrophins
p75NTR
skeletal muscle
Muscle Progenitors Derived from Extraocular Muscles Express Higher Levels of Neurotrophins and their Receptors than other Cranial and Limb Muscles
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Ciencia, Innovación y Universidades (MICINN). España
Cells
9
3
1
15
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1409492024-02-12T21:57:40Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Dubuisson, Nicolas
Versele, Romain
Planchon, Chloé
Selvais, Camille M.
Noel, Laurence
Davis López de Carrizosa, María América
2023-01-05T09:03:57Z
2023-01-05T09:03:57Z
2022
Dubuisson, N., Versele, R., Planchon, C., Selvais, C.M., Noel, L. y Davis López de Carrizosa, M.A. (2022). Histological Methods to Assess Skeletal Muscle Degeneration and Regeneration in Duchenne Muscular Dystrophy. International Journal of Molecular Sciences, 23 (24), 16080. https://doi.org/10.3390/ijms232416080.
1422-0067
https://hdl.handle.net/11441/140949
10.3390/ijms232416080
Duchenne muscular dystrophy (DMD) is a progressive disease caused by the loss of function of the protein dystrophin. This protein contributes to the stabilisation of striated cells during contraction, as it anchors the cytoskeleton with components of the extracellular matrix through the dystrophin-associated protein complex (DAPC). Moreover, absence of the functional protein affects the expression and function of proteins within the DAPC, leading to molecular events responsible for myofibre damage, muscle weakening, disability and, eventually, premature death. Presently, there is no cure for DMD, but different treatments help manage some of the symptoms. Advances in genetic and exon-skipping therapies are the most promising intervention, the safety and efficiency of which are tested in animal models. In addition to in vivo functional tests, ex vivo molecular evaluation aids assess to what extent the therapy has contributed to the regenerative process. In this regard, the later advances in microscopy and image acquisition systems and the current expansion of antibodies for immunohistological evaluation together with the development of different spectrum fluorescent dyes have made histology a crucial tool. Nevertheless, the complexity of the molecular events that take place in dystrophic muscles, together with the rise of a multitude of markers for each of the phases of the process, makes the histological assessment a challenging task. Therefore, here, we summarise and explain the rationale behind different histological techniques used in the literature to assess degeneration and regeneration in the field of dystrophinopathies, focusing especially on those related to DMD.
application/pdf
38 p.
eng
MDPI
International Journal of Molecular Sciences, 23 (24), 16080.
https://dx.doi.org/10.3390/ijms232416080
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
dystrophin
histology
Duchenne muscular dystrophy
skeletal muscle
myofibre
animal model
regeneration
degeneration
immunofluorescence
immunohistology
Histological Methods to Assess Skeletal Muscle Degeneration and Regeneration in Duchenne Muscular Dystrophy
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
International Journal of Molecular Sciences
23
24
16080
https://ror.org/03yxnpp24
oai:idus.us.es:11441/752972019-04-03T05:54:14Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Fuenzalida, Bárbara
Sobrevia, Bastián
Cantin, Claudette
Sobrevia Luarte, Luis
2018-05-29T08:25:29Z
2018-05-29T08:25:29Z
2018
Fuenzalida, B., Sobrevia, B., Cantin, C. y Sobrevia Luarte, L. (2018). Maternal supraphysiological hypercholesterolemia associates with endothelial dysfunction of the placental microvasculature. Scientific Reports, 8 (7690), 1-10.
2045-2322
https://hdl.handle.net/11441/75297
10.1038/s41598-018-25985-6
Maternal physiological or supraphysiological hypercholesterolemia (MPH, MSPH) occurs during pregnancy. MSPH is associated with foetal endothelial dysfunction and atherosclerosis. However, the potential effects of MSPH on placental microvasculature are unknown. The aim of this study was to determine whether MSPH alters endothelial function in the placental microvasculature both ex vivo in venules and arterioles from the placental villi and in vitro in primary cultures of placental microvascular endothelial cells (hPMEC). Total cholesterol < 280 mg/dL indicated MPH, and total cholesterol ≥280 mg/dL indicated MSPH. The maximal relaxation to histamine, calcitonin gene-related peptide and adenosine was reduced in MSPH venule and arteriole rings. In hPMEC from MSPH placentas, nitric oxide synthase (NOS) activity and L-arginine transport were reduced without changes in arginase activity or the protein levels of endothelial NOS (eNOS), human cationic amino acid 1 (hCAT-1), hCAT-2A/B or arginase II compared with hPMEC from MPH placentas. In addition, it was shown that adenosine acts as a vasodilator of the placental microvasculature and that NOS is active in hPMEC. We conclude that MSPH alters placental microvascular endothelial function via a NOS/L-arginine imbalance. This work also reinforces the concept that placental endothelial cells from the macro- and microvasculature respond differentially to the same pathological condition.
FONDECYT 1150344, 1150377
application/pdf
eng
Nature Publishing Group
Scientific Reports, 8 (7690), 1-10.
1150344
1150377
http://dx.doi.org/ 10.1038/s41598-018-25985-6
Atribución-NoComercial-SinDerivadas 3.0 Estados Unidos de América
Atribución-NoComercial-SinDerivadas 3.0 Estados Unidos de América
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Maternal supraphysiological hypercholesterolemia associates with endothelial dysfunction of the placental microvasculature
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT). Chile
Scientific Reports
8
7690
1
10
10 p.
oai:idus.us.es:11441/475092024-02-13T20:01:51Zcom_11441_10994com_11441_10983com_11441_10690com_11441_10873com_11441_10802col_11441_10995col_11441_10874
Cano Rodríguez, María Mercedes
Muñoz Pinto, Mario Faustino
Ayala Gómez, Antonio
Argüelles Castilla, Sandro
2016-10-14T10:15:37Z
2016-10-14T10:15:37Z
2012
Cano Rodríguez, M.M., Muñoz, M.F., Ayala Gómez, A. y Argüelles, S. (2012). Folic acid supplementation: some practical aspects. Approaches to aging control, Sept. (16), 20-25.
1885-4028
http://hdl.handle.net/11441/47509
https://idus.us.es/xmlui/handle/11441/47509
Since 1956, when Harman first postulated the free radical theory of aging, numerous studies have been carried out to test the protective action of antioxidants. One of these protective compounds used in antioxidant therapy is folic acid (FA). Folate deficiency can lead to several pathologies and its protective role is very well known. Because the negative effects of the synthetic form on the metabolism of folates and the controversy about the role of folic acid in cancer, the question is whether or not folic acid is good for everyone. In this paper we summarize some aspects of the biochemistry of folic acid and we show some precautions that should be taken into consideration when supplementing with this compound.
application/pdf
eng
Federation of Anti-Aging Medicine Societies
Approaches to aging control, Sept. (16), 20-25.
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Folic acid
Folate
Antioxidant
Qxidative stress
Homocysteine
Folic acid supplementation: some practical aspects
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. Departamento de Bioquímica y Biología Molecular
Approaches to aging control
Sept.
16
20
25
https://ror.org/03yxnpp24
6 p.
oai:idus.us.es:11441/676332024-02-13T22:15:44Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Sobrevia Luarte, Luis
Myatt, Leslie
Rice, Gregory
2017-12-14T19:03:50Z
2017-12-14T19:03:50Z
2014
Sobrevia Luarte, L., Myatt, . y Rice, G. (2014). Diseases of Pregnancy and Fetal Programming: Cell and Molecular Mechanisms. BioMed Research International, 937050, 1-3.
2314-6133
http://hdl.handle.net/11441/67633
10.1155/2014/937050
application/pdf
eng
Hindawi Publishing Corporation
BioMed Research International, 937050, 1-3.
10.1155/2014/937050
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Diseases of Pregnancy and Fetal Programming: Cell and Molecular Mechanisms
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
BioMed Research International
937050
1
3
https://ror.org/03yxnpp24
3 p.
oai:idus.us.es:11441/1554372024-02-22T13:18:52Zcom_11441_10989com_11441_10983com_11441_10690com_11441_10692com_11441_10691com_11441_11190com_11441_11099com_11441_10873com_11441_10802com_11441_11366com_11441_11290col_11441_10990col_11441_10693col_11441_43346col_11441_10874col_11441_11367
Villar Angulo, Luis Miguel
Correa-Manfredi, Juliana
Gaytán Guía, Susana Pilar
Hervás Gómez, Carlos
Maldonado y Aibar, María Dolores
Medianero-Hernández, José Mª
Ortega-Rodas, Amalia
Reyes, Miguel Mª
Romero Tena, Rosalía
Cantillana Merchante, Concepción
2024-02-21T15:43:38Z
2024-02-21T15:43:38Z
2003
Villar Angulo, L.M., Correa-Manfredi, J., Gaytán Guía, S.P., Hervás Gómez, C., Maldonado y Aibar, M.D., Medianero-Hernández, J.M.,...,Cantillana Merchante, C. (2003). Innovación curricular, ambiente percibido y desarrollo profesional en la Universidad de Sevilla. Revista española de pedagogía, 225, 265-283.
0034-9461
https://hdl.handle.net/11441/155437
Este artículo reúne, primero, las percepciones heterogéneas que tuvieron estudiantes de la Universidad de Sevilla
sobre la comunicación didáctica en diez
aulas mientras se desarrollaban innovaciones curriculares, que no tuvieron un
tiempo único de implantación: el emplazamiento de las mismas se dilató a lo
largo del curso 2000-2001 en materias
anuales y cuatrimestrales. Segundo, el
profesorado utilizó registros metodológicos distintos, una mezcla de acordes
innovadores de donde brotó una rica variedad estilística
application/pdf
19 p.
spa
UNIV INT LA RIOJA-UNIR
Revista española de pedagogía, 225, 265-283.
https://dialnet.unirioja.es/servlet/articulo?codigo=719063
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Innovación curricular, ambiente percibido y desarrollo profesional en la Universidad de Sevilla
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular
Universidad de Sevilla. Departamento de Didáctica y Organización Educativa
Universidad de Sevilla. Departamento de Dibujo
Universidad de Sevilla. Departamento de Ingeniería Gráfica
Revista española de pedagogía
225
265
283
oai:idus.us.es:11441/1480822024-02-15T07:38:11Zcom_11441_10989com_11441_10983com_11441_10690com_11441_10994com_11441_10873com_11441_10802col_11441_10990col_11441_10995col_11441_10874
Real, Luis Miguel
Sáez, María E.
Corma Gómez, Anaïs
González Pérez, Antonio
Thorball, Christian
Ruiz Laza, Rocío
Jiménez León, María Reyes
González-Serna Martín, Manuel Alejandro
Bachiller, Sara
Ruiz Mateos, Ezequiel
2023-07-19T10:25:15Z
2023-07-19T10:25:15Z
2023
Real, L.M., Sáez, M.E., Corma Gómez, A., González Pérez, A., Thorball, C., Ruiz Laza, R.,...,Ruiz Mateos, E. (2023). A metagenome-wide association study of HIV disease progression in HIV controllers. iScience, 26 (7), 107214. https://doi.org/10.1016/j.isci.2023.107214.
2589-0042
https://hdl.handle.net/11441/148082
10.1016/j.isci.2023.107214
Some HIV controllers experience immunologic progression with CD4+ T cell decline. We aimed to identify genetic factors associated with CD4+ T cell lost in HIV controllers. A total of 561 HIV controllers were included, 442 and 119 from the International HIV controllers Study Cohort and the Swiss HIV Cohort Study, respectively. No SNP or gene was associated with the long-term non-progressor HIV spontaneous control phenotype in the individual GWAS or in the meta-analysis. However, SNPs previously associated with natural HIV control linked to HLA-B (rs2395029 [p = 0.005; OR = 1.70], rs59440261 [p = 0.003; OR = 1.78]), MICA (rs112243036 [p = 0.011; OR = 1.45]), and PSORS1C1 loci (rs3815087 [p = 0.017; OR = 1.39]) showed nominal association with this phenotype. Genetic factors associated with the long-term HIV controllers without risk of immunologic progression are those previously related to the overall HIV controller phenotype.
Junta de Andalucía (PI-0001/2017)
Plan Nacional de I + D + I cofinanced by ISCIII-Subdirección General de Evaluación and Fondo Europeo de Desarrollo Regional (FEDER) co-funded by the European Union (RD16/0025/ 0040, RD12/0017/0012)
Instituto de Salud Carlos III, Fondos Europeos para el Desarrollo Regional, FEDER, co-funded by the European Union grants PI16/00684, PI19/01127, PI22/ 01796, PI10/02635, PI13/0796, PI16/00503 and PI19/01337
RETICS, Red de Investigación en SIDA co-funded by the European Union (RD16/0025/0020, RD16/0025/0006-ISCIII-FEDER)
application/pdf
12 p.
eng
Elsevier
iScience, 26 (7), 107214.
PI-0001/2017
RD16/0025/0040
RD12/0017/0012
PI16/00684
PI19/01127
PI22/01796
PI10/02635
PI13/0796
PI16/00503
PI19/01337
RD16/0025/0020
RD16/0025/0006
https://dx.doi.org/10.1016/j.isci.2023.107214
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
A metagenome-wide association study of HIV disease progression in HIV controllers
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Bioquímica y Biología Molecular
Universidad de Sevilla. Departamento de Bioquímica Médica y Biología Molecular e Inmunología
Universidad de Sevilla. Departamento de Fisiología
Junta de Andalucía
European Union (UE)
European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
Instituto de Salud Carlos III
Agencia Estatal de Investigación. España
iScience
26
7
107214
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1382452024-02-14T08:59:00Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Reissig, L. F.
Carrero Rojas, Génova
Maierhofer, U.
Moghaddam, A. S.
Hainfellner, A.
Gesslbauer, Bernhard
Haider, T.
Streicher, Johannes
Aszmann, O. C.
Pastor Loro, Ángel Manuel
Weninger, W. J.
Blumer, Roland
2022-10-21T18:11:57Z
2022-10-21T18:11:57Z
2022
Reissig, L.F., Carrero Rojas, G., Maierhofer, U., Moghaddam, A.S., Hainfellner, A., Gesslbauer, B.,...,Blumer, R. (2022). Spinal cord from body donors is suitable for multicolor immunofluorescence. Histochemistry and Cell Biology. https://doi.org/10.1007/s00418-022-02154-5.
0948-6143
1432-119X
https://hdl.handle.net/11441/138245
10.1007/s00418-022-02154-5
Immunohistochemistry is a powerful tool for studying neuronal tissue from humans at the molecular level. Obtaining fresh neuronal tissue from human organ donors is difficult and sometimes impossible. In anatomical body donations, neuronal tissue is dedicated to research purposes and because of its easier availability, it may be an alternative source for research. In this study, we harvested spinal cord from a single organ donor 2 h (h) postmortem and spinal cord from body donors 24, 48, and 72 h postmortem and tested how long after death, valid multi-color immunofluorescence or horseradish peroxidase (HRP) immunohistochemistry is possible. We used general and specific neuronal markers and glial markers for immunolabeling experiments. Here we showed that it is possible to visualize molecularly different neuronal elements with high precision in the body donor spinal cord 24 h postmortem and the quality of the image data was comparable to those from the fresh organ donor spinal cord. High-contrast multicolor images of the 24-h spinal cords allowed accurate automated quantification of different neuronal elements in the same sample. Although there was antibody-specific signal reduction over postmortem intervals, the signal quality for most antibodies was acceptable at 48 h but no longer at 72 h postmortem. In conclusion, our study has defined a postmortem time window of more than 24 h during which valid immunohistochemical information can be obtained from the body donor spinal cord. Due to the easier availability, neuronal tissue from body donors is an alternative source for basic and clinical research.
application/pdf
23 p.
eng
Springer Nature
Histochemistry and Cell Biology.
https://doi.org/10.1007/s00418-022-02154-5
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Horseradish peroxidase immunohistochemistry
Human spinal cord
Multicolor immunofluorescence
Neuronal markers
Postmortem interval
Spinal cord from body donors is suitable for multicolor immunofluorescence
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Histochemistry and Cell Biology
https://ror.org/03yxnpp24
oai:idus.us.es:11441/853352024-02-13T08:46:07Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Carvajal Vázquez, Ana Eloisa
Serrano Morales, José Manuel
Vázquez Carretero, María Dolores
García Miranda, Pablo
Calonge Castrillo, María Luisa
Peral Rubio, María José
Ilundáin Larrañeta, María Anunciación Ana
2019-04-08T11:27:49Z
2019-04-08T11:27:49Z
2017
Carvajal Vázquez, A.E., Serrano Morales, J.M., Vázquez Carretero, M.D., García Miranda, P., Calonge Castrillo, M.L., Peral Rubio, M.J. y Ilundáin Larrañeta, M.A.A. (2017). Reelin protects from colon pathology by maintaining the intestinal barrier integrity and repressing tumorigenic genes. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1863 (9), 2126-2134.
1388-1981
https://hdl.handle.net/11441/85335
10.1016/j.bbadis.2017.05.026
We previously reported that reelin, an extracellular matrix protein first known for its key role in neuronal migration, reduces the susceptibility to dextran sulphate sodium (DSS)-colitis. The aim of the current study was to determine whether reelin protects from colorectal cancer and how reelin defends from colon pathology. In the colon of wild-type and of mice lacking reelin (reeler mice) we have analysed the: i) epithelium cell renewal processes, ii) morphology, iii) Sox9, Cdx2, Smad5, Cyclin D1, IL-6 and IFNγ mRNA abundance in DSS-treated and untreated mice, and iv) development of azoxymethane/DSS-induced colorectal cancer, using histological and real time-PCR methodologies. The reeler mutation increases colitis-associated tumorigenesis, with increased tumours number and size. It also impairs the intestinal barrier because it reduces cell proliferation, migration, differentiation and apoptosis; decreases the number and maturation of goblet cells, and expands the intercellular space of the desmosomes. The intestinal barrier impairment might explain the increased susceptibility to colon pathology exhibited by the reeler mice and is at least mediated by the down-regulation of Sox9 and Cdx2. In response to DSS-colitis, the reeler colon increases the mRNA abundance of IL-6, Smad5 and Cyclin D1 and decreases that of IFNγ, conditions that might result in the increased colitis-associated tumorigenesis found in the reeler mice. In conclusion, the results highlight a role for reelin in maintaining intestinal epithelial cell homeostasis and providing resistance against colon pathology.
España, Junta de Andalucía CTS 5884
application/pdf
eng
Elsevier
Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1863 (9), 2126-2134.
CTS 5884
http://dx.doi.org/10.1016/j.bbadis.2017.05.026
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/acceptedVersion
Cancer
Colitis
Reelin
Reelin protects from colon pathology by maintaining the intestinal barrier integrity and repressing tumorigenic genes
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Fisiología
Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids
1863
9
2126
2134
https://ror.org/03yxnpp24
9 p.
oai:idus.us.es:11441/573672017-05-19T08:47:01Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874oai:idus.us.es:11441/562542017-05-22T12:55:35Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Navarro, Francisco
Forjan Lozano, Eduardo
Vazquez, María
Montero, Zaida
Bermejo, Elisabeth
Pásaro Dionisio, María Rosario
Vega Piqueres, José María
2017-03-24T14:30:25Z
2017-03-24T14:30:25Z
2016
Navarro, F., Forjan Lozano, E., Vazquez, M., Montero, Z., Bermejo, E., Pásaro Dionisio, M.R. y Vega Piqueres, J.M. (2016). Microalgae as a safe food source for animals: nutritional characteristics of the acidophilic microalga Coccomyxa onubensis. Food and Nutrition Research, 60
1654-6628
http://hdl.handle.net/11441/56254
10.3402/fnr.v60.30472
Background
Edible microalgae are marine or fresh water mesophilic species. Although the harvesting of microalgae offers an abundance of opportunities to the food and pharmaceutical industries, the possibility to use extremophilic microalgae as a food source for animals is not well-documented.
Objective
We studied the effects of dietary supplementation of a powdered form of the acidophilic microalga Coccomyxa onubensis on growth and health parameters of laboratory rats.
Method
Four randomly organized groups of rats (n=6) were fed a standard diet (Diet 1, control) or with a diet in which 0.4% (Diet 2), 1.25% (Diet 3), or 6.25% (Diet 4) (w/w) of the standard diet weight was substituted with dried microalgae powder, respectively. The four groups of animals were provided ad libitum access to feed for 45 days.
Results
C. onubensis biomass is rich in protein (44.60% of dry weight) and dietary fiber (15.73%), and has a moderate carbohydrate content (24.8%) and a low lipid content (5.4%) in which polyunsaturated fatty acids represent 65% of the total fatty acid. Nucleic acids are present at 4.8%. No significant difference was found in growth rates or feed efficiency ratios of the four groups of rats. Histological studies of liver and kidney tissue revealed healthy organs in control and C. onubensis-fed animals, while plasma hematological and biochemical parameters were within healthy ranges for all animals. Furthermore, animals fed a microalgae-enriched diet exhibited a statistically significant decrease in both blood cholesterol and triglyceride levels. The blood triglyceride content and very low density lipoprotein-cholesterol levels decreased by about 50% in rats fed Diet 4.
