dc.creator | Díaz Moreno, Irene | es |
dc.creator | García Mayoral, María Flor | es |
dc.creator | Hollingworth, David | es |
dc.creator | Ramos, Andrés | es |
dc.date.accessioned | 2019-03-26T12:17:34Z | |
dc.date.available | 2019-03-26T12:17:34Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | Díaz Moreno, I., García Mayoral, M.F., Hollingworth, D. y Ramos, A. (2008). The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets. Nucleic Acids Research, 36 (16), 5290-5296. | |
dc.identifier.issn | 0305-1048 (impreso) | es |
dc.identifier.issn | 1362-4962 (electrónico) | es |
dc.identifier.uri | https://hdl.handle.net/11441/84709 | |
dc.description.abstract | K-homology (KH) splicing regulator protein (KSRP) is a multi-domain RNA-binding protein that regulates different steps of mRNA metabolism, from mRNA splicing to mRNA decay, interacting with a broad range of RNA sequences. To understand how KSRP recognizes its different RNA targets it is necessary to define the general rules of KSRP-RNA interaction. We describe here a complete scaffold-independent analysis of the RNA-binding potential of the four KH domains of KSRP. The analysis shows that KH3 binds to the RNA with a significantly higher affinity than the other domains and recognizes specifically a G-rich target. It also demonstrates that the other KH domains of KSRP display different sequence preferences explaining the broad range of targets recognized by the protein. Further, KSRP shows a strong negative selectivity for sequences containing several adjacent Cytosines limiting the target choice of KSRP within single-stranded RNA regions. The in-depth analysis of the RNA-binding potential of the KH domains of KSRP provides us with an understanding of the role of low sequence specificity domains in RNA recognition by multi-domain RNA-binding proteins. | es |
dc.description.sponsorship | Wellcome Trust Grant WT082088MA | es |
dc.description.sponsorship | EMBO fellowship 240-2005 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Oxford University Press | es |
dc.relation.ispartof | Nucleic Acids Research, 36 (16), 5290-5296. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | The sequence selectivity of KSRP explains its flexibility in the recognition of the RNA targets | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.contributor.affiliation | Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular | es |
dc.relation.projectID | WT082088MA | es |
dc.relation.projectID | 240-2005 | es |
dc.relation.publisherversion | http://dx.doi.org/10.1093/nar/gkn509 | es |
dc.identifier.doi | 10.1093/nar/gkn509 | es |
idus.format.extent | 7 p. | es |
dc.journaltitle | Nucleic Acids Research | es |
dc.publication.volumen | 36 | es |
dc.publication.issue | 16 | es |
dc.publication.initialPage | 5290 | es |
dc.publication.endPage | 5296 | es |