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dc.creatorGalindo Moreno, Maríaes
dc.creatorGiráldez Macías, Servandoes
dc.creatorSáez, Carmenes
dc.creatorJapón Rodríguez, Miguel Ángeles
dc.creatorTortolero García, María Doloreses
dc.creatorRomero Portillo, Franciscoes
dc.date.accessioned2017-11-17T17:03:17Z
dc.date.available2017-11-17T17:03:17Z
dc.date.issued2017
dc.identifier.citationGalindo Moreno, M., Giráldez Macías, S., Sáez, C., Japón Rodríguez, M.Á., Tortolero García, M.D. y Romero Portillo, F. (2017). Both p62/SQSTM1-HDAC6-dependent autophagy and the aggresome pathway mediate CDK1 degradation in human breast cancer. Scientific Reports, 7 (10078), 1-10.
dc.identifier.issn2045-2322es
dc.identifier.urihttp://hdl.handle.net/11441/66242
dc.description.abstractCyclin-dependent kinase 1 (CDK1) is the central mammalian regulator of cell proliferation and a promising therapeutic target for breast cancer. In fact, CDK1 inhibition downregulates survival and induces apoptosis. Due to its essential role, CDK1 expression and activity are strictly controlled at various levels. We previously described that CDK1 stability is also regulated and that SCF(βTrCP) ubiquitinates CDK1, which is degraded via the lysosomal pathway. In addition, in breast tumors from patients, we found a negative correlation between CDK1 accumulation and βTrCP levels, and a positive correlation with the degree of tumor malignancy. This prompted us to study the molecular mechanism involved in CDK1 clearance. In this report, we determine that both chemotherapeutic agents and proteolytic stress induce CDK1 degradation in human breast cancer MCF7 cells through p62/HDAC6-mediated selective autophagy. On the one hand, CDK1 binds to p62/SQSTM1-LC3 and, on the other hand, it interacts with HDAC6. Both complexes are dependent on the presence of an intact βTrCP-binding motif on CDK1. Furthermore, we also show that CDK1 is recruited to aggresomes in response to proteasome inhibition for an extended period. We propose CDK1 clearance as a potential predictive biomarker of antitumor treatment efficacy.es
dc.formatapplication/pdfes
dc.language.isoenges
dc.publisherNature Publishing Groupes
dc.relation.ispartofScientific Reports, 7 (10078), 1-10.
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleBoth p62/SQSTM1-HDAC6-dependent autophagy and the aggresome pathway mediate CDK1 degradation in human breast canceres
dc.typeinfo:eu-repo/semantics/articlees
dc.type.versioninfo:eu-repo/semantics/publishedVersiones
dc.rights.accessrightsinfo:eu-repo/semantics/openAccesses
dc.contributor.affiliationUniversidad de Sevilla. Departamento de Microbiologíaes
dc.relation.publisherversionhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5577189/pdf/41598_2017_Article_10506.pdfes
dc.identifier.doi10.1038/s41598-017-10506-8es
idus.format.extent11 p.es
dc.journaltitleScientific Reportses
dc.publication.volumen7es
dc.publication.issue10078es
dc.publication.initialPage1es
dc.publication.endPage10es

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