Conclusions
These data suggest that C. onubensis may be useful as a food supplement for laboratory animals and may also serve as a nutraceutical in functional foods. In addition, microalgae powder-supplemented diets exerted a significant hypocholesterolemic and hypotriglyceridemic effect in animals.
application/pdf
eng
Taylor & Francis Open
Food and Nutrition Research, 60
http://dx.doi.org/10.3402/fnr.v60.30472
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Coccomyxa onubensis
safe food
hypolipidemic induction
nutraceuticals
Microalgae as a safe food source for animals: nutritional characteristics of the acidophilic microalga Coccomyxa onubensis
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Food and Nutrition Research
60
oai:idus.us.es:11441/325282024-02-14T20:18:26Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Ilundáin Larrañeta, María Anunciación Ana
Lluch, M.
Ponz, F.
2016-01-14T08:26:47Z
2016-01-14T08:26:47Z
1979
0034-9402
http://hdl.handle.net/11441/32528
https://idus.us.es/xmlui/handle/11441/32528
Sugar absorption by the small intestine has been studied in rat and hamster in vivo, with luminal perfusion, during 1 minute successive periods. Transport is calculated as the difference between absorption and diffusion. The diffusion component is evaluated in the presence of phlorizin or as absorption of sorbose. The resulting KT values for glucose and galactose (rat: 7.7 and 10 mM; hamster: 10 and 14 mM) and 3-0-methyl-glucose (hamster: 25-33 mM) are quite lower than those previously obtained in vivo, but still higher than those in vitro.
The physiological levels of glucose in the intestine of normally fed animals imply that the diffusion component plays an important role in the proximal regions of the small intestine, especially in rat.
application/pdf
eng
Revista Española de Fisiología, 35, 359-366
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Kinetics of Intestinal Sugar transport, in vivo
info:eu-repo/semantics/article
info:eu-repo/semantics/submittedVersion
Universidad de Sevilla. Departamento de Fisiología
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1464662024-02-14T19:17:58Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Carrero Rojas, Génova
Martín Calvo, Paula
Lischka, Thomas
Streicher, Johannes
Rodríguez de la Cruz, Rosa María
Pastor Loro, Ángel Manuel
Blumer, Roland
2023-05-19T14:59:41Z
2023-05-19T14:59:41Z
2022
Carrero Rojas, G., Martín Calvo, P., Lischka, T., Streicher, J., Rodríguez de la Cruz, R.M., Pastor Loro, Á.M. y Blumer, R. (2022). Eye Movements But Not Vision Drive the Development of Palisade Endings. Investigative Ophthalmology and Visual Science, 63 (11). https://doi.org/10.1167/iovs.63.11.15.
0146-0404
1552-5783
https://hdl.handle.net/11441/146466
10.1167/iovs.63.11.15
PURPOSE. To test whether visual experience and/or eye movements drive the postnatal development of palisade endings in extraocular muscles. METHODS. In three newborn cats, the right eye was covered until 30 days from postnatal (P) day 7 (before opening their eyes), and in three cats both eyes were covered until 45 days, also from P7. To block eye movements, another seven cats received a retrobulbar injection of botulinum neurotoxin A (BoNT-A) into the left orbit at birth and survived for 45 days (three cats) and 95 days (four cats). The distal third of the rectus muscles containing the palisade endings was used for whole-mount preparation and triple-fluorescence labeling with anti-neurofilament along with (1) anti-synaptophysin and phalloidin or (2) anti-growth associated protein 43 (GAP43) and phalloidin. Immunolabeled specimens were analyzed in the confocal laser scanning microscope. RESULTS. After unilateral and bilateral dark rearing, palisade endings were qualitatively and quantitatively equal to those from age-matched controls. After BoNT-A induced eye immobilization for 45 or 95 days, palisade endings were absent in the superior rectus and lateral rectus muscles and only present in the inferior rectus and medial rectus muscle. These BoNT-A–treated palisade endings were rudimentary and reduced in number, and the expression of the neuronal developmental protein GAP43 was significantly reduced. CONCLUSIONS. This study demonstrates that eye immobilization, but not visual deprivation, affects palisade ending development. Palisade endings develop in the first month of life, and the present findings indicate that, during this time window, palisade endings are prone to oculomotor perturbations.
Austrian Science Fund P32463- B
Ministerio de Ciencia e Innovación PGC2018-094654- B-100, PID2021-124300NB-I00
Junta de Andalucía P20_00529
application/pdf
18 p.
eng
Association for Research in Vision and Ophthalmology
Investigative Ophthalmology and Visual Science, 63 (11).
P32463- B
PGC2018-094654- B-100
PID2021-124300NB-I00
P20_00529
https://doi.org/10.1167/iovs.63.11.15
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Botulinum neurotoxin
Eye muscles
Palisade endings
Proprioception
Eye Movements But Not Vision Drive the Development of Palisade Endings
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Austrian Science Fund
Ministerio de Ciencia e Innovación (MICIN). España
Junta de Andalucía
Investigative Ophthalmology and Visual Science
63
11
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1488402024-02-14T11:06:04Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Dubuisson, Nicolas
Versele, Romain
Davis López de Carrizosa, María América
Selvais, Camille M.
Noel, Laurence
Planchon, Chloe
Van den Bergh, Peter Y. K.
Brichard, Sonia M.
Abou-Samra, Michel
2023-09-08T13:36:50Z
2023-09-08T13:36:50Z
2023
Dubuisson, N., Versele, R., Davis López de Carrizosa, M.A., Selvais, C.M., Noel, L., Planchon, C.,...,Abou-Samra, M. (2023). The Adiponectin Receptor Agonist, ALY688: A Promising Therapeutic for Fibrosis in the Dystrophic Muscle. Cells, 12 (16), 2101. https://doi.org/10.3390/cells12162101.
2073-4409
https://hdl.handle.net/11441/148840
10.3390/cells12162101
Duchenne muscular dystrophy (DMD) is one of the most devastating myopathies, where severe inflammation exacerbates disease progression. Previously, we demonstrated that adiponectin (ApN), a hormone with powerful pleiotropic effects, can efficiently improve the dystrophic phenotype. However, its practical therapeutic application is limited. In this study, we investigated ALY688, a small peptide ApN receptor agonist, as a potential novel treatment for DMD. Four-week-old mdx mice were subcutaneously treated for two months with ALY688 and then compared to untreated mdx and wild-type mice. In vivo and ex vivo tests were performed to assess muscle function and pathophysiology. Additionally, in vitro tests were conducted on human DMD myotubes. Our results showed that ALY688 significantly improved the physical performance of mice and exerted potent anti-inflammatory, anti-oxidative and anti-fibrotic actions on the dystrophic muscle. Additionally, ALY688 hampered myonecrosis, partly mediated by necroptosis, and enhanced the myogenic program. Some of these effects were also recapitulated in human DMD myotubes. ALY688’s protective and beneficial properties were mainly mediated by the AMPK-PGC-1α axis, which led to suppression of NF-κβ and TGF-β. Our results demonstrate that an ApN mimic may be a promising and effective therapeutic prospect for a better management of DMD.
Fund for Scientific Research—FNRS PDR/T.0026.21
application/pdf
23 p.
eng
Multidisciplinary Digital Publishing Institute (MDPI)
Cells, 12 (16), 2101.
PDR/T.0026.21
https://doi.org/10.3390/cells12162101
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Adiponectin
ALY688
AMPK
Duchenne muscular dystrophy
Fibrosis
Inflammation
Myonecrosis
Necroptosis
Regeneration
Skeletal muscle
The Adiponectin Receptor Agonist, ALY688: A Promising Therapeutic for Fibrosis in the Dystrophic Muscle
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Fund for Scientific Research. Belgium
Cells
12
16
2101
https://ror.org/03yxnpp24
oai:idus.us.es:11441/453912018-04-06T10:37:54Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874oai:idus.us.es:11441/662682024-02-14T09:11:22Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Silva Hucha, Silvia
Benítez Temiño, Beatriz
Pastor Loro, Ángel Manuel
Rodríguez de la Cruz, Rosa María
Morcuende Fernández, Sara R.
García Hernández, Rosendo Miguel
2017-11-20T16:39:26Z
2017-11-20T16:39:26Z
2017
Silva Hucha, S., Benítez Temiño, B., Pastor, Á.M., Rodríguez de la Cruz, R.M., Morcuende Fernández, S.R. y García Hernández, R.M. (2017). Extraocular motoneurons of the adult rat show higher levels of vascular endothelial growth factor and its receptor Flk-1 than other cranial motoneurons. PLoS One, 12 (6), 1-17.
1932-6203
http://hdl.handle.net/11441/66268
10.1371/journal.pone.0178616
Recent studies show a relationship between the deficit of vascular endothelial growth factor (VEGF) and motoneuronal degeneration, such as that occurring in amyotrophic lateral sclerosis (ALS). VEGF delivery protects motoneurons from cell death and delayed neurodegeneration in animal models of ALS. Strikingly, extraocular motoneurons show lesser vulnerability to neurodegeneration in ALS compared to other cranial or spinal motoneurons. Therefore, the present study investigates possible differences in VEGF and its main receptor VEGFR-2 or Flk-1 between extraocular and non-extraocular brainstem motoneurons. We performed immunohistochemistry and Western blot to determine the presence of VEGF and Flk-1 in rat motoneurons located in the three extraocular motor nuclei (abducens, trochlear and oculomotor) and to compare it to that observed in two other brainstem nuclei (hypoglossal and facial) that are vulnerable to degeneration. Extraocular motoneurons presented higher amounts of VEGF and its receptor Flk-1 than other brainstem motoneurons, and thus these molecules could be participating in their higher resistance to neurodegeneration. In conclusion, we hypothesize that differences in VEGF availability and signaling could be a contributing factor to the different susceptibility of extraocular motoneurons, when compared with other motoneurons, in neurodegenerative diseases.
application/pdf
eng
Public Library of Science
PLoS One, 12 (6), 1-17.
http://dx.doi.org/10.1371/journal.pone.0178616
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Extraocular motoneurons of the adult rat show higher levels of vascular endothelial growth factor and its receptor Flk-1 than other cranial motoneurons
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
PLoS One
12
6
1
17
https://ror.org/03yxnpp24
18 p.
oai:idus.us.es:11441/287772024-02-13T20:17:18Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Blaya, J. A.
Muriana, Francisco J. G.
Ruiz Gutiérrez, Valentina
Vázquez Cueto, Carmen María
Bolufer González, José
2015-09-23T12:10:11Z
2015-09-23T12:10:11Z
1998
0144-8463
http://hdl.handle.net/11441/28777
http://dx.doi.org/10.1023/A:1022236616277
https://idus.us.es/xmlui/handle/11441/28777
The transport system of folic acid (Pte-Glu) b y brush-borde r membrane vesicles (BBMV ) isolated from prawn (Penaeus japonicm) hepatopancreas , was studied by measuring the uptake of Pte-Glu . This uptake was found to have two components , intravesicular transport and membrane binding . Membrane binding was not affected by the presence of a trans - membrane pH-gradient at a short incubation period . However , a transmembrane pH - gradient increased membrane binding at 6 0 min. The transpor to f Pte-Glu appeared to be carrier-mediated , was stimulated by an inwardly proton gradient (p H 5. 5 outside , 7. 4 inside ) and was unaffected by a sodium-gradient . The relationship between pH gradient-driven Pte-Glu uptake and medium Pte-Glu concentration followed saturating Michaelis-Menten kinetics . Eadie-Hofste e representation of the pH gradient-driven Pte-Glu uptake indicated a single transport system with a Km of 0.3 7 ^ Man d Vmax of 1.06pmol/mg protein/15s . These findings indicate that BBM V isolated from prawn hepatopancreas possesses a Pte - Glu transport system similar to that described in mammalian intestine.
application/pdf
eng
Portland Press
Bioscience Reports, 18(1), 9-17
http://dx.doi.org/10.1023/A:1022236616277
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Folate transport
prawn
hepatopancreas
brush-border membrane vesicles
kinetics
Folate transport by prawn hepatopancreas brush-border membrane vesicles.
info:eu-repo/semantics/article
Universidad de Sevilla. Departamento de Fisiología
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1341712024-02-14T11:14:05Zcom_11441_11024com_11441_10983com_11441_10690com_11441_10873com_11441_10802col_11441_11025col_11441_10874
Macías Sánchez, Juan
González Moreno, Paz
Sánchez García, Esther
Morillo Verdugo, Ramón Alejandro
Pérez Venegas, José J.
Pinilla, Ana
Macho, M. Mar
Martínez, M. Victoria
González-Serna Martín, Manuel Alejandro
Corma, Anaïs
Real, Luis M.
Pineda Vergara, Juan Antonio
2022-06-07T16:01:21Z
2022-06-07T16:01:21Z
2021
Macías Sánchez, J., González Moreno, P., Sánchez García, E., Morillo Verdugo, R.A., Pérez Venegas, J.J., Pinilla, A.,...,Pineda Vergara, J.A. (2021). Similar incidence of coronavirus disease 2019 (COVID-19) in patients with rheumatic diseases with and without hydroxychloroquine therapy. PLoS ONE, 16 (4), e0249036.
1932-6203
https://hdl.handle.net/11441/134171
10.1371/journal.pone.0249036
Background Hydroxychloroquine is not efficacious as post-exposure prophylaxis against coronavirus disease 2019 (COVID-19). It is not known whether as pre-exposure prophylaxis it may prevent COVID-19. Objective To compare the incidence of COVID-19 in Spanish patients with autoimmune rheumatic diseases treated with and without hydroxychloroquine. Patients and methods Retrospective electronic record review, from February 27th to June 21st, 2020, of patients with autoimmune inflammatory diseases followed at two academic tertiary care hospitals in Seville, Spain. The cumulative incidence of confirmed COVID-19, by PCR or serology, was compared between patients with and without hydroxychloroquine as part of their treatment of autoimmune inflammatory diseases. Results Among 722 included patients, 290 (40%) were receiving hydroxychloroquine. During the seventeen-week study period, 10 (3.4% [95% CI: 1.7%-6.7%] cases of COVID-19 were registered among patients with hydroxychloroquine and 13 (3.0% [1.6%-5.1%]) (p = 0.565) in those without hydroxychloroquine. COVID-19 was diagnosed by PCR in four (1.4%, 95% CI 0.38%-3.5%) subject with hydroxychloroquine and six (1.4%, 95% CI 0.5%-3.0%) without hydroxychloroquine (p = 0.697). Three patients on hydroxychloroquine and four patients without hydroxychloroquine were admitted to the hospital, none of them required to be transferred to the intensive care unit and no patient died during the episode. Conclusions The incidence and severity of COVID-19 among patients with autoimmune rheumatic diseases with and without hydroxychloroquine was not significantly different.
Instituto de Salud Carlos III I3SNS
Ministerio de Ciencia, Innovación y Universidades CP18/00146
application/pdf
6 p.
eng
Public Library of Science
PLoS ONE, 16 (4), e0249036.
I3SNS
CP18/00146
https://doi.org/10.1371/journal.pone.0249036
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Similar incidence of coronavirus disease 2019 (COVID-19) in patients with rheumatic diseases with and without hydroxychloroquine therapy
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Farmacología
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. Departamento de Medicina
Instituto de Salud Carlos III
Ministerio de Ciencia, Innovación y Universidades (MICINN). España
PLoS ONE
16
4
e0249036
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1010822024-02-17T17:54:29Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Reyes Goya, Claudia
Santana Garrido, Álvaro
Soto Astacio, Estefanía
Aramburu Bodas, Oscar
Zambrano, Sonia
Mate Barrero, Alfonso
Vázquez Cueto, Carmen María
2020-09-14T14:18:50Z
2020-09-14T14:18:50Z
2020
Reyes Goya, C., Santana Garrido, Á., Soto Astacio, E., Aramburu Bodas, O., Zambrano, S., Mate Barrero, A. y Vázquez Cueto, C.M. (2020). Mechanism of vascular toxicity in rats subjected to treatment with a tyrosine kinase inhibitor. Toxics, 8 (3), 49-.
2305-6304
https://hdl.handle.net/11441/101082
10.3390/toxics8030049
Sunitinib (Su) is a tyrosine kinase inhibitor with antiangiogenic and antineoplastic effects that is recommended therapy for renal cell carcinoma, gastrointestinal stromal tumors, and pancreatic neuroendocrine tumors. Arterial hypertension is one of the adverse effects observed in the treatment with Su. The aim of this work was to deepen our understanding of the underlying mechanisms involved in the development of this side effect. Studies on endothelial function, vascular remodeling and nicotinamide adenine dinucleotide phosphate oxidase (NADPH oxidase) system were carried out in thoracic aortas from rats treated with Su for three weeks. Animals subjected to Su treatment presented with increased blood pressure and reduced endothelium-dependent vasodilation, the latter being reverted by NADPH oxidase blockade. Furthermore, vascular remodeling and stronger Masson trichrome staining, together with enhanced immunofluorescence signal for collagen 1 alpha 1 (Col1ff1), were observed in aortas from treated animals. These results were accompanied by a significant elevation in superoxide anion production and the activity/protein/gene expression of NADPH oxidase isoforms (NOX1, NOX2, and NOX4), which was also prevented by NOX inhibition. Furthermore, a decrease in nitric oxide (NO) levels and endothelial nitric oxide synthase (eNOS) activation was observed in aortas from Su-treated animals. All these results indicate that endothelial dysfunction secondary to changes in vascular remodeling and oxidative stress might be responsible for the typical arterial hypertension that develops following treatment with Su.
Junta de Andalucía 2017/440
application/pdf
17 p.
eng
Multidisciplinary Digital Publishing Institute (MDPI)
Toxics, 8 (3), 49-.
2017/440
https://doi.org/10.3390/toxics8030049
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Arterial hypertension
Endothelial dysfunction
Tyrosine kinase inhibitor
Vascular remodeling
Mechanism of vascular toxicity in rats subjected to treatment with a tyrosine kinase inhibitor
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Toxics
8
3
49
https://ror.org/03yxnpp24
oai:idus.us.es:11441/872802024-02-17T16:59:59Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Vázquez Carretero, María Dolores
García Miranda, Pablo
Balda, María S.
Matter, Karl
Peral Rubio, María José
Ilundáin Larrañeta, María Anunciación Ana
2019-06-07T16:52:22Z
2019-06-07T16:52:22Z
2018
Vázquez Carretero, M.D., García Miranda, P., Balda, M.S., Matter, K., Peral Rubio, M.J. y Ilundáin Larrañeta, M.A.A. (2018). Small and large intestine express a truncated Dab1 isoform that assembles in cell-cell junctions and co-localizes with proteins involved in endocytosis. Biochimica et Biophysica Acta - Biomembranes, 1860 (5), 1231-1241.
0005-2736
1879-2642
https://hdl.handle.net/11441/87280
10.1016/j.bbamem.2018.02.014
Disabled-1 (Dab1) is an essential intracellular adaptor protein in the reelin pathway. Our previous studies in mice intestine showed that Dab1 transmits the reelin signal to cytosolic signalling pathways. Here, we determine the Dab1 isoform expressed in rodent small and large intestine, its subcellular location and co-localization with clathrin, caveolin-1 and N-Wasp. PCR and sequencing analysis reveal that rodent small and large intestine express a Dab1 isoform that misses three (Y198, Y200 and Y220) of the five tyrosine phosphorylation sites present in brain Dab1 isoform (canonical) and contains nuclear localization and export signals. Western blot assays show that both, crypts, which shelter progenitor cells, and enterocytes express the same Dab1 isoform, suggesting that epithelial cell differentiation does not regulate intestinal generation of alternatively spliced Dab1 variants. They also reveal that the canonical and the intestinal Dab1 isoforms differ in their total degree of phosphorylation. Immunostaining assays show that in enterocytes Dab1 localizes at the apical and lateral membranes, apical vesicles, close to adherens junctions and desmosomes, as well as in the nucleus; co-localizes with clathrin and with N-Wasp but not with caveolin-1, and in Caco-2 cells Dab1 localizes at cell-to-cell junctions by a Ca2+-dependent process. In conclusion, the results indicate that in rodent intestine a truncated Dab1 variant transmits the reelin signal and may play a role in clathrin-mediated apical endocytosis and in the control of cell-to-cell junction assembly. A function of intestinal Dab1 variant as a nucleocytoplasmic shuttling protein is also inferred from its sequence and nuclear location.
Junta de Andalucía CTS 5884
Ministerio de Educación y Ciencia AP2007-04201
European Molecular Biology Organization ASTF45-2012
application/pdf
eng
Elsevier
Biochimica et Biophysica Acta - Biomembranes, 1860 (5), 1231-1241.
CTS 5884
AP2007-04201
ASTF45-2012
http://dx.doi.org/10.1016/j.bbamem.2018.02.014
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Cell-cell junctions
Dab1 isoform
Endocytosis
Intestine
Small and large intestine express a truncated Dab1 isoform that assembles in cell-cell junctions and co-localizes with proteins involved in endocytosis
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Fisiología
Biochimica et Biophysica Acta - Biomembranes
1860
5
1231
1241
https://ror.org/03yxnpp24
34 p.
oai:idus.us.es:11441/1470182024-02-13T20:07:47Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Koropouli, Eleftheria
Wang, Qiang
Mejías Estévez, Rebeca María
Hand, Randal
Wang, Tao
Ginty, David D.
