Article
Megahertz serial crystallography
Author/s | Wiedorn, Max O.
Oberthür, Dominik Bean, Richard Cruz-Mazo, Francisco Gañán-Calvo, Alfonso M. |
Department | Universidad de Sevilla. Departamento de Ingeniería Aeroespacial y Mecánica de Fluidos |
Publication Date | 2018-10 |
Deposit Date | 2018-11-12 |
Published in |
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Abstract | The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. ... The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previously possible. However, to date, it has been unclear whether it would indeed be possible to measure high-quality diffraction data at megahertz pulse repetition rates. Here, we show that high-quality structures can indeed be obtained using currently available operating conditions at the European XFEL. We present two complete data sets, one from the well-known model system lysozyme and the other from a so far unknown complex of a β-lactamase from K. pneumoniae involved in antibiotic resistance. This result opens up megahertz serial femtosecond crystallography (SFX) as a tool for reliable structure determination, substrate screening and the efficient measurement of the evolution and dynamics of molecular structures using megahertz repetition rate pulses available at this new class of X-ray laser source. |
Citation | Wiedorn, M.O.(et al.) (2018). Megahertz serial crystallography. Nature Communications, 9 (1), Article number 4025-. |
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