A putative RNA binding protein from Plasmodium vivax apicoplast
|Author/s||García Mauriño, Sofía M.
Díaz Quintana, Antonio Jesús
Rivero Rodríguez, Francisco
Cruz Gallardo, Isabel
Hernández Vellisca, Marian
Díaz Moreno, Irene
|Department||Universidad de Sevilla. Departamento de Bioquímica Vegetal y Biología Molecular|
|Abstract||Malaria is caused by Apicomplexa protozoans from the Plasmodium genus entering the bloodstream of humans and animals through the bite of the female mosquitoes. The annotation of the Plasmodium vivax genome revealed a ...
Malaria is caused by Apicomplexa protozoans from the Plasmodium genus entering the bloodstream of humans and animals through the bite of the female mosquitoes. The annotation of the Plasmodium vivax genome revealed a putative RNA binding protein (apiRBP) that was predicted to be trafficked into the apicoplast, a plastid organelle unique to Apicomplexa protozoans. Although a 3D structural model of the apiRBP corresponds to a noncanonical RNA recognition motif with an additional C‐terminal α‐helix (α3), preliminary protein production trials were nevertheless unsuccessful. Theoretical solvation analysis of the apiRBP model highlighted an exposed hydrophobic region clustering α3. Hence, we used a C‐terminal GFP‐fused chimera to stabilize the highly insoluble apiRBP and determined its ability to bind U‐rich stretches of RNA. The affinity of apiRBP toward such RNAs is highly dependent on ionic strength, suggesting that the apiRBP–RNA complex is driven by electrostatic interactions. Altogether, apiRBP represents an attractive tool for apicoplast transcriptional studies and for antimalarial drug design.
|Funding agencies||Junta de Andalucía
Universidad de Sevilla
|Citation||García Mauriño, S.M., Díaz Quintana, A., Rivero Rodríguez, F., Cruz Gallardo, I., Grüttner, C., Hernández Vellisca, M. y Díaz Moreno, I. (2018). A putative RNA binding protein from Plasmodium vivax apicoplast. FEBS Open Bio, 8 (2), 177-188.|