Kolodkin, Alex L.
2023-06-07T18:17:05Z
2023-06-07T18:17:05Z
2023
Koropouli, E., Wang, Q., Mejías Estévez, R.M., Hand, R., Wang, T., Ginty, D.D. y Kolodkin, A.L. (2023). Palmitoylation regulates neuropilin-2 localization and function in cortical neurons and conveys specificity to semaphorin signaling via palmitoyl acyltransferases. eLife, 12. https://doi.org/10.7554/eLife.83217.
2050-084X
https://hdl.handle.net/11441/147018
10.7554/eLife.83217
Secreted semaphorin 3F (Sema3F) and semaphorin 3A (Sema3A) exhibit remarkably distinct effects on deep layer excitatory cortical pyramidal neurons; Sema3F mediates dendritic spine pruning, whereas Sema3A promotes the elaboration of basal dendrites. Sema3F and Sema3A signal through distinct holoreceptors that include neuropilin-2 (Nrp2)/plexinA3 (PlexA3) and neuropi-lin-1 (Nrp1)/PlexA4, respectively. We find that Nrp2 and Nrp1 are S-palmitoylated in cortical neurons and that palmitoylation of select Nrp2 cysteines is required for its proper subcellular localization, cell surface clustering, and also for Sema3F/Nrp2-dependent dendritic spine pruning in cortical neurons, both in vitro and in vivo. Moreover, we show that the palmitoyl acyltransferase ZDHHC15 is required for Nrp2 palmitoylation and Sema3F/Nrp2-dependent dendritic spine pruning, but it is dispensable for Nrp1 palmitoylation and Sema3A/Nrp1-dependent basal dendritic elaboration. Therefore, palmi-toyl acyltransferase-substrate specificity is essential for establishing compartmentalized neuronal structure and functional responses to extrinsic guidance cues.
National Institutes of Health MH100024-Project #3, R21NS085358
application/pdf
34 p.
eng
eLife Sciences Publications
eLife, 12.
MH100024-Project #3
R21NS085358
https://doi.org/10.7554/eLife.83217
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Palmitoylation regulates neuropilin-2 localization and function in cortical neurons and conveys specificity to semaphorin signaling via palmitoyl acyltransferases
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
National Institutes of Health
eLife
12
https://ror.org/03yxnpp24
oai:idus.us.es:11441/872952024-02-13T09:25:50Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Ramírez, Marco Antonio
Morales, Jorge
Cornejo, Marcelo
Blanco, Elias H.
Mancilla-Sierpe, Edgardo
Toledo, Fernando
Beltrán, Ana Rosa
Sobrevia Luarte, Luis
2019-06-10T14:38:37Z
2019-06-10T14:38:37Z
2018
Ramírez, M.A., Morales, J., Cornejo, M., Blanco, E.H., Mancilla-Sierpe, E., Toledo, F.,...,Sobrevia Luarte, L. (2018). Intracellular acidification reduces L-arginine transport via system y + L but not via system y + /CATs and nitric oxide synthase activity in human umbilical vein endothelial cells. Biochimica et Biophysica Acta - Molecular Basis of Disease, 1864 (4), 1192-1202.
0925-4439
https://hdl.handle.net/11441/87295
10.1016/j.bbadis.2018.01.032
L-Arginine is taken up via the cationic amino acid transporters (system y + /CATs) and system y + L in human umbilical vein endothelial cells (HUVECs). L-Arginine is the substrate for endothelial NO synthase (eNOS) which is activated by intracellular alkalization, but nothing is known regarding modulation of system y + /CATs and system y + L activity, and eNOS activity by the pHi in HUVECs. We studied whether an acidic pHi modulates L-arginine transport and eNOS activity in HUVECs. Cells loaded with a pH-sensitive probe were subjected to 0.1–20 mmol/L NH 4 Cl pulse assay to generate pHi 7.13–6.55. Before pHi started to recover, L-arginine transport (0–20 or 0–1000 μmol/L, 10 s, 37 °C) in the absence or presence of 200 μmol/L N-ethylmaleimide (NEM) (system y + /CATs inhibitor) or 2 mmol/L L-leucine (systemy + L substrate) was measured. Protein abundance for eNOS and serine 1177 or threonine 495 phosphorylated eNOS was determined. The results show that intracellular acidification reduced system y + L but not system y + /CATs mediated L-arginine maximal transport capacity due to reduced maximal velocity. Acidic pHi reduced NO synthesis and eNOS serine 1177 phosphorylation. Thus, system y + L activity is downregulated by an acidic pHi, a phenomenon that may result in reduced NO synthesis in HUVECs.
Fondo Nacional de Desarrollo Científico y Tecnológico 1150377
Universidad de Antofagasta 5309 , 5313
application/pdf
eng
Elsevier
Biochimica et Biophysica Acta - Molecular Basis of Disease, 1864 (4), 1192-1202.
1150377
5309
5313
http://dx.doi.org/10.1016/j.bbadis.2018.01.032
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Endothelium
Intracellular pH
L-Arginine transport
System y +
System y + L
Intracellular acidification reduces L-arginine transport via system y + L but not via system y + /CATs and nitric oxide synthase activity in human umbilical vein endothelial cells
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Biochimica et Biophysica Acta - Molecular Basis of Disease
1864
4
1192
1202
https://ror.org/03yxnpp24
11 p.
oai:idus.us.es:11441/1029532024-02-17T17:01:33Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Santana Garrido, Álvaro
Reyes Goya, Claudia
Pérez-Camino, María del Carmen
André, Helder
Mate Barrero, Alfonso
Vázquez Cueto, Carmen María
2020-12-03T13:06:48Z
2020-12-03T13:06:48Z
2020
Santana Garrido, Á., Reyes Goya, C., Pérez-Camino, M.d.C., André, H., Mate Barrero, A. y Vázquez Cueto, C.M. (2020). Retinoprotective Effect of Wild Olive (Acebuche) Oil-Enriched Diet against Ocular Oxidative Stress Induced by Arterial Hypertension. Antioxidants, 9, 1-32.
2076-3921 (electrónico)
https://hdl.handle.net/11441/102953
10.3390/antiox9090885
Oxidative stress plays an important role in the pathogenesis of ocular diseases,
including hypertensive eye diseases. The beneficial effects of olive oil on cardiovascular diseases
might rely on minor constituents. Currently, very little is known about the chemical composition
and/or therapeutic effects of the cultivated olive tree’s counterpart, wild olive (also known
in Spain as acebuche—ACE). Here, we aimed to analyze the antioxidant and retinoprotective
effects of ACE oil on the eye of hypertensive mice made hypertensive via administration of
NG-nitro-L-arginine-methyl-ester (L-NAME), which were subjected to a dietary supplementation
with either ACE oil or extra virgin olive oil (EVOO) for comparison purposes. Deep analyses of
major and minor compounds present in both oils was accompanied by blood pressure monitoring,
morphometric analyses, as well as different determinations of oxidative stress-related parameters
in retinal layers. Aside from its antihypertensive effect, an ACE oil-enriched diet reduced NADPH
(nicotinamide adenine dinucleotide phosphate) oxidase activity/gene/protein expression (with a major
implication of NADPH oxidase (NOX)2 isoform) in the retinas of hypertensive mice. Supplementation
with ACE oil in hypertensive animals also improved alterations in nitric oxide bioavailability and in
antioxidant enzyme profile. Interestingly, our findings show that the use of ACE oil resulted in better
outcomes, compared with reference EVOO, against hypertension-related oxidative retinal damage.
Consejería de Economía, Conocimiento, Empresas y Universidad, Junta de Andalucía (2017/440; 2020/275; CTS-584)
Ministerio de Ciencia e Innovación, Gobierno de España (Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020
Programa Estatal de I+D+I PID2019-109002RB-I00
application/pdf
32 p.
eng
MDPI
Antioxidants, 9, 1-32.
2017/440
2020/275
CTS-584
PID2019-109002RB-I00
https://doi.org/10.3390/antiox9090885
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Acebuche
Arterial hypertension
NADPH (nicotinamide adenine dinucleotide phosphate) oxidase
Nitric oxide
Oxidative stress
Retina
Wild olive oil
Retinoprotective Effect of Wild Olive (Acebuche) Oil-Enriched Diet against Ocular Oxidative Stress Induced by Arterial Hypertension
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Ciencia e Innovación (MICIN). España
Consejería de Economía, conocimiento, Empresas y Universidad, Junta de Andalucía
Antioxidants
9
1
32
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1489812024-02-14T11:28:50Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Romero Herrera, Inés
Nogales Bueno, Fátima
Díaz Castro, Javier
Moreno Fernández, Jorge
Gallego López, María del Carmen
Ochoa, Julio J.
Carreras Sánchez, Olimpia
Ojeda Murillo, María Luisa
2023-09-18T14:37:01Z
2023-09-18T14:37:01Z
2023
Romero Herrera, I., Nogales Bueno, F., Díaz Castro, J., Moreno Fernández, J., Gallego López, M.d.C., Ochoa, J.J.,...,Ojeda Murillo, M.L. (2023). Binge drinking leads to an oxidative and metabolic imbalance in skeletal muscle during adolescence in rats: endocrine repercussion. Journal of Physiology and Biochemistry. https://doi.org/10.1007/s13105-023-00983-z.
1138-7548
1877-8755
https://hdl.handle.net/11441/148981
10.1007/s13105-023-00983-z
Binge drinking (BD) is an especially pro-oxidant model of alcohol consumption, mainly used by adolescents. It has recently been related to the hepatic IR-process. Skeletal muscle is known to be involved in insulin action and modulation through myokine secretion. However, there is no information on muscle metabolism and myokine secretion after BD exposure in adolescents. Two experimental groups of adolescent rats have been used: control and BD-exposed one. Oxidative balance, energy status and lipid, and protein metabolism have been analyzed in muscle, together with myokine serum levels (IL-6, myostatin, LIF, IL-5, fractalkine, FGF21, irisin, BDNF, FSTL1, apelin, FABP3, osteocrin, osteonectin (SPARC), and oncostatin). In muscle, BD affects the antioxidant enzyme balance leading to lipid and protein oxidation. Besides, it also increases the activation of AMPK and thus contributes to decrease SREBP1 and pmTOR and to increase FOXO3a expressions, promoting lipid and protein degradation. These alterations deeply affect the myokine secretion pattern. This is the first study to examine a general myokine response after exposure to BD. BD not only caused a detrimental imbalance in myokines related to muscle turnover, decreased those contributing to increase IR-process, decreased FST-1 and apelin and their cardioprotective function but also reduced the neuroprotective BDNF. Consequently, BD leads to an important metabolic and energetic disequilibrium in skeletal muscle, which contributes to exacerbate a general IR-process.
Junta de Andalucía 2021/CTS-193, 2019/CTS-193
application/pdf
12 p.
eng
Springer Nature
Journal of Physiology and Biochemistry.
2021/CTS-193
2019/CTS-193
https://doi.org/10.1007/s13105-023-00983-z
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Adolescence
Binge drinking
Insulin resistance
Myokines
Skeletal muscle
Binge drinking leads to an oxidative and metabolic imbalance in skeletal muscle during adolescence in rats: endocrine repercussion
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Junta de Andalucía
Journal of Physiology and Biochemistry
https://ror.org/03yxnpp24
oai:idus.us.es:11441/853402024-02-17T17:21:19Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Carvajal Vázquez, Ana Eloisa
Vázquez Carretero, María Dolores
García Miranda, Pablo
Peral Rubio, María José
Calonge Castrillo, María Luisa
Ilundáin Larrañeta, María Anunciación Ana
2019-04-08T11:47:56Z
2019-04-08T11:47:56Z
2017
Carvajal Vázquez, A.E., Vázquez Carretero, M.D., García Miranda, P., Peral Rubio, M.J., Calonge Castrillo, M.L. y Ilundáin Larrañeta, M.A.A. (2017). Reelin expression is up-regulated in mice colon in response to acute colitis and provides resistance against colitis. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1863 (2), 462-473.
1388-1981
https://hdl.handle.net/11441/85340
10.1016/j.bbadis.2016.11.028
Reelin is an extracellular matrix protein first known for its key role in neuronal migration. Studies in rodent small intestine suggested that reelin protects the organism from intestinal pathology. Here we determined in mice colon, by real time-PCR and immunological assays, the expression of the reelin signalling system; its response to dextran sulphate sodium (DSS) and the response of wild-type and reeler mice to DSS-treatment. DNA methylation was determined by bisulfite modification and sequencing of genomic DNA. In the colon mucosa reelin expression is restricted to the myofibroblasts, whereas both epithelial cells and myofibroblasts express reelin receptors (ApoER2 and VLDLR) and its effector protein Dab1. The muscle layer also expresses reelin. DSS-treatment reduces reelin expression in the muscle but it is activated in the mucosa. Activation of mucosal reelin is greater in magnitude and is delayed until after the activation of the myofibroblasts marker, α-SMA. This indicates that the DSS-induced reelin up-regulation results from changes in the reelin gene expression rather than from myofibroblasts proliferation. DSS-treatment does not modify Sp1 or Tbr1 mRNA abundance, but increases that of TGF-β1 and ApoER2, decreases that of CASK and DNMT1 and it also decreases the reelin promoter methylation. Finally, the reeler mice exhibit higher inflammatory scores than wild-type mice, indicating that the mutation increases the susceptibility to DSS-colitis. In summary, this data are the first to demonstrate that mouse distal colon increases reelin production in response to DSS-colitis via a DNMT1-dependent hypo-methylation of the gene promoter region and that reelin provides protection against colitis
España, Junta de Andalucía CTS 5884
application/pdf
eng
Elsevier
Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1863 (2), 462-473.
CTS 5884
http://dx.doi.org/10.1016/j.bbadis.2016.11.028
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Colon
DNMT1
DSS-colitis
Reelin
Reelin expression is up-regulated in mice colon in response to acute colitis and provides resistance against colitis
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids
1863
2
462
473
https://ror.org/03yxnpp24
11 p.
oai:idus.us.es:11441/1335432024-02-13T21:58:40Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Carrascal Moreno, María Livia
Vázquez Carretero, María Dolores
García Miranda, Pablo
Fontán Lozano, Ángela del Carmen
Calonge Castrillo, María Luisa
Ilundáin Larrañeta, María Anunciación Ana
Castro, Carmen
Núñez Abades, Pedro Antonio
Peral Rubio, María José
2022-05-23T15:44:20Z
2022-05-23T15:44:20Z
2022
Carrascal Moreno, M.L., Vázquez Carretero, M.D., García Miranda, P., Fontán Lozano, Á.d.C., Calonge Castrillo, M.L., Ilundáin Larrañeta, M.A.A.,...,Peral Rubio, M.J. (2022). Acute Colon Inflammation Triggers Primary Motor Cortex Glial Activation, Neuroinflammation, Neuronal Hyperexcitability, and Motor Coordination Deficits. International Journal of Molecular Sciences, 23 (10), 5347.
1661-6596
1422-0067
https://hdl.handle.net/11441/133543
10.3390/ijms23105347
Neuroinflammation underlies neurodegenerative diseases. Herein, we test whether acute colon inflammation activates microglia and astrocytes, induces neuroinflammation, disturbs neuron intrinsic electrical properties in the primary motor cortex, and alters motor behaviors. We used a rat model of acute colon inflammation induced by dextran sulfate sodium. Inflammatory mediators and microglial activation were assessed in the primary motor cortex by PCR and immunofluorescence assays. Electrophysiological properties of the motor cortex neurons were determined by whole-cell patch-clamp recordings. Motor behaviors were examined using open-field and rotarod tests. We show that the primary motor cortex of rats with acute colon inflammation exhibited microglial and astrocyte activation and increased mRNA abundance of interleukin-6, tumor necrosis factor-alpha, and both inducible and neuronal nitric oxide synthases. These changes were accompanied by a reduction in resting membrane potential and rheobase and increased input resistance and action potential frequency, indicating motor neuron hyperexcitability. In addition, locomotion and motor coordination were impaired. In conclusion, acute colon inflammation induces motor cortex microglial and astrocyte activation and inflammation, which led to neurons’ hyperexcitability and reduced motor coordination performance. The described disturbances resembled some of the early features found in amyotrophic lateral sclerosis patients and animal models, suggesting that colon inflammation might be a risk factor for developing this disease.
Ministerio de Ciencia e Innovación 18.06.07.3005 PID2019-105632RB-I00, Grant RTI-2018.099908-B-C21
Agencia Estatal de Investigación AEI/10.13039/501100011033
application/pdf
21 p.
eng
Multidisciplinary Digital Publishing Institute (MDPI)
International Journal of Molecular Sciences, 23 (10), 5347.
18.06.07.3005 PID2019-105632RB-I00
Grant RTI-2018.099908-B-C21
AEI/10.13039/501100011033
https://doi.org/10.3390/ijms23105347
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Colon inflammation
Hyperexcitability
Microglial and astrocyte activation
Motor coordination
Motor neurons
Neurodegeneration
Neuroinflammation
Acute Colon Inflammation Triggers Primary Motor Cortex Glial Activation, Neuroinflammation, Neuronal Hyperexcitability, and Motor Coordination Deficits
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Ciencia e Innovación (MICIN). España
Agencia Estatal de Investigación. España
International Journal of Molecular Sciences
23
10
5347
https://ror.org/03yxnpp24
oai:idus.us.es:11441/523232024-02-12T21:34:16Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Benítez Temiño, Beatriz
Davis López de Carrizosa, María América
Morcuende Fernández, Sara R.
Rodríguez Matarredona, Esperanza
Rodríguez de la Cruz, Rosa María
Pastor Loro, Ángel Manuel
2017-01-17T08:43:18Z
2017-01-17T08:43:18Z
2016
Benítez Temiño, B., Davis López de Carrizosa, M.A., Morcuende Fernández, S.R., Matarredonda, E.R., Cruz, R.R.d.l. y Pastor Loro, Á.M. (2016). Functional Diversity of Neurotrophin Actions on the Oculomotor System. International Journal of Molecular Sciences, 17 (12)
1422-0067
http://hdl.handle.net/11441/52323
http://dx.doi.org/10.3390/ijms17122016
Neurotrophins play a principal role in neuronal survival and differentiation during development, but also in the maintenance of appropriate adult neuronal circuits and phenotypes. In the oculomotor system, we have demonstrated that neurotrophins are key regulators of developing and adult neuronal properties, but with peculiarities depending on each neurotrophin. For instance, the administration of NGF, BDNF or NT-3 protects neonatal extraocular motoneurons from cell death after axotomy, but only NGF and BDNF prevent the downregulation in ChAT. In the adult, in vivo recordings of axotomized extraocular motoneurons have demonstrated that the delivery of NGF, BDNF or NT-3 recovers different components of the firing discharge activity of these cells, with some particularities in the case of NGF. All neurotrophins have also synaptotrophic activity, although to different degrees. Accordingly, neurotrophins can restore the axotomy-induced alterations acting selectively on different properties of the motoneuron. In this review we summarize these evidences and discuss them in the context of other motor systems.
Ministerio de Economía y Competitividad FEDER BFU2009-07121, BFU2012-33975, BFU2015-64515-P
Junta de Andalucía-FEDER CVI-6053
application/pdf
eng
MDPI AG
International Journal of Molecular Sciences, 17 (12)
info:eu-repo/grantAgreement/MINECO/BFU2009-07121
info:eu-repo/grantAgreement/MINECO/BFU2012-33975
info:eu-repo/grantAgreement/MINECO/BFU2015-64515-P
CVI-6053
10.3390/ijms17122016
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Trophic factors
Nervous system
Plasticity
Axotomy
Functional Diversity of Neurotrophin Actions on the Oculomotor System
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Economía y Competitividad (MINECO). España
Junta de Andalucía
International Journal of Molecular Sciences
17
12
https://ror.org/03yxnpp24
34 p.
oai:idus.us.es:11441/1564242024-03-19T13:17:22Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Fuller, William
Mejías Estévez, Rebeca María
2024-03-19T13:17:22Z
2024-03-19T13:17:22Z
2023-10-30
Fuller, W. y Mejías Estévez, R.M. (2023). Editorial: Protein lipidation in health and disease. Frontiers in Physiology, 14, 1317031. https://doi.org/10.3389/fphys.2023.1317031.
1664-042X
https://hdl.handle.net/11441/156424
10.3389/fphys.2023.1317031
application/pdf
eng
Frontiers Media
Frontiers in Physiology, 14, 1317031.
https://doi.org/10.3389/fphys.2023.1317031
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
palmitoylation
lipidation
post-translational modifications
human pathologies
palmitoyl acyltransferase
Editorial: Protein lipidation in health and disease
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Frontiers in Physiology
14
1317031
oai:idus.us.es:11441/1508222024-02-13T22:27:47Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Vázquez Carretero, María Dolores
Carvajal, Ana Eloisa
Serrano Morales, José Manuel
García Miranda, Pablo
Ilundáin Larrañeta, María Anunciación Ana
Peral Rubio, María José
2023-11-16T17:00:26Z
2023-11-16T17:00:26Z
2016
Vázquez Carretero, M.D., Carvajal, A.E., Serrano Morales, J.M., García Miranda, P., Ilundáin Larrañeta, M.A.A. y Peral Rubio, M.J. (2016). The Synaptojanins in the murine small and large intestine. Journal of Bioenergetics and Biomembranes, 48 (6), 569-579. https://doi.org/10.1007/s10863-016-9689-1.
