dc.creator | Cañete, Ana | es |
dc.creator | Carmona, Rita | es |
dc.creator | Ariza, Laura | es |
dc.creator | Sánchez, Mª José | es |
dc.creator | Rojas, Anabel | es |
dc.creator | Muñoz Chápuli, Ramón | es |
dc.date.accessioned | 2017-08-21T11:35:55Z | |
dc.date.available | 2017-08-21T11:35:55Z | |
dc.date.issued | 2017 | |
dc.identifier.citation | Cañete, A., Carmona, R., Ariza, L., Sánchez, M.J., Rojas, A. y Muñoz Chápuli, R. (2017). A population of hematopoietic stem cells derives from GATA4-expressing progenitors located in the placenta and lateral mesoderm of mice. Haematologica, 102 (4), 647-655. | |
dc.identifier.issn | 0390-6078 | es |
dc.identifier.issn | 1592-8721 electrónico | es |
dc.identifier.uri | http://hdl.handle.net/11441/63935 | |
dc.description.abstract | GATA transcription factors are expressed in the mesoderm and
endoderm during development. GATA1-3, but not GATA4, are
critically involved in hematopoiesis. An enhancer (G2) of the
mouse Gata4 gene directs its expression throughout the lateral mesoderm
and the allantois, beginning at embryonic day 7.5, becoming
restricted to the septum transversum by embryonic day 10.5, and disappearing
by midgestation. We have studied the developmental fate of the
G2-Gata4 cell lineage using a G2-Gata4Cre;R26REYFP mouse line. We found
a substantial number of YFP+ hematopoietic cells of lymphoid, myeloid
and erythroid lineages in embryos. Fetal CD41+
/cKit+
/CD34+ and
Lin–
/cKit+
/CD31+ YFP+ hematopoietic progenitors were much more
abundant in the placenta than in the aorta-gonad-mesonephros area.
They were clonogenic in the MethoCult assay and fully reconstituted
hematopoiesis in myeloablated mice. YFP+ cells represented about 20%
of the hematopoietic system of adult mice. Adult YFP+ hematopoietic
stem cells constituted a long-term repopulating, transplantable population.
Thus, a lineage of adult hematopoietic stem cells is characterized by
the expression of GATA4 in their embryonic progenitors and probably
by its extraembryonic (placental) origin, although GATA4 appeared not
to be required for hematopoietic stem cell differentiation. Both lineages
basically showed similar physiological behavior in normal mice, but clinically
relevant properties of this particular hematopoietic stem cell population
should be checked in physiopathological conditions. | es |
dc.description.sponsorship | España Ministerio de Ecomomía BFU2014-52299-P | es |
dc.description.sponsorship | España Instituto de Salud Carlos III RD12/0019-0022 | es |
dc.format | application/pdf | es |
dc.language.iso | eng | es |
dc.publisher | Ferrata Storti Foundation | es |
dc.relation.ispartof | Haematologica, 102 (4), 647-655. | |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.title | A population of hematopoietic stem cells derives from GATA4-expressing progenitors located in the placenta and lateral mesoderm of mice | es |
dc.type | info:eu-repo/semantics/article | es |
dcterms.identifier | https://ror.org/03yxnpp24 | |
dc.type.version | info:eu-repo/semantics/publishedVersion | es |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es |
dc.relation.projectID | BFU2014-52299-P | es |
dc.relation.projectID | RD12/0019-0022 | es |
dc.relation.publisherversion | http://dx.doi.org/10.3324/haematol.2016.155812 | es |
dc.identifier.doi | 10.3324/haematol.2016.155812 | es |
idus.format.extent | 8 p. | es |
dc.journaltitle | Haematologica | es |
dc.publication.volumen | 102 | es |
dc.publication.issue | 4 | es |
dc.publication.initialPage | 647 | es |
dc.publication.endPage | 655 | es |
dc.contributor.funder | Ministerio de Economía. España | |
dc.contributor.funder | Instituto de Salud Carlos III | |