0145-479X
1573-6881
https://hdl.handle.net/11441/150822
10.1007/s10863-016-9689-1
The expression of the phosphoinositides phosphatases Synaptojanins (Synjs) 1 and 2 has been shown in brain and in some peripheral tissues, but their expression in the intestine has not been reported. Herein we show that the small and large intestine express Synj1 and Synj2. Their mRNA levels, measured by RT-PCR, are not affected by development in the small intestine but in the colon they increase with age. Immunostaining assays reveal that both Synjs localize at the apical domain of the epithelial cells and at the lamina propria at sites also expressing the neuron marker calretinin. Synj2 staining at the lamina propria is fainter than that of Synj1. In colonocytes Synjs are at the apical membrane and cytosolic membrane vesicles. Synj2 is also at the mitochondria. Western blots reveal that the intestinal mucosa expresses at least two Synj1 (170- and 139-kDa) and two Synj2 (160- and 148-kDa) isoforms. The observations suggest that Synj1–170, Synj2–160, and Synj2–148 in colonocytes, might participate in processes that take place mainly at the apical domain of the epithelial cells whereas Synj1–139 in those at the enteric nervous system. Experimental colitis augments the mRNA abundance of both Synjs in colon but only Synj2 mRNA levels are increased in colon tumors. In conclusion, as far as we know, this is the first report showing expression, location and isoforms of Synj1 and Synj2 in the small and large intestine and that they might participate in intestinal pathology.
Junta de Andalucía CTS 05884
application/pdf
22 p.
eng
Springer Nature
Journal of Bioenergetics and Biomembranes, 48 (6), 569-579.
CTS 05884
https://doi.org/10.1007/s10863-016-9689-1
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Colon
Intestine
Synaptojanin1
Synaptojanin2
The Synaptojanins in the murine small and large intestine
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Fisiología
Junta de Andalucía
Journal of Bioenergetics and Biomembranes
48
6
569
579
https://ror.org/03yxnpp24
oai:idus.us.es:11441/321412018-08-08T09:52:39Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Ilundáin Larrañeta, María Anunciación Ana
Naftalin, Richard J
2015-12-22T10:39:53Z
2015-12-22T10:39:53Z
1979
Ilundáin Larrañeta, M.A. y Naftalin, R.J. (1979). Role of Calcium-dependent regulator protein in intestinal secretion.. Nature, 279, 446-448.
0028-0836
http://hdl.handle.net/11441/32141
http://dx.doi.org/10.1038/279446a0
https://idus.us.es/xmlui/handle/11441/32141
application/pdf
eng
Macmillan
Nature, (279), 446-448
http://www.nature.com/nature/journal/v279/n5712/pdf/279446a0.pdf
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Role of Calcium-dependent regulator protein in intestinal secretion.
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Nature
279
446
448
oai:idus.us.es:11441/573372024-02-13T08:45:06Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Jovanovic, Ksenjia
Pastor Loro, Ángel Manuel
O'Donovan, Michael J.
2017-04-07T07:55:19Z
2017-04-07T07:55:19Z
2010
Jovanovic, K., Pastor Loro, Á.M. y O'Donovan, M.J. (2010). The Use of PRV-Bartha to Define Premotor Inputs to Lumbar Motoneurons in the Neonatal Spinal Cord of the Mouse. Plos One, 5 (7), e11743-1-e11743-12.
1932-6203
http://hdl.handle.net/11441/57337
10.1371/journal.pone.0011743
Background
The neonatal mouse has become a model system for studying the locomotor function of the lumbar spinal cord. However, information about the synaptic connectivity within the governing neural network remains scarce. A neurotropic pseudorabies virus (PRV) Bartha has been used to map neuronal connectivity in other parts of the nervous system, due to its ability to travel trans-neuronally. Its use in spinal circuits regulating locomotion has been limited and no study has defined the time course of labelling for neurons known to project monosynaptically to motoneurons.
Methodology/Principal Findings
Here we investigated the ability of PRV Bartha, expressing green and/or red fluorescence, to label spinal neurons projecting monosynaptically to motoneurons of two principal hindlimb muscles, the tibialis anterior (TA) and gastrocnemius (GC). As revealed by combined immunocytochemistry and confocal microscopy, 24–32 h after the viral muscle injection the label was restricted to the motoneuron pool while at 32–40 h the fluorescence was seen in interneurons throughout the medial and lateral ventral grey matter. Two classes of ipsilateral interneurons known to project monosynaptically to motoneurons (Renshaw cells and cells of origin of C-terminals) were consistently labeled at 40 h post-injection but also a group in the ventral grey matter contralaterally. Our results suggest that the labeling of last order interneurons occurred 8–12 h after motoneuron labeling and we presume this is the time taken by the virus to cross one synapse, to travel retrogradely and to replicate in the labeled cells.
Conclusions/Significance
The study establishes the time window for virally - labelling monosynaptic projections to lumbar motoneurons following viral injection into hindlimb muscles. Moreover, it provides a good foundation for intracellular targeting of the labeled neurons in future physiological studies and better understanding the functional organization of the lumbar neural networks.
Ministerio de Economía y Competitividad PET2008-0226 y BFU2009-07121
application/pdf
eng
Public Library of Science
Plos One, 5 (7), e11743-1-e11743-12.
info:eu-repo/grantAgreement/MINECO/PET2008-0226
info:eu-repo/grantAgreement/MINECO/BFU2009-07121
http://dx.doi.org/ 10.1371/journal.pone.0011743
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
The Use of PRV-Bartha to Define Premotor Inputs to Lumbar Motoneurons in the Neonatal Spinal Cord of the Mouse
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Economía y Competitividad (MINECO). España
Plos One
5
7
e11743-1
e11743-12
https://ror.org/03yxnpp24
12 p.
oai:idus.us.es:11441/676322017-12-14T18:28:48Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
González, Marcelo
Rojas, Susana
Avila, Pía
Cabrera, Lissette
Villalobos, Roberto
Palma, Carlos
Aguayo, Claudio
Sobrevia Luarte, Luis
2017-12-14T18:28:48Z
2017-12-14T18:28:48Z
2015
González, M., Rojas, S., Avila, P., Cabrera, L., Villalobos, ., Palma, C.,...,Sobrevia Luarte, L. (2015). Insulin Reverses D-Glucose–Increased Nitric Oxide and Reactive Oxygen Species Generation in Human Umbilical Vein Endothelial Cells. Plos One, 10 (4), 1-23.
1932-6203
http://hdl.handle.net/11441/67632
10.1371/journal.pone.0122398
Vascular tone is controlled by the L-arginine/nitric oxide (NO) pathway, and NO bioavailability is strongly affected by hyperglycaemia-induced oxidative stress. Insulin leads to high expression and activity of human cationic amino acid transporter 1 (hCAT-1), NO synthesis and vasodilation; thus, a protective role of insulin on high D-glucose–alterations in endothelial function is likely. Vascular reactivity to U46619 (thromboxane A2 mimetic) and calcitonin gene related peptide (CGRP) was measured in KCl preconstricted human umbilical vein rings (wire myography) incubated in normal (5 mmol/L) or high (25 mmol/L) D-glucose. hCAT-1, endothelial NO synthase (eNOS), 42 and 44 kDa mitogen-activated protein kinases (p42/44mapk), protein kinase B/Akt (Akt) expression and activity were determined by western blotting and qRT-PCR, tetrahydrobiopterin (BH4) level was determined by HPLC, and L-arginine transport (0–1000 μmol/L) was measured in response to 5–25 mmol/L D-glucose (0–36 hours) in passage 2 human umbilical vein endothelial cells (HUVECs). Assays were in the absence or presence of insulin and/or apocynin (nicotinamide adenine dinucleotide phosphate-oxidase [NADPH oxidase] inhibitor), tempol or Mn(III)TMPyP (SOD mimetics). High D-glucose increased hCAT-1 expression and activity, which was biphasic (peaks: 6 and 24 hours of incubation). High D-glucose–increased maximal transport velocity was blocked by insulin and correlated with lower hCAT-1 expression and SLC7A1 gene promoter activity. High D-glucose–increased transport parallels higher reactive oxygen species (ROS) and superoxide anion (O2•–) generation, and increased U46619-contraction and reduced CGRP-dilation of vein rings. Insulin and apocynin attenuate ROS and O2•– generation, and restored vascular reactivity to U46619 and CGRP. Insulin, but not apocynin or tempol reversed high D-glucose–increased NO synthesis; however, tempol and Mn(III)TMPyP reversed the high D-glucose–reduced BH4 level. Insulin and tempol blocked the high D-glucose–increased p42/44mapk phosphorylation. Vascular dysfunction caused by high D-glucose is likely attenuated by insulin through the L-arginine/NO and O2•–/NADPH oxidase pathways. These findings are of interest for better understanding vascular dysfunction in states of foetal insulin resistance and hyperglycaemia.
application/pdf
eng
Public Library of Science
Plos One, 10 (4), 1-23.
http://dx.doi.org/10.1371/journal.pone.0122398
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Insulin Reverses D-Glucose–Increased Nitric Oxide and Reactive Oxygen Species Generation in Human Umbilical Vein Endothelial Cells
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Plos One
10
4
1
23
23 p.
oai:idus.us.es:11441/1472122024-02-12T21:53:10Zcom_11441_11009com_11441_10983com_11441_10690com_11441_10873com_11441_10802col_11441_11010col_11441_10874
Díaz Carias, Juan Pablo
Morilla Romero de la Osa, Rubén
Cano Rodríguez, María Mercedes
2023-06-14T13:02:43Z
2023-06-14T13:02:43Z
2022
Díaz Carias, J.P., Morilla Romero de la Osa, R. y Cano Rodríguez, M.M. (2022). Relationship between insulin-biochemical resistance levels and the degree of depression and anxiety in patients from Honduras. INTERNATIONAL JOURNAL OF DIABETES IN DEVELOPING COUNTRIES, 1-8. https://doi.org/10.1007/s13410-022-01113-z.
0973-3930
1998-3832
https://hdl.handle.net/11441/147212
10.1007/s13410-022-01113-z
Background/purpose Many studies suggest that insulin resistance in obese patients bridges mental illness. Our objective was to
identify the association between levels of depression and anxiety with insulin resistance, and its relationship with obesity and
abdominal obesity
Methods A cross-sectional analytical study was carried out in Honduras. Sociodemographic variables, anthropometric param eters, HOMA index, and level of severity of anxiety and depression were collected, and a descriptive, bivariate, and multivariate
were performed.
Results In a sample of 381 adult patients, the bivariate analysis showed a statistic association of insulin resistance with all
remaining variables. However, multivariate analysis showed a significative association of anxiety with BMI, depression, waist
circumference, and insulinemia, while depression was associated with HOMA, anxiety, insulinemia, glycemia, and waist
circumference.
Conclusions Our results provide important evidence of a direct and growing association between HOMA-IR and the severity of
depression, and indirectly with anxiety. Secondarily, also with anthropometric factors (BMI and WC), traditionally associated
with cardiovascular risk. This finding has important implications both for the early diagnosis of these mental pathologies, taking
into account HOMA-IR values, and for preventive interventions focused on maintaining blood insulin levels.
application/pdf
8 p.
eng
SPRINGER INDIA
INTERNATIONAL JOURNAL OF DIABETES IN DEVELOPING COUNTRIES, 1-8.
https://link.springer.com/article/10.1007/s13410-022-01113-z
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Depression
Anxiety
Insulin Resistance
Obesity
Relationship between insulin-biochemical resistance levels and the degree of depression and anxiety in patients from Honduras
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Enfermería
Universidad de Sevilla. Departamento de Fisiología
INTERNATIONAL JOURNAL OF DIABETES IN DEVELOPING COUNTRIES
1
8
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1031592024-02-13T19:59:31Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Talaverón Aguilocho, Rocío
Rodríguez Matarredona, Esperanza
Herrera Lira, Alejandro
Medina, José M.
Tabernero, Arantxa
2020-12-12T11:55:03Z
2020-12-12T11:55:03Z
2020
Talaverón Aguilocho, R., Rodríguez Matarredona, E., Herrera Lira, A., Medina, J.M. y Tabernero, A. (2020). Connexin43 Region 266–283, via Src Inhibition, Reduces Neural Progenitor Cell Proliferation Promoted by EGF and FGF-2 and Increases Astrocytic Differentiation. International Journal of Molecular Science, 21 (22)
1422-0067
https://hdl.handle.net/11441/103159
10.3390/ijms21228852
Neural progenitor cells (NPCs) are self-renewing cells that give rise to the major cells in the nervous system and are considered to be the possible cell of origin of glioblastoma. The gap junction protein connexin43 (Cx43) is expressed by NPCs, exerting channel-dependent and -independent roles. We focused on one property of Cx43—its ability to inhibit Src, a key protein in brain development and oncogenesis. Because Src inhibition is carried out by the sequence 266–283 of the intracellular C terminus in Cx43, we used a cell-penetrating peptide containing this sequence, TAT-Cx43266–283, to explore its effects on postnatal subventricular zone NPCs. Our results show that TAT-Cx43266–283 inhibited Src activity and reduced NPC proliferation and survival promoted by epidermal growth factor (EGF) and fibroblast growth factor-2 (FGF-2). In differentiation conditions, TAT-Cx43266–283 increased astrocyte differentiation at the expense of neuronal differentiation, which coincided with a reduction in Src activity and β-catenin expression. We propose that Cx43, through the region 266–283, reduces Src activity, leading to disruption of EGF and FGF-2 signaling and to down-regulation of β-catenin with effects on proliferation and differentiation. Our data indicate that the inhibition of Src might contribute to the complex role of Cx43 in NPCs and open new opportunities for further research in gliomagenesis.
España Ministerio de Ciencia, Innovación y Universidades, Spain, FEDER RTI2018-099873-B-I00 (A.T.) and PGC20185-094654-B-100
application/pdf
22 p.
eng
International Journal of Molecular Science, 21 (22)
RTI2018-099873-B-I00 (A.T.) and PGC20185-094654-B-100
http://dx.doi.org/10.3390/ijms21228852
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
neural progenitor cells
neurons
astrocytes
ß-catenin
glioma stem cells
connexin
neural precursors
Cx43
Connexin43 Region 266–283, via Src Inhibition, Reduces Neural Progenitor Cell Proliferation Promoted by EGF and FGF-2 and Increases Astrocytic Differentiation
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Ciencia, Innovación y Universidades (MICINN). España
International Journal of Molecular Science
21
22
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1372092024-03-21T12:37:09Zcom_11441_10873com_11441_10802com_11441_10690com_11441_11044com_11441_10983col_11441_10874col_11441_11045
Santana Garrido, Álvaro
Reyes Goya, Claudia
Espinosa Martín, Pablo
Sobrevia Luarte, Luis
Beltrán Romero, Luis Matías
Vázquez Cueto, Carmen María
Mate Barrero, Alfonso
2022-09-19T15:35:43Z
2022-09-19T15:35:43Z
2022
Santana Garrido, Á., Reyes Goya, C., Espinosa Martín, P., Sobrevia Luarte, L., Beltrán Romero, L.M., Vázquez Cueto, C.M. y Mate Barrero, A. (2022). Oxidative and Inflammatory Imbalance in Placenta and Kidney of sFlt1-Induced Early-Onset Preeclampsia Rat Model. Antioxidants, 11 (8), 1608.
2076-3921
https://hdl.handle.net/11441/137209
10.3390/antiox11081608
Preeclampsia (PE) is a pregnancy-specific disorder characterized by the new onset of hypertension plus proteinuria and/or end-organ dysfunction. Here, we investigate the role of the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase system as a major component of reactive oxygen species generation, in a rodent model of early-onset preeclampsia induced by excess sFlt1 (soluble fms-like tyrosine kinase 1). Placenta and kidney samples were obtained from normal pregnant and PE rats to measure the sFlt1/PlGF (placental growth factor) ratio in addition to oxidative stress-related parameters, including the activities and expressions of NADPH oxidase isoforms (NOX1, NOX2, and NOX4), components of nitric oxide (NO) metabolism, and antioxidant enzymes. Peroxisome proliferator-activated receptors (PPARα, PPARγ) and cytokines IL1β, IL3, IL6, IL10, and IL18 were also measured to evaluate the inflammation status in our experimental setting. Excessive O2●− production was found in rats that were treated with sFlt1; interestingly, this alteration appears to be mediated mainly by NOX2 in the placenta and by NOX4 in the kidney. Altered NO metabolism and antioxidant defense systems, together with mitochondrial dysfunction, were observed in this model of PE. Preeclamptic animals also exhibited overexpression of proinflammatory biomarkers as well as increased collagen deposition. Our results highlight the role of NADPH oxidase in mediating oxidative stress and possibly inflammatory processes in the placenta and kidney of an sFlt1-based model of early-onset preeclampsia.
Junta de Andalucía PI-0456-2018, 2020/275, 2021/188, CTS-584
Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT) 1190316
São Paulo Research Foundation (FAPESP) 16/01743-5
Premio Mensual Publicación Científica Destacada de la US. Facultad de Farmacia
application/pdf
22 p.
eng
Multidisciplinary Digital Publishing Institute (MDPI)
Antioxidants, 11 (8), 1608.
PI-0456-2018
2020/275
2021/188
CTS-584
1190316
16/01743-5
https://doi.org/10.3390/antiox11081608
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Inflammation
Kidney
NADPH oxidase
Nitric oxide
Oxidative stress
Placenta
Preeclampsia
sFlt1
Oxidative and Inflammatory Imbalance in Placenta and Kidney of sFlt1-Induced Early-Onset Preeclampsia Rat Model
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. Departamento de Medicina
Junta de Andalucía
Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT). Chile
São Paulo Research Foundation (FAPESP)
Antioxidants
11
8
1608
oai:idus.us.es:11441/287322024-02-14T08:53:46Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Ojeda Murillo, María Luisa
Carreras Sánchez, Olimpia
Vázquez Cueto, Carmen María
Mate Barrero, Alfonso
2015-09-23T09:29:24Z
2015-09-23T09:29:24Z
2010
Ojeda Murillo, M.L., Carreras Sánchez, O., Vázquez Cueto, C.M. y Mate Barrero, A. (2010). Elaboración de los materiales didácticos necesarios para la adaptación de la enseñanza de hematología al Espacio Europeo de Educación Superior. Revista de Investigación Educativa, 28 (2), 313-324.
0212-4068
http://hdl.handle.net/11441/28732
https://idus.us.es/xmlui/handle/11441/28732
Con el fin de adaptarnos al Espacio Europeo de Educación Superior, este Proyecto de Innovación Docente, plantea como objetivo principal la creación, por parte del alumno, de material de autoaprendizaje basado en pruebas objetivas. La materia elegida, Hematología, forma parte de la Licenciatura de Farmacia. El proyecto, realizado sobre 400 alumnos distribuidos en 4 grupos, ha pretendido darles las bases metodológicas suficientes para elaborar preguntas de elección múltiple similares a las del sistema de evaluación del alumnado. Con esta actividad conseguimos que el alumno enfoque su aprendizaje hacia pruebas objetivas, concretando la información, distinguiendo lo más importante de cada tema, participando en el desarrollo continuo de la materia, y mejorando sus calificaciones y autoaprendizaje. El resultado de la innovación muestra un elevado grado de participación en la actividad por parte de los alumnos (incluso mayor que con otras actividades de innovación), los cuales han valorado muy positivamente la experiencia. Además, los resultados académicos han mejorado sustancialmente, como muestra la tasa de rendimiento respecto a años anteriores.
In order to comply with the European Space of Higher Educational, the main aim of this
Teaching Innovation Project is to encourage students to produce self-learning materials based on
multiple-choice tests. The course subject under consideration is Haematology, which is studied
in Pharmacy at university level. The project was carried out with 400 students distributed in 4
groups, and it aimed at providing students with enough methodological grounding, so that they
could produce multiple-choice questions similar to those used in student assessment processes.
By doing this, we managed to make students focus their learning on multiple-choice tests, thus
defining information; distinguishing the most important areas in each subject; participating
in the continuous development of the subject, and improving their marks and self-learning.
The results of this innovative process show students’ high level of participation in the activity
(even higher than in previous years), and that the students rated the experience very positively.
Furthermore, academic results improved significantly compared to previous years, as shown
by the performance rate.
application/pdf
spa
Asociación Interuniversitaria de Investigación en Pedagogía (AIDIPE)
Revista de Investigación Educativa, 28 (2), 313-324.
http://revistas.um.es/rie/article/view/100751
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Materiales didácticos
Adaptación del EEES
Hematología
Didactic materials
compliance with the European Space for Higher Educational
Haematology
Elaboración de los materiales didácticos necesarios para la adaptación de la enseñanza de hematología al Espacio Europeo de Educación Superior
info:eu-repo/semantics/article
Universidad de Sevilla. Departamento de Fisiología
Revista de Investigación Educativa
28
2
313
324
https://ror.org/03yxnpp24
oai:idus.us.es:11441/399932024-02-15T07:32:06Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Talaverón Aguilocho, Rocío
Fernández, Paola
Escamilla, Rosalba
Pastor Loro, Ángel Manuel
Rodríguez Matarredona, Esperanza
Sáez, Juan Carlos
2016-04-18T09:17:17Z
2016-04-18T09:17:17Z
2015
1662-5102
http://hdl.handle.net/11441/39993
http://dx.doi.org/10.3389/fncel.2015.00411
https://idus.us.es/xmlui/handle/11441/39993
The postnatal subventricular zone (SVZ) lining the walls of the lateral ventricles contains neural progenitor cells (NPCs) that generate new olfactory bulb interneurons. Communication via gap junctions between cells in the SVZ is involved in NPC proliferation and in neuroblast migration towards the olfactory bulb. SVZ NPCs can be expanded in vitro in the form of neurospheres that can be used for transplantation purposes after brain injury. We have previously reported that neurosphere-derived NPCs form heterocellular gap junctions with host glial cells when they are implanted after mechanical injury. To analyze functionality of NPC-glial cell gap junctions we performed dye coupling experiments in co-cultures of SVZ NPCs with astrocytes or microglia. Neurosphere-derived cells expressed mRNA for at least the hemichannel/gap junction channel proteins connexin 26 (Cx26), Cx43, Cx45 and pannexin 1 (Panx1). Dye coupling experiments revealed that gap junctional communication occurred among neurosphere cells (incidence of coupling: 100%). Moreover, hemichannel activity was also detected in neurosphere cells as evaluated in time-lapse measurements of ethidium bromide uptake. Heterocellular coupling between NPCs and glial cells was evidenced in co-cultures of neurospheres with astrocytes (incidence of coupling: 91.0 ± 4.7%) or with microglia (incidence of coupling: 71.9 ± 6.7%). Dye coupling in neurospheres and in co-cultures was inhibited by octanol, a gap junction blocker. Altogether, these results suggest the existence of functional hemichannels and gap junction channels in postnatal SVZ neurospheres. In addition, they demonstrate that SVZ-derived NPCs can establish functional gap junctions with astrocytes or microglia. Therefore, cell-cell communication via gap junctions and hemichannels with host glial cells might subserve a role in the functional integration of NPCs after implantation in the damaged brain
application/pdf
eng
Frontiers Research Foundation
Frontiers in Cellular Neuroscience, 9 (411)
10.3389/fncel.2015.00411
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Neural progenitor cells isolated from the subventricular zone present hemichannel activity and form functional gap junctions with glial cells
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1499302024-02-14T13:50:02Zcom_11441_10873com_11441_10802com_11441_10690com_11441_11094com_11441_10983com_11441_11044col_11441_10874col_11441_11095col_11441_11045
Rojas, Ángela
Lara-Romero, Carmen
Muñoz Hernández, Rocío
Gato, Sheila
Ampuero Herrojo, Javier
Romero Gómez, Manuel
2023-10-26T15:56:11Z
2023-10-26T15:56:11Z
2022
Rojas, Á., Lara-Romero, C., Muñoz Hernández, R., Gato, S., Ampuero Herrojo, J. y Romero Gómez, M. (2022). Emerging pharmacological treatment options for MAFLD. Therapeutic Advances in Endocrinology and Metabolism. https://doi.org/10.1177/20420188221142452.
2042-0188
https://hdl.handle.net/11441/149930
10.1177/20420188221142452
Metabolic dysfunction–associated fatty liver disease (MAFLD) prevalence and
incidence is rising globally. It is associated with metabolic comorbidities, obesity, overweight,
type 2 diabetes mellitus, and at least two metabolic risk factors, such as hypertension,
hypertriglyceridemia, hypercholesterolemia, insulin resistance, and cardiovascular risk,
increasing the risk of mortality. The excessive accumulation of fat comprises apoptosis,
necrosis, inflammation and ballooning degeneration progressing to fibrosis, cirrhosis, and
liver decompensations including hepatocellular carcinoma development. The limitation of
approved drugs to prevent MAFLD progression is a paradigm. This review focuses on recent
pathways and targets with evidence results in phase II/III iclinical trials.
application/pdf
eng
Therapeutic Advances in Endocrinology and Metabolism.
https://journals.sagepub.com/doi/10.1177/20420188221142452
Attribution-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nd/4.0/
info:eu-repo/semantics/openAccess
Clinical trial
Onalcoholic fatty liver disease
Pathways
Review
Therapeutics
Emerging pharmacological treatment options for MAFLD
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. Departamento de Medicina
Universidad de Sevilla. Instituto de Biomedicina de Sevilla (IBIS)
Therapeutic Advances in Endocrinology and Metabolism
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1524712023-12-14T09:58:47Zcom_11441_11019com_11441_10983com_11441_10690com_11441_10873com_11441_10802col_11441_11020col_11441_10874
Santana Garrido, Álvaro
Mate Barrero, Alfonso
Durán Lobato, María Matilde
Martín Banderas, Lucía
Vázquez Cueto, Carmen María
2023-12-14T09:58:47Z
2023-12-14T09:58:47Z
2023
Santana Garrido, Á., Mate Barrero, A., Durán Lobato, M.M., Martín Banderas, L. y Vázquez Cueto, C.M. (2023). Ophthalmic wild olive (acebuche) oil nanoemulsions exert oculoprotective effects against oxidative stress induced by arterial hypertension. International Journal of Pharmaceutics, 649, 123602. https://doi.org/10.1016/j.ijpharm.2023.123602.
0378-5173
1873-3476
https://hdl.handle.net/11441/152471
10.1016/j.ijpharm.2023.123602
Oxidative stress plays a key role in several systemic and ocular diseases, including hypertensive eye diseases. In
this context, we previously showed that oral administration of wild olive (acebuche, ACE) oil from Olea europaea
var. sylvestris can counteract ocular damage secondary to arterial hypertension by modulating excess reactive
oxygen species (ROS) produced by the enzyme nicotinamide adenine dinucleotide phosphate (NADPH) oxidase.
Therefore, this work describes the development of an ACE oil-based formulation for ocular administration as a
local therapy to counteract hypertension-related oxidative damage. Specifically, ACE oil nanoemulsions (NEs)
were successfully produced and characterized, exhibiting appropriate features for ophthalmic administration,
including a nanometer size (<200 nm), moderate negative ZP, adequate osmolality and pH, and colloidal stability in biorelevant fluids. Likewise, the NEs presented a shear thinning behavior, especially convenient for
ocular instillation. In vivo evaluation was performed through either intravitreal injection or topical ophthalmic
administration in mice with hypertension induced via administration of Nω-nitro-L-arginine-methyl-ester (LNAME). Both routes of administration reduced hypertensive morphological alterations and demonstrated a
noticeable antioxidant effect thanks to the reduction of the activity/expression of NADPH oxidase in cornea and
retina. Thus, an ACE oil ophthalmic formulation represent a promising therapy for ocular pathologies associated
with arterial hypertension.
application/pdf
18 p.
eng
Elsevier
International Journal of Pharmaceutics, 649, 123602.
https://doi.org/10.1016/j.ijpharm.2023.123602
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Acebuche oil
Cornea
L-NAME hypertension
Nanoemulsion
Oxidative stress
Retina
Ophthalmic wild olive (acebuche) oil nanoemulsions exert oculoprotective effects against oxidative stress induced by arterial hypertension
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. Departamento de Farmacia y Tecnología Farmacéutica
International Journal of Pharmaceutics
649
123602
oai:idus.us.es:11441/287802024-02-15T07:22:57Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Vázquez Cueto, Carmen María
Zanetti, Rosana
Santa-María Pérez, Consuelo
Ruiz Gutiérrez, Valentina
2015-09-23T12:28:44Z
2015-09-23T12:28:44Z
2000
0144-8463
1573-4935
http://hdl.handle.net/11441/28780
http://dx.doi.org/10.1023/A:1010377900745
https://idus.us.es/xmlui/handle/11441/28780
The effects of two monounsaturated fatty acid (MUFA) oils, olive oil (OO)and high-oleic sunflower oil (HOSO), with high content in oleic acid butdiffering in their non-fatty acid fraction, on brush-border membrane(BBM) lipid composition and fluidity and on mucosal enzyme activitiesof rat jejunum were studied. Animals were given semipurified diet withlinoleic acid to prevent essential fatty acid deficiency (control group)or semipurified diet containing 10% of either OO or HOSO for 12weeks. There was a significant decrease in the content of jejunalBBM phospholipids together with an increase in the level of freecholesterol in both oil-fed rats, when compared to controlgroup. Although the increase in the BBM free cholesterol levelwas not statistically significant in HOSO-fed rats, a significantdecrease in the phospholipid/free cholesterol ratio was found inboth OO and HOSO-fed animals compared to control group. Rat jejunalBBM had a high level of free fatty acids which was increased in BBMisolated from OO and HOSO-fed animals. There was no statisticalsignificant difference in the phospholipid distribution between thecontrol and the OO group. However, HOSO-fed animals showed the lowestlevel of phosphatidylethanolamine together with the highestphosphatidylcholine content and the phosphatidylcholine/sphingomyelinratio. The fatty acid pattern of jejunal BBM lipids was modifiedaccording to the major fatty acids in the oils. There was a decreasein both stearic acid (18:0) and linoleic acid (18:2 n-6), togetherwith an increase in oleic acid (18:1 n-9) in jenunal BBM isolatedfrom both oil experimental groups. All these results were accompaniedby a significant increase in the BBM fluidity (as assessed bysteady-state fluorescence polarization of diphenylhexatriene) isolatedfrom oil-fed rat, when compared to control group. OO and HOSO-fedanimals had the lowest activities of sucrase and maltase, whilealkaline phosphatase activity only was decreased in HOSO-fedanimals. The specific activity of maltase was not modified in anyexperimental rats. In summary, both MUFA oils induced similar effectson jejunal BBM lipid composition, fluidity, sucrase, maltase andlactase activities. Furthermore, HOSO intake resulted in a lowestalkaline phosphatase activity which was accompanied by changes inindividual phospholipid composition. All these results suggest thateffects of MUFA oils on jejunal BBM lipid composition and hydrolaseactivities are most likely due to the presence of high content ofoleic acid rather than other components contained in the non-fattyacid of olive oil.
application/pdf
eng
Portland Press
Bioscience reports, 20(5), 355-368
http://dx.doi.org/10.1023/A:1010377900745
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Oleic acid
olive oil
lipid composition
hydrolases
jejunum
rat
Effects of two highly monounsaturated oils on lipid composition and enzyme activities in rat jejunum
info:eu-repo/semantics/article
Universidad de Sevilla. Departamento de Fisiología
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1490872024-02-13T22:06:27Zcom_11441_10873com_11441_10802com_11441_10690com_11441_11044com_11441_10983col_11441_10874col_11441_11045
Gil Gómez, Antonio
Rojas, Ángela
García Lozano, María R.
Muñoz Hernández, Rocío
Gallego-Durán, Rocío
Maya Miles, Douglas
Ampuero Herrojo, Javier
Romero Gómez, Manuel
2023-09-21T16:59:27Z
2023-09-21T16:59:27Z
2022
Gil Gómez, A., Rojas, Á., García Lozano, M.R., Muñoz Hernández, R., Gallego-Durán, R., Maya Miles, D.,...,Romero Gómez, M. (2022). Impact of a Loss-of-Function Variant in HSD17B13 on Hepatic Decompensation and Mortality in Cirrhotic Patients. International Journal of Molecular Sciences, 23 (19), 11840. https://doi.org/10.3390/ijms231911840.
1422-0067
https://hdl.handle.net/11441/149087
10.3390/ijms231911840
Abstract: A common splice variant in HSD17B13 (rs72613567:TA) was recently found to be associated
with a reduced risk of developing chronic liver disease in NAFLD patients and a reduced risk of
progression to advanced fibrosis and cirrhosis. In this study, we aimed to evaluate the prognosis of
cirrhotic patients harboring this variant. We performed a retrospective analysis on 483 prospectively
recruited patients from four different hospitals in Spain, followed-up for at least 5 years. We collected
clinical, demographic, and biochemical data, and we performed a genotyping analysis for com mon variants previously associated with liver disease risk (HSD17B13 rs72613567:TA and PNPLA3
rs738409). Patients homozygous for the TA allele showed a higher MELD score (p = 0.047), Child–
Turcotte–Pugh score (p = 0.014), and INR levels (p = 0.046), as well as decreased albumin (p = 0.004) at
baseline. After multivariate analysis, patients with the “protective” variant indeed had an increased
risk of hepatic decompensation [aHR 2.37 (1.09–5.06); p = 0.029] and liver-related mortality [aHR 2.32
(1.20–4.46); p = 0.012]. Specifically, these patients had an increased risk of developing ascites (Log-R
11.6; p < 0.001), hepatic encephalopathy (Log-R 10.2; p < 0.01), and higher mortality (Log-R 14.1;
p < 0.001) at 5 years of follow-up. Interactions with the etiology of the cirrhosis and with the variant
rs738409 in PNPLA3 are also described. These findings suggest that the variant rs72613567:TA in
HSD17B13 has no protective effect, but indeed increases the risk of decompensation and death in
patients with advanced chronic liver disease.
application/pdf
11
eng
MDPI AG
International Journal of Molecular Sciences, 23 (19), 11840.
PE-0451-2018 ,P20_01075
PI19/01404, PI19/00589
https://www.mdpi.com/1422-0067/23/19/11840
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Cirrhosis
Polymorphism
PNPLA3
HSD17B13
NAFLD
Fibrosis
Ascites
Hepatic encephalopathy
SNP
Hepatic decompensation
Impact of a Loss-of-Function Variant in HSD17B13 on Hepatic Decompensation and Mortality in Cirrhotic Patients
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Medicina
Universidad de Sevilla. Departamento de Fisiología
Consejería de Salud, Junta de Andalucía
Ministerio de Ciencia e Innovación
Junta de Andalucía
International Journal of Molecular Sciences
23
19
11840
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1527892024-02-13T20:24:33Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Nogales Bueno, Fátima
Ojeda Murillo, María Luisa
Serrano, A.
Rua, R. M.
Carreras Sánchez, Olimpia
2023-12-26T09:51:48Z
2023-12-26T09:51:48Z
2021-03
Nogales Bueno, F., Ojeda Murillo, M.L., Serrano, A., Rua, R.M. y Carreras Sánchez, O. (2021). Metabolic syndrome during gestation and lactation: an important renal problem in dams. selenium renal clearance. Journal of Trace Elements in Medicine and Biology, 64, 126709. https://doi.org/10.1016/j.jtemb.2020.126709.
0946-672X
1878-3252
https://hdl.handle.net/11441/152789
10.1016/j.jtemb.2020.126709
Background: Metabolic syndrome (MS) in lactating dams leads to several cardiometabolic changes related to
selenium (Se) status and selenoproteins expression which produce hypertension. However, little is known about
the state of these dams’ kidney functions and their Se deposits.
Methods: Two experimental groups of dam rats were used: control (Se: 0.1 ppm) and MS (Fructose 65 % and Se:
0.1 ppm). At the end of lactation (21d postpartum) kidney weight and protein content, Se deposits, and the
activity of the antioxidant selenoprotein glutathione peroxidase (GPx) were measured in dams. Kidney functional
parameters: albuminuria, creatinine clearance, serum aldosterone and uric acid levels and water and electrolyte
(Na+ and K+) balance were also evaluated. Systolic blood pressure (SBP) was measured.
Results: In MS dams at the end of lactation Se deposits and GPx activity are higher in the kidney; however, lipid
renal peroxidation appears, relative Se clearance increases, and the dams have lost Se by urine. MS dams have
polyuria and polydipsia, high uric acid serum levels, albuminuria and high creatinine clearance, implying
glomerular renal malfunction with protein loss. They also present hypernatremia, hypokalemia and hyperaldosteronemia, leading to high SBP; however, a natriuretic process is taking place.
Conclusion: Since these alterations appear, at least in part, to be related to oxidative stress in renal cells, Se
supplementation could be beneficial to avoiding greater lipid renal oxidation during lactation.
Junta de Andalucía - CTS-193
application/pdf
6 p.
eng
Elsevier
Journal of Trace Elements in Medicine and Biology, 64, 126709.
CTS-193
https://doi.org/10.1016/j.jtemb.2020.126709
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Metabolic syndrome
Selenium
Renal damage
Systolic blood pressure
Metabolic syndrome during gestation and lactation: an important renal problem in dams. selenium renal clearance
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. CTS193: Implicación del Balance Oxidativo en la Salud: Alcoholismo y Síndrome Metabólico.
Journal of Trace Elements in Medicine and Biology
64
126709
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1516872024-03-15T10:40:31Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Carrero Rojas, Génova
García Hernández, Rosendo Miguel
Blumer, Roland
Rodríguez de la Cruz, Rosa María
Pastor Loro, Ángel Manuel
2023-11-27T16:39:42Z
2023-11-27T16:39:42Z
2021
Carrero Rojas, G., García Hernández, R.M., Blumer, R., Rodríguez de la Cruz, R.M. y Pastor Loro, Á.M. (2021). MIF versus SIF motoneurons, what are their respective contribution in the oculomotor medial rectus pool?. Journal of Neuroscience, 41 (47), 9782-9793. https://doi.org/10.1523/JNEUROSCI.1480-21.2021.
0270-6474
1529-2401
https://hdl.handle.net/11441/151687
10.1523/JNEUROSCI.1480-21.2021
Multiply-innervated muscle fibers (MIFs) are peculiar to the extraocular muscles as they are non-twitch but produce a slow build up in tension on repetitive stimulation. The motoneurons innervating MIFs establish en grappe terminals along the entire length of the fiber, instead of the typical en plaque terminals that singly-innervated muscle fibers (SIFs) motoneurons establish around the muscle belly. MIF motoneurons have been proposed to participate only in gaze holding and slow eye movements. We aimed to discern the function of MIF motoneurons by recording medial rectus motoneurons of the oculomotor nucleus. Single-unit recordings in awake cats demonstrated that electrophysiologically-identified medial rectus MIF motoneurons participated in different types of eye movements, including fixations, rapid eye movements or saccades, convergences, and the slow and fast phases of the vestibulo-ocular nystagmus, the same as SIF motoneurons did. However, MIF medial rectus motoneurons presented lower firing frequencies, were recruited earlier and showed lower eye position (EP) and eye velocity (EV) sensitivities than SIF motoneurons. MIF medial rectus motoneurons were also smaller, had longer antidromic latencies and a lower synaptic coverage than SIF motoneurons. Peristimulus time histograms (PSTHs) revealed that electrical stimulation to the myotendinous junction, where palisade endings are located, did not recurrently affect the firing probability of medial rectus motoneurons. Therefore, we conclude there is no division of labor between MIF and SIF motoneurons based on the type of eye movement they subserve.
Ministerio de Ciencia e Innovación PGC2018-094654-B-100
Austrian Science P32463-B
Premio Mensual Publicación Científica Destacada de la US. Facultad de Biología
application/pdf
45 p.
eng
Society for Neuroscience
Journal of Neuroscience, 41 (47), 9782-9793.
PGC2018-094654-B-100
P32463-B
https://doi.org/10.1523/JNEUROSCI.1480-21.2021
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Eye movements
Motoneuron
Multiply innervated fiber
Oculomotor system
Palisade endings
Singly innervated fiber
MIF versus SIF motoneurons, what are their respective contribution in the oculomotor medial rectus pool?
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Ciencia e Innovación (MICIN). España
Austrian Science Fund
Journal of Neuroscience
41
47
9782
9793
https://ror.org/03yxnpp24
oai:idus.us.es:11441/166832024-02-17T16:59:04Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Universidad de Sevilla. Departamento de Fisiología
Carmona Zalvide, Pilar
García García, Francisco José
2014-11-27T12:29:54Z
2014-11-27T12:29:54Z
2000
Carmona Zalvide, P. y García García, F.J. (2000). Moluscos poliplacóforos del litoral atlántico del sur de la península ibérica. Graellsia, 56, 5-14.
0367-5041
http://graellsia.revistas.csic.es/index.php/graellsia/article/viewFile/304/294
http://hdl.handle.net/11441/16683
https://idus.us.es/xmlui/handle/11441/16683
Se aporta el catálogo de los Moluscos Poliplacóforos de las costas atlánticas del sur
de la Península Ibérica, desde Sagres (Portugal) hasta Gibraltar. Se cita un total de 20
taxones (Lepidopleurus cajetanus, Leptochiton cancellatus, Leptochiton algesirensis,
Leptochiton scabridus, Callochiton septemvalvis, Callochiton euplaeae, Lepidochitona
cinerea, Lepidochitona corrugata, Lepidochitona canariensis, Lepidochitona monterosatoi,
Lepidochitona kaasi, Lepidochitona severianoi, Chaetopleura angulata,
Ischnochiton rissoi, Chiton olivaceus, Chiton corallinus, Chiton phaesolinus,
Acanthochi-tona fascicularis y Acanthochitona crinita) todos ellos pertenecientes al
dominio litoral. La captura de Lepidochitona canariensis y L. simrothi en aguas atlánticas
ibéricas constituye la primera cita para el suratlántico ibérico. A su vez se amplía la
distribución a esta zona de Callochiton septemvalvis y de Lepidochitona monterosatoi.
In this paper, an updated check-list of the polyplacophoran species from Sagres
(Portugal) to Strait of Gibraltar is present. Twenty taxa are recorded in this area:
Lepidopleurus cajetanus, Leptochiton cancellatus, Leptochiton algesirensis, Leptochiton
scabridus, Callochiton septemvalvis, Callochiton euplaeae, Lepidochitona cinerea,
Lepidochitona corrugata, Lepidochitona canariensis, Lepidochitona monterosatoi,
Lepidochitona kaasi, Lepidochitona severianoi, Chaetopleura angulata, Ischnochiton
rissoi, Chiton olivaceus, Chiton corallinus, Chiton phaesolinus, Acanthochitona fascicularis
and Acanthochitona crinita. From these species, Lepidochitona canariensis, L simrothi,
Callochiton septemvalvis and Lepidochitona monterosatoi amplify their geographical
distribution to Southern Atlantic coast of southern Iberian Peninsula
spa
Graellsia, 56, 5-14.
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Molluscan Polyplacophora
check-list
Atlantic coast
SW Iberian Peninsula
Moluscos poliplacóforos
catálogo faunístico
distribución litoral
SO Ibérico
Moluscos poliplacóforos del litoral atlántico del sur de la península ibérica
info:eu-repo/semantics/article
Graellsia
56
5
14
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1524162024-02-12T21:34:39Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Santana Garrido, Álvaro
Reyes Goya, Claudia
Andre, Helder
Vázquez Cueto, Carmen María
Mate Barrero, Alfonso
2023-12-12T12:39:51Z
2023-12-12T12:39:51Z
2023
Santana Garrido, Á., Reyes Goya, C., Andre, H., Vázquez Cueto, C.M. y Mate Barrero, A. (2023). Exploring the Potential of Wild Olive (Acebuche) Oil as a Pharm-Food to Prevent Ocular Hypertension and Fibrotic Events in the Retina of Hypertensive Mice. Molecular Nutrition & Food Research, 2023, e2200623. https://doi.org/10.1002/mnfr.202200623.
1613-4125
1613-4133
https://hdl.handle.net/11441/152416
10.1002/mnfr.202200623
Scope Our laboratory has previously described the antioxidant and anti-inflammatory potential of a wild olive (acebuche, ACE) oil against hypertension-associated vascular retinopathies. The current study aims to analyze the antifibrotic effect of ACE oil on the retina of hypertensive mice.
Methods and results Mice are rendered hypertensive by administration of NG-nitro-L-arginine-methyl-ester (L-NAME) and simultaneously subjected to dietary supplementation with ACE oil or a reference extra virgin olive oil (EVOO). Intraocular pressure (IOP) is measured by rebound tonometry, and retinal vasculature/layers are analyzed by fundus fluorescein angiography and optical coherence tomography. Different fibrosis-related parameters are analyzed in the retina and choroid of normotensive and hypertensive mice with or without oil supplementation. Besides preventing the alterations found in hypertensive animals, including increased IOP, reduced fluorescein signal, and altered retinal layer thickness, the ACE oil-enriched diet improves collagen metabolism by regulating the expression of major fibrotic process modulators (matrix metalloproteinases, tissue inhibitors of metalloproteinases, connective tissue growth factor, and transforming growth factor beta family).
Conclusion Regular consumption of EVOO and ACE oil (with better outcomes in the latter) might help reduce abnormally high IOP values in the context of hypertension-related retinal damage, with significant reduction in the surrounding fibrotic process.
MCIN/AEI/10.13039/501100011033 - PID2019-109002RB-I00
Junta de Andalucía, Consejería de Universidad, Investigación e Innovación, Secretaría General de Investigación e Innovación - ProyExcel_00516
application/pdf
16 p.
eng
Wiley
Molecular Nutrition & Food Research, 2023, e2200623.
PID2019-109002RB-I00
ProyExcel_00516
https://doi.org/10.1002/mnfr.202200623
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Exploring the Potential of Wild Olive (Acebuche) Oil as a Pharm-Food to Prevent Ocular Hypertension and Fibrotic Events in the Retina of Hypertensive Mice
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Ciencia e Innovación (MICIN). España
Agencia Estatal de Investigación. España
Junta de Andalucía
Molecular Nutrition & Food Research
2023
e2200623
https://ror.org/03yxnpp24
oai:idus.us.es:11441/362392024-02-12T22:09:08Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Vázquez Cueto, Carmen María
Zanetti, Rosana
Ruiz Gutiérrez, Valentina
2016-02-22T11:38:36Z
2016-02-22T11:38:36Z
1996
0144-8463
http://hdl.handle.net/11441/36239
http://dx.doi.org/10.1007/BF01207336
https://idus.us.es/xmlui/handle/11441/36239
The lipid compositíon and fluidity of jejunal brush-border membrane vesicles (BBMV) have been studied in spontaneously hypertensive rats (SHR) and normotensive Wistar Kyoto (WKY) rats. The activities of both Na+-dependent D-glucose cotransport and Na+-H antiport have also been determined. A significan! increase in the level of free cholesterol was observed in jejunal BBMV from SHR compared to WKY rats. Since phospholipid values did not change in either group of animals, a significan! enhancement in the free cholesterol/phospholipid ratio was observed in SHR. A decrease in the levels of phosphatidylethanolamine together with an increase in the values of phos phatidylserine was observed in hypertensive rats. Although the content of phosphatidylcholine (PC) and sphingomyelin (SM) was not singificantly altered in SHR, the ratio PC/SM significantly increased in these animals when compared to WKY rats. The majar fatty acids present in bursh-border membranes prepared from SHR and WKY rats were palmitic (16:0), stearic (18:0), oleic (18:1, n-9) and linoleic (18:2, n-6), and the fatty acid composition was not modified by the hypertension. A decreased fluorescence polarization, i.e., increased membrane ftuidity, was observed in SHR, which was not correlated to the increased ratio of cholesterol/phospholipid found in the brush-border membrane isolated from these animals. These structural changes found in SHR were associated to an enhancement in both Na+ -dependent D-glucose transport and Na+-H+ antiport activíty in the jejunal BBMVof SHR.
application/pdf
eng
Portland Press Ltd
Bioscience Reports, 16, 217-226
10.1007/BF01207336
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Spontaneously hypertensive rat
Brush-border membrane
Lipid ftuídity
Lípíd composition
D-glucose
Na+ -H+ exchanger
Lipid Composition and Fluidity in the Jejunal Brush-Border Membrane of Spontaneously Hypertensive Rats. Effects on Activities of Membrane- Bound Proteins
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
https://ror.org/03yxnpp24
oai:idus.us.es:11441/498772024-02-17T17:37:59Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Carreras Sánchez, Olimpia
Murillo Taravillo, María Luisa
Delgado Guerrero, María Josefa
Bolufer González, José
2016-12-07T13:41:15Z
2016-12-07T13:41:15Z
1988
Carreras Sánchez, O., Murillo Taravillo, M.L., Delgado Guerrero, M.J. y Bolufer González, J. (1988). Leucine absorption after jejunoileal bypass in rats. Revista española de fisiología, 44 (3), 295-302.
0034-9402
http://hdl.handle.net/11441/49877
https://idus.us.es/xmlui/handle/11441/49877
Jejunal and ileal absorption of L-leucine has been studied in rats subjected to jejunoileal
bypass and in sham-operated rats, for five minute periods, using a perfusion technique. Aminoacid concentrations were: 1, 2.5, 5, 10 and 25 mM. In sorne experiments methionine was added to determine simple diffusion. The ratio of the active/diffusive components of absorption were calculated at the different luminal aminoacid concentrations in both groups of rats, showing that this ratio was lower in control animals
application/pdf
eng
Asociación Española de Toxicología
Revista española de fisiología, 44 (3), 295-302.
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Rat
Leucine
Intestinal absorption
Intestinal bypass
Leucine absorption
Leucine absorption after jejunoileal bypass in rats
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Revista española de fisiología
44
3
295
302
https://ror.org/03yxnpp24
8 p.
oai:idus.us.es:11441/1025552024-02-14T20:16:19Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Sanhueza, Carlos
Araos, Joaquín D.
Naranjo, Luciano
Barros, Eric
Subiabre, Mario
Toledo, Fernando
Gutiérrez, Jaime Agustín
Chiarello, Delia Indira
Pardo, Fabián N.
Leiva, Andrea
Sobrevia Luarte, Luis
2020-11-10T15:11:05Z
2020-11-10T15:11:05Z
2016
Sanhueza, C., Araos, J.D., Naranjo, L., Barros, E., Subiabre, M., Toledo, F.,...,Sobrevia Luarte, L. (2016). Nitric oxide and pH modulation in gynaecological cancer. Journal of Cellular and Molecular Medicine, 20 (12), 2223-2230.
1582-1838
1582-4934
https://hdl.handle.net/11441/102555
10.1111/jcmm.12921
Nitric oxide plays several roles in cellular physiology, including control of the vascular tone and defence against pathogen infection. Neuronal, inducible and endothelial nitric oxide synthase (NOS) isoforms synthesize nitric oxide. Cells generate acid and base equivalents, whose physiological intracellular concentrations are kept due to membrane transport systems, including Na+/H+ exchangers and Na+/HCO3 − transporters, thus maintaining a physiological pH at the intracellular (~7.0) and extracellular (~7.4) medium. In several pathologies, including cancer, cells are exposed to an extracellular acidic microenvironment, and the role for these membrane transport mechanisms in this phenomenon is likely. As altered NOS expression and activity is seen in cancer cells and because this gas promotes a glycolytic phenotype leading to extracellular acidosis in gynaecological cancer cells, a pro-inflammatory microenvironment increasing inducible NOS expression in this cell type is feasible. However, whether abnormal control of intracellular and extracellular pH by cancer cells regards with their ability to synthesize or respond to nitric oxide is unknown. We, here, discuss a potential link between pH alterations, pH controlling membrane transport systems and NOS function. We propose a potential association between inducible NOS induction and Na+/H+ exchanger expression and activity in human ovary cancer. A potentiation between nitric oxide generation and the maintenance of a low extracellular pH (i.e. acidic) is proposed to establish a sequence of events in ovarian cancer cells, thus preserving a pro-proliferative acidic tumour extracellular microenvironment. We suggest that pharmacological therapeutic targeting of Na+/H+ exchangers and inducible NOS may have benefits in human epithelial ovarian cancer.
Fondo Nacional de Desarrollo Científico y Tecnológico 3140516, 1150377, 1150344, 3160194, 11150083
application/pdf
8 p.
eng
Wiley-Blackwell
Journal of Cellular and Molecular Medicine, 20 (12), 2223-2230.
3140516
1150377
1150344
3160194
11150083
https://doi.org/10.1111/jcmm.12921
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
NHE
Nitric oxide
Ovarian cancer
pH
Nitric oxide and pH modulation in gynaecological cancer
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Journal of Cellular and Molecular Medicine
20
12
2223
2230
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1078222024-02-15T07:34:37Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Geribaldi Doldán, Noelia
Hervás Corpión, Irati
Gómez Oliva, Ricardo
Domínguez García, Samuel
Ruiz, Felix A.
Iglesias Lozano, Irene
Carrascal Moreno, María Livia
Nuñez Abades, Pedro Antonio
2021-04-26T11:53:16Z
2021-04-26T11:53:16Z
2021
Geribaldi Doldán, N., Hervás Corpión, I., Gómez Oliva, R., Domínguez García, S., Ruiz, F.A., Iglesias Lozano, I.,...,Nuñez Abades, P.A. (2021). Targeting Protein Kinase C in Glioblastoma Treatment. Biomedicines, 9 (4), 381.
2227-9059
https://hdl.handle.net/11441/107822
http://dx.doi.org/10.3390/biomedicines9040381
Glioblastoma (GBM) is the most frequent and aggressive primary brain tumor and is associated with a poor prognosis. Despite the use of combined treatment approaches, recurrence is almost inevitable and survival longer than 14 or 15 months after diagnosis is low. It is therefore necessary to identify new therapeutic targets to fight GBM progression and recurrence. Some publications have pointed out the role of glioma stem cells (GSCs) as the origin of GBM. These cells, with characteristics of neural stem cells (NSC) present in physiological neurogenic niches, have been proposed as being responsible for the high resistance of GBM to current treatments such as temozolomide (TMZ). The protein Kinase C (PKC) family members play an essential role in transducing signals related with cell cycle entrance, differentiation and apoptosis in NSC and participate in distinct signaling cascades that determine NSC and GSC dynamics. Thus, PKC could be a suitable druggable target to treat recurrent GBM. Clinical trials have tested the efficacy of PKCβ inhibitors, and preclinical studies have focused on other PKC isozymes. Here, we discuss the idea that other PKC isozymes may also be involved in GBM progression and that the development of a new generation of effective drugs should consider the balance between the activation of different PKC subtypes.
Integrated Territorial Investment Operational Programme of the European Commission and by the Department of Department of Health and Families (Consejería de Salud y Familias) of the Regional Government of Andalusia. Project reference: ITI0042-2019: ITI Cadiz 2019
application/pdf
22 p.
eng
MDPI
Biomedicines, 9 (4), 381.
ITI0042-2019: ITI Cadiz 2019
10.3390/biomedicines9040381
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
glioblastoma
temozolomide
enzastaurin
epidermal growth factor receptor
neuregulin
protein kinase C
glioma stem cells
neurogenesis
neural stem cells
Targeting Protein Kinase C in Glioblastoma Treatment
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Biomedicines
9
4
381
https://ror.org/03yxnpp24
oai:idus.us.es:11441/747382024-02-17T17:15:23Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Miguel Carrasco, José Luis
Beaumont, Javier
San José, Gorka
Moreno, María U.
2018-05-17T10:21:12Z
2018-05-17T10:21:12Z
2017
Miguel Carrasco, J.L., Beaumont, J., San José, G. y Moreno, M.U. (2017). Mechanisms underlying the cardiac antifibrotic effects of losartan metabolites. Scientific Reports, 7 (41865), 1-9.
2045-2322
https://hdl.handle.net/11441/74738
10.1038/srep41865
Excessive myocardial collagen deposition and cross-linking (CCL), a process regulated by lysyl oxidase (LOX), determines left ventricular (LV) stiffness and dysfunction. The angiotensin II antagonist losartan, metabolized to the EXP3179 and EXP3174 metabolites, reduces myocardial fibrosis and LV stiffness in hypertensive patients. Our aim was to investigate the differential influence of losartan metabolites on myocardial LOX and CCL in an experimental model of hypertension with myocardial fibrosis, and whether EXP3179 and EXP3174 modify LOX expression and activity in fibroblasts. In rats treated with NG-nitro-L-arginine methyl ester (L-NAME), administration of EXP3179 fully prevented LOX, CCL and connective tissue growth factor (CTGF) increase, as well as fibrosis, without normalization of blood pressure (BP). In contrast, administration of EXP3174 normalized BP and attenuated fibrosis but did not modify LOX, CCL and CTGF. In TGF-β1-stimulated fibroblasts, EXP3179 inhibited CTGF and LOX expression and activity with lower IC50 values than EXP3174. Our results indicate that, despite a lower antihypertensive effect, EXP3179 shows higher anti-fibrotic efficacy than EXP3174, likely through its ability to prevent the excess of LOX and CCL. It is suggested that the anti-fibrotic effect of EXP3179 may be partially mediated by the blockade of CTGF-induced LOX in fibroblasts.
España, Ministerio de Economia y Competitividad SAF2011-29610, SAF2013-49088-R
application/pdf
eng
Nature Publishing Group:
Scientific Reports, 7 (41865), 1-9.
SAF2011-29610
SAF2013-49088-R
http://dx.doi.org/ 10.1038/srep41865
Atribución-NoComercial-SinDerivadas 3.0 Estados Unidos de América
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Mechanisms underlying the cardiac antifibrotic effects of losartan metabolites
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Economía y Competitividad (MINECO). España
Scientific Reports
7
41865
1
9
https://ror.org/03yxnpp24
9 p.
oai:idus.us.es:11441/230322024-02-13T09:33:04Zcom_11441_10873com_11441_10802com_11441_10690com_11441_10853col_11441_10874col_11441_10854
Gaytán Guía, Susana Pilar
Pontiga Romero, Francisco de Paula
2015-02-27T12:17:48Z
2015-02-27T12:17:48Z
2005
Gaytán Guía, S.P. y Pontiga Romero, F.d.P. (2005). An experimental approach to the fundamental principles of hemodynamics. Advances in Physiology Education, 29 (3), 165-171.
1043-4046
http://advan.physiology.org/content/ajpadvan/29/3/165.full.pdf
http://hdl.handle.net/11441/23032
10.1152/advan.00009.2005
https://idus.us.es/xmlui/handle/11441/23032
eng
Advances in Physiology Education, 29(3), 165-171
http://dx.doi.org/10.1152/advan.00009.2005
Atribución-NoComercial-SinDerivadas 4.0 España
http://creativecommons.org/licenses/by-nc-nd/4.0
info:eu-repo/semantics/openAccess
An experimental approach to the fundamental principles of hemodynamics
info:eu-repo/semantics/article
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. Departamento de Física Aplicada II
Advances in Physiology Education
29
3
165
171
https://ror.org/03yxnpp24
oai:idus.us.es:11441/780382024-02-14T19:27:56Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Acosta, Lourdes
Morcuende Fernández, Sara R.
Silva Hucha, Silvia
Pastor Loro, Ángel Manuel
Rodríguez de la Cruz, Rosa María
2018-08-13T11:24:47Z
2018-08-13T11:24:47Z
2018
Acosta, L., Morcuende Fernández, S.R., Silva Hucha, S., Pastor Loro, Á.M. y Rodríguez de la Cruz, R.M. (2018). Vascular Endothelial Growth Factor (VEGF) Prevents the Downregulation of the Cholinergic Phenotype in Axotomized Motoneurons of the Adult Rat. Frontiers in Molecular Neuroscience, 11 (241)
1662-5099)
https://hdl.handle.net/11441/78038
10.3389/fnmol.2018.00241
Vascular endothelial growth factor (VEGF) was initially characterized by its activity on the vascular system. However, there is growing evidence indicating that VEGF also acts as a neuroprotective factor, and that its administration to neurons suffering from trauma or disease is able to rescue them from cell death. We questioned whether VEGF could also maintain damaged neurons in a neurotransmissive mode by evaluating the synthesis of their neurotransmitter, and whether its action would be direct or through its well-known angiogenic activity. Adult rat extraocular motoneurons were chosen as the experimental model. Lesion was performed by monocular enucleation and immediately a gelatine sponge soaked in VEGF was implanted intraorbitally. After 7 days, abducens, trochlear, and oculomotor nuclei were examined by immunohistochemistry against choline acetyltransferase (ChAT), the biosynthetic enzyme of the motoneuronal neurotransmitter acetylcholine. Lesioned motoneurons exhibited a noticeable ChAT downregulation which was prevented by VEGF administration. To explore whether this action was mediated via an increase in blood vessels or in their permeability, we performed immunohistochemistry against laminin, glucose transporter-1 and the plasmatic protein albumin. The quantification of the immunolabeling intensity against these three proteins showed no significant differences between VEGF-treated, axotomized and control animals. Therefore, the present data indicate that VEGF is able to sustain the cholinergic phenotype in damaged motoneurons, which is a first step for adequate neuromuscular neurotransmission, and that this action seems to be mediated directly on neurons since no sign of angiogenic activity was evident. These data reinforces the therapeutical potential of VEGF in motoneuronal diseases.
España, MINECO and FEDER BFU2015-64515-P
Junta de Andalucía and FEDER : P10-CVI6053
application/pdf
eng
Frontiers Media
Frontiers in Molecular Neuroscience, 11 (241)
BFU2015-64515-P
http://dx.doi.org/10.3389/fnmol.2018.00241
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
VEGF
axotomy
oculomotor system
amyotrophic lateral sclerosis
angiogenesis
laminin
glucose transporter 1 (GLUT-1)
acetylcholine
Vascular Endothelial Growth Factor (VEGF) Prevents the Downregulation of the Cholinergic Phenotype in Axotomized Motoneurons of the Adult Rat
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Economía y Competitividad (MINECO). España
Junta de Andalucía
Frontiers in Molecular Neuroscience
11
241
https://ror.org/03yxnpp24
oai:idus.us.es:11441/961372024-02-13T10:04:57Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Domínguez García, Samuel
Geribaldi Doldán, Noelia
Gómez Oliva, Ricardo
Ruiz, Felix A.
Carrascal Moreno, María Livia
Bolívar, Jorge
Verástegui, Cristina
García Alloza, Mónica
Macías Sánchez, Antonio J.
Hernández Galán, Rosario
Nuñez Abades, Pedro Antonio
2020-05-05T10:14:07Z
2020-05-05T10:14:07Z
2020
Domínguez García, S., Geribaldi Doldán, N., Gómez Oliva, R., Ruiz, F.A., Carrascal Moreno, M.L., Bolívar, J.,...,Nuñez Abades, P.A. (2020). A novel PKC activating molecule promotes neuroblast differentiation and delivery of newborn neurons in brain injuries. Cell Death and Disease, 11 (4), 1-19.
2041-4889
https://hdl.handle.net/11441/96137
10.1038/s41419-020-2453-9
Neural stem cells are activated within neurogenic niches in response to brain injuries. This results in the production of neuroblasts, which unsuccessfully attempt to migrate toward the damaged tissue. Injuries constitute a gliogenic/non-neurogenic niche generated by the presence of anti-neurogenic signals, which impair neuronal differentiation and migration. Kinases of the protein kinase C (PKC) family mediate the release of growth factors that participate in different steps of the neurogenic process, particularly, novel PKC isozymes facilitate the release of the neurogenic growth factor neuregulin. We have demonstrated herein that a plant derived diterpene, (EOF2; CAS number 2230806-06-9), with the capacity to activate PKC facilitates the release of neuregulin 1, and promotes neuroblasts differentiation and survival in cultures of subventricular zone (SVZ) isolated cells in a novel PKC dependent manner. Local infusion of this compound in mechanical cortical injuries induces neuroblast enrichment within the perilesional area, and noninvasive intranasal administration of EOF2 promotes migration of neuroblasts from the SVZ towards the injury, allowing their survival and differentiation into mature neurons, being some of them cholinergic and GABAergic. Our results elucidate the mechanism of EOF2 promoting neurogenesis in injuries and highlight the role of novel PKC isozymes as targets in brain injury regeneration.
Spanish Ministerio de Ciencia, Innovación y Universidades (Grant Numbers RTI2018–099908-B-C21, and RTI2018–099908-BC21)
application/pdf
19 p.
eng
Springer Nature
Cell Death and Disease, 11 (4), 1-19.
RTI2018–099908-BC21
RTI2018–099908-B-C21
http://dx.doi.org/10.1038/s41419-020-2453-9
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
A novel PKC activating molecule promotes neuroblast differentiation and delivery of newborn neurons in brain injuries
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Ciencia, Innovación y Universidades (MICINN). España
Cell Death and Disease
11
4
1
19
https://ror.org/03yxnpp24
oai:idus.us.es:11441/442822024-02-17T17:19:29Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Morado Díaz, Camilo José
Rodríguez Matarredona, Esperanza
Morcuende Fernández, Sara R.
Talaverón Aguilocho, Rocío
Davis López de Carrizosa, María América
Rodríguez de la Cruz, Rosa María
Pastor Loro, Ángel Manuel
2016-08-08T11:01:53Z
2016-08-08T11:01:53Z
2014
Morado Díaz, C.J., Rodríguez Matarredona, E., Morcuende Fernández, S.R., Talaverón Aguilocho, R., Davis López de Carrizosa, M.A., Rodríguez de la Cruz, R.M. y Pastor Loro, Á.M. (2014). Neural Progenitor Cell Implants in the Lesioned Medial Longitudinal Fascicle of Adult Cats Regulate Synaptic Composition and Firing Properties of Abducens Internuclear Neurons. Journal of Neuroscience, 34 (20), 7007-7017.
0270-6474
http://hdl.handle.net/11441/44282
http://dx.doi.org/10.1523/JNEUROSCI.4231-13.2014
https://idus.us.es/xmlui/handle/11441/44282
Transplants of neural progenitor cells (NPCs) into the injured CNS have been proposed as a powerful tool for brain repair, but, to date, few studies on the physiological response of host neurons have been reported. Therefore, we explored the effects of NPC implants on the discharge characteristics and synaptology of axotomized abducens internuclear neurons, which mediate gaze conjugacy for horizontal eye movements. NPCs were isolated from the subventricular zone of neonatal cats and implanted at the site of transection in the medial longitudinal fascicle of adult cats. Abducens internuclear neurons of host animals showed a complete restoration of axotomy-induced alterations in eye position sensitivity, but eye velocity sensitivity was only partially regained. Analysis of the inhibitory and excitatory components of the discharge revealed a normal re-establishment of inhibitory inputs, but only partial re-establishment of excitatory inputs. Moreover, their inhibitory terminal coverage was similar to that in controls, indicating that there was ultimately no loss of inhibitory synaptic inputs. Somatic coverage by synaptophysin-positive contacts, however, showed intermediate values between control animals and animals that had undergone axotomy, likely due to partial loss of excitatory inputs. We also demonstrated that severed axons synaptically contacted NPCs, most of which were VEGF immunopositive, and that abducens internuclear neurons expressed the VEGF receptor Flk1. Together, our results suggest that VEGF neurotrophic support might underlie the increased inhibitory-to-excitatory balance observed in the postimplant cells. The noteworthy improvement of firing properties of injured neurons following NPC implants indicates that these cells might provide a promising therapeutic strategy after neuronal lesions.
application/pdf
eng
Society for Neuroscience
Journal of Neuroscience, 34 (20), 7007-7017.
10.1523/JNEUROSCI.4231-13.2014
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Abducens
Eye movements
Motoneuron
Oculomotor
Plasticity
Neural Progenitor Cell Implants in the Lesioned Medial Longitudinal Fascicle of Adult Cats Regulate Synaptic Composition and Firing Properties of Abducens Internuclear Neurons
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Journal of Neuroscience
34
20
7007
7017
https://ror.org/03yxnpp24
11 p.
oai:idus.us.es:11441/676312024-02-13T22:08:23Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Leiva Mendoza, Andrea
Fuenzalida, Bárbara
Westermeier, Francisco
Toledo, Fernando
Salomón Gallo, Carlos
Gutiérrez, Jaime
Sanhueza, Carlos
Sobrevia Luarte, Luis
2017-12-14T17:54:47Z
2017-12-14T17:54:47Z
2015
Leiva, A., Fuenzalida, B., Westermeier, ., Toledo, F., Salomón Gallo, C., Gutiérrez, J.,...,Sobrevia Luarte, L. (2015). Role for Tetrahydrobiopterin in the Fetoplacental Endothelial Dysfunction in Maternal Supraphysiological Hypercholesterolemia. Oxidative Medicine and Cellular Longevity, 5346327, 1-10.
1942-0900
http://hdl.handle.net/11441/67631
10.1155/2016/5346327
Maternal physiological hypercholesterolemia occurs during pregnancy, ensuring normal fetal development. In some cases, the maternal plasma cholesterol level increases to above this physiological range, leading to maternal supraphysiological hypercholesterolemia (MSPH). This condition results in endothelial dysfunction and atherosclerosis in the fetal and placental vasculature. The fetal and placental endothelial dysfunction is related to alterations in the L-arginine/nitric oxide (NO) pathway and the arginase/urea pathway and results in reduced NO production. The level of tetrahydrobiopterin (BH4), a cofactor for endothelial NO synthase (eNOS), is reduced in nonpregnant women who have hypercholesterolemia, which favors the generation of the superoxide anion rather than NO (from eNOS), causing endothelial dysfunction. However, it is unknown whether MSPH is associated with changes in the level or metabolism of BH4; as a result, eNOS function is not well understood. This review summarizes the available information on the potential link between MSPH and BH4 in causing human fetoplacental vascular endothelial dysfunction, which may be crucial for understanding the deleterious effects of MSPH on fetal growth and development.
application/pdf
eng
Hindawi Publishing Corporation
Oxidative Medicine and Cellular Longevity, 5346327, 1-10.
http://dx.doi.org/10.1155/2016/5346327
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Role for Tetrahydrobiopterin in the Fetoplacental Endothelial Dysfunction in Maternal Supraphysiological Hypercholesterolemia
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Oxidative Medicine and Cellular Longevity
5346327
1
10
https://ror.org/03yxnpp24
10 p.
oai:idus.us.es:11441/1302982024-02-14T13:26:40Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Gómez Oliva, Ricardo
Geribaldi Doldán, Noelia
Domínguez García, Samuel
Carrascal Moreno, María Livia
Verástegui, Cristina
Núñez Abades, Pedro Antonio
Castro, Carmen
2022-03-02T07:55:20Z
2022-03-02T07:55:20Z
2020
Gómez Oliva, R., Geribaldi Doldán, N., Domínguez García, S., Carrascal Moreno, M.L., Verástegui, C., Núñez Abades, P.A. y Castro, C. (2020). Vitamin D deficiency as a potential risk factor for accelerated aging, impaired hippocampal neurogenesis and cognitive decline: a role for Wnt/β-catenin signaling. Aging, 12 (13), 13824-13844.
1945-4589
https://hdl.handle.net/11441/130298
10.18632/aging.103510
Vitamin D is an essential fat-soluble vitamin that participates in several homeostatic functions in mammalian
organisms. Lower levels of vitamin D are produced in the older population, vitamin D deficiency being an
accelerating factor for the progression of the aging process. In this review, we focus on the effect that vitamin D
exerts in the aged brain paying special attention to the neurogenic process. Neurogenesis occurs in the adult
brain in neurogenic regions, such as the dentate gyrus of the hippocampus (DG). This region generates new
neurons that participate in cognitive tasks. The neurogenic rate in the DG is reduced in the aged brain because
of a reduction in the number of neural stem cells (NSC). Homeostatic mechanisms controlled by the Wnt
signaling pathway protect this pool of NSC from being depleted. We discuss in here the crosstalk between Wnt
signaling and vitamin D, and hypothesize that hypovitaminosis might cause failure in the control of the
neurogenic homeostatic mechanisms in the old brain leading to cognitive impairment. Understanding the
relationship between vitamin D, neurogenesis and cognitive performance in the aged brain may facilitate
prevention of cognitive decline and it can open a door into new therapeutic fields by perspectives in the
elderly.
Ministerio de Ciencia, Innovación y Universidades RTI2018-099908-B-C21
FEDER-UCA18-10664
application/pdf
21 p.
eng
Impact Journals LLC
Aging, 12 (13), 13824-13844.
RTI2018-099908-B-C21
FEDER-UCA18-10664
http://dx.doi.org/10.18632/aging.103510
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Vitamin D deficiency as a potential risk factor for accelerated aging, impaired hippocampal neurogenesis and cognitive decline: a role for Wnt/β-catenin signaling
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Ciencia, Innovación y Universidades (MICINN). España
European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
Aging
12
13
13824
13844
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1520732024-02-14T13:32:00Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Peral Rubio, María José
Vázquez Carretero, María Dolores
Ilundáin Larrañeta, María Anunciación Ana
2023-12-01T15:02:19Z
2023-12-01T15:02:19Z
2010
Peral Rubio, M.J., Vázquez Carretero, M.D. y Ilundáin Larrañeta, M.A.A. (2010). Na+/Cl-/creatine transporter activity and expression in rat brain synaptosomes. Neuroscience, 165 (1), 53-60. https://doi.org/10.1016/j.neuroscience.2009.10.001.
0306-4522
1873-7544
https://hdl.handle.net/11441/152073
10.1016/j.neuroscience.2009.10.001
Creatine is involved in brain ATP homeostasis and it may also act as neurotransmitter. Creatine transport was measured in synaptosomes obtained from the diencephalon and telencephalon of suckling and 2 month-old rats. Synaptosomes accumulate [14C]-creatine and this accumulation was Na+- and Cl--dependent and inhibited by high external K+. The latter suggests that the uptake process is electrogenic. The kinetic study revealed a Km for creatine of 8.7 μM. A 100-fold excess of either non-labelled creatine or guanidinopropionic acid abolished NaCl/creatine uptake, whereas GABA uptake was minimally modified, indicating a high substrate specificity of the creatine transporter. The levels of NaCl/creatine transporter (CRT) activity and those of the 4.2 kb CRT transcript (Northern's) were higher in the diencephalon than in the telencephalon, whereas the 2.7 kb transcript levels were similar in both brain regions and lower than those of the 4.2 kb. These observations suggest that the 4.2 kb transcript may code for the functional CRT. CRT activity and mRNA levels were similar in suckling and adult rats. To our knowledge the current results constitute the first description of the presence of a functional CRT in the axon terminal membrane that may serve to recapture the creatine released during the synapsis.
Ministerio de Ciencia y Tecnología 2006-00720
European Regional Development Fund (ERDF) BFU2007- 30688-E/BFI
application/pdf
27 p.
eng
Elsevier
Neuroscience, 165 (1), 53-60.
2006-00720
BFU2007- 30688-E/BFI
https://doi.org/10.1016/j.neuroscience.2009.10.001
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Brain
Creatine transport
CRT
Na+/Cl-/creatine transporter activity and expression in rat brain synaptosomes
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Ciencia y Tecnología (MCYT). España
European Regional Development Fund (ERDF)
Neuroscience
165
1
53
60
https://ror.org/03yxnpp24
oai:idus.us.es:11441/574302019-04-03T05:54:15Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Torres Torrelo, Julio
Torres Ruiz, Blas
Carrascal Moreno, María Livia
2017-04-10T12:33:57Z
2017-04-10T12:33:57Z
2014
Torres Torrelo, J., Torres Ruiz, B. y Carrascal Moreno, M.L. (2014). Modulation of the input–output function by GABAA receptor-mediated currents in rat oculomotor nucleus motoneurons. Journal of Physiology, 592 (Pt 22), 5047-5064.
0022-3751
http://hdl.handle.net/11441/57430
10.1113/jphysiol.2014.276576
The neuronal input–output function depends on recruitment threshold and gain of the firing frequency–current (f–I) relationship. These two parameters are positively correlated in ocular motoneurons (MNs) recorded in alert preparation and inhibitory inputs could contribute to this correlation. Phasic inhibition mediated by γ-amino butyric acid (GABA) occurs when a high concentration of GABA at the synaptic cleft activates postsynaptic GABAA receptors, allowing neuronal information transfer. In some neuronal populations, low concentrations of GABA activate non-synaptic GABAA receptors and generate a tonic inhibition, which modulates cell excitability. This study determined how ambient GABA concentrations modulate the input–output relationship of rat oculomotor nucleus MNs. Superfusion of brain slices with GABA (100 μm) produced a GABAA receptor-mediated current that reduced the input resistance, increased the recruitment threshold and shifted the f–I relationship rightward without any change in gain. These modifications did not depend on MN size. In absence of exogenous GABA, gabazine (20 μm; antagonist of GABAA receptors) abolished spontaneous inhibitory postsynaptic currents and revealed a tonic current in MNs. Gabazine increased input resistance and decreased recruitment threshold mainly in larger MNs. The f–I relationship shifted to the left, without any change in gain. Gabazine effects were chiefly due to MN tonic inhibition because tonic current amplitude was five-fold greater than phasic. This study demonstrates a tonic inhibition in ocular MNs that modulates cell excitability depending on cell size. We suggest that GABAA tonic inhibition acting concurrently with glutamate receptors activation could reproduce the positive covariation between threshold and gain reported in alert preparation.
España, Ministerio de Ciencia y Tecnología BFU 2009-07867
application/pdf
eng
Physiological Society
Journal of Physiology, 592 (Pt 22), 5047-5064.
BFU 2009-07867
http://dx.doi.org/10.1113/jphysiol.2014.276576
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Modulation of the input–output function by GABAA receptor-mediated currents in rat oculomotor nucleus motoneurons
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Ciencia y Tecnología (MCYT). España
Journal of Physiology
592
Pt 22
5047
5064
18 p.
oai:idus.us.es:11441/917762024-02-15T07:41:45Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
García Miranda, Pablo
Morón Civanto, Francisco Jesús
Martínez Olivo, María del Mar
Suárez-Luna, Nela
Ramírez Lorca, Reposo
Lebrato-Hernández, Lucía
Lamas-Pérez, Lucía
Navarro, Gemma
Abril-Jaramillo, Javier
García Sánchez, María Isabel
Casado Chocán, José Luis
Uclés Sánchez, Antonio José
Romera, Mercedes
Echevarría Irusta, Miriam
Díaz Sánchez, María
2020-01-17T08:40:16Z
2020-01-17T08:40:16Z
2019-11-19
García Miranda, P., Morón Civanto, F.J., Martínez Olivo, M.d.M., Suárez-Luna, N., Ramírez Lorca, R., Lebrato-Hernández, L.,...,Díaz Sánchez, M. (2019). Predictive Value of Serum Antibodies and Point Mutations of AQP4, AQP1 and MOG in A Cohort of Spanish Patients with Neuromyelitis Optica Spectrum Disorders. International Journal of Molecular Sciences, 20 (22), 5810.
1422-0067
https://hdl.handle.net/11441/91776
10.3390/ijms20225810
The detection of IgG aquaporin-4 antibodies in the serum of patients with Neuromyelitis optica (NMO) has dramatically improved the diagnosis of this disease and its distinction from multiple sclerosis. Recently, a group of patients have been described who have an NMO spectrum disorder (NMOsd) and who are seronegative for AQP4 antibodies but positive for IgG aquaporin-1 (AQP1) or myelin oligodendrocyte glycoprotein (MOG) antibodies. The purpose of this study was to determine whether AQP1 and MOG could be considered new biomarkers of this disease; and if point mutations in the gDNA of AQP4, AQP1 and MOG genes could be associated with the etiology of NMOsd. We evaluated the diagnostic capability of ELISA and cell-based assays (CBA), and analyzed their reliability, specificity, and sensitivity in detecting antibodies against these three proteins. The results showed that both assays can recognize these antigen proteins under appropriate conditions, but only anti-AQP4 antibodies, and not AQP1 or MOG, appears to be a clear biomarker for NMOsd. CBA is the best method for detecting these antibodies; and serum levels of AQP4 antibodies do not correlate with the progression of this disease. So far, the sequencing analysis has not revealed a genetic basis for the etiology of NMOsd, but a more extensive analysis is required before definitive conclusions can be drawn.
Ministerio de Economía y Competitividad
FEDER (Grants PI16/01249 y PI16/00493)
application/pdf
eng
MDPI
International Journal of Molecular Sciences, 20 (22), 5810.
PI16/01249
PI16/00493
http://dx.doi.org/10.3390/ijms20225810
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
demyelinating disease
NMOsd
AQPs
MOG
gene sequencing
immunohistochemistry
Predictive Value of Serum Antibodies and Point Mutations of AQP4, AQP1 and MOG in A Cohort of Spanish Patients with Neuromyelitis Optica Spectrum Disorders
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. Departamento de Fisiología Médica y Biofísica
International Journal of Molecular Sciences
20
22
5810
https://ror.org/03yxnpp24
17 p.
oai:idus.us.es:11441/1292852024-02-15T07:40:37Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Pérez García, Patricia
Pardillo Díaz, Ricardo
Geribaldi Doldán, Noelia
Gómez Oliva, Ricardo
Domínguez García, Samuel
Castro, Carmen
Núñez Abades, Pedro Antonio
Carrascal Moreno, María Livia
2022-01-26T18:22:38Z
2022-01-26T18:22:38Z
2021
Pérez García, P., Pardillo Díaz, R., Geribaldi Doldán, N., Gómez Oliva, R., Domínguez García, S., Castro, C.,...,Carrascal Moreno, M.L. (2021). Refinement of Active and Passive Membrane Properties of Layer V Pyramidal Neurons in Rat Primary Motor Cortex During Postnatal Development. Frontiers in Molecular Neuroscience, 14, 754393.
1662-5099
https://hdl.handle.net/11441/129285
10.3389/fnmol.2021.754393
Achieving the distinctive complex behaviors of adult mammals requires the development of a great variety of specialized neural circuits. Although the development of these circuits begins during the embryonic stage, they remain immature at birth, requiring a postnatal maturation process to achieve these complex tasks. Understanding how the neuronal membrane properties and circuits change during development is the first step to understand their transition into efficient ones. Thus, using whole cell patch clamp recordings, we have studied the changes in the electrophysiological properties of layer V pyramidal neurons of the rat primary motor cortex during postnatal development. Among all the parameters studied, only the voltage threshold was established at birth and, although some of the changes occurred mainly during the second postnatal week, other properties such as membrane potential, capacitance, duration of the post-hyperpolarization phase or the maximum firing rate were not defined until the beginning of adulthood. Those modifications lead to a decrease in neuronal excitability and to an increase in the working range in young adult neurons, allowing more sensitive and accurate responses. This maturation process, that involves an increase in neuronal size and changes in ionic conductances, seems to be influenced by the neuronal type and by the task that neurons perform as inferred from the comparison with other pyramidal and motor neuron populations.
Ministerio de Ciencia, Innovación y Universidades RTI2018-099908-BC21
Junta de Andalucía FEDER-UCA18-106647
application/pdf
14 p.
eng
Frontiers Media S.A.
Frontiers in Molecular Neuroscience, 14, 754393.
RTI2018-099908-BC21
FEDER-UCA18-106647
https://doi.org/10.3389/fnmol.2021.754393
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Development
Membrane properties
Motor cortex
Motor neurons
Patch clamp
Pyramidal neurons
Refinement of Active and Passive Membrane Properties of Layer V Pyramidal Neurons in Rat Primary Motor Cortex During Postnatal Development
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Frontiers in Molecular Neuroscience
14
754393
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1472062024-02-13T09:18:04Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Caravaca Rodríguez, Daniel
Gaytán Guía, Susana Pilar
Suaning, G.J.
Barriga Rivera, Alejandro
2023-06-14T11:05:08Z
2023-06-14T11:05:08Z
2022
Caravaca Rodríguez, D., Gaytán Guía, S.P., Suaning, G.J. y Barriga Rivera, A. (2022). Implications of Neural Plasticity in Retinal Prosthesis.. Investigative Ophthalmology & Visual Science (IOVS), 63 (11), 11. https://doi.org/10.1167/iovs.63.11.11.
0146-0404
1552-5783
https://hdl.handle.net/11441/147206
10.1167/iovs.63.11.11
Retinal degenerative diseases such as retinitis pigmentosa cause a progressive loss of
photoreceptors that eventually prevents the affected person from perceiving visual sensations. The absence of a visual input produces a neural rewiring cascade that propagates
along the visual system. This remodeling occurs first within the retina. Then, subsequent
neuroplastic changes take place at higher visual centers in the brain, produced by either
the abnormal neural encoding of the visual inputs delivered by the diseased retina or
as the result of an adaptation to visual deprivation. While retinal implants can activate
the surviving retinal neurons by delivering electric current, the unselective activation
patterns of the different neural populations that exist in the retinal layers differ substantially from those in physiologic vision. Therefore, artificially induced neural patterns are
being delivered to a brain that has already undergone important neural reconnections.
Whether or not the modulation of this neural rewiring can improve the performance
for retinal prostheses remains a critical question whose answer may be the enabler of
improved functional artificial vision and more personalized neurorehabilitation strategies.
Ministerio de Ciencia e Innovación y Agencia Estatal de Investigación, de España y fondos FEDER (MICIN/ AEI) RTI2018-094465-J-I00
application/pdf
18 p.
eng
Association for Research in Vision and Ophthalmology
Investigative Ophthalmology & Visual Science (IOVS), 63 (11), 11.
RTI2018-094465-J-I00
https://doi.org/10.1167/iovs.63.11.11
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
visual prosthesis
neural plasticity
retinal implant
Implications of Neural Plasticity in Retinal Prosthesis.
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. Departamento de Física Aplicada III
Ministerio de Ciencia e Innovación (MICIN). España
Agencia Estatal de Investigación. España
European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
Investigative Ophthalmology & Visual Science (IOVS)
63
11
11
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1516852024-03-15T10:47:05Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Martín Calvo, Paula
García Hernández, Rosendo Miguel
Rodríguez de la Cruz, Rosa María
Pastor Loro, Ángel Manuel
2023-11-27T16:15:40Z
2023-11-27T16:15:40Z
2022
Martín Calvo, P., García Hernández, R.M., Rodríguez de la Cruz, R.M. y Pastor Loro, Á.M. (2022). VEGF is an essential retrograde trophic factor for motoneurons. Proceedings of the National Academy of Sciences of the United States of America, 119 (26), e2202912119. https://doi.org/10.1073/pnas.2202912119.
0027-8424
1091-6490
https://hdl.handle.net/11441/151685
10.1073/pnas.2202912119
VEGF was initially discovered due to its angiogenic activity and therefore named "vascular endothelial growth factor."However, its more recently discovered neurotrophic activity may be evolutionarily more ancient. Our previous work showed that all the changes produced by axotomy on the firing activity and synaptic inputs of abducens motoneurons were completely restored after VEGF administration. Therefore, we hypothesized that the lack of VEGF delivered by retrograde transport from the periphery should also affect the physiology of otherwise intact abducens motoneurons. For VEGF retrograde blockade, we chronically applied a neutralizing VEGF antibody to the lateral rectus muscle. Recordings of extracellular single-unit activity and eye movements were made in alert cats before and after the application of the neutralizing antibody. Our data revealed that intact, noninjured abducens motoneurons retrogradely deprived of VEGF exhibited noticeable changes in their firing pattern. There is a general decrease in firing rate and a significant reduction in eye position and eye velocity sensitivity (i.e., a decrease in the tonic and phasic components of their discharge, respectively). Moreover, by means of confocal immunocytochemistry, motoneurons under VEGF blockade showed a marked reduction in the density of afferent synaptic terminals contacting with their cell bodies. Altogether, the present findings demonstrate that the lack of retrogradely delivered VEGF renders abducens motoneurons into an axotomy-like state. This indicates that VEGF is an essential retrograde factor for motoneuronal synaptic drive and discharge activity.
Ministerio de Ciencia e Innovación PGC2018-094654-B-100
Junta de Andalucía P20_00529, BIO-297
Premio Anual Publicación Científica Destacada de la US. Facultad de Biología
application/pdf
42 p.
eng
National Academy of Sciences
Proceedings of the National Academy of Sciences of the United States of America, 119 (26), e2202912119.
PGC2018-094654-B-100
P20_00529
BIO-297
https://doi.org/10.1073/pnas.2202912119
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Abducens
Axotomy
Neurotrophic factors
Oculomotor
Synaptic stripping
VEGF is an essential retrograde trophic factor for motoneurons
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Ciencia e Innovación (MICIN). España
Junta de Andalucía
Proceedings of the National Academy of Sciences of the United States of America
119
26
e2202912119
https://ror.org/03yxnpp24
oai:idus.us.es:11441/824512024-02-13T09:42:47Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Benítez Temiño, Beatriz
Morado Díaz, Camilo José
Davis López de Carrizosa, María América
Rodríguez de la Cruz, Rosa María
Pastor Loro, Ángel Manuel
G. Hernández, Rosendo
2019-02-04T12:22:30Z
2019-02-04T12:22:30Z
2018
Benítez Temiño, B., Morado Díaz, C.J., Davis López de Carrizosa, M.A., Rodríguez de la Cruz, R.M., Pastor Loro, Á.M. y G. Hernández, R. (2018). Effects of Selective Deafferentation on the Discharge Characteristics of Medial Rectus Motoneurons. journal of neursocience, 37 (38), 9172-9188.
1529-2401
https://hdl.handle.net/11441/82451
10.1523/JNEUROSCI.1391-17.2017
Medial rectus motoneurons receive two main pontine inputs: abducens internuclear neurons, whose axons course through the medial longitudinal fasciculus (MLF), and neurons in the lateral vestibular nucleus, whose axons project through the ascending tract of Deiters (ATD). Abducens internuclear neurons are responsible for conjugate gaze in the horizontal plane, whereas ATD neurons provide medial rectus motoneurons with a vestibular input comprising mainly head velocity. To reveal the relative contribution of each input to the oculomotor physiology, single-unit recordings from medial rectus motoneurons were obtained in the control situation and after selective deafferentation from cats with unilateral transection of either the MLF or the ATD. Both MLF and ATD transection produced similar short-term alterations in medial rectus motoneuron firing pattern, which were more drastic in MLF of animals. However, long-term recordings revealed important differences between the two types of lesion. Thus, while the effects of the MLF section were permanent, 2 months after ATD lesioning all motoneuronal firing parameters were similar to the control. These findings indicated a more relevant role of the MLF pathway in driving motoneuronal firing and evidenced compensatory mechanisms following the ATD lesion. Confocal immunocytochemistry revealed that MLF transection produced also a higher loss of synaptic boutons, mainly at the dendritic level. Moreover, 2 months after ATD transection, we observed an increase in synaptic coverage around motoneuron cell bodies compared with short-term data, which is indicative of a synaptogenic compensatory mechanism of the abducens internuclear pathway that could lead to the observed firing and morphological recovery.
SIGNIFICANCE STATEMENT Eye movements rely on multiple neuronal circuits for appropriate performance. The abducens internuclear pathway through the medial longitudinal fascicle (MLF) and the vestibular neurons through the ascending tract of Deiters (ATD) are a dual system that supports the firing of medial rectus motoneurons. We report the effect of sectioning the MLF or the ATD pathway on the firing of medial rectus motoneurons, as well as the plastic mechanisms by which one input compensates for the lack of the other. This work shows that while the effects of MLF transection are permanent, the ATD section produces transitory effects. A mechanism based on axonal sprouting and occupancy of the vacant synaptic space due to deafferentation is the base for the mechanism of compensation on the medial rectus motoneuron.
application/pdf
eng
Society for Neuroscience
journal of neursocience, 37 (38), 9172-9188.
https://doi.org/10.1523/JNEUROSCI.1391-17.2017
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
lesion-induced plasticity
motoneuron
oculomotor system
Effects of Selective Deafferentation on the Discharge Characteristics of Medial Rectus Motoneurons
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
journal of neursocience
37
38
9172
9188
https://ror.org/03yxnpp24
8 p.
oai:idus.us.es:11441/453932024-02-14T19:16:47Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Ojeda Murillo, María Luisa
Nogales Bueno, Fátima
Carreras Sánchez, Olimpia
2016-09-26T10:04:05Z
2016-09-26T10:04:05Z
2014
Ojeda Murillo, M.L., Nogales Bueno, F. y Carreras Sánchez, O. (2014). “A Concurso un congreso”. Innovación docente en la asignatura “Fisiología de la digestión”. Ars Pharmaceutica, 55 (suppl. 2), 41-41.
0004-2927
http://hdl.handle.net/11441/45393
https://idus.us.es/xmlui/handle/11441/45393
application/pdf
spa
Universidad de Granada
Ars Pharmaceutica, 55 (suppl. 2), 41-41.
http://farmacia.ugr.es/ars/ars_web/controldescargas.php?875
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
“A Concurso un congreso”. Innovación docente en la asignatura “Fisiología de la digestión”
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Ars Pharmaceutica
55
suppl. 2
41
41
https://ror.org/03yxnpp24
1 p.
oai:idus.us.es:11441/1516922024-02-12T21:43:46Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Martín Calvo, Paula
Rodríguez de la Cruz, Rosa María
Pastor Loro, Ángel Manuel
2023-11-27T19:23:23Z
2023-11-27T19:23:23Z
2018
Martín Calvo, P., Rodríguez de la Cruz, R.M. y Pastor Loro, Á.M. (2018). Synaptic loss and firing alterations in Axotomized Motoneurons are restored by vascular endothelial growth factor (VEGF) and VEGF-B. Experimental Neurology, 304, 67-81. https://doi.org/10.1016/j.expneurol.2018.03.004.
0014-4886
1090-2430
https://hdl.handle.net/11441/151692
10.1016/j.expneurol.2018.03.004
Vascular endothelial growth factor (VEGF), also known as VEGF-A, was discovered due to its vasculogenic and angiogenic activity, but a neuroprotective role for VEGF was later proven for lesions and disorders. In different models of motoneuronal degeneration, VEGF administration leads to a significant reduction of motoneuronal death. However, there is no information about the physiological state of spared motoneurons. We examined the trophic role of VEGF on axotomized motoneurons with recordings in alert animals using the oculomotor system as the experimental model, complemented with a synaptic study at the confocal microscopy level. Axotomy leads to drastic alterations in the discharge characteristics of abducens motoneurons, as well as to a substantial loss of their synaptic inputs. Retrograde delivery of VEGF completely restored the discharge activity and synaptically-driven signals in injured motoneurons, as demonstrated by correlating motoneuronal firing rate with motor performance. Moreover, VEGF-treated motoneurons recovered a normal density of synaptic boutons around motoneuronal somata and in the neuropil, in contrast to the low levels of synaptic terminals found after axotomy. VEGF also reduced the astrogliosis induced by axotomy in the abducens nucleus to control values. The administration of VEGF-B produced results similar to those of VEGF. This is the first work demonstrating that VEGF and VEGF-B restore the normal operating mode and synaptic inputs on injured motoneurons. Altogether these data indicate that these molecules are relevant synaptotrophic factors for motoneurons and support their clinical potential for the treatment of motoneuronal disorders.
Ministerio de Economía y Competitividad BFU2015-64515-P
application/pdf
58 p.
eng
Elsevier
Experimental Neurology, 304, 67-81.
BFU2015-64515-P
https://doi.org/10.1016/j.expneurol.2018.03.004
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Abducens nucleus
Astrogliosis
Axotomy
Chronic single-unit recording
Lesion-induced plasticity
Neurotrophic factors
Oculomotor system
Synaptic stripping
Synaptic loss and firing alterations in Axotomized Motoneurons are restored by vascular endothelial growth factor (VEGF) and VEGF-B
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Fisiología
Ministerio de Economía y Competitividad (MINECO). España
Experimental Neurology
304
67
81
https://ror.org/03yxnpp24
oai:idus.us.es:11441/442832024-02-14T19:08:44Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Morcuende Fernández, Sara R.
Delgado García, José María
Ugolini, Gabriella
2016-08-08T11:02:21Z
2016-08-08T11:02:21Z
2002
Morcuende Fernández, S.R., Delgado García, J.M. y Ugolini, G. (2002). Neuronal Premotor Networks Involved in Eyelid Responses: Retrograde Transneuronal Tracing with Rabies Virus from the Orbicularis Oculi Muscle in the Rat. Journal of Neuroscience, 22 (20), 8808-8818.
0270-6474
http://hdl.handle.net/11441/44283
https://idus.us.es/xmlui/handle/11441/44283
Retrograde transneuronal tracing with rabies virus from the right orbicularis oculi muscle was used to identify neural networks underlying spontaneous, reflex, and learned blinks. The kinetics of viral transfer was studied at sequential 12 hr intervals between 3 and 5 d after inoculation. Rabies virus immunolabeling was combined with the immunohistochemical detection of choline acetyltransferase expression in brainstem motoneurons or Fluoro-Ruby injections in the rubrospinal tract. Virus uptake involved exclusively orbicularis oculi motoneurons in the dorsolateral division of the facial nucleus. At 3-3.5 d, transneuronal transfer involved premotor interneurons of trigeminal, auditory, and vestibular reflex pathways (in medullary and pontine reticular formation, trigeminal nuclei, periolivary and ventral cochlear nuclei, and medial vestibular nuclei), motor pathways (dorsolateral quadrant of contralateral red nucleus and pararubral area), deep cerebellar nuclei (lateral portion of interpositus nucleus and dorsolateral hump ipsilaterally), limbic relays (parabrachial and Kölliker-Fuse nuclei), and oculomotor structures involved in eye-eyelid coordination (oculomotor nucleus, supraoculomotor area, and interstitial nucleus of Cajal). At 4 d, higher order neurons were revealed in trigeminal, auditory, vestibular, and deep cerebellar nuclei (medial, interpositus, and lateral), oculomotor and visual-related structures (Darkschewitsch, nucleus of the posterior commissure, deep layers of superior colliculus, and pretectal area), lateral hypothalamus, and cerebral cortex (particularly in parietal areas). At 4.5 and 5 d the labeling of higher order neurons occurred in hypothalamus, cerebral cortex, and blink-related areas of cerebellar cortex. These results provide a comprehensive picture of the premotor networks mediating reflex, voluntary, and limbic-related eyelid responses and highlight potential sites of motor learning in eyelid classical conditioning.
application/pdf
eng
Society for Neuroscience
Journal of Neuroscience, 22 (20), 8808-8818.
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
Cerebellum
Eyelid responses
Parietal cortex
Rabies virus
Red nucleus
Reflex blinks
Reticular formation
Neuronal Premotor Networks Involved in Eyelid Responses: Retrograde Transneuronal Tracing with Rabies Virus from the Orbicularis Oculi Muscle in the Rat
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Journal of Neuroscience
22
20
8808
8818
https://ror.org/03yxnpp24
11 p.
oai:idus.us.es:11441/1524552024-02-14T09:05:22Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Ojeda Murillo, María Luisa
Nogales Bueno, Fátima
Serrano, Alejandra
Murillo Taravillo, María Luisa
Carreras Sánchez, Olimpia
2023-12-13T18:35:39Z
2023-12-13T18:35:39Z
2020-05
Ojeda Murillo, M.L., Nogales Bueno, F., Serrano, A., Murillo Taravillo, M.L. y Carreras Sánchez, O. (2020). Selenoproteins and renal programming in metabolic syndrome-exposed rat offspring. Food and Function, 11 (5), 3904-3915. https://doi.org/10.1039/d0fo00264j.
2042-6496
2042-650X
https://hdl.handle.net/11441/152455
10.1039/d0fo00264j
Maternal metabolic syndrome (MS) during gestation and lactation leads to several cardiometabolic changes related to selenium (Se) status and selenoprotein expression in offspring. However, little is known about kidney programming and antioxidant selenoprotein status in MS pups. To gain more knowledge on this subject, two experimental groups of dam rats were used: Control (Se: 0.1 ppm) and MS (fructose 65% and Se: 0.1 ppm). At the end of lactation, Se deposits in kidneys, selenoprotein expression (GPx1, GPx3, GPx4 and selenoprotein P), oxidative balance and AMP-activated protein kinase (AMPK) and activated transcriptional factor NF-κB expression were measured. Kidney functional parameters, albuminuria, creatinine clearance, aldosteronemia, and water and electrolyte balance, were also evaluated. One week later systolic blood pressure was measured. Lipid peroxidation takes place in the kidneys of MS pups and Se, selenoproteins and NF-κB expression increased, while AMPK activation decreased. MS pups have albuminuria and low creatinine clearance which implies glomerular renal impairment with protein loss. They also present hypernatremia and hyperaldosteronemia, together with a high renal Na+ reabsorption, leading to a hypertensive status, which was detected in these animals one week later. Since these alterations seem to be related, at least in part, to oxidative stress, the increase in Se and selenoproteins found in the kidneys of these pups seems to be beneficial, avoiding a higher lipid oxidation. However, in order to analyze the possible global beneficial role of Se in kidneys during MS exposure, more data are necessary to document the relationships between GPx4 and NF-κB, and SelP and AMPK in kidneys.
Junta de Andalucía CTS-193
application/pdf
28 p.
eng
Royal Society of Chemistry
Food and Function, 11 (5), 3904-3915.
CTS-193
https://doi.org/10.1039/d0fo00264j
Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
Selenoproteins and renal programming in metabolic syndrome-exposed rat offspring
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
Universidad de Sevilla. Departamento de Fisiología
Junta de Andalucía
Food and Function
11
5
3904
3915
https://ror.org/03yxnpp24
oai:idus.us.es:11441/1052422024-02-14T11:14:33Zcom_11441_10873com_11441_10802com_11441_10690col_11441_10874
Silva Hucha, Silvia
Pastor Loro, Ángel Manuel
Morcuende Fernández, Sara R.
2021-02-22T12:15:55Z
2021-02-22T12:15:55Z
2021-01
Silva Hucha, S., Pastor Loro, Á.M. y Morcuende Fernández, S.R. (2021). Neuroprotective Effect of Vascular Endothelial Growth Factor on Motoneurons of the Oculomotor System. International Journal of Molecular Sciences, 22 (2), 814.
1422-0067
1661-6596
https://hdl.handle.net/11441/105242
10.3390/ijms22020814
Vascular endothelial growth factor (VEGF) was initially characterized as a potent angiogenic factor based on its activity on the vascular system. However, it is now well established
that VEGF also plays a crucial role as a neuroprotective factor in the nervous system. A deficit
of VEGF has been related to motoneuronal degeneration, such as that occurring in amyotrophic
lateral sclerosis (ALS). Strikingly, motoneurons of the oculomotor system show lesser vulnerability to
neurodegeneration in ALS compared to other motoneurons. These motoneurons presented higher
amounts of VEGF and its receptor Flk-1 than other brainstem pools. That higher VEGF level could
be due to an enhanced retrograde input from their target muscles, but it can also be produced by
the motoneurons themselves and act in an autocrine way. By contrast, VEGF’s paracrine supply
from the vicinity cells, such as glial cells, seems to represent a minor source of VEGF for brainstem
motoneurons. In addition, ocular motoneurons experiment an increase in VEGF and Flk-1 level in
response to axotomy, not observed in facial or hypoglossal motoneurons. Therefore, in this review,
we summarize the differences in VEGF availability that could contribute to the higher resistance of
extraocular motoneurons to injury and neurodegenerative diseases.
Ministerio de Ciencia e Innovación de España (MCI), Agencia Estatal de Investigación de España (AEI) y Fondo Europeo de Desarrollo Regional (FEDER)-BFU2015-64515-P y PGC2018-094654-B-100
Junta de Andalucía-BIO-297
application/pdf
20 p.
eng
MDPI
International Journal of Molecular Sciences, 22 (2), 814.
BFU2015-64515-P
PGC2018-094654-B-100
BIO-297
https://doi.org/10.3390/ijms22020814
Attribution-NonCommercial-NoDerivatives 4.0 Internacional
http://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
VEGF
oculomotor system
trophic factors
motoneurons
neurodegeneration
axotomy
amyotrophic lateral sclerosis
Neuroprotective Effect of Vascular Endothelial Growth Factor on Motoneurons of the Oculomotor System
info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Universidad de Sevilla. Departamento de Fisiología
Universidad de Sevilla. BIO297: Laboratorio de Fisiología y Plasticidad Neuronal
Ministerio de Ciencia e Innovación (MICIN). España
Agencia Estatal de Investigación. España
European Commission (EC). Fondo Europeo de Desarrollo Regional (FEDER)
Junta de Andalucía
International Journal of Molecular Sciences
22
2
814
https://ror.org/03yxnpp24
dim///col_11441_10